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P28841

- NEC2_RAT

UniProt

P28841 - NEC2_RAT

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Protein
Neuroendocrine convertase 2
Gene
Pcsk2, Nec-2, Nec2, Rpc2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Responsible for the release of glucagon from proglucagon in pancreatic A cells By similarity.

Catalytic activityi

Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei166 – 1661Charge relay system By similarity
Active sitei207 – 2071Charge relay system By similarity
Active sitei383 – 3831Charge relay system By similarity

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. protein complex binding Source: RGD
  3. serine-type endopeptidase activity Source: BHF-UCL
Complete GO annotation...

GO - Biological processi

  1. enkephalin processing Source: Ensembl
  2. insulin processing Source: Ensembl
  3. islet amyloid polypeptide processing Source: BHF-UCL
  4. nervous system development Source: Ensembl
  5. peptide hormone processing Source: RGD
  6. protein autoprocessing Source: Ensembl
  7. proteolysis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS08.073.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 2 (EC:3.4.21.94)
Short name:
NEC 2
Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name:
PC2
Gene namesi
Name:Pcsk2
Synonyms:Nec-2, Nec2, Rpc2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 3

Organism-specific databases

RGDi3273. Pcsk2.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle
Note: Localized in the secretion granules.

GO - Cellular componenti

  1. dendrite Source: RGD
  2. extracellular space Source: RefGenome
  3. membrane Source: Ensembl
  4. neuronal cell body Source: RGD
  5. perikaryon Source: RGD
  6. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424 Reviewed prediction
Add
BLAST
Propeptidei25 – 10884 Reviewed prediction
PRO_0000027071Add
BLAST
Chaini109 – 637529Neuroendocrine convertase 2
PRO_0000027072Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi224 ↔ 375 By similarity
Disulfide bondi316 ↔ 346 By similarity
Glycosylationi374 – 3741N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi467 ↔ 493 By similarity
Glycosylationi513 – 5131N-linked (GlcNAc...) Reviewed prediction
Glycosylationi523 – 5231N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28841.
PRIDEiP28841.

Expressioni

Gene expression databases

GenevestigatoriP28841.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Scg5P276824EBI-988244,EBI-988232

Protein-protein interaction databases

IntActiP28841. 1 interaction.
STRINGi10116.ENSRNOP00000007249.

Structurei

3D structure databases

ProteinModelPortaliP28841.
SMRiP28841. Positions 119-596.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 414294Catalytic
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28841.
KOiK01360.
OMAiWLLEATF.
OrthoDBiEOG7BW0JD.
PhylomeDBiP28841.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28841-1 [UniParc]FASTAAdd to Basket

« Hide

MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA    50
EHGFGVRKLP FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ 100
EGFDRKKRGY RDINEIDINM NDPLFTKQWY LFNTGQADGT PGLDLNVAEA 150
WELGYTGKGV TIGIMDDGID YLHPDLAYNY NSDASYDFSS NDPYPYPRYT 200
DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML DQPFMTDIIE 250
ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 300
SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL 350
ASTFSNGRKR NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN 400
VDLTWRDMQH LTVLTSKRNQ LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA 450
MVKMAKDWKT VPERFHCVGG SVQNPEKIPP TGKLVLTLQT NACEGKENFV 500
RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR DDDSKVGFDK 550
WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 600
QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN 637
Length:637
Mass (Da):70,753
Last modified:December 1, 1992 - v1
Checksum:i45DCB85B2CDA4B19
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201G → GI in AAA41477. 1 Publication
Sequence conflicti342 – 3421Y → H in AAA41477. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76706 mRNA. Translation: AAA40946.1.
M83746 mRNA. Translation: AAA41477.1.
PIRiB41556. KXRTC2.
RefSeqiNP_036878.1. NM_012746.1.
UniGeneiRn.89052.

Genome annotation databases

EnsembliENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
GeneIDi25121.
KEGGirno:25121.
UCSCiRGD:3273. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76706 mRNA. Translation: AAA40946.1 .
M83746 mRNA. Translation: AAA41477.1 .
PIRi B41556. KXRTC2.
RefSeqi NP_036878.1. NM_012746.1.
UniGenei Rn.89052.

3D structure databases

ProteinModelPortali P28841.
SMRi P28841. Positions 119-596.
ModBasei Search...

Protein-protein interaction databases

IntActi P28841. 1 interaction.
STRINGi 10116.ENSRNOP00000007249.

Protein family/group databases

MEROPSi S08.073.

Proteomic databases

PaxDbi P28841.
PRIDEi P28841.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000007249 ; ENSRNOP00000007249 ; ENSRNOG00000005438 .
GeneIDi 25121.
KEGGi rno:25121.
UCSCi RGD:3273. rat.

Organism-specific databases

CTDi 5126.
RGDi 3273. Pcsk2.

Phylogenomic databases

eggNOGi COG4935.
GeneTreei ENSGT00750000117358.
HOGENOMi HOG000192536.
HOVERGENi HBG008705.
InParanoidi P28841.
KOi K01360.
OMAi WLLEATF.
OrthoDBi EOG7BW0JD.
PhylomeDBi P28841.
TreeFami TF314277.

Miscellaneous databases

NextBioi 605503.
PROi P28841.

Gene expression databases

Genevestigatori P28841.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view ]
PANTHERi PTHR10795. PTHR10795. 1 hit.
Pfami PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view ]
PRINTSi PR00723. SUBTILISIN.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
    Bloomquist B.T., Eipper B.A., Mains R.E.
    Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
    Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
    Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNEC2_RAT
AccessioniPrimary (citable) accession number: P28841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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