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Protein

3-hydroxyisobutyrate dehydrogenase

Gene

mmsB

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH.1 Publication

Pathwayi: L-valine degradation

This protein is involved in the pathway L-valine degradation, which is part of Amino-acid degradation.1 Publication
View all proteins of this organism that are known to be involved in the pathway L-valine degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei96 – 961NADBy similarity
Active sitei171 – 1711By similarity
Binding sitei246 – 2461NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi2 – 3029NADBy similarityAdd
BLAST
Nucleotide bindingi65 – 662NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Branched-chain amino acid catabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00362.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyisobutyrate dehydrogenase1 Publication (EC:1.1.1.311 Publication)
Short name:
HIBADHCurated
Gene namesi
Name:mmsB1 Publication
Ordered Locus Names:PA3569
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 2982983-hydroxyisobutyrate dehydrogenasePRO_0000173058Add
BLAST

Proteomic databases

PaxDbiP28811.
PRIDEiP28811.

Expressioni

Inductioni

By valine.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi208964.PA3569.

Structurei

3D structure databases

ProteinModelPortaliP28811.
SMRiP28811. Positions 1-294.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CF3. Bacteria.
COG2084. LUCA.
HOGENOMiHOG000219610.
InParanoidiP28811.
KOiK00020.
OMAiPAGQHVE.
OrthoDBiEOG6XDH0X.
PhylomeDBiP28811.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR011548. HIBADH.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01692. HIBADH. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28811-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDIAFLGLG NMGGPMAANL LKAGHRVNVF DLQPKAVLGL VEQGAQGADS
60 70 80 90 100
ALQCCEGAEV VISMLPAGQH VESLYLGDDG LLARVAGKPL LIDCSTIAPE
110 120 130 140 150
TARKVAEAAA AKGLTLLDAP VSGGVGGARA GTLSFIVGGP AEGFARARPV
160 170 180 190 200
LENMGRNIFH AGDHGAGQVA KICNNMLLGI LMAGTAEALA LGVKNGLDPA
210 220 230 240 250
VLSEVMKQSS GGNWALNLYN PWPGVMPQAP ASNGYAGGFQ VRLMNKDLGL
260 270 280 290
ALANAQAVQA STPLGALARN LFSLHAQADA EHEGLDFSSI QKLYRGKD
Length:298
Mass (Da):30,486
Last modified:December 1, 1992 - v1
Checksum:i0C4D7B5A7C870730
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84911 Genomic DNA. Translation: AAA25892.1.
AE004091 Genomic DNA. Translation: AAG06957.1.
PIRiC42902.
RefSeqiNP_252259.1. NC_002516.2.
WP_003113890.1. NC_002516.2.

Genome annotation databases

EnsemblBacteriaiAAG06957; AAG06957; PA3569.
GeneIDi879097.
KEGGipae:PA3569.
PATRICi19841755. VBIPseAer58763_3735.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84911 Genomic DNA. Translation: AAA25892.1.
AE004091 Genomic DNA. Translation: AAG06957.1.
PIRiC42902.
RefSeqiNP_252259.1. NC_002516.2.
WP_003113890.1. NC_002516.2.

3D structure databases

ProteinModelPortaliP28811.
SMRiP28811. Positions 1-294.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3569.

Proteomic databases

PaxDbiP28811.
PRIDEiP28811.

Protocols and materials databases

DNASUi879097.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06957; AAG06957; PA3569.
GeneIDi879097.
KEGGipae:PA3569.
PATRICi19841755. VBIPseAer58763_3735.

Organism-specific databases

PseudoCAPiPA3569.

Phylogenomic databases

eggNOGiENOG4105CF3. Bacteria.
COG2084. LUCA.
HOGENOMiHOG000219610.
InParanoidiP28811.
KOiK00020.
OMAiPAGQHVE.
OrthoDBiEOG6XDH0X.
PhylomeDBiP28811.

Enzyme and pathway databases

UniPathwayiUPA00362.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR002204. 3-OH-isobutyrate_DH-rel_CS.
IPR008927. 6-PGluconate_DH_C-like.
IPR013328. 6PGD_dom_2.
IPR006115. 6PGDH_NADP-bd.
IPR011548. HIBADH.
IPR015815. HIBADH-related.
IPR016040. NAD(P)-bd_dom.
IPR029154. NADP-bd.
[Graphical view]
PfamiPF14833. NAD_binding_11. 1 hit.
PF03446. NAD_binding_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000103. HIBADH. 1 hit.
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01692. HIBADH. 1 hit.
PROSITEiPS00895. 3_HYDROXYISOBUT_DH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase."
    Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R.
    J. Biol. Chem. 267:13585-13592(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PATHWAY, INDUCTION.
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.

Entry informationi

Entry nameiMMSB_PSEAE
AccessioniPrimary (citable) accession number: P28811
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.