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Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

Rasgrf1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB
  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • Ras GTPase binding Source: RGD
  • receptor tyrosine kinase binding Source: RGD
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • activation of GTPase activity Source: HGNC
  • cellular response to antibiotic Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • neuron projection development Source: HGNC
  • positive regulation of fibroblast proliferation Source: RGD
  • positive regulation of GTPase activity Source: HGNC
  • positive regulation of Ras protein signal transduction Source: UniProtKB
  • regulation of Rac protein signal transduction Source: HGNC
  • regulation of Ras protein signal transduction Source: HGNC
  • regulation of Rho protein signal transduction Source: InterPro
  • regulation of synaptic plasticity Source: UniProtKB
  • response to amphetamine Source: RGD
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
Guanine nucleotide-releasing protein
Short name:
GNRP
P140 Ras-GRF
Gene namesi
Name:Rasgrf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620395. Rasgrf1.

Subcellular locationi

GO - Cellular componenti

  • apical dendrite Source: RGD
  • apicolateral plasma membrane Source: RGD
  • basolateral plasma membrane Source: RGD
  • cytoplasm Source: RGD
  • cytosol Source: HGNC
  • growth cone Source: HGNC
  • neuronal cell body Source: RGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi71S → A: Abolishes degradation by the proteasome. 1 Publication1
Mutagenesisi575S → A: Abolishes degradation by the proteasome. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000688821 – 1244Ras-specific guanine nucleotide-releasing factor 1Add BLAST1244

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei71Phosphoserine; by PLK21 Publication1
Modified residuei575Phosphoserine; by PLK21 Publication1
Modified residuei611Phosphoserine; by PLK21 Publication1
Modified residuei739PhosphoserineBy similarity1
Modified residuei760Phosphoserine; by PLK21 Publication1
Modified residuei781Phosphoserine; by PLK21 Publication1
Modified residuei854Phosphoserine; by PLK21 Publication1

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.1 Publication
Ubiquitinated and degraded following phosphorylation by PLK2.1 Publication
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP28818.
PRIDEiP28818.

PTM databases

iPTMnetiP28818.
PhosphoSitePlusiP28818.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability (By similarity).By similarity

GO - Molecular functioni

  • protein C-terminus binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB
  • Ras GTPase binding Source: RGD
  • receptor tyrosine kinase binding Source: RGD

Protein-protein interaction databases

BioGridi251354. 1 interactor.
STRINGi10116.ENSRNOP00000018794.

Structurei

3D structure databases

ProteinModelPortaliP28818.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini22 – 129PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini204 – 229IQPROSITE-ProRule annotationAdd BLAST26
Domaini240 – 426DHPROSITE-ProRule annotationAdd BLAST187
Domaini456 – 582PH 2PROSITE-ProRule annotationAdd BLAST127
Domaini629 – 743N-terminal Ras-GEFPROSITE-ProRule annotationAdd BLAST115
Domaini1009 – 1241Ras-GEFPROSITE-ProRule annotationAdd BLAST233

