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P28840

- NEC1_RAT

UniProt

P28840 - NEC1_RAT

Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

    Catalytic activityi

    Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

    Cofactori

    Calcium.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei167 – 1671Charge relay systemBy similarity
    Active sitei208 – 2081Charge relay systemBy similarity
    Active sitei382 – 3821Charge relay systemBy similarity

    GO - Molecular functioni

    1. chaperone binding Source: RGD
    2. endopeptidase activity Source: RGD
    3. insulin binding Source: RGD
    4. protein complex binding Source: RGD
    5. serine-type endopeptidase activity Source: RefGenome

    GO - Biological processi

    1. neurogenesis Source: RGD
    2. pancreas development Source: RGD
    3. peptide hormone processing Source: RGD
    4. pituitary gland development Source: RGD
    5. positive regulation of protein secretion Source: RGD
    6. protein autoprocessing Source: RGD
    7. proteolysis Source: RGD
    8. response to axon injury Source: RGD
    9. response to calcium ion Source: RGD
    10. response to chlorate Source: RGD
    11. response to drug Source: RGD
    12. response to fatty acid Source: RGD
    13. response to glucocorticoid Source: RGD
    14. response to glucose Source: RGD
    15. response to inorganic substance Source: RGD
    16. response to interleukin-1 Source: RGD
    17. response to lipopolysaccharide Source: RGD
    18. response to morphine Source: RGD
    19. response to nutrient levels Source: RGD
    20. response to organic cyclic compound Source: RGD
    21. response to peptide hormone Source: RGD

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Ligandi

    Calcium

    Protein family/group databases

    MEROPSiS08.072.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine convertase 1 (EC:3.4.21.93)
    Short name:
    NEC 1
    Alternative name(s):
    Prohormone convertase 1
    Proprotein convertase 1
    Short name:
    PC1
    Gene namesi
    Name:Pcsk1
    Synonyms:Bdp, Nec-1, Nec1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi3272. Pcsk1.

    Subcellular locationi

    Cytoplasmic vesiclesecretory vesicle
    Note: Localized in the secretion granules.

    GO - Cellular componenti

    1. axon terminus Source: RGD
    2. dendrite Source: RGD
    3. extracellular space Source: RGD
    4. neuronal cell body Source: RGD
    5. neuron projection Source: RGD
    6. perikaryon Source: RGD
    7. perinuclear region of cytoplasm Source: RGD
    8. rough endoplasmic reticulum Source: RGD
    9. secretory granule Source: RGD
    10. trans-Golgi network Source: RGD
    11. transport vesicle Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasmic vesicle

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 11083Sequence AnalysisPRO_0000027063Add
    BLAST
    Chaini111 – 752642Neuroendocrine convertase 1PRO_0000027064Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi225 ↔ 374By similarity
    Disulfide bondi317 ↔ 347By similarity
    Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi467 ↔ 494By similarity
    Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP28840.
    PRIDEiP28840.

    Expressioni

    Gene expression databases

    GenevestigatoriP28840.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000015185.

    Structurei

    3D structure databases

    ProteinModelPortaliP28840.
    SMRiP28840. Positions 31-103, 122-597.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini162 – 451290Peptidase S8Add
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S8 family. Furin subfamily.Curated
    Contains 1 peptidase S8 domain.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4935.
    GeneTreeiENSGT00750000117689.
    HOGENOMiHOG000192536.
    HOVERGENiHBG008705.
    InParanoidiP28840.
    KOiK01359.
    OMAiNNPGWKK.
    OrthoDBiEOG7BW0JD.
    PhylomeDBiP28840.
    TreeFamiTF314277.

    Family and domain databases

    Gene3Di2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProiIPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view]
    PANTHERiPTHR10795. PTHR10795. 1 hit.
    PfamiPF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view]
    PRINTSiPR00723. SUBTILISIN.
    SUPFAMiSSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28840-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA    50
    EELGYDLLGQ IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE 100
    QQYEKERRKR SVPRDSALNL FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP 150
    VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY 200
    DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE 250
    ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 300
    SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST 350
    LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP 400
    NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV 450
    DLADPRTWRN VPEKKECIIK DNNFEPRALK ANGEVIVEIP TRACEGQENA 500
    INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN 550
    WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH 600
    MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS 650
    VEDRRDEQVQ GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY 700
    EALEKLNKPS QLEDSEDSLY SDYVDVFYNT KPYKHRDDRL LQALMDILNE 750
    KN 752
    Length:752
    Mass (Da):84,121
    Last modified:December 1, 1992 - v1
    Checksum:iF630AD830A076DED
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti366 – 3661T → TT in AAA41476. (PubMed:1954888)Curated
    Sequence conflicti514 – 5141E → A in AAA41476. (PubMed:1954888)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76705 mRNA. Translation: AAA40945.1.
    M83745 mRNA. Translation: AAA41476.1.
    PIRiA41556. KXRTC1.
    RefSeqiNP_058787.1. NM_017091.2.
    XP_003749285.1. XM_003749237.2.
    UniGeneiRn.11384.

    Genome annotation databases

    EnsembliENSRNOT00000015185; ENSRNOP00000015185; ENSRNOG00000011107.
    GeneIDi100911216.
    25204.
    KEGGirno:100911216.
    rno:25204.
    UCSCiRGD:3272. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M76705 mRNA. Translation: AAA40945.1 .
    M83745 mRNA. Translation: AAA41476.1 .
    PIRi A41556. KXRTC1.
    RefSeqi NP_058787.1. NM_017091.2.
    XP_003749285.1. XM_003749237.2.
    UniGenei Rn.11384.

    3D structure databases

    ProteinModelPortali P28840.
    SMRi P28840. Positions 31-103, 122-597.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000015185.

    Protein family/group databases

    MEROPSi S08.072.

    Proteomic databases

    PaxDbi P28840.
    PRIDEi P28840.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000015185 ; ENSRNOP00000015185 ; ENSRNOG00000011107 .
    GeneIDi 100911216.
    25204.
    KEGGi rno:100911216.
    rno:25204.
    UCSCi RGD:3272. rat.

    Organism-specific databases

    CTDi 5122.
    RGDi 3272. Pcsk1.

    Phylogenomic databases

    eggNOGi COG4935.
    GeneTreei ENSGT00750000117689.
    HOGENOMi HOG000192536.
    HOVERGENi HBG008705.
    InParanoidi P28840.
    KOi K01359.
    OMAi NNPGWKK.
    OrthoDBi EOG7BW0JD.
    PhylomeDBi P28840.
    TreeFami TF314277.

    Miscellaneous databases

    NextBioi 605733.
    PROi P28840.

    Gene expression databases

    Genevestigatori P28840.

    Family and domain databases

    Gene3Di 2.60.120.260. 1 hit.
    3.40.50.200. 1 hit.
    InterProi IPR008979. Galactose-bd-like.
    IPR000209. Peptidase_S8/S53_dom.
    IPR023827. Peptidase_S8_Asp-AS.
    IPR022398. Peptidase_S8_His-AS.
    IPR023828. Peptidase_S8_Ser-AS.
    IPR015500. Peptidase_S8_subtilisin-rel.
    IPR022005. Proho_convert.
    IPR009020. Prot_inh_propept.
    IPR002884. PrprotnconvertsP.
    [Graphical view ]
    PANTHERi PTHR10795. PTHR10795. 1 hit.
    Pfami PF01483. P_proprotein. 1 hit.
    PF00082. Peptidase_S8. 1 hit.
    PF12177. Proho_convert. 1 hit.
    [Graphical view ]
    PRINTSi PR00723. SUBTILISIN.
    SUPFAMi SSF49785. SSF49785. 1 hit.
    SSF52743. SSF52743. 1 hit.
    SSF54897. SSF54897. 1 hit.
    PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
    PS00137. SUBTILASE_HIS. 1 hit.
    PS00138. SUBTILASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
      Bloomquist B.T., Eipper B.A., Mains R.E.
      Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
      Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
      Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiNEC1_RAT
    AccessioniPrimary (citable) accession number: P28840
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3