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P28840

- NEC1_RAT

UniProt

P28840 - NEC1_RAT

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Protein

Neuroendocrine convertase 1

Gene

Pcsk1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

Catalytic activityi

Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671Charge relay systemBy similarity
Active sitei208 – 2081Charge relay systemBy similarity
Active sitei382 – 3821Charge relay systemBy similarity

GO - Molecular functioni

  1. chaperone binding Source: RGD
  2. endopeptidase activity Source: RGD
  3. insulin binding Source: RGD
  4. protein complex binding Source: RGD
  5. serine-type endopeptidase activity Source: RefGenome

GO - Biological processi

  1. neurogenesis Source: RGD
  2. pancreas development Source: RGD
  3. peptide hormone processing Source: RGD
  4. pituitary gland development Source: RGD
  5. positive regulation of protein secretion Source: RGD
  6. protein autoprocessing Source: RGD
  7. proteolysis Source: RGD
  8. response to axon injury Source: RGD
  9. response to calcium ion Source: RGD
  10. response to chlorate Source: RGD
  11. response to drug Source: RGD
  12. response to fatty acid Source: RGD
  13. response to glucocorticoid Source: RGD
  14. response to glucose Source: RGD
  15. response to inorganic substance Source: RGD
  16. response to interleukin-1 Source: RGD
  17. response to lipopolysaccharide Source: RGD
  18. response to morphine Source: RGD
  19. response to nutrient levels Source: RGD
  20. response to organic cyclic compound Source: RGD
  21. response to peptide hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_229884. Insulin processing.
REACT_233679. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_237271. Peptide hormone biosynthesis.
REACT_246486. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
REACT_250291. Synthesis, secretion, and deacylation of Ghrelin.

Protein family/group databases

MEROPSiS08.072.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 1 (EC:3.4.21.93)
Short name:
NEC 1
Alternative name(s):
Prohormone convertase 1
Proprotein convertase 1
Short name:
PC1
Gene namesi
Name:Pcsk1
Synonyms:Bdp, Nec-1, Nec1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3272. Pcsk1.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle
Note: Localized in the secretion granules.

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. dendrite Source: RGD
  3. extracellular space Source: RGD
  4. neuronal cell body Source: RGD
  5. neuron projection Source: RGD
  6. perikaryon Source: RGD
  7. perinuclear region of cytoplasm Source: RGD
  8. rough endoplasmic reticulum Source: RGD
  9. secretory granule Source: RGD
  10. trans-Golgi network Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 11083Sequence AnalysisPRO_0000027063Add
BLAST
Chaini111 – 752642Neuroendocrine convertase 1PRO_0000027064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi173 – 1731N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi225 ↔ 374By similarity
Disulfide bondi317 ↔ 347By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi467 ↔ 494By similarity
Glycosylationi645 – 6451N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28840.
PRIDEiP28840.

Expressioni

Gene expression databases

ExpressionAtlasiP28840. baseline.
GenevestigatoriP28840.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015185.

Structurei

3D structure databases

ProteinModelPortaliP28840.
SMRiP28840. Positions 31-103, 122-597.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini162 – 451290Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family. Furin subfamily.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
GeneTreeiENSGT00750000117358.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28840.
KOiK01359.
OMAiNNPGWKK.
OrthoDBiEOG7BW0JD.
PhylomeDBiP28840.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28840-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA
60 70 80 90 100
EELGYDLLGQ IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE
110 120 130 140 150
QQYEKERRKR SVPRDSALNL FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP
160 170 180 190 200
VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY
210 220 230 240 250
DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE
260 270 280 290 300
ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG
310 320 330 340 350
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST
360 370 380 390 400
LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP
410 420 430 440 450
NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV
460 470 480 490 500
DLADPRTWRN VPEKKECIIK DNNFEPRALK ANGEVIVEIP TRACEGQENA
510 520 530 540 550
INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN
560 570 580 590 600
WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH
610 620 630 640 650
MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS
660 670 680 690 700
VEDRRDEQVQ GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY
710 720 730 740 750
EALEKLNKPS QLEDSEDSLY SDYVDVFYNT KPYKHRDDRL LQALMDILNE

KN
Length:752
Mass (Da):84,121
Last modified:December 1, 1992 - v1
Checksum:iF630AD830A076DED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti366 – 3661T → TT in AAA41476. (PubMed:1954888)Curated
Sequence conflicti514 – 5141E → A in AAA41476. (PubMed:1954888)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76705 mRNA. Translation: AAA40945.1.
M83745 mRNA. Translation: AAA41476.1.
PIRiA41556. KXRTC1.
RefSeqiNP_058787.1. NM_017091.2.
UniGeneiRn.11384.

Genome annotation databases

EnsembliENSRNOT00000015185; ENSRNOP00000015185; ENSRNOG00000011107.
GeneIDi25204.
KEGGirno:100911216.
rno:25204.
UCSCiRGD:3272. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M76705 mRNA. Translation: AAA40945.1 .
M83745 mRNA. Translation: AAA41476.1 .
PIRi A41556. KXRTC1.
RefSeqi NP_058787.1. NM_017091.2.
UniGenei Rn.11384.

3D structure databases

ProteinModelPortali P28840.
SMRi P28840. Positions 31-103, 122-597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015185.

Protein family/group databases

MEROPSi S08.072.

Proteomic databases

PaxDbi P28840.
PRIDEi P28840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015185 ; ENSRNOP00000015185 ; ENSRNOG00000011107 .
GeneIDi 25204.
KEGGi rno:100911216.
rno:25204.
UCSCi RGD:3272. rat.

Organism-specific databases

CTDi 5122.
RGDi 3272. Pcsk1.

Phylogenomic databases

eggNOGi COG4935.
GeneTreei ENSGT00750000117358.
HOGENOMi HOG000192536.
HOVERGENi HBG008705.
InParanoidi P28840.
KOi K01359.
OMAi NNPGWKK.
OrthoDBi EOG7BW0JD.
PhylomeDBi P28840.
TreeFami TF314277.

Enzyme and pathway databases

Reactomei REACT_229884. Insulin processing.
REACT_233679. Synthesis, secretion, and inactivation of Glucose-dependent Insulinotropic Polypeptide (GIP).
REACT_237271. Peptide hormone biosynthesis.
REACT_246486. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
REACT_250291. Synthesis, secretion, and deacylation of Ghrelin.

Miscellaneous databases

NextBioi 605733.
PROi P28840.

Gene expression databases

ExpressionAtlasi P28840. baseline.
Genevestigatori P28840.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view ]
PANTHERi PTHR10795. PTHR10795. 1 hit.
Pfami PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view ]
PRINTSi PR00723. SUBTILISIN.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
    Bloomquist B.T., Eipper B.A., Mains R.E.
    Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
    Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
    Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNEC1_RAT
AccessioniPrimary (citable) accession number: P28840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 26, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3