Reviewed, UniProtKB/Swiss-Prot P28810 (MMSA_PSEAE)

Names and origin

Protein names

Recommended name:
    Methylmalonate-semialdehyde dehydrogenase [acylating]
      Short name=MMSDH
    EC=1.2.1.27

Gene names

Name:	mmsA
Ordered Locus Names:	PA3570

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	497 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	2-methyl-3-oxopropanoate + CoA + H2O + NAD+ = propanoyl-CoA + HCO3- + NADH.
Pathway	Amino-acid degradation; L-valine degradation.
Subunit structure	Homodimer.
Induction	By valine.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW valine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	methylmalonate-semialdehyde dehydrogenase (acylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 497	496	Methylmalonate-semialdehyde dehydrogenase [acylating]		PRO_0000056584
Sites
	Active site	282	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28810-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 3DB6BFC300AACA4D	FASTA	497	53,663
	10 20 30 40 50 60 MSVPVRHLIA GAFVEGLGAQ RIPVSNPLDN STLAEIACAS AEQVEQAVAS ARETFASWKE 70 80 90 100 110 120 TPVSERARVM LRYQALLKEH HDELAKIVSS ELGKTFEDAK GDVWRGIEVV EHACNVPSLL 130 140 150 160 170 180 MGETVENVAR NIDTYSITQP LGVCVGITPF NFPAMIPLWM FPLAIACGNA FILKPSEQVP 190 200 210 220 230 240 LTSVRLAELF LEAGAPKGVL QVVHGGKEQV DQLLKHPQVK AVSFVGSVAV GQYVYHTGTA 250 260 270 280 290 300 HNKRVQSFAG AKNHMVIMPD ADKAQVISNL VGASVGAAGQ RCMAISVAVL VGAAREWIPE 310 320 330 340 350 360 IRDALAKVRP GPWDDSGASY GPVINPQAKA RIERLIGQGV EEGAQLLLDG RGYKVEGYPD 370 380 390 400 410 420 GNWVGPTLFA GVRPDMAIYR EEVFGPVLCL AEVDSLEQAI RLINESPYGN GTSIFTSSGA 430 440 450 460 470 480 AARTFQHHIE VGQVGINIPI PVPLPFFSFT GWKGSFYGDL HAYGKQGVRF YTETKTVTAR 490 WFDSDSVAGT NFSIQMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the mmsAB operon of Pseudomonas aeruginosa PAO encoding methylmalonate-semialdehyde dehydrogenase and 3-hydroxyisobutyrate dehydrogenase." Steele M.I., Lorenz D., Hatter K., Park A., Sokatch J.R. J. Biol. Chem. 267:13585-13592(1992) [PubMed: 1339433] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-30. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]	"Biofouling in water treatment systems: effect of membrane properties on biofilm formation." Liddor M. Thesis (2005), Ben-Gurion University, Israel Cited for: PROTEIN SEQUENCE OF 186-197 AND 335-351. Strain: ATCC 33467 / type 1 smooth.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M84911 Genomic DNA. Translation: AAA25891.1. AE004091 Genomic DNA. Translation: AAG06958.1.
PIR	B42902.
RefSeq	NP_252260.1.
3D structure databases
HSSP	HSSP built from PDB template 1BXS based on UniProtKB P51977.
ModBase	Search...
Genome annotation databases
GeneID	878814.
GenomeReviews	Gene locus PA3570 in contig AE004091_GR.
KEGG	pae:PA3570.
Organism-specific databases
PseudoCAP	PA3570.
CMR	Search...
Phylogenomic databases
HOGENOM	P28810.
OMA	P28810. ACASAEQ.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-11663. PAER208964:PA3570-MON.
BRENDA	1.2.1.27. 354.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR010061. MeMal-semiAld_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit. PTHR11699:SF27. MMSDH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01722. MMSDH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MMSA_PSEAE

Accession

Primary (citable) accession number: P28810

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)