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P28841

- NEC2_RAT

UniProt

P28841 - NEC2_RAT

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Protein

Neuroendocrine convertase 2

Gene

Pcsk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells (By similarity).By similarity

Catalytic activityi

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Charge relay systemBy similarity
Active sitei207 – 2071Charge relay systemBy similarity
Active sitei383 – 3831Charge relay systemBy similarity

GO - Molecular functioni

  1. protein complex binding Source: RGD
  2. serine-type endopeptidase activity Source: BHF-UCL

GO - Biological processi

  1. enkephalin processing Source: Ensembl
  2. insulin processing Source: Ensembl
  3. islet amyloid polypeptide processing Source: BHF-UCL
  4. nervous system development Source: Ensembl
  5. peptide hormone processing Source: RGD
  6. protein autoprocessing Source: Ensembl
  7. proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_229884. Insulin processing.

Protein family/group databases

MEROPSiS08.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 2 (EC:3.4.21.94)
Short name:
NEC 2
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name:
PC2
Gene namesi
Name:Pcsk2
Synonyms:Nec-2, Nec2, Rpc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi3273. Pcsk2.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle
Note: Localized in the secretion granules.

GO - Cellular componenti

  1. cytoplasmic vesicle Source: UniProtKB-KW
  2. dendrite Source: RGD
  3. extracellular space Source: RefGenome
  4. membrane Source: Ensembl
  5. neuronal cell body Source: RGD
  6. perikaryon Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 10884Sequence AnalysisPRO_0000027071Add
BLAST
Chaini109 – 637529Neuroendocrine convertase 2PRO_0000027072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi224 ↔ 375By similarity
Disulfide bondi316 ↔ 346By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi467 ↔ 493By similarity
Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28841.
PRIDEiP28841.

Expressioni

Gene expression databases

GenevestigatoriP28841.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Scg5P276824EBI-988244,EBI-988232

Protein-protein interaction databases

IntActiP28841. 1 interaction.
STRINGi10116.ENSRNOP00000007249.

Structurei

3D structure databases

ProteinModelPortaliP28841.
SMRiP28841. Positions 119-596.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini161 – 452292Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28841.
KOiK01360.
OMAiWLLEATF.
OrthoDBiEOG7BW0JD.
PhylomeDBiP28841.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28841-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA
60 70 80 90 100
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ
110 120 130 140 150
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA
160 170 180 190 200
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NSDASYDFSS NDPYPYPRYT
210 220 230 240 250
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE
260 270 280 290 300
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG
310 320 330 340 350
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL
360 370 380 390 400
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN
410 420 430 440 450
VDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA
460 470 480 490 500
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLQT NACEGKENFV
510 520 530 540 550
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK
560 570 580 590 600
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID
610 620 630
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN
Length:637
Mass (Da):70,753
Last modified:December 1, 1992 - v1
Checksum:i45DCB85B2CDA4B19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201G → GI in AAA41477. (PubMed:1954888)Curated
Sequence conflicti342 – 3421Y → H in AAA41477. (PubMed:1954888)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76706 mRNA. Translation: AAA40946.1.
M83746 mRNA. Translation: AAA41477.1.
PIRiB41556. KXRTC2.
RefSeqiNP_036878.1. NM_012746.1.
UniGeneiRn.89052.

Genome annotation databases

EnsembliENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
GeneIDi25121.
KEGGirno:25121.
UCSCiRGD:3273. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76706 mRNA. Translation: AAA40946.1 .
M83746 mRNA. Translation: AAA41477.1 .
PIRi B41556. KXRTC2.
RefSeqi NP_036878.1. NM_012746.1.
UniGenei Rn.89052.

3D structure databases

ProteinModelPortali P28841.
SMRi P28841. Positions 119-596.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P28841. 1 interaction.
STRINGi 10116.ENSRNOP00000007249.

Protein family/group databases

MEROPSi S08.073.

Proteomic databases

PaxDbi P28841.
PRIDEi P28841.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007249 ; ENSRNOP00000007249 ; ENSRNOG00000005438 .
GeneIDi 25121.
KEGGi rno:25121.
UCSCi RGD:3273. rat.

Organism-specific databases

CTDi 5126.
RGDi 3273. Pcsk2.

Phylogenomic databases

eggNOGi COG4935.
GeneTreei ENSGT00750000117358.
HOGENOMi HOG000192536.
HOVERGENi HBG008705.
InParanoidi P28841.
KOi K01360.
OMAi WLLEATF.
OrthoDBi EOG7BW0JD.
PhylomeDBi P28841.
TreeFami TF314277.

Enzyme and pathway databases

Reactomei REACT_229884. Insulin processing.

Miscellaneous databases

NextBioi 605503.
PROi P28841.

Gene expression databases

Genevestigatori P28841.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view ]
PANTHERi PTHR10795. PTHR10795. 1 hit.
Pfami PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view ]
PRINTSi PR00723. SUBTILISIN.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
    Bloomquist B.T., Eipper B.A., Mains R.E.
    Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
    Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
    Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNEC2_RAT
AccessioniPrimary (citable) accession number: P28841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 26, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3