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Reviewed, UniProtKB/Swiss-Prot P28825 (MEP1A_MOUSE)

Last modified June 16, 2009. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Meprin A subunit alpha
    EC=3.4.24.18
Alternative name(s):
    Endopeptidase-2
    MEP-1
Gene names
Name: Mep1a
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length747 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

Hydrolysis of protein and peptide substrates preferentially on carboxyl side of hydrophobic residues.

Cofactor

Binds 1 zinc ion per subunit By similarity.

Subunit structure

Homotetramer of alpha or beta subunits; heterotetramer of two alpha and two beta subunits are formed by non-covalent association of two disulfide-linked heterodimers; genetic factors determine which oligomer(s) will be formed (strain-specific).

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Kidney, intestinal brush borders and salivary ducts.

Post-translational modification

N-glycosylated; at least 3 of the potential sites are used.

Sequence similarities

Belongs to the peptidase M12A family.

Contains 1 EGF-like domain.

Contains 1 MAM domain.

Contains 1 MATH domain.

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Ontologies

Keywords
   Cellular componentMembrane
   DomainEGF-like domain
Signal
Transmembrane
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentmembrane fraction

Inferred from direct assay. Source: MGI

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Ref.2
Propeptide21 – 6444
PRO_0000028879
Chain65 – 747683Meprin A subunit alpha
PRO_0000028880

Regions

Topological domain65 – 713649Extracellular Potential
Transmembrane714 – 74128 Potential
Topological domain742 – 7476Cytoplasmic Potential
Domain263 – 432170MAM
Domain433 – 594162MATH
Domain671 – 71141EGF-like
Region65 – 262198Metalloprotease

Sites

Active site1551 By similarity
Metal binding1541Zinc; catalytic By similarity
Metal binding1581Zinc; catalytic By similarity
Metal binding1641Zinc; catalytic By similarity

Amino acid modifications

Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation1391N-linked (GlcNAc...) Potential
Glycosylation2211N-linked (GlcNAc...) Potential
Glycosylation2571N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Potential
Glycosylation4131N-linked (GlcNAc...) Potential
Glycosylation4391N-linked (GlcNAc...) Potential
Glycosylation5331N-linked (GlcNAc...) Potential
Glycosylation5401N-linked (GlcNAc...) Potential
Glycosylation6011N-linked (GlcNAc...) Potential
Disulfide bond307Interchain (with C-306 in MEP1B) By similarity
Disulfide bond675 ↔ 686 By similarity
Disulfide bond680 ↔ 695 By similarity
Disulfide bond697 ↔ 710 By similarity

Secondary structure

1 ..................................... 747
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P28825-1 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 2A18242A43505633

FASTA74784,197
        10         20         30         40         50         60 
MLWIQPACLL SLIFSAHIAA VSIKHLLNGS DHDTDVGEQK DIFEINLAAG LNLFQGDILL 

        70         80         90        100        110        120 
PRTRNAMRDP SSRWKLPIPY ILADNLELNA KGAILHAFEM FRLKSCVDFK PYEGESSYII 

       130        140        150        160        170        180 
FQKLSGCWSM IGDQQVGQNI SIGEGCDFKA TIEHEILHAL GFFHEQSRTD RDDYVNIWWD 

       190        200        210        220        230        240 
QIITDYEHNF NTYDDNTITD LNTPYDYESL MHYGPFSFNK NESIPTITTK IPEFNTIIGQ 

       250        260        270        280        290        300 
LPDFSAIDLI RLNRMYNCTA THTLLDHCDF EKTNVCGMIQ GTRDDADWAH GDSSQPEQVD 

       310        320        330        340        350        360 
HTLVGQCKGA GYFMFFNTSL GARGEAALLE SRILYPKRKQ QCLQFFYKMT GSPADRFEVW 

       370        380        390        400        410        420 
VRRDDNAGKV RQLAKIQTFQ GDSDHNWKIA HVTLNEEKKF RYVFLGTKGD PGNSSGGIYL 

       430        440        450        460        470        480 
DDITLTETPC PAGVWTIRNI SQILENTVKG DKLVSPRFYN SEGYGVGVTL YPNGRITSNS 

       490        500        510        520        530        540 
GLLGLTFHLY SGDNDAILEW PVENRQAIMT ILDQEADTRN RMSLTLMFTT SKNQTSSAIN 

