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Protein

Thymidine kinase

Gene

TK

Organism
Amsacta moorei entomopoxvirus (AmEPV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + thymidine = ADP + thymidine 5'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei85 – 851Proton acceptorSequence Analysis
Binding sitei117 – 1171Substrate; via amide nitrogenBy similarity
Metal bindingi142 – 1421ZincBy similarity
Metal bindingi145 – 1451ZincBy similarity
Metal bindingi174 – 1741ZincBy similarity
Metal bindingi177 – 1771ZincBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 158ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. thymidine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

DNA synthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidine kinase (EC:2.7.1.21)
Gene namesi
Name:TK
Ordered Locus Names:AMV016
ORF Names:Q2
OrganismiAmsacta moorei entomopoxvirus (AmEPV)
Taxonomic identifieri28321 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeEntomopoxvirinaeBetaentomopoxvirus
Virus hostiAmsacta [TaxID: 340055]
ProteomesiUP000000872 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 182182Thymidine kinasePRO_0000174942Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP28852.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1655Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the thymidine kinase family.Curated

Phylogenomic databases

KOiK00857.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28852-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIELIIGPM FSGKTTELMR KINRYILSNQ KCVIITHNID NRFINKNIIN
60 70 80 90 100
HDGNILNKEY LYIKTNNLIN EINIVDNYDI IGIDECQFFE ENDLEQFCDK
110 120 130 140 150
MANNKKKVIV AGLNCDFNRN IFNSISKLIP KVEKIKKLQA ICQFCYKDAS
160 170 180
FTIKKHNKNQ IIEIGGQDLY VPVCRLCYNN SY
Length:182
Mass (Da):21,241
Last modified:November 30, 1992 - v1
Checksum:i982719AFC0DF8D2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10679 Genomic DNA. Translation: BAA01525.1.
M80924 Genomic DNA. Translation: AAA42386.1.
AF250284 Genomic DNA. Translation: AAG02722.1.
PIRiB40818. KIVZAM.
RefSeqiNP_064798.1. NC_002520.1.

Genome annotation databases

GeneIDi1494606.
KEGGivg:1494606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10679 Genomic DNA. Translation: BAA01525.1.
M80924 Genomic DNA. Translation: AAA42386.1.
AF250284 Genomic DNA. Translation: AAG02722.1.
PIRiB40818. KIVZAM.
RefSeqiNP_064798.1. NC_002520.1.

3D structure databases

ProteinModelPortaliP28852.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1494606.
KEGGivg:1494606.

Phylogenomic databases

KOiK00857.

Family and domain databases

InterProiIPR027417. P-loop_NTPase.
IPR001267. Thymidine_kinase.
IPR020633. Thymidine_kinase_CS.
[Graphical view]
PANTHERiPTHR11441. PTHR11441. 1 hit.
PfamiPF00265. TK. 1 hit.
[Graphical view]
PIRSFiPIRSF035805. TK_cell. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00603. TK_CELLULAR_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mapping and molecular characterization of a functional thymidine kinase from Amsacta moorei entomopoxvirus."
    Gruidl M.E., Hall R.L., Moyer R.W.
    Virology 186:507-516(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Comparison of the thymidine kinase genes from three entomopoxviruses."
    Lytvyn V., Fortin Y., Banville M., Arif B., Richardson C.
    J. Gen. Virol. 73:3235-3240(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses."
    Bawden A.L., Glassberg K.J., Diggans J., Shaw R., Farmerie W., Moyer R.W.
    Virology 274:120-139(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiKITH_AMEPV
AccessioniPrimary (citable) accession number: P28852
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 1992
Last sequence update: November 30, 1992
Last modified: March 31, 2015
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.