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P28841

- NEC2_RAT

UniProt

P28841 - NEC2_RAT

Protein

Neuroendocrine convertase 2

Gene

Pcsk2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.By similarity

    Catalytic activityi

    Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei166 – 1661Charge relay systemBy similarity
    Active sitei207 – 2071Charge relay systemBy similarity
    Active sitei383 – 3831Charge relay systemBy similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein complex binding Source: RGD
    3. serine-type endopeptidase activity Source: BHF-UCL

    GO - Biological processi

    1. enkephalin processing Source: Ensembl
    2. insulin processing Source: Ensembl
    3. islet amyloid polypeptide processing Source: BHF-UCL
    4. nervous system development Source: Ensembl
    5. peptide hormone processing Source: RGD
    6. protein autoprocessing Source: Ensembl
    7. proteolysis Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS08.073.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine convertase 2 (EC:3.4.21.94)
    Short name:
    NEC 2
    Alternative name(s):
    KEX2-like endoprotease 2
    Prohormone convertase 2
    Proprotein convertase 2
    Short name:
    PC2
    Gene namesi
    Name:Pcsk2
    Synonyms:Nec-2, Nec2, Rpc2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 3

    Organism-specific databases

    RGDi3273. Pcsk2.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle
    Note: Localized in the secretion granules.

    GO - Cellular componenti

    1. dendrite Source: RGD
    2. extracellular space Source: RefGenome
    3. membrane Source: Ensembl
    4. neuronal cell body Source: RGD
    5. perikaryon Source: RGD
    6. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 10884Sequence AnalysisPRO_0000027071Add
    BLAST
    Chaini109 – 637529Neuroendocrine convertase 2PRO_0000027072Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi224 ↔ 375By similarity
    Disulfide bondi316 ↔ 346By similarity
    Glycosylationi374 – 3741N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi467 ↔ 493By similarity
    Glycosylationi513 – 5131N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi523 – 5231N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP28841.
    PRIDEiP28841.

    Expressioni

    Gene expression databases

    GenevestigatoriP28841.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Scg5P276824EBI-988244,EBI-988232

    Protein-protein interaction databases

    IntActiP28841. 1 interaction.
    STRINGi10116.ENSRNOP00000007249.

    Structurei

    3D structure databases

    ProteinModelPortaliP28841.
    SMRiP28841. Positions 119-596.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini161 – 452292Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family. Furin subfamily.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4935.
    GeneTreeiENSGT00750000117358.
    HOGENOMiHOG000192536.
    HOVERGENiHBG008705.
    InParanoidiP28841.
    KOiK01360.
    OMAiWLLEATF.
    OrthoDBiEOG7BW0JD.
    PhylomeDBiP28841.
    TreeFamiTF314277.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28841-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA    50
    EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ 100
    EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA 150
    WELGYTGKGV TIGIMDDGID YLHPDLAYNY NSDASYDFSS NDPYPYPRYT 200
    DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE 250
    ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 300
    SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL 350
    ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN 400
    VDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA 450
    MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLQT NACEGKENFV 500
    RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK 550
    WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 600
    QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN 637
    Length:637
    Mass (Da):70,753
    Last modified:December 1, 1992 - v1
    Checksum:i45DCB85B2CDA4B19
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti320 – 3201G → GI in AAA41477. (PubMed:1954888)Curated
    Sequence conflicti342 – 3421Y → H in AAA41477. (PubMed:1954888)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76706 mRNA. Translation: AAA40946.1.
    M83746 mRNA. Translation: AAA41477.1.
    PIRiB41556. KXRTC2.
    RefSeqiNP_036878.1. NM_012746.1.
    UniGeneiRn.89052.

    Genome annotation databases

    EnsembliENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
    GeneIDi25121.
    KEGGirno:25121.
    UCSCiRGD:3273. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76706 mRNA. Translation: AAA40946.1 .
    M83746 mRNA. Translation: AAA41477.1 .
    PIRi B41556. KXRTC2.
    RefSeqi NP_036878.1. NM_012746.1.
    UniGenei Rn.89052.

    3D structure databases

    ProteinModelPortali P28841.
    SMRi P28841. Positions 119-596.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P28841. 1 interaction.
    STRINGi 10116.ENSRNOP00000007249.

    Protein family/group databases

    MEROPSi S08.073.

    Proteomic databases

    PaxDbi P28841.
    PRIDEi P28841.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000007249 ; ENSRNOP00000007249 ; ENSRNOG00000005438 .
    GeneIDi 25121.
    KEGGi rno:25121.
    UCSCi RGD:3273. rat.

    Organism-specific databases

    CTDi 5126.
    RGDi 3273. Pcsk2.

    Phylogenomic databases

    eggNOGi COG4935.
    GeneTreei ENSGT00750000117358.
    HOGENOMi HOG000192536.
    HOVERGENi HBG008705.
    InParanoidi P28841.
    KOi K01360.
    OMAi WLLEATF.
    OrthoDBi EOG7BW0JD.
    PhylomeDBi P28841.
    TreeFami TF314277.

    Miscellaneous databases

    NextBioi 605503.
    PROi P28841.

    Gene expression databases

    Genevestigatori P28841.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
      Bloomquist B.T., Eipper B.A., Mains R.E.
      Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
      Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
      Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiNEC2_RAT
    AccessioniPrimary (citable) accession number: P28841
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3