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Protein

Thermoregulatory protein LcrF

Gene

lcrF

Organism
Yersinia pestis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Transcriptional activator of the thermally regulated virulent yopE gene. LcrF activity could be modulated by the interaction with an inducer molecule serving as a temperature messenger. The availability of the messenger would in turn be controlled by a temperature-responsive process serving as a cellular thermometer.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi183 – 20220H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • pathogenesis Source: UniProtKB-KW
  • regulation of transcription, DNA-templated Source: CACAO
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation, Virulence

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYPES214092:GKDD-4256-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Thermoregulatory protein LcrF
Gene namesi
Name:lcrF
Synonyms:virF
Ordered Locus Names:YPCD1.49, y5029, y0032, YP_pCD34
Encoded oniPlasmid pCD10 Publication
OrganismiYersinia pestis
Taxonomic identifieri632 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeYersinia
Proteomesi
  • UP000000815 Componenti: Plasmid pCD1
  • UP000001019 Componenti: Plasmid pCD1

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Thermoregulatory protein LcrFPRO_0000194529Add
BLAST

Proteomic databases

PRIDEiP28808.

Interactioni

Protein-protein interaction databases

IntActiP28808. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP28808.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Contains 1 HTH araC/xylS-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000247159.
OMAiSMNESID.
OrthoDBiEOG6SZ1FM.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLEIIKLE WVTPIFKVVE HSQDGLYILL QGQISWQSSG QTYDLDEGNM
60 70 80 90 100
LFLRRGSYAV RCGTKEPCQL LWIPLPGSFL STFLHRFGSL LSEIGRDNST
110 120 130 140 150
PKPLLIFNIS PILSQSIQNL CAILERSDFP SVLTQLRIEE LLLLLAFSSQ
160 170 180 190 200
GTLFLSALRH LGNRPEERLQ KFMEENYLQG WKLSKFAREF GMGLTTFKEL
210 220 230 240 250
FGTVYGISPR AWISERRILY AHQLLLNGKM SIVDIAMEAG FSSQSYFTQS
260 270
YRRRFGCTPS QARLTKIATT G
Length:271
Mass (Da):30,833
Last modified:December 1, 1992 - v1
Checksum:i4014863486DCE282
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86690 Genomic DNA. Translation: AAA25558.1.
AF074612 Genomic DNA. Translation: AAC69782.1.
AF053946 Genomic DNA. Translation: AAC62554.1.
AL117189 Genomic DNA. Translation: CAB54926.1.
AE017043 Genomic DNA. Translation: AAS58553.1.
PIRiA42884.
RefSeqiNP_395183.1. NC_003131.1.
NP_857733.1. NC_004836.1.
NP_857928.1. NC_004839.1.
WP_002212931.1. NZ_LIXY01000017.1.

Genome annotation databases

EnsemblBacteriaiAAS58553; AAS58553; YP_pCD34.
GeneIDi1149292.
1172694.
15151867.
KEGGipg:1149292.
pg:15151867.
ype:YPCD1.49.
ypl:CH46_4358.
ypm:YP_pCD34.
ypv:BZ15_4404.
ypw:CH59_4398.
PATRICi18588626. VBIYerPes7843_0058.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86690 Genomic DNA. Translation: AAA25558.1.
AF074612 Genomic DNA. Translation: AAC69782.1.
AF053946 Genomic DNA. Translation: AAC62554.1.
AL117189 Genomic DNA. Translation: CAB54926.1.
AE017043 Genomic DNA. Translation: AAS58553.1.
PIRiA42884.
RefSeqiNP_395183.1. NC_003131.1.
NP_857733.1. NC_004836.1.
NP_857928.1. NC_004839.1.
WP_002212931.1. NZ_LIXY01000017.1.

3D structure databases

ProteinModelPortaliP28808.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28808. 3 interactions.

Proteomic databases

PRIDEiP28808.

Protocols and materials databases

DNASUi1149292.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAS58553; AAS58553; YP_pCD34.
GeneIDi1149292.
1172694.
15151867.
KEGGipg:1149292.
pg:15151867.
ype:YPCD1.49.
ypl:CH46_4358.
ypm:YP_pCD34.
ypv:BZ15_4404.
ypw:CH59_4398.
PATRICi18588626. VBIYerPes7843_0058.

Phylogenomic databases

HOGENOMiHOG000247159.
OMAiSMNESID.
OrthoDBiEOG6SZ1FM.

Enzyme and pathway databases

BioCyciYPES214092:GKDD-4256-MONOMER.

Family and domain databases

Gene3Di1.10.10.60. 2 hits.
2.60.120.10. 1 hit.
InterProiIPR003313. AraC-bd.
IPR009057. Homeodomain-like.
IPR018060. HTH_AraC.
IPR018062. HTH_AraC-typ_CS.
IPR014710. RmlC-like_jellyroll.
IPR020449. Tscrpt_reg_HTH_AraC-type.
[Graphical view]
PfamiPF02311. AraC_binding. 1 hit.
PF12833. HTH_18. 1 hit.
[Graphical view]
PRINTSiPR00032. HTHARAC.
SMARTiSM00342. HTH_ARAC. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF51215. SSF51215. 1 hit.
PROSITEiPS00041. HTH_ARAC_FAMILY_1. 1 hit.
PS01124. HTH_ARAC_FAMILY_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Temperature sensing in Yersinia pestis: regulation of yopE transcription by lcrF."
    Goguen J.D., Minion C., Hoe N.P.
    J. Bacteriol. 174:4275-4286(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "DNA sequencing and analysis of the low-Ca2+-response plasmid pCD1 of Yersinia pestis KIM5."
    Perry R.D., Straley S.C., Fetherston J.D., Rose D.J., Gregor J., Blattner F.R.
    Infect. Immun. 66:4611-4623(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KIM5 / Biovar Mediaevalis.
  3. "Structural organization of virulence-associated plasmids of Yersinia pestis."
    Hu P., Elliott J., McCready P., Skowronski E., Garnes J., Kobayashi A., Brubaker R.R., Garcia E.
    J. Bacteriol. 180:5192-5202(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: KIM5 / Biovar Mediaevalis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CO-92 / Biovar Orientalis.
  5. "Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans."
    Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R.
    , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R.
    DNA Res. 11:179-197(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 91001 / Biovar Mediaevalis.

Entry informationi

Entry nameiLCRF_YERPE
AccessioniPrimary (citable) accession number: P28808
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 11, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Plasmid, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.