Protein kinase byr2 - Schizosaccharomyces pombe (Fission yeast)

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Reviewed, UniProtKB/Swiss-Prot P28829 (BYR2_SCHPO)

Last modified January 19, 2010. Version 99. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Protein kinase byr2
    EC=2.7.11.25
Alternative name(s):
    Protein kinase ste8
    MAPK kinase kinase
      Short name=MAPKKK

Gene names

Name:	byr2
Synonyms:	ste8
ORF Names:	SPBC1D7.05, SPBC2F12.01

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

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Protein attributes

Sequence length	659 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function

Serine/threonine protein kinase involved in conjugation and sporulation. It is thought that it phosphorylates the byr1 protein kinase which itself phosphorylate the spk1 kinase.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

Contains 1 protein kinase domain.

Contains 1 SAM (sterile alpha motif) domain.

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Ontologies

Keywords
Biological process	Conjugation Sporulation
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell cycle arrest in response to pheromone Traceable author statement. Source: GeneDB_SPombe induction of conjugation upon nitrogen starvation Traceable author statement. Source: GeneDB_SPombe protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro signal transduction involved in conjugation with cellular fusion Traceable author statement. Source: GeneDB_SPombe sporulation resulting in formation of a cellular spore Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: GeneDB_SPombe plasma membrane Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Traceable author statement. Source: GeneDB_SPombe protein binding Ref.5 Inferred from physical interaction. Source: IntAct
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
HRAS	P01112	1	EBI-1032333,EBI-350145	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 659

659

Protein kinase byr2

PRO_0000085689

Regions

Domain

4 – 67

SAM

Domain

394 – 658

265

Protein kinase

Nucleotide binding

400 – 408

ATP By similarity

Sites

Active site

522

Proton acceptor By similarity

Binding site

423

ATP By similarity

Secondary structure

659

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P28829-1 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 18CD78F1D1FABBCD
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FASTA

659

73,633

        10         20         30         40         50         60 
MEYYTSKEVA EWLKSIGLEK YIEQFSQNNI EGRHLNHLTL PLLKDLGIEN TAKGKQFLKQ 

        70         80         90        100        110        120 
RDYLREFPRP CILRFIACNG QTRAVQSRGD YQKTLAIALK KFSLEDASKF IVCVSQSSRI 

       130        140        150        160        170        180 
KLITEEEFKQ ICFNSSSPER DRLIIVPKEK PCPSFEDLRR SWEIELAQPA ALSSQSSLSP 

       190        200        210        220        230        240 
KLSSVLPTST QKRSVRSNNA KPFESYQRPP SELINSRISD FFPDHQPKLL EKTISNSLRR 

       250        260        270        280        290        300 
NLSIRTSQGH NLGNFGQEIL PRSSRRARPS ELVCPLSSLR ISVAEDVNRL PRIDRGFDPP 

       310        320        330        340        350        360 
LTVSSTQRIS RPPSLQKSIT MVGVEPLYQS NGNEKSSKYN VFSESAHGNH QVLSFSPGSS 

       370        380        390        400        410        420 
PSFIEQPSPI SPTSTTSEDT NTLEEDTDDQ SIKWIRGALI GSGSFGQVYL GMNASSGELM 

       430        440        450        460        470        480 
AVKQVILDSV SESKDRHAKL LDALAGEIAL LQELSHEHIV QYLGSNLNSD HLNIFLEYVP 

       490        500        510        520        530        540 
GGSVAGLLTM YGSFEETLVK NFIKQTLKGL EYLHSRGIVH RDIKGANILV DNKGKIKISD 

       550        560        570        580        590        600 
FGISKKLELN STSTKTGGAR PSFQGSSFWM APEVVKQTMH TEKTDIWSLG CLVIEMLTSK 

       610        620        630        640        650 
HPYPNCDQMQ AIFRIGENIL PEFPSNISSS AIDFLEKTFA IDCNLRPTAS ELLSHPFVS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"byr2, a Schizosaccharomyces pombe gene encoding a protein kinase capable of partial suppression of the ras1 mutant phenotype." Wang Y., Xu H.P., Riggs M., Rodgers L., Wigler M. Mol. Cell. Biol. 11:3554-3563(1991) [PubMed: 2046669] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Functional conservation between Schizosaccharomyces pombe ste8 and Saccharomyces cerevisiae STE11 protein kinases in yeast signal transduction." Styrkarsdottir U., Egel R., Nielsen O. Mol. Gen. Genet. 235:122-130(1992) [PubMed: 1435723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[4]	"Overcoming the problems associated with poor spectra quality of the protein kinase Byr2 using residual dipolar couplings." Gronwald W., Brunner E., Huber F., Wenzler M., Herrmann C., Kalbitzer H.R. Protein Sci. 10:1260-1263(2001) [PubMed: 11369865] [Abstract] Cited for: STRUCTURE BY NMR OF 71-165.
[5]	"The Ras-Byr2RBD complex: structural basis for Ras effector recognition in yeast." Scheffzek K., Grunewald P., Wohlgemuth S., Kabsch W., Tu H., Wigler M., Wittinghofer A., Herrmann C. Structure 9:1043-1050(2001) [PubMed: 11709168] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS) OF 81-180.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M74293 Genomic DNA. Translation: AAA35289.1.
X68851 Genomic DNA. Translation: CAA48731.1.
CU329671 Genomic DNA. Translation: CAB10150.1.

PIR

A39723.

RefSeq

NP_595714.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1I35	NMR	-	A	71-165	[»]
1K8R	X-ray	3.00	B	71-180	[»]

SMR

P28829. Positions 2-67, 393-658.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-136N.

IntAct

P28829. 1 interaction.

STRING

P28829.

Genome annotation databases

GeneID

2540612.

GenomeReviews

Gene locus byr2 in contig CU329671_GR.

KEGG

spo:SPBC1D7.05.

Organism-specific databases

GeneDB_Spombe

SPBC1D7.05.

Phylogenomic databases

OMA

RIGENIL.

OrthoDB

EOG915HWN.

PhylomeDB

P28829.

Enzyme and pathway databases

BRENDA

2.7.11.25. 653.

Gene expression databases

ArrayExpress

P28829.

Family and domain databases

InterPro

IPR011009. Kinase-like_dom.
IPR015748. MAPKKK3.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR011510. SAM_2.
IPR010993. SAM_homology.
IPR013761. SAM_type.
IPR017442. Se/Thr_prot_kinase-like_dom.
IPR008271. Ser/Thr_prot_kinase_AS.
IPR002290. Ser/Thr_prot_kinase_dom.
[Graphical view]

Gene3D

G3DSA:1.10.150.50. SAM_type. 1 hit.

PANTHER

PTHR22986:SF64. MAPKKK3. 1 hit.

Pfam

PF00069. Pkinase. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]

SMART

SM00220. S_TKc. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]

PROSITE

PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

BYR2_SCHPO

Accession

Primary (citable) accession number: P28829
Secondary accession number(s): Q7LGJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	January 19, 2010
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents