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P28846

- RIR1_EHV1B

UniProt

P28846 - RIR1_EHV1B

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Protein
Ribonucleoside-diphosphate reductase large subunit
Gene
21
Organism
Equine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Ribonucleoside-diphosphate reductase holoenzyme provides the precursors necessary for viral DNA synthesis. Allows virus growth in non-dividing cells, as well as reactivation from latency in infected hosts. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei208 – 2081Substrate By similarity
Sitei224 – 2241Important for hydrogen atom transfer By similarity
Binding sitei254 – 2541Substrate; via amide nitrogen By similarity
Active sitei436 – 4361Proton acceptor By similarity
Active sitei438 – 4381Cysteine radical intermediate By similarity
Active sitei440 – 4401Proton acceptor By similarity
Sitei453 – 4531Important for hydrogen atom transfer By similarity
Sitei765 – 7651Important for electron transfer By similarity
Sitei766 – 7661Important for electron transfer By similarity
Sitei785 – 7851Interacts with thioredoxin/glutaredoxin By similarity
Sitei788 – 7881Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase large subunit (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase large subunit
Gene namesi
Name:21
OrganismiEquine herpesvirus 1 (strain Ab4p) (EHV-1) (Equine abortion virus)
Taxonomic identifieri31520 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeAlphaherpesvirinaeVaricellovirus
Virus hostiEquus caballus (Horse) [TaxID: 9796]
ProteomesiUP000001189: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 790790Ribonucleoside-diphosphate reductase large subunit
PRO_0000187241Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi224 ↔ 453Redox-active By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP28846.

Expressioni

Keywords - Developmental stagei

Early protein

Interactioni

Subunit structurei

Heterotetramer composed of a homodimer of the large subunit (R1) and a homodimer of the small subunit (R2). Larger multisubunit protein complex are also active, composed of (R1)n(R2)n By similarity.

Structurei

3D structure databases

ProteinModelPortaliP28846.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni223 – 2242Substrate binding By similarity
Regioni436 – 4405Substrate binding By similarity
Regioni621 – 6255Substrate binding By similarity

Sequence similaritiesi

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28846-1 [UniParc]FASTAAdd to Basket

« Hide

MALNFLQSDC PLAIIQDVIS RVDAISDYGY ANELSTTLPP RPSRSQVLEY    50
ITRVVDTLKP RCRVDERLYV VCGELVHLRI RTRNVEDLKY WLNSTEIALN 100
EIVEKDILDH LDFIQRTLHA FESSEYRELC ALGLQSALKY EEMYLAKMRG 150
GRIESMGQFF LRLATTATHY TMEEPAMARV LVSGEVGWTY IFKAYFTALA 200
GQVLIPATPI MLFGGRDCGS LASCYLLNPR VTDMNSAMLA LMEEAGPILC 250
NRGGIGLSLQ RFNTPPKEGC SRGVMALLKL IDSMTMAINS DGERPTGVCV 300
YFEPWHADIR AILNMRGMLA RDETVRCDNI FACMWTPDLF FDRYQRYLDG 350
ESGVMWTLFD DTASHLCHMY GKEFEEEYER LEQCGFGVDS IPIQDMAFII 400
VRSAVMTGSP FLMFKDACNK HYHFDLRRKG AIMGSNLCTE IIQHADETQN 450
GVCNLASINL PKCLAIPPPH TAGVPYFDFA ALGRAAATAT IFVNSMMRAG 500
TYPTVKSQRG VDENRSLGLG IQGLHTAFLM LDLDMASPEA RQLNKQIAER 550
LLLNSMKASA TLCRLGMKPF KGFEDSKYSL GELPFDSYPG VTLANRNAWR 600
RLRTEIKQHG LYNSQFVAYM PTVSSSQVTE SSEGFSPVYT NLFSKVTATG 650
EVLRPNLLLM RTIRSIFPRE CARLQALSTL EMAQWSVVGA FGDLPVGHPL 700
SKFKTAFEYD QRTLIDMCAD RAPFVDQSQS MSLFITEPAD GKLPASKIMS 750
LLVHAYKRGL KTGMYYCKIK KATNNGVFVG GDLVCTSCSL 790
Length:790
Mass (Da):88,399
Last modified:December 1, 1992 - v1
Checksum:iD8A21677716F5844
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY665713 Genomic DNA. Translation: AAT67278.1.
PIRiD36797. WMBEA2.
RefSeqiYP_053066.1. NC_001491.2.

Genome annotation databases

GeneIDi1487540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY665713 Genomic DNA. Translation: AAT67278.1 .
PIRi D36797. WMBEA2.
RefSeqi YP_053066.1. NC_001491.2.

3D structure databases

ProteinModelPortali P28846.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P28846.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1487540.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  2. "Tinkering with a viral ribonucleotide reductase."
    Lembo D., Brune W.
    Trends Biochem. Sci. 34:25-32(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry nameiRIR1_EHV1B
AccessioniPrimary (citable) accession number: P28846
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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