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Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin, insulin and AGRP.By similarity

Catalytic activityi

Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei167Charge relay systemBy similarity1
Active sitei208Charge relay systemBy similarity1
Active sitei382Charge relay systemBy similarity1

GO - Molecular functioni

  • chaperone binding Source: RGD
  • endopeptidase activity Source: RGD
  • insulin binding Source: RGD
  • serine-type endopeptidase activity Source: Reactome

GO - Biological processi

  • neurogenesis Source: RGD
  • pancreas development Source: RGD
  • peptide biosynthetic process Source: RGD
  • peptide hormone processing Source: RGD
  • pituitary gland development Source: RGD
  • positive regulation of protein secretion Source: RGD
  • protein autoprocessing Source: RGD
  • protein processing Source: RGD
  • proteolysis Source: RGD
  • response to axon injury Source: RGD
  • response to calcium ion Source: RGD
  • response to chlorate Source: RGD
  • response to drug Source: RGD
  • response to fatty acid Source: RGD
  • response to glucocorticoid Source: RGD
  • response to glucose Source: RGD
  • response to inorganic substance Source: RGD
  • response to interleukin-1 Source: RGD
  • response to lipopolysaccharide Source: RGD
  • response to morphine Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to peptide hormone Source: RGD

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium

Enzyme and pathway databases

ReactomeiR-RNO-209952 Peptide hormone biosynthesis
R-RNO-422085 Synthesis, secretion, and deacylation of Ghrelin

Protein family/group databases

MEROPSiS08.072

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 1 (EC:3.4.21.93)
Short name:
NEC 1
Alternative name(s):
Prohormone convertase 1
Proprotein convertase 1
Short name:
PC1
Gene namesi
Name:Pcsk1
Synonyms:Bdp, Nec-1, Nec1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi3272 Pcsk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002706328 – 110Sequence analysisAdd BLAST83
ChainiPRO_0000027064111 – 752Neuroendocrine convertase 1Add BLAST642

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi173N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi225 ↔ 374By similarity
Disulfide bondi317 ↔ 347By similarity
Glycosylationi401N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi467 ↔ 494By similarity
Glycosylationi645N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28840
PRIDEiP28840

PTM databases

PhosphoSitePlusiP28840

Expressioni

Gene expression databases

BgeeiENSRNOG00000011107
GenevisibleiP28840 RN

Interactioni

GO - Molecular functioni

  • chaperone binding Source: RGD
  • insulin binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015185

Structurei

3D structure databases

ProteinModelPortaliP28840
SMRiP28840
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini162 – 451Peptidase S8Add BLAST290
Domaini460 – 597P/Homo BPROSITE-ProRule annotationAdd BLAST138

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3525 Eukaryota
COG1404 LUCA
COG4935 LUCA
GeneTreeiENSGT00750000117358
HOGENOMiHOG000192536
HOVERGENiHBG008705
InParanoidiP28840
KOiK01359
OMAiFEPRALK
OrthoDBiEOG091G05HI
PhylomeDBiP28840
TreeFamiTF314277

Family and domain databases

CDDicd04059 Peptidases_S8_Protein_converta, 1 hit
Gene3Di2.60.120.2601 hit
3.30.70.8501 hit
3.40.50.2001 hit
InterProiView protein in InterPro
IPR008979 Galactose-bd-like_sf
IPR034182 Kexin/furin
IPR002884 P_dom
IPR000209 Peptidase_S8/S53_dom
IPR036852 Peptidase_S8/S53_dom_sf
IPR023827 Peptidase_S8_Asp-AS
IPR022398 Peptidase_S8_His-AS
IPR023828 Peptidase_S8_Ser-AS
IPR015500 Peptidase_S8_subtilisin-rel
IPR022005 Proho_convert
IPR032815 S8_pro-domain
IPR038466 S8_pro-domain_sf
PfamiView protein in Pfam
PF01483 P_proprotein, 1 hit
PF00082 Peptidase_S8, 1 hit
PF12177 Proho_convert, 1 hit
PF16470 S8_pro-domain, 1 hit
PRINTSiPR00723 SUBTILISIN
SUPFAMiSSF49785 SSF49785, 1 hit
SSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51829 P_HOMO_B, 1 hit
PS00136 SUBTILASE_ASP, 1 hit
PS00137 SUBTILASE_HIS, 1 hit
PS00138 SUBTILASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28840-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA
60 70 80 90 100
EELGYDLLGQ IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE
110 120 130 140 150
QQYEKERRKR SVPRDSALNL FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP
160 170 180 190 200
VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY
210 220 230 240 250
DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE
260 270 280 290 300
ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG
310 320 330 340 350
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST
360 370 380 390 400
LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP
410 420 430 440 450
NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV
460 470 480 490 500
DLADPRTWRN VPEKKECIIK DNNFEPRALK ANGEVIVEIP TRACEGQENA
510 520 530 540 550
INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN
560 570 580 590 600
WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH
610 620 630 640 650
MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS
660 670 680 690 700
VEDRRDEQVQ GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY
710 720 730 740 750
EALEKLNKPS QLEDSEDSLY SDYVDVFYNT KPYKHRDDRL LQALMDILNE

KN
Length:752
Mass (Da):84,121
Last modified:December 1, 1992 - v1
Checksum:iF630AD830A076DED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti366T → TT in AAA41476 (PubMed:1954888).Curated1
Sequence conflicti514E → A in AAA41476 (PubMed:1954888).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76705 mRNA Translation: AAA40945.1
M83745 mRNA Translation: AAA41476.1
PIRiA41556 KXRTC1
RefSeqiNP_058787.1, NM_017091.2
UniGeneiRn.11384

Genome annotation databases

EnsembliENSRNOT00000015185; ENSRNOP00000015185; ENSRNOG00000011107
GeneIDi25204
KEGGirno:25204
UCSCiRGD:3272 rat

Similar proteinsi

Entry informationi

Entry nameiNEC1_RAT
AccessioniPrimary (citable) accession number: P28840
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 25, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome