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Protein

Neuroendocrine convertase 2

Gene

Pcsk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells (By similarity).By similarity

Catalytic activityi

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei166Charge relay systemBy similarity1
Active sitei207Charge relay systemBy similarity1
Active sitei383Charge relay systemBy similarity1

GO - Molecular functioni

  • protein complex binding Source: RGD
  • serine-type endopeptidase activity Source: BHF-UCL

GO - Biological processi

  • enkephalin processing Source: Ensembl
  • insulin processing Source: Ensembl
  • islet amyloid polypeptide processing Source: BHF-UCL
  • nervous system development Source: Ensembl
  • peptide hormone processing Source: RGD
  • protein autoprocessing Source: Ensembl
  • proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-RNO-264876. Insulin processing.

Protein family/group databases

MEROPSiS08.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 2 (EC:3.4.21.94)
Short name:
NEC 2
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name:
PC2
Gene namesi
Name:Pcsk2
Synonyms:Nec-2, Nec2, Rpc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi3273. Pcsk2.

Subcellular locationi

GO - Cellular componenti

  • dendrite Source: RGD
  • extracellular space Source: GO_Central
  • membrane Source: Ensembl
  • neuronal cell body Source: RGD
  • perikaryon Source: RGD
  • transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002707125 – 108Sequence analysisAdd BLAST84
ChainiPRO_0000027072109 – 637Neuroendocrine convertase 2Add BLAST529

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi224 ↔ 375By similarity
Disulfide bondi316 ↔ 346By similarity
Glycosylationi374N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi467 ↔ 493By similarity
Glycosylationi513N-linked (GlcNAc...)Sequence analysis1
Glycosylationi523N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28841.
PRIDEiP28841.

PTM databases

PhosphoSitePlusiP28841.
UniCarbKBiP28841.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005438.
GenevisibleiP28841. RN.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Scg5P276824EBI-988244,EBI-988232

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiP28841. 1 interactor.
STRINGi10116.ENSRNOP00000007249.

Structurei

3D structure databases

ProteinModelPortaliP28841.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini161 – 452Peptidase S8Add BLAST292

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
KOG3526. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28841.
KOiK01360.
OMAiDAFPYPR.
OrthoDBiEOG091G05HI.
PhylomeDBiP28841.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28841-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA
60 70 80 90 100
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ
110 120 130 140 150
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA
160 170 180 190 200
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NSDASYDFSS NDPYPYPRYT
210 220 230 240 250
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE
260 270 280 290 300
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG
310 320 330 340 350
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL
360 370 380 390 400
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN
410 420 430 440 450
VDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA
460 470 480 490 500
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLQT NACEGKENFV
510 520 530 540 550
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK
560 570 580 590 600
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID
610 620 630
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN
Length:637
Mass (Da):70,753
Last modified:December 1, 1992 - v1
Checksum:i45DCB85B2CDA4B19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti320G → GI in AAA41477 (PubMed:1954888).Curated1
Sequence conflicti342Y → H in AAA41477 (PubMed:1954888).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76706 mRNA. Translation: AAA40946.1.
M83746 mRNA. Translation: AAA41477.1.
PIRiB41556. KXRTC2.
RefSeqiNP_036878.1. NM_012746.1.
UniGeneiRn.89052.

Genome annotation databases

EnsembliENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
GeneIDi25121.
KEGGirno:25121.
UCSCiRGD:3273. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76706 mRNA. Translation: AAA40946.1.
M83746 mRNA. Translation: AAA41477.1.
PIRiB41556. KXRTC2.
RefSeqiNP_036878.1. NM_012746.1.
UniGeneiRn.89052.

3D structure databases

ProteinModelPortaliP28841.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28841. 1 interactor.
STRINGi10116.ENSRNOP00000007249.

Protein family/group databases

MEROPSiS08.073.

PTM databases

PhosphoSitePlusiP28841.
UniCarbKBiP28841.

Proteomic databases

PaxDbiP28841.
PRIDEiP28841.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
GeneIDi25121.
KEGGirno:25121.
UCSCiRGD:3273. rat.

Organism-specific databases

CTDi5126.
RGDi3273. Pcsk2.

Phylogenomic databases

eggNOGiKOG3525. Eukaryota.
KOG3526. Eukaryota.
COG1404. LUCA.
COG4935. LUCA.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28841.
KOiK01360.
OMAiDAFPYPR.
OrthoDBiEOG091G05HI.
PhylomeDBiP28841.
TreeFamiTF314277.

Enzyme and pathway databases

ReactomeiR-RNO-264876. Insulin processing.

Miscellaneous databases

PROiP28841.

Gene expression databases

BgeeiENSRNOG00000005438.
GenevisibleiP28841. RN.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.30.70.850. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR002884. PrprotnconvertsP.
IPR032815. S8_pro-domain.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF16470. S8_pro-domain. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEC2_RAT
AccessioniPrimary (citable) accession number: P28841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.