       550        560        570        580        590        600 
GSVIWDRPSK VGVYDKDCDC FRSLDWGWGQ AISHQLLKRR NFLKGDSLII FVDFKDLTHL 

       610        620        630        640        650        660 
NRTEVPASAR STMPRGLLLQ GQESPALGES SRKAMLEESL PSSLGQRHPS RQKRSVENTG 

       670        680        690        700        710        720 
PMEDHNWPQY FRDPCDPNPC QNEGTCVNVK GMASCRCVSG HAFFYAGERC QAMHVHGSLL 

       730        740 
GLLIGCIAGL IFLTFVTFST TNGKLRQ

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References

[1]"The alpha subunit of meprin A. Molecular cloning and sequencing, differential expression in inbred mouse strains, and evidence for divergent evolution of the alpha and beta subunits."
Jiang W., Gorbea C.M., Flannery A.V., Beynon R.J., Grant G.A., Bond J.S.
J. Biol. Chem. 267:9185-9193(1992) [PubMed: 1374387] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: C3H/He and C57BL/6.
Tissue: Kidney.
[2]"Characterization of the soluble, secreted form of urinary meprin."
Beynon R.J., Oliver S., Robertson D.H.L.
Biochem. J. 315:461-465(1996) [PubMed: 8615815] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-25 AND 65-69.
[3]"The astacin family of metalloendopeptidases."
Dumermuth E., Sterchi E.E., Jiang W., Wolz R.L., Bond J.S., Flannery A.V., Beynon R.J.
J. Biol. Chem. 266:21381-21385(1991) [PubMed: 1939172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 64-262.
[4]"Correlation of the exon/intron organization to the secondary structures of the protease domain of mouse meprin alpha subunit."
Jiang W., Flannery A.V.
Gene 189:65-71(1997) [PubMed: 9161413] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 62-258.
Strain: 129.
[5]"Mapping the active site of meprin-A with peptide substrates and inhibitors."
Wolz R.L., Harris R.B., Bond J.S.
Biochemistry 30:8488-8493(1991) [PubMed: 1883833] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Implications of the three-dimensional structure of astacin for the structure and function of the astacin family of zinc-endopeptidases."
Stoecker W., Gomis-Rueth F.-X., Bode W., Zwilling R.
Eur. J. Biochem. 214:215-231(1993) [PubMed: 8508794] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M74897 mRNA. Translation: AAA75354.1. Different initiation.
U62765 Genomic DNA. Translation: AAC53194.1.
IPIIPI00314042.
PIRA40195.
UniGeneMm.5346

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1IAFmodel-A65-261[»]
ModBaseSearch...

Protein family/group databases

MEROPSM12.002.

Genome annotation databases

EnsemblENSMUSG00000023914. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:96963. Mep1a.

Phylogenomic databases

HOVERGENP28825.

Enzyme and pathway databases

BRENDA3.4.24.18. 244.

Gene expression databases

ArrayExpressP28825.
BgeeP28825.
CleanExMM_MEP1A.
GermOnlineENSMUSG00000023914. Mus musculus.

Family and domain databases

InterProIPR006209. EGF.
IPR006210. EGF-like.
IPR013032. EGF-like_reg_CS.
IPR000742. EGF_3.
IPR000998. MAM.
IPR002083. MATH.
IPR008294. Pept_M12A_Meprin.
IPR006025. Pept_M_Zn_BS.
IPR006026. Peptidase_M.
IPR001506. Peptidase_M12A.
IPR013322. TRAF-type.
[Graphical view]
Gene3DG3DSA:2.60.210.10. TRAF-type. 1 hit.
PfamPF01400. Astacin. 1 hit.
PF00008. EGF. 1 hit.
PF00629. MAM. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
PIRSFPIRSF001196. Meprin. 1 hit.
PRINTSPR00480. ASTACIN.
PR00020. MAMDOMAIN.
SMARTSM00181. EGF. 1 hit.
SM00137. MAM. 1 hit.
SM00061. MATH. 1 hit.
SM00235. ZnMc. 1 hit.
[Graphical view]
PROSITEPS00022. EGF_1. False negative.
PS01186. EGF_2. False negative.
PS50026. EGF_3. 1 hit.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50144. MATH. 1 hit.
PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry nameMEP1A_MOUSE
AccessionPrimary (citable) accession number: P28825
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: June 16, 2009
This is version 96 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents