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Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

Rasgrf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • Ras GTPase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • activation of GTPase activity Source: RGD
  • cellular response to antibiotic Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • neuron projection development Source: HGNC
  • positive regulation of fibroblast proliferation Source: RGD
  • positive regulation of GTPase activity Source: RGD
  • positive regulation of Ras protein signal transduction Source: UniProtKB
  • regulation of Rac protein signal transduction Source: HGNC
  • regulation of Ras protein signal transduction Source: HGNC
  • regulation of Rho protein signal transduction Source: InterPro
  • regulation of synaptic plasticity Source: UniProtKB
  • response to amphetamine Source: RGD
  • small GTPase mediated signal transduction Source: InterPro

Keywordsi

Molecular functionGuanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
Guanine nucleotide-releasing protein
Short name:
GNRP
P140 Ras-GRF
Gene namesi
Name:Rasgrf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620395 Rasgrf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71S → A: Abolishes degradation by the proteasome. 1 Publication1
Mutagenesisi575S → A: Abolishes degradation by the proteasome. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688821 – 1244Ras-specific guanine nucleotide-releasing factor 1Add BLAST1244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71Phosphoserine; by PLK21 Publication1
Modified residuei575Phosphoserine; by PLK21 Publication1
Modified residuei611Phosphoserine; by PLK21 Publication1
Modified residuei739PhosphoserineBy similarity1
Modified residuei760Phosphoserine; by PLK21 Publication1
Modified residuei781Phosphoserine; by PLK21 Publication1
Modified residuei854Phosphoserine; by PLK21 Publication1

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.1 Publication
Ubiquitinated and degraded following phosphorylation by PLK2.1 Publication
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP28818
PRIDEiP28818

PTM databases

iPTMnetiP28818
PhosphoSitePlusiP28818

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).By similarity

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • Ras GTPase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

Protein-protein interaction databases

BioGridi251354, 1 interactor
STRINGi10116.ENSRNOP00000018794

Structurei

3D structure databases

ProteinModelPortaliP28818
SMRiP28818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 129PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini204 – 229IQPROSITE-ProRule annotationAdd BLAST26
Domaini240 – 426DHPROSITE-ProRule annotationAdd BLAST187
Domaini456 – 582PH 2PROSITE-ProRule annotationAdd BLAST127
Domaini629 – 743N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST115
Domaini1009 – 1241Ras-GEFPROSITE-ProRule annotationAdd BLAST233

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q Eukaryota
ENOG410XPWA LUCA
HOGENOMiHOG000046000
HOVERGENiHBG005208
InParanoidiP28818
KOiK04349
PhylomeDBiP28818

Family and domain databases

CDDicd00155 RasGEF, 1 hit
cd00160 RhoGEF, 1 hit
Gene3Di1.10.840.10, 1 hit
1.20.900.10, 1 hit
2.30.29.30, 2 hits
InterProiView protein in InterPro
IPR035899 DBL_dom_sf
IPR000219 DH-domain
IPR001331 GDS_CDC24_CS
IPR000048 IQ_motif_EF-hand-BS
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000651 Ras-like_Gua-exchang_fac_N
IPR019804 Ras_G-nucl-exch_fac_CS
IPR023578 Ras_GEF_dom_sf
IPR001895 RASGEF_cat_dom
IPR036964 RASGEF_cat_dom_sf
IPR030745 RasGRF1
PANTHERiPTHR23113:SF193 PTHR23113:SF193, 1 hit
PfamiView protein in Pfam
PF00169 PH, 2 hits
PF00617 RasGEF, 1 hit
PF00618 RasGEF_N, 1 hit
PF00621 RhoGEF, 1 hit
SMARTiView protein in SMART
SM00233 PH, 2 hits
SM00147 RasGEF, 1 hit
SM00229 RasGEFN, 2 hits
SM00325 RhoGEF, 1 hit
SUPFAMiSSF48065 SSF48065, 1 hit
SSF48366 SSF48366, 2 hits
PROSITEiView protein in PROSITE
PS00741 DH_1, 1 hit
PS50010 DH_2, 1 hit
PS50096 IQ, 1 hit
PS50003 PH_DOMAIN, 2 hits
PS00720 RASGEF, 1 hit
PS50009 RASGEF_CAT, 1 hit
PS50212 RASGEF_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P28818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAIRLNDG HVVSLGLLAQ RDGTRKGYLS KRSSDNPKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG SICKRMPSPK RGTSSKESDK QHHYFTVNFS
110 120 130 140 150
NDSQKSLELR TDDSKDCDEW VAAIARASYK ILATEHEALM QKYLHLLQVV
160 170 180 190 200
ETEKTVAKQL RQQLEDGEVE IERLKAEIAN LIKDNERIQS NQLVAPEDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKNIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR IASWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KQYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RVMHDEVSET ENIRKNLAIE RMITEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKINKGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGSKL HLTKNGVISL IDCTLLDDPE NMDDDGKGQE VDHLDFKIWV
560 570 580 590 600
EPKDSPPFTV ILVASSRQEK AAWTSDIIQC VDNIRCNGLM MNAFEENSKV
610 620 630 640 650
TVPQMIKSDA SLYCDDVDIR FSKTMNSCKV LQIRYASVER LLERLTDLRF
660 670 680 690 700
LSIDFLNTFL HSYRVFTDAV VVLDKLISIY KKPITAIPAR SLELLFSSSH
710 720 730 740 750
NTKLLYGDAP KSPRASRKFS SPPPLAIGTS SPVRRRKLSL NIPIITGGKA
760 770 780 790 800
LELASLGCPS DGYTNIHSPI SPFGKTTLDT SKLCVASSLT RTPEEIDMTT
810 820 830 840 850
LEESSGFRKP TSDILKEESD DDQSDVDDTE VSPPTPKSFR NRITQEFPLF
860 870 880 890 900
NYNSGIMMTC RDLMDSNRSP LSATSAFAIA TAGANESPAN KEIYRRMSLA
910 920 930 940 950
NTGYSSDQRN IDKEFVIRRA ATNRVLNVLR HWVTKHSQDF ETDDLLKYKV
960 970 980 990 1000
ICFLEEVMHD PDLLPQERKA AANIMRTLTQ EEITENHSML DELLLMTEGV
1010 1020 1030 1040 1050
KTEPFENHSA MEIAEQLTLL DHLVFKSIPY EEFFGQGWMK ADKNERTPYI
1060 1070 1080 1090 1100
MKTTRHFNHI SNLIASEILR NEEVSARAST IEKWVAVADI CRCLHNYNAV
1110 1120 1130 1140 1150
LEITSSINRS AIFRLKKTWL KVSKQTKSLF DKLQKLVSSD GRFKNLRETL
1160 1170 1180 1190 1200
RNCDPPCVPY LGMYLTDLAF LEEGTPNYTE DGLVNFSKMR MISHIIREIR
1210 1220 1230 1240
QFQQTTYKIE PQPKVTQYLV DETFVLDDES LYEASLRIEP KLPT
Length:1,244
Mass (Da):142,667
Last modified:December 1, 1992 - v1
Checksum:i4B647879E842AF6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67241 mRNA Translation: CAA47666.1
PIRiS29083
RefSeqiNP_001164002.1, NM_001170531.1
UniGeneiRn.135776
Rn.230019
Rn.230593

Genome annotation databases

GeneIDi192213
KEGGirno:192213
UCSCiRGD:620395 rat

Similar proteinsi

Entry informationi

Entry nameiRGRF1_RAT
AccessioniPrimary (citable) accession number: P28818
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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