P28852 (KITH_AMEPV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	TK
Ordered Locus Names:	AMV016
ORF Names:	Q2

Organism

Amsacta moorei entomopoxvirus (AmEPV) [Reference proteome]

Taxonomic identifier

28321 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Entomopoxvirinae › Betaentomopoxvirus

Virus host

Amsacta [TaxID: 340055]

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Thymidine kinase		PRO_0000174942
Regions
	Nucleotide binding	8 – 15	8	ATP By similarity
	Region	161 – 165	5	Substrate binding By similarity
Sites
	Active site	85	1	Proton acceptor Potential
	Metal binding	142	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Metal binding	174	1	Zinc By similarity
	Metal binding	177	1	Zinc By similarity
	Binding site	117	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28852 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 982719AFC0DF8D2D	FASTA	182	21,241
	10 20 30 40 50 60 MSIELIIGPM FSGKTTELMR KINRYILSNQ KCVIITHNID NRFINKNIIN HDGNILNKEY 70 80 90 100 110 120 LYIKTNNLIN EINIVDNYDI IGIDECQFFE ENDLEQFCDK MANNKKKVIV AGLNCDFNRN 130 140 150 160 170 180 IFNSISKLIP KVEKIKKLQA ICQFCYKDAS FTIKKHNKNQ IIEIGGQDLY VPVCRLCYNN SY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mapping and molecular characterization of a functional thymidine kinase from Amsacta moorei entomopoxvirus." Gruidl M.E., Hall R.L., Moyer R.W. Virology 186:507-516(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Comparison of the thymidine kinase genes from three entomopoxviruses." Lytvyn V., Fortin Y., Banville M., Arif B., Richardson C. J. Gen. Virol. 73:3235-3240(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete genomic sequence of the Amsacta moorei entomopoxvirus: analysis and comparison with other poxviruses." Bawden A.L., Glassberg K.J., Diggans J., Shaw R., Farmerie W., Moyer R.W. Virology 274:120-139(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10679 Genomic DNA. Translation: BAA01525.1. M80924 Genomic DNA. Translation: AAA42386.1. AF250284 Genomic DNA. Translation: AAG02722.1.
PIR	KIVZAM. B40818.
RefSeq	NP_064798.1. NC_002520.1.
3D structure databases
ProteinModelPortal	P28852.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1494606.
Phylogenomic databases
ProtClustDB	CLSP2509781.
Family and domain databases
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_AMEPV

Accession

Primary (citable) accession number: P28852

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program