P28842 (SUBT_BACS9) Reviewed, UniProtKB/Swiss-Prot
Last modified
November 16, 2011.
Version 74.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Subtilisin EC=3.4.21.62 | ||
| Gene names |
| ||
| Organism | Bacillus sp. (strain TA39) | ||
| Taxonomic identifier | 29336 [NCBI] | ||
| Taxonomic lineage | Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus |
Protein attributes
| Sequence length | 420 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. |
| Catalytic activity | Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides. |
| Cofactor | Binds 1 calcium ion per subunit Potential. |
| Subcellular location | |
| Miscellaneous | Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation. |
| Sequence similarities | Belongs to the peptidase S8 family. |
| Biophysicochemical properties | Temperature dependence: Still active at temperatures close to 0 degrees Celsius. Thermolabile. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Sporulation |
| Cellular component | Secreted |
| Domain | Signal |
| Ligand | Calcium Metal-binding |
| Molecular function | Hydrolase Protease Serine protease |
| PTM | Zymogen |
| Gene Ontology (GO) | |
| Biological process | proteolysis Inferred from electronic annotation. Source: UniProtKB-KW sporulation resulting in formation of a cellular sporeInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 31 | 31 | Potential | ||||||
| Propeptide | 32 – 111 | 80 | Potential | PRO_0000027181 | |||||
| Chain | 112 – 420 | 309 | Subtilisin | PRO_0000027182 | |||||
Sites | |||||||||
| Active site | 145 | 1 | Charge relay system By similarity | ||||||
| Active site | 182 | 1 | Charge relay system By similarity | ||||||
| Active site | 360 | 1 | Charge relay system By similarity | ||||||
| Metal binding | 115 | 1 | Calcium Potential | ||||||
| Metal binding | 154 | 1 | Calcium Potential | ||||||
Sequences
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References
| [1] | "Nucleotide and derived amino acid sequence of the subtilisin from the antarctic psychrotroph Bacillus TA39." Narinx E., Davail S., Feller G., Gerday C. Biochim. Biophys. Acta 1131:111-113(1992) [PubMed: 1581352] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X62369 Genomic DNA. Translation: CAA44227.1. |
| PIR | S23407. |
3D structure databases | |
| ProteinModelPortal | P28842. |
| SMR | P28842. Positions 112-420. |
| ModBase | Search... |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR000209. Peptidase_S8/S53. IPR023827. Peptidase_S8_Asp-AS. IPR022398. Peptidase_S8_His-AS. IPR023828. Peptidase_S8_Ser-AS. IPR015500. Peptidase_S8_subtilisin-rel. [Graphical view] |
| Gene3D | G3DSA:3.40.50.200. Pept_S8_S53. 1 hit. |
| PANTHER | PTHR10795. SubtilSerProt. 1 hit. |
| Pfam | PF00082. Peptidase_S8. 1 hit. [Graphical view] |
| PRINTS | PR00723. SUBTILISIN. |
| SUPFAM | SSF52743. Pept_S8_S53. 1 hit. |
| PROSITE | PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | SUBT_BACS9 | ||||||||
| Accession | Primary (citable) accession number: P28842 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Prokaryotic Protein Annotation Program | ||||||||
Relevant documents
| Peptidase families Classification of peptidase families and list of entries |
| SIMILARITY comments Index of protein domains and families |

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