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.By similarity

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiP28818.
KOiK04349.
PhylomeDBiP28818.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 3 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28818-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAIRLNDG HVVSLGLLAQ RDGTRKGYLS KRSSDNPKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSS RPSGLYLLEG SICKRMPSPK RGTSSKESDK QHHYFTVNFS
110 120 130 140 150
NDSQKSLELR TDDSKDCDEW VAAIARASYK ILATEHEALM QKYLHLLQVV
160 170 180 190 200
ETEKTVAKQL RQQLEDGEVE IERLKAEIAN LIKDNERIQS NQLVAPEDED
210 220 230 240 250
SDIKKIKKVQ SFLRGWLCRR KWKNIIQDYI RSPHADSMRK RNQVVFSMLE
260 270 280 290 300
AEAEYVQQLH ILVNNFLRPL RMAASSKKPP ITHDDVSSIF LNSETIMFLH
310 320 330 340 350
QIFYQGLKAR IASWPTLVLA DLFDILLPML NIYQEFVRNH QYSLQILAHC
360 370 380 390 400
KQNRDFDKLL KQYEAKPDCE ERTLETFLTY PMFQIPRYIL TLHELLAHTP
410 420 430 440 450
HEHVERNSLD YAKSKLEELS RVMHDEVSET ENIRKNLAIE RMITEGCEIL
460 470 480 490 500
LDTSQTFVRQ GSLIQVPMSE KGKINKGRLG SLSLKKEGER QCFLFSKHLI
510 520 530 540 550
ICTRGSGSKL HLTKNGVISL IDCTLLDDPE NMDDDGKGQE VDHLDFKIWV
560 570 580 590 600
EPKDSPPFTV ILVASSRQEK AAWTSDIIQC VDNIRCNGLM MNAFEENSKV
610 620 630 640 650
TVPQMIKSDA SLYCDDVDIR FSKTMNSCKV LQIRYASVER LLERLTDLRF
660 670 680 690 700
LSIDFLNTFL HSYRVFTDAV VVLDKLISIY KKPITAIPAR SLELLFSSSH
710 720 730 740 750
NTKLLYGDAP KSPRASRKFS SPPPLAIGTS SPVRRRKLSL NIPIITGGKA
760 770 780 790 800
LELASLGCPS DGYTNIHSPI SPFGKTTLDT SKLCVASSLT RTPEEIDMTT
810 820 830 840 850
LEESSGFRKP TSDILKEESD DDQSDVDDTE VSPPTPKSFR NRITQEFPLF
860 870 880 890 900
NYNSGIMMTC RDLMDSNRSP LSATSAFAIA TAGANESPAN KEIYRRMSLA
910 920 930 940 950
NTGYSSDQRN IDKEFVIRRA ATNRVLNVLR HWVTKHSQDF ETDDLLKYKV
960 970 980 990 1000
ICFLEEVMHD PDLLPQERKA AANIMRTLTQ EEITENHSML DELLLMTEGV
1010 1020 1030 1040 1050
KTEPFENHSA MEIAEQLTLL DHLVFKSIPY EEFFGQGWMK ADKNERTPYI
1060 1070 1080 1090 1100
MKTTRHFNHI SNLIASEILR NEEVSARAST IEKWVAVADI CRCLHNYNAV
1110 1120 1130 1140 1150
LEITSSINRS AIFRLKKTWL KVSKQTKSLF DKLQKLVSSD GRFKNLRETL
1160 1170 1180 1190 1200
RNCDPPCVPY LGMYLTDLAF LEEGTPNYTE DGLVNFSKMR MISHIIREIR
1210 1220 1230 1240
QFQQTTYKIE PQPKVTQYLV DETFVLDDES LYEASLRIEP KLPT
Length:1,244
Mass (Da):142,667
Last modified:December 1, 1992 - v1
Checksum:i4B647879E842AF6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67241 mRNA. Translation: CAA47666.1.
PIRiS29083.
RefSeqiNP_001164002.1. NM_001170531.1.
UniGeneiRn.135776.
Rn.230019.
Rn.230593.

Genome annotation databases

GeneIDi192213.
KEGGirno:192213.
UCSCiRGD:620395. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X67241 mRNA. Translation: CAA47666.1.
PIRiS29083.
RefSeqiNP_001164002.1. NM_001170531.1.
UniGeneiRn.135776.
Rn.230019.
Rn.230593.

3D structure databases

ProteinModelPortaliP28818.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi251354. 1 interactor.
STRINGi10116.ENSRNOP00000018794.

PTM databases

iPTMnetiP28818.
PhosphoSitePlusiP28818.

Proteomic databases

PaxDbiP28818.
PRIDEiP28818.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192213.
KEGGirno:192213.
UCSCiRGD:620395. rat.

Organism-specific databases

CTDi5923.
RGDi620395. Rasgrf1.

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiP28818.
KOiK04349.
PhylomeDBiP28818.

Miscellaneous databases

PROiP28818.

Family and domain databases

CDDicd00155. RasGEF. 1 hit.
Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 3 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGRF1_RAT
AccessioniPrimary (citable) accession number: P28818
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.