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Protein

Ribonucleoside-diphosphate reductase large subunit

Gene

RIR1

Organism
Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.UniRule annotation

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.UniRule annotation

Pathwayi: DNA replication

This protein is involved in the pathway DNA replication, which is part of Genetic information processing.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway DNA replication and in Genetic information processing.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208SubstrateUniRule annotation1
Sitei224Important for hydrogen atom transferUniRule annotation1
Binding sitei254Substrate; via amide nitrogenUniRule annotation1
Active sitei436Proton acceptorUniRule annotation1
Active sitei438Cysteine radical intermediateUniRule annotation1
Active sitei440Proton acceptorUniRule annotation1
Sitei453Important for hydrogen atom transferUniRule annotation1
Sitei765Important for electron transferUniRule annotation1
Sitei766Important for electron transferUniRule annotation1
Sitei785Interacts with thioredoxin/glutaredoxinUniRule annotation1
Sitei788Interacts with thioredoxin/glutaredoxinUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processDNA replication, Viral latency, Viral reactivation from latency
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunitUniRule annotation (EC:1.17.4.1UniRule annotation)
Short name:
R1UniRule annotation
Alternative name(s):
Ribonucleotide reductase large subunitUniRule annotation
Gene namesi
Name:RIR1UniRule annotation
Ordered Locus Names:21
OrganismiEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Taxonomic identifieri31520 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
Proteomesi
  • UP000001189 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872411 – 790Ribonucleoside-diphosphate reductase large subunitAdd BLAST790

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi224 ↔ 453Redox-activeUniRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP28846

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP28846
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni223 – 224Substrate bindingUniRule annotation2
Regioni436 – 440Substrate bindingUniRule annotation5
Regioni621 – 625Substrate bindingUniRule annotation5

Sequence similaritiesi

Belongs to the ribonucleoside diphosphate reductase large chain family.UniRule annotation

Phylogenomic databases

OrthoDBiVOG0900001D

Family and domain databases

HAMAPiMF_04026 HSV_RIR1, 1 hit
InterProiView protein in InterPro
IPR034717 HSV_RIR1
IPR013346 NrdE_NrdA
IPR000788 RNR_lg_C
IPR013509 RNR_lsu_N
PfamiView protein in Pfam
PF02867 Ribonuc_red_lgC, 1 hit
PF00317 Ribonuc_red_lgN, 1 hit
PRINTSiPR01183 RIBORDTASEM1
TIGRFAMsiTIGR02506 NrdE_NrdA, 1 hit
PROSITEiView protein in PROSITE
PS00089 RIBORED_LARGE, 1 hit

Sequencei

Sequence statusi: Complete.

P28846-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALNFLQSDC PLAIIQDVIS RVDAISDYGY ANELSTTLPP RPSRSQVLEY
60 70 80 90 100
ITRVVDTLKP RCRVDERLYV VCGELVHLRI RTRNVEDLKY WLNSTEIALN
110 120 130 140 150
EIVEKDILDH LDFIQRTLHA FESSEYRELC ALGLQSALKY EEMYLAKMRG
160 170 180 190 200
GRIESMGQFF LRLATTATHY TMEEPAMARV LVSGEVGWTY IFKAYFTALA
210 220 230 240 250
GQVLIPATPI MLFGGRDCGS LASCYLLNPR VTDMNSAMLA LMEEAGPILC
260 270 280 290 300
NRGGIGLSLQ RFNTPPKEGC SRGVMALLKL IDSMTMAINS DGERPTGVCV
310 320 330 340 350
YFEPWHADIR AILNMRGMLA RDETVRCDNI FACMWTPDLF FDRYQRYLDG
360 370 380 390 400
ESGVMWTLFD DTASHLCHMY GKEFEEEYER LEQCGFGVDS IPIQDMAFII
410 420 430 440 450
VRSAVMTGSP FLMFKDACNK HYHFDLRRKG AIMGSNLCTE IIQHADETQN
460 470 480 490 500
GVCNLASINL PKCLAIPPPH TAGVPYFDFA ALGRAAATAT IFVNSMMRAG
510 520 530 540 550
TYPTVKSQRG VDENRSLGLG IQGLHTAFLM LDLDMASPEA RQLNKQIAER
560 570 580 590 600
LLLNSMKASA TLCRLGMKPF KGFEDSKYSL GELPFDSYPG VTLANRNAWR
610 620 630 640 650
RLRTEIKQHG LYNSQFVAYM PTVSSSQVTE SSEGFSPVYT NLFSKVTATG
660 670 680 690 700
EVLRPNLLLM RTIRSIFPRE CARLQALSTL EMAQWSVVGA FGDLPVGHPL
710 720 730 740 750
SKFKTAFEYD QRTLIDMCAD RAPFVDQSQS MSLFITEPAD GKLPASKIMS
760 770 780 790
LLVHAYKRGL KTGMYYCKIK KATNNGVFVG GDLVCTSCSL
Length:790
Mass (Da):88,399
Last modified:December 1, 1992 - v1
Checksum:iD8A21677716F5844
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY665713 Genomic DNA Translation: AAT67278.1
PIRiD36797 WMBEA2
RefSeqiYP_053066.1, NC_001491.2

Genome annotation databases

GeneIDi1487540
KEGGivg:1487540

Similar proteinsi

Entry informationi

Entry nameiRIR1_EHV1B
AccessioniPrimary (citable) accession number: P28846
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
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Main funding by: National Institutes of Health