Reviewed, UniProtKB/Swiss-Prot P28840 (NEC1_RAT)

Names and origin

Protein names

Recommended name:
    Neuroendocrine convertase 1
      Short name=NEC 1
    EC=3.4.21.93
Alternative name(s):
    Prohormone convertase 1
    Proprotein convertase 1
      Short name=PC1

Gene names

Name:	Pcsk1
Synonyms:	Bdp, Nec-1, Nec1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	752 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.
Catalytic activity	Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.
Cofactor	Calcium.
Subcellular location	Cytoplasmic vesicle › secretory vesicle. Note: Localized in the secretion granules.
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Cytoplasmic vesicle
Domain	Signal
Ligand	Calcium
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	peptide hormone processing Inferred from mutant phenotype. Source: RGD proteolysis Inferred from mutant phenotype. Source: RGD
Cellular component	secretory granule Inferred from direct assay. Source: RGD
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW chaperone binding Inferred from physical interaction. Source: RGD insulin binding Inferred from physical interaction. Source: RGD serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Propeptide	28 – 110	83	Potential		PRO_0000027063
	Chain	111 – 752	642	Neuroendocrine convertase 1		PRO_0000027064
Regions
	Region	122 – 410	289	Catalytic
	Region	739 – 751	13	Amphipathic Potential
Sites
	Active site	167	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
	Active site	382	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	173	1	N-linked (GlcNAc...) Potential
	Glycosylation	401	1	N-linked (GlcNAc...) Potential
	Glycosylation	645	1	N-linked (GlcNAc...) Potential
	Disulfide bond	225 ↔ 374		By similarity
	Disulfide bond	317 ↔ 347		By similarity
	Disulfide bond	467 ↔ 494		By similarity
Experimental info
	Sequence conflict	366	1	T → TT in AAA41476. Ref.2
	Sequence conflict	514	1	E → A in AAA41476. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28840-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: F630AD830A076DED	FASTA	752	84,121
	10 20 30 40 50 60 MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA EELGYDLLGQ 70 80 90 100 110 120 IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE QQYEKERRKR SVPRDSALNL 130 140 150 160 170 180 FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP VWQKGITGKG VVITVLDDGL EWNHTDIYAN 190 200 210 220 230 240 YDPEASYDFN DNDHDPFPRY DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM 250 260 270 280 290 300 LDGIVTDAIE ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 310 320 330 340 350 360 SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST LATSYSSGDY 370 380 390 400 410 420 TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP NLTWRDMQHL VVWTSEYDPL 430 440 450 460 470 480 ANNPGWKKNG AGLMVNSRFG FGLLNAKALV DLADPRTWRN VPEKKECIIK DNNFEPRALK 490 500 510 520 530 540 ANGEVIVEIP TRACEGQENA INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE 550 560 570 580 590 600 RDTSPNGFKN WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH 610 620 630 640 650 660 MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS VEDRRDEQVQ 670 680 690 700 710 720 GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY EALEKLNKPS QLEDSEDSLY 730 740 750 SDYVDVFYNT KPYKHRDDRL LQALMDILNE KN

References

[1]	"Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA." Bloomquist B.T., Eipper B.A., Mains R.E. Mol. Endocrinol. 5:2014-2024(1991) [PubMed: 1791845] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats." Hakes D.J., Birch N.P., Mezey A., Dixon J.E. Endocrinology 129:3053-3063(1991) [PubMed: 1954888] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M76705 mRNA. Translation: AAA40945.1. M83745 mRNA. Translation: AAA41476.1.
IPI	IPI00204862.
PIR	KXRTC1. A41556.
RefSeq	NP_058787.1.
UniGene	Rn.11384
3D structure databases
HSSP	HSSP built from PDB template 1KN6 based on UniProtKB P21662.
ModBase	Search...
Protein family/group databases
MEROPS	S08.072.
Proteomic databases
PRIDE	P28840.
Genome annotation databases
Ensembl	ENSRNOG00000011107. Rattus norvegicus. [Contig view]
GeneID	25204.
KEGG	rno:25204.
Organism-specific databases
RGD	3272. Pcsk1.
Phylogenomic databases
HOVERGEN	P28840.
OMA	P28840. HDPFPRY.
Enzyme and pathway databases
BRENDA	3.4.21.93. 248.
Gene expression databases
ArrayExpress	P28840.
GermOnline	ENSRNOG00000011107. Rattus norvegicus.
Family and domain databases
InterPro	IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
ProDom	PD000717. PrprotnconvertsP. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	605733.

Entry information

Entry name

NEC1_RAT

Accession

Primary (citable) accession number: P28840

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)