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P28801

- GSTP1_BOVIN

UniProt

P28801 - GSTP1_BOVIN

Protein

Glutathione S-transferase P

Gene

GSTP1

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration By similarity.By similarity

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei8 – 81GlutathioneBy similarity
    Binding sitei14 – 141GlutathioneBy similarity
    Binding sitei39 – 391GlutathioneBy similarity
    Binding sitei45 – 451GlutathioneBy similarity

    GO - Molecular functioni

    1. dinitrosyl-iron complex binding Source: Ensembl
    2. glutathione transferase activity Source: UniProtKB-EC
    3. S-nitrosoglutathione binding Source: Ensembl

    GO - Biological processi

    1. glutathione metabolic process Source: Ensembl
    2. negative regulation of ERK1 and ERK2 cascade Source: Ensembl
    3. negative regulation of extrinsic apoptotic signaling pathway Source: Ensembl
    4. negative regulation of interleukin-1 beta production Source: Ensembl
    5. negative regulation of JUN kinase activity Source: Ensembl
    6. negative regulation of monocyte chemotactic protein-1 production Source: Ensembl
    7. negative regulation of nitric-oxide synthase biosynthetic process Source: Ensembl
    8. negative regulation of tumor necrosis factor production Source: Ensembl
    9. xenobiotic metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Transferase

    Enzyme and pathway databases

    ReactomeiREACT_204534. Detoxification of Reactive Oxygen Species.
    REACT_223659. Glutathione conjugation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase P (EC:2.5.1.18)
    Alternative name(s):
    GST class-pi
    Gene namesi
    Name:GSTP1
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 29

    Subcellular locationi

    Cytoplasm By similarity. Mitochondrion By similarity. Nucleus By similarity
    Note: The 83 N-terminal amino acids function as un uncleaved transit peptide, and arginine residues within it are crucial for mitochondrial localization.By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell
    3. TRAF2-GSTP1 complex Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Mitochondrion, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 210209Glutathione S-transferase PPRO_0000185896Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei4 – 41Phosphotyrosine; by EGFRBy similarity
    Modified residuei103 – 1031N6-succinyllysineBy similarity
    Modified residuei116 – 1161N6-succinyllysineBy similarity
    Modified residuei128 – 1281N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PaxDbiP28801.
    PRIDEiP28801.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CDK5 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP28801.
    SMRiP28801. Positions 1-210.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 8180GST N-terminalAdd
    BLAST
    Domaini83 – 204122GST C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni52 – 532Glutathione bindingBy similarity
    Regioni65 – 662Glutathione bindingBy similarity

    Sequence similaritiesi

    Belongs to the GST superfamily. Pi family.Curated
    Contains 1 GST C-terminal domain.Curated
    Contains 1 GST N-terminal domain.Curated

    Phylogenomic databases

    eggNOGiNOG05174.
    GeneTreeiENSGT00550000074559.
    HOGENOMiHOG000115733.
    HOVERGENiHBG108324.
    InParanoidiP28801.
    KOiK00799.
    OMAiMNRPING.
    OrthoDBiEOG7KH9M3.
    TreeFamiTF105321.

    Family and domain databases

    Gene3Di1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProiIPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view]
    PfamiPF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view]
    PRINTSiPR01268. GSTRNSFRASEP.
    SUPFAMiSSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEiPS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28801-1 [UniParc]FASTAAdd to Basket

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    MPPYTIVYFP VQGRCEAMRM LLADQGQSWK EEVVAMQSWL QGPLKASCLY    50
    GQLPKFQDGD LTLYQSNAIL RHLGRTLGLY GKDQQEAALV DMVNDGVEDL 100
    RCKYVSLIYT NYEAGKEDYV KALPQHLKPF ETLLSQNKGG QAFIVGDQIS 150
    FADYNLLDLL RIHQVLAPSC LDSFPLLSAY VARLNSRPKL KAFLASPEHM 200
    NRPINGNGKQ 210
    Length:210
    Mass (Da):23,613
    Last modified:January 23, 2007 - v2
    Checksum:i79C45DA2031B1EBB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61233 mRNA. Translation: CAA43551.1.
    BC102704 mRNA. Translation: AAI02705.1.
    PIRiA49180.
    RefSeqiNP_803482.1. NM_177516.1.
    UniGeneiBt.13949.

    Genome annotation databases

    EnsembliENSBTAT00000004615; ENSBTAP00000004615; ENSBTAG00000003548.
    GeneIDi281806.
    KEGGibta:281806.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X61233 mRNA. Translation: CAA43551.1 .
    BC102704 mRNA. Translation: AAI02705.1 .
    PIRi A49180.
    RefSeqi NP_803482.1. NM_177516.1.
    UniGenei Bt.13949.

    3D structure databases

    ProteinModelPortali P28801.
    SMRi P28801. Positions 1-210.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P28801.
    PRIDEi P28801.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000004615 ; ENSBