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P28840

- NEC1_RAT

UniProt

P28840 - NEC1_RAT

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Protein

Neuroendocrine convertase 1

Gene
Pcsk1, Bdp, Nec-1, Nec1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

Catalytic activityi

Release of protein hormones, neuropeptides and renin from their precursors, generally by hydrolysis of -Lys-Arg-|- bonds.

Cofactori

Calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei167 – 1671Charge relay system By similarity
Active sitei208 – 2081Charge relay system By similarity
Active sitei382 – 3821Charge relay system By similarity

GO - Molecular functioni

  1. chaperone binding Source: RGD
  2. endopeptidase activity Source: RGD
  3. insulin binding Source: RGD
  4. protein complex binding Source: RGD
  5. serine-type endopeptidase activity Source: RefGenome

GO - Biological processi

  1. neurogenesis Source: RGD
  2. pancreas development Source: RGD
  3. peptide hormone processing Source: RGD
  4. pituitary gland development Source: RGD
  5. positive regulation of protein secretion Source: RGD
  6. protein autoprocessing Source: RGD
  7. proteolysis Source: RGD
  8. response to axon injury Source: RGD
  9. response to calcium ion Source: RGD
  10. response to chlorate Source: RGD
  11. response to drug Source: RGD
  12. response to fatty acid Source: RGD
  13. response to glucocorticoid Source: RGD
  14. response to glucose Source: RGD
  15. response to inorganic substance Source: RGD
  16. response to interleukin-1 Source: RGD
  17. response to lipopolysaccharide Source: RGD
  18. response to morphine Source: RGD
  19. response to nutrient levels Source: RGD
  20. response to organic cyclic compound Source: RGD
  21. response to peptide hormone Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiS08.072.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine convertase 1 (EC:3.4.21.93)
Short name:
NEC 1
Alternative name(s):
Prohormone convertase 1
Proprotein convertase 1
Short name:
PC1
Gene namesi
Name:Pcsk1
Synonyms:Bdp, Nec-1, Nec1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi3272. Pcsk1.

Subcellular locationi

Cytoplasmic vesiclesecretory vesicle
Note: Localized in the secretion granules.

GO - Cellular componenti

  1. axon terminus Source: RGD
  2. dendrite Source: RGD
  3. extracellular space Source: RGD
  4. neuronal cell body Source: RGD
  5. neuron projection Source: RGD
  6. perikaryon Source: RGD
  7. perinuclear region of cytoplasm Source: RGD
  8. rough endoplasmic reticulum Source: RGD
  9. secretory granule Source: RGD
  10. trans-Golgi network Source: RGD
  11. transport vesicle Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Propeptidei28 – 11083 Reviewed predictionPRO_0000027063Add
BLAST
Chaini111 – 752642Neuroendocrine convertase 1PRO_0000027064Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi173 – 1731N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi225 ↔ 374 By similarity
Disulfide bondi317 ↔ 347 By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi467 ↔ 494 By similarity
Glycosylationi645 – 6451N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP28840.
PRIDEiP28840.

Expressioni

Gene expression databases

GenevestigatoriP28840.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015185.

Structurei

3D structure databases

ProteinModelPortaliP28840.
SMRiP28840. Positions 31-103, 122-597.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni122 – 410289CatalyticAdd
BLAST
Regioni739 – 75113Amphipathic Reviewed predictionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG4935.
GeneTreeiENSGT00750000117689.
HOGENOMiHOG000192536.
HOVERGENiHBG008705.
InParanoidiP28840.
KOiK01359.
OMAiNNPGWKK.
OrthoDBiEOG7BW0JD.
PhylomeDBiP28840.
TreeFamiTF314277.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28840-1 [UniParc]FASTAAdd to Basket

« Hide

MKQRGWTLQC TAFTLFCVWC ALNSVKAKRQ FVNEWAAEIH GGPEAASAIA    50
EELGYDLLGQ IGSLENHYLF KHKNHPRRSR RSALHITKRL SDDDRVIWAE 100
QQYEKERRKR SVPRDSALNL FNDPMWNQQW YLQDTRMTAS LPKLDLHVIP 150
VWQKGITGKG VVITVLDDGL EWNHTDIYAN YDPEASYDFN DNDHDPFPRY 200
DPTNENKHGT RCAGEIAMQA NNHKCGVGVA YNSKVGGIRM LDGIVTDAIE 250
ASSIGFNPGH VDIYSASWGP NDDGKTVEGP GRLAQKAFEY GVKQGRQGKG 300
SIFVWASGNG GRQGDNCDCD GYTDSIYTIS ISSASQQGLS PWYAEKCSST 350
LATSYSSGDY TDQRITSADL HNDCTETHTG TSASAPLAAG IFALALEANP 400
NLTWRDMQHL VVWTSEYDPL ANNPGWKKNG AGLMVNSRFG FGLLNAKALV 450
DLADPRTWRN VPEKKECIIK DNNFEPRALK ANGEVIVEIP TRACEGQENA 500
INSLEHVQFE ATIEYSRRGD LHVTLTSAAG TSTVLLAERE RDTSPNGFKN 550
WDFMSVHTWG ENPVGTWTLK VTDMSGRMQN EGRIVNWKLI LHGTSSQPEH 600
MKQPRVYTSY NTVQNDRRGV EKMVNVVEEK PTQNSLNGNL LVPKNSSSSS 650
VEDRRDEQVQ GAPSKAMLRL LQSAFSKNTP SKQSSKIPSA KLSVPYEGLY 700
EALEKLNKPS QLEDSEDSLY SDYVDVFYNT KPYKHRDDRL LQALMDILNE 750
KN 752
Length:752
Mass (Da):84,121
Last modified:December 1, 1992 - v1
Checksum:iF630AD830A076DED
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti366 – 3661T → TT in AAA41476. 1 Publication
Sequence conflicti514 – 5141E → A in AAA41476. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76705 mRNA. Translation: AAA40945.1.
M83745 mRNA. Translation: AAA41476.1.
PIRiA41556. KXRTC1.
RefSeqiNP_058787.1. NM_017091.2.
XP_003749285.1. XM_003749237.2.
UniGeneiRn.11384.

Genome annotation databases

EnsembliENSRNOT00000015185; ENSRNOP00000015185; ENSRNOG00000011107.
GeneIDi100911216.
25204.
KEGGirno:100911216.
rno:25204.
UCSCiRGD:3272. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M76705 mRNA. Translation: AAA40945.1 .
M83745 mRNA. Translation: AAA41476.1 .
PIRi A41556. KXRTC1.
RefSeqi NP_058787.1. NM_017091.2.
XP_003749285.1. XM_003749237.2.
UniGenei Rn.11384.

3D structure databases

ProteinModelPortali P28840.
SMRi P28840. Positions 31-103, 122-597.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000015185.

Protein family/group databases

MEROPSi S08.072.

Proteomic databases

PaxDbi P28840.
PRIDEi P28840.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000015185 ; ENSRNOP00000015185 ; ENSRNOG00000011107 .
GeneIDi 100911216.
25204.
KEGGi rno:100911216.
rno:25204.
UCSCi RGD:3272. rat.

Organism-specific databases

CTDi 5122.
RGDi 3272. Pcsk1.

Phylogenomic databases

eggNOGi COG4935.
GeneTreei ENSGT00750000117689.
HOGENOMi HOG000192536.
HOVERGENi HBG008705.
InParanoidi P28840.
KOi K01359.
OMAi NNPGWKK.
OrthoDBi EOG7BW0JD.
PhylomeDBi P28840.
TreeFami TF314277.

Miscellaneous databases

NextBioi 605733.
PROi P28840.

Gene expression databases

Genevestigatori P28840.

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
3.40.50.200. 1 hit.
InterProi IPR008979. Galactose-bd-like.
IPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR022005. Proho_convert.
IPR009020. Prot_inh_propept.
IPR002884. PrprotnconvertsP.
[Graphical view ]
PANTHERi PTHR10795. PTHR10795. 1 hit.
Pfami PF01483. P_proprotein. 1 hit.
PF00082. Peptidase_S8. 1 hit.
PF12177. Proho_convert. 1 hit.
[Graphical view ]
PRINTSi PR00723. SUBTILISIN.
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEi PS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Prohormone-converting enzymes: regulation and evaluation of function using antisense RNA."
    Bloomquist B.T., Eipper B.A., Mains R.E.
    Mol. Endocrinol. 5:2014-2024(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation of two complementary deoxyribonucleic acid clones from a rat insulinoma cell line based on similarities to Kex2 and furin sequences and the specific localization of each transcript to endocrine and neuroendocrine tissues in rats."
    Hakes D.J., Birch N.P., Mezey A., Dixon J.E.
    Endocrinology 129:3053-3063(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiNEC1_RAT
AccessioniPrimary (citable) accession number: P28840
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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