P28841 (NEC2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuroendocrine convertase 2
Short name=NEC 2
EC=3.4.21.94

Alternative name(s):
KEX2-like endoprotease 2
Prohormone convertase 2
Proprotein convertase 2
Short name=PC2

Gene names

Name:	Pcsk2
Synonyms:	Nec-2, Nec2, Rpc2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues.
Catalytic activity	Release of protein hormones and neuropeptides from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Subcellular location	Cytoplasmic vesicle › secretory vesicle. Note: Localized in the secretion granules.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	islet amyloid polypeptide processing Inferred from mutant phenotype. Source: BHF-UCL proteolysis Inferred from mutant phenotype. Source: RGD
Cellular component	extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome transport vesicle Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct serine-type endopeptidase activity Inferred from mutant phenotype. Source: BHF-UCL
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Scg5	P27682	4	EBI-988244,EBI-988232

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 108	84	Potential		PRO_0000027071
	Chain	109 – 637	529	Neuroendocrine convertase 2		PRO_0000027072
Regions
	Region	121 – 414	294	Catalytic
Sites
	Active site	166	1	Charge relay system By similarity
	Active site	207	1	Charge relay system By similarity
	Active site	383	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	374	1	N-linked (GlcNAc...) Potential
	Glycosylation	513	1	N-linked (GlcNAc...) Potential
	Glycosylation	523	1	N-linked (GlcNAc...) Potential
	Disulfide bond	224 ↔ 375		By similarity
	Disulfide bond	316 ↔ 346		By similarity
	Disulfide bond	467 ↔ 493		By similarity
Experimental info
	Sequence conflict	320	1	G → GI in AAA41477. Ref.2
	Sequence conflict	342	1	Y → H in AAA41477. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28841 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 45DCB85B2CDA4B19	FASTA	637	70,753
	10 20 30 40 50 60 MEGGCGSQWK AAGLLFCVMV FASAERPVFT NHFLVELHKD GEEEARQVAA EHGFGVRKLP 70 80 90 100 110 120 FAEGLYHFYH NGLAKAKRRR SLHHKRQLER DPRIKMALQQ EGFDRKKRGY RDINEIDINM 130 140 150 160 170 180 NDPLFTKQWY LFNTGQADGT PGLDLNVAEA WELGYTGKGV TIGIMDDGID YLHPDLAYNY 190 200 210 220 230 240 NSDASYDFSS NDPYPYPRYT DDWFNSHGTR CAGEVSAAAS NNICGVGVAY NSKVAGIRML 250 260 270 280 290 300 DQPFMTDIIE ASSISHMPQL IDIYSASWGP TDNGKTVDGP RELTLQAMAD GVNKGRGGKG 310 320 330 340 350 360 SIYVWASGDG GSYDDCNCDG YASSMWTISI NSAINDGRTA LYDESCSSTL ASTFSNGRKR 370 380 390 400 410 420 NPEAGVATTD LYGNCTLRHS GTSAAAPEAA GVFALALEAN VDLTWRDMQH LTVLTSKRNQ 430 440 450 460 470 480 LHDEVHQWRR NGVGLEFNHL FGYGVLDAGA MVKMAKDWKT VPERFHCVGG SVQNPEKIPP 490 500 510 520 530 540 TGKLVLTLQT NACEGKENFV RYLEHVQAVI TVNATRRGDL NINMTSPMGT KSILLSRRPR 550 560 570 580 590 600 DDDSKVGFDK WPFMTTHTWG EDARGTWTLE LGFVGSAPQK GLLKEWTLML HGTQSAPYID 610 620 630 QVVRDYQSKL AMSKKQELEE ELDEAVERSL QSILRKN

References

[1]	"Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA." Bloomquist B.T., Eipper B.A., Mains R.E. Mol. Endocrinol. 5:2014-2024(1991) [PubMed: 1791845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats." Hakes D.J., Birch N.P., Mezey A., Dixon J.E. Endocrinology 129:3053-3063(1991) [PubMed: 1954888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M76706 mRNA. Translation: AAA40946.1. M83746 mRNA. Translation: AAA41477.1.
IPI	IPI00204864.
PIR	KXRTC2. B41556.
RefSeq	NP_036878.1. NM_012746.1.
UniGene	Rn.89052.
3D structure databases
ProteinModelPortal	P28841.
SMR	P28841. Positions 119-596.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28841. 1 interaction.
STRING	P28841.
Protein family/group databases
MEROPS	S08.073.
Proteomic databases
PRIDE	P28841.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000007249; ENSRNOP00000007249; ENSRNOG00000005438.
GeneID	25121.
KEGG	rno:25121.
UCSC	NM_012746. rat.
Organism-specific databases
CTD	5126.
RGD	3273. Pcsk2.
Phylogenomic databases
eggNOG	roNOG07042.
GeneTree	ENSGT00600000084064.
HOVERGEN	HBG008705.
InParanoid	P28841.
OMA	NYNADAS.
OrthoDB	EOG46MBJ1.
PhylomeDB	P28841.
Gene expression databases
ArrayExpress	P28841.
Genevestigator	P28841.
GermOnline	ENSRNOG00000005438. Rattus norvegicus.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR000209. Peptidase_S8/S53. IPR023827. Peptidase_S8_Asp-AS. IPR022398. Peptidase_S8_His-AS. IPR023828. Peptidase_S8_Ser-AS. IPR015500. Peptidase_S8_subtilisin-rel. IPR009020. Prot_inh_propept. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
KO	K01360.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF52743. Pept_S8_S53. 1 hit. SSF54897. Prot_inh_propept. 1 hit.
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	605503.

Entry information

Entry name

NEC2_RAT

Accession

Primary (citable) accession number: P28841

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	November 16, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program