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Protein

3-hydroxyisobutyryl-CoA hydrolase, mitochondrial

Gene

EHD3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane (PubMed:14566057, PubMed:25609543, PubMed:28154081). mS47 has enzymatic activity in vitro, and is able to catalyze the specific hydrolysis of 3-hydroxyisobutyryl-CoA (HIBYL-CoA). However, because the turnover rate of mS47/EHD3 is only a fraction of that of the homologous mammalian enzyme, the physiological function of this activity remains unclear (PubMed:12697341). Has an indirect role in endocytic membrane trafficking (PubMed:11378903).3 Publications2 Publications

Miscellaneous

Present with 3950 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

3-hydroxy-2-methylpropanoyl-CoA + H2O = CoA + 3-hydroxy-2-methylpropanoate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei124SubstrateBy similarity1
Binding sitei149Substrate; via amide nitrogenBy similarity1
Binding sitei172SubstrateBy similarity1
Binding sitei180SubstrateBy similarity1

GO - Molecular functioni

  • 3-hydroxyisobutyryl-CoA hydrolase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Ribonucleoprotein, Ribosomal protein
Biological processBranched-chain amino acid catabolism

Enzyme and pathway databases

BioCyciYEAST:G3O-29650-MONOMER
ReactomeiR-SCE-70895 Branched-chain amino acid catabolism

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyisobutyryl-CoA hydrolase, mitochondrial (EC:3.1.2.4)
Alternative name(s):
3-hydroxyisobutyryl-coenzyme A hydrolase
Short name:
HIB-CoA hydrolase
Short name:
HIBYL-CoA-H
Mitochondrial small ribosomal subunit protein mS471 Publication
Gene namesi
Name:EHD3
Synonyms:MRP5
Ordered Locus Names:YDR036C
ORF Names:YD9673.08C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR036C
SGDiS000002443 EHD3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001093571 – 5003-hydroxyisobutyryl-CoA hydrolase, mitochondrialAdd BLAST500

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei326PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28817
PaxDbiP28817
PRIDEiP28817

PTM databases

iPTMnetiP28817

Interactioni

Subunit structurei

Component of the mitochondrial small ribosomal subunit (mt-SSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins. mS47 forms a protuberance of the yeast mitoribosome and retains a solvent-exposed cavity liekly capable of accommodating a substrate, in accordance with it being an active enzyme as well as an integral constituent of the mitoribosome.1 Publication

Protein-protein interaction databases

BioGridi32091, 54 interactors
ComplexPortaliCPX-1603 37S mitochondrial small ribosomal subunit
DIPiDIP-6509N
IntActiP28817, 24 interactors
MINTiP28817
STRINGi4932.YDR036C

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MRCelectron microscopy3.258836-492[»]
5MREelectron microscopy3.758836-492[»]
5MRFelectron microscopy4.978836-492[»]
ProteinModelPortaliP28817
SMRiP28817
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00890000139491
HOGENOMiHOG000217005
InParanoidiP28817
KOiK05605
OMAiIATHYLH
OrthoDBiEOG092C22B2

Family and domain databases

InterProiView protein in InterPro
IPR029045 ClpP/crotonase-like_dom_sf
IPR018376 Enoyl-CoA_hyd/isom_CS
IPR032259 HIBYL-CoA-H
PfamiView protein in Pfam
PF16113 ECH_2, 1 hit
SUPFAMiSSF52096 SSF52096, 2 hits
PROSITEiView protein in PROSITE
PS00166 ENOYL_COA_HYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

P28817-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRNTLKCAQ LSSKYGFKTT TRTFMTTQPQ LNVTDAPPVL FTVQDTARVI
60 70 80 90 100
TLNRPKKLNA LNAEMSESMF KTLNEYAKSD TTNLVILKSS NRPRSFCAGG
110 120 130 140 150
DVATVAIFNF NKEFAKSIKF FTDEYSLNFQ IATYLKPIVT FMDGITMGGG
160 170 180 190 200
VGLSIHTPFR IATENTKWAM PEMDIGFFPD VGSTFALPRI VTLANSNSQM
210 220 230 240 250
ALYLCLTGEV VTGADAYMLG LASHYVSSEN LDALQKRLGE ISPPFNNDPQ
260 270 280 290 300
SAYFFGMVNE SIDEFVSPLP KDYVFKYSNE KLNVIEACFN LSKNGTIEDI
310 320 330 340 350
MNNLRQYEGS AEGKAFAQEI KTKLLTKSPS SLQIALRLVQ ENSRDHIESA
360 370 380 390 400
IKRDLYTAAN MCMNQDSLVE FSEATKHKLI DKQRVPYPWT KKEQLFVSQL
410 420 430 440 450
TSITSPKPSL PMSLLRNTSN VTWTQYPYHS KYQLPTEQEI AAYIEKRTND
460 470 480 490 500
DTGAKVTERE VLNHFANVIP SRRGKLGIQS LCKIVCERKC EEVNDGLRWK
Length:500
Mass (Da):56,288
Last modified:November 1, 1997 - v2
Checksum:i7187506B4F4BCDC6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti139V → A in AAU09686 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z68196 Genomic DNA Translation: CAA92375.1
Z74332 Genomic DNA Translation: CAA98862.1
AY723769 Genomic DNA Translation: AAU09686.1
J04186 Genomic DNA Translation: AAA66915.1
BK006938 Genomic DNA Translation: DAA11884.1
PIRiS61591
RefSeqiNP_010321.1, NM_001180344.1

Genome annotation databases

EnsemblFungiiYDR036C; YDR036C; YDR036C
GeneIDi851606
KEGGisce:YDR036C

Similar proteinsi

Entry informationi

Entry nameiHIBCH_YEAST
AccessioniPrimary (citable) accession number: P28817
Secondary accession number(s): D6VS24, Q66RH2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: June 20, 2018
This is version 144 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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