Reviewed, UniProtKB/Swiss-Prot P28842 (SUBT_BACS9)

Names and origin

Protein names

Recommended name:
    Subtilisin
    EC=3.4.21.62

Gene names

Name:

sub1

Organism

Bacillus sp. (strain TA39)

Taxonomic identifier

29336 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
Catalytic activity	Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
Cofactor	Binds 1 calcium ion per subunit Potential.
Subcellular location	Secreted.
Miscellaneous	Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.
Sequence similarities	Belongs to the peptidase S8 family.
biophysicochemical properties	Temperature dependence: Still active at temperatures close to 0 degrees Celsius. Thermolabile.

Ontologies

Keywords
Biological process	Sporulation
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Propeptide	27 – 111	85	Potential		PRO_0000027181
	Chain	112 – 420	309	Subtilisin		PRO_0000027182
Sites
	Active site	145	1	Charge relay system By similarity
	Active site	182	1	Charge relay system By similarity
	Active site	360	1	Charge relay system By similarity
	Metal binding	115	1	Calcium Potential
	Metal binding	154	1	Calcium Potential

Sequences

	Sequence		Length	Mass (Da)
	P28842-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: AE4F121BD32B26EC	FASTA	420	44,087
	10 20 30 40 50 60 MKRSGKIFTT AMLAVTLMMP AMGVSANEGN AAAEGNEKFR VLVDSVDQKN LKNAKQQYGV 70 80 90 100 110 120 HWDFAGEGFT TDMNEKQFNA LKKNKNLTVE KVPELEIATA TDKPEALYNA MAASQSTPWG 130 140 150 160 170 180 IKAIYNNSSI TQTSGGGGIN IAVLDTGVNT NHPDLRNNVE QCKDFTVGTT YTNNSCTDRQ 190 200 210 220 230 240 GHGTHVAGSA LADGGTGNGV YGVAPDADLW AYKVLGDDGS GYADDIAAAI RHAGDQATAL 250 260 270 280 290 300 NTKVVINMSL GSSGESSLIT NAVNYSYNKG VLIIAAAGNS GPYQGSIGYP GALVNAVAVA 310 320 330 340 350 360 ALENKVENGT YRVADFSSRG YSWTDGDYAI QKGDVEISAP GAAIYSTWFD GGYATISGTS 370 380 390 400 410 420 MASPHAAGLA AKIWAQYPSA SNVDVRGELQ YRAYENDILS GYYAGYGDDF ASGFGFATVQ

References

[1]	"Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39." Narinx E., Davail S., Feller G., Gerday C. Biochim. Biophys. Acta 1131:111-113(1992) [PubMed: 1581352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X62369 Genomic DNA. Translation: CAA44227.1.
PIR	S23407.
3D structure databases
HSSP	HSSP built from PDB template 1EA7 based on UniProtKB Q9S3L6.
SMR	P28842. Positions 112-420.
ModBase	Search...
Protein family/group databases
MEROPS	S08.113.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SUBT_BACS9

Accession

Primary (citable) accession number: P28842

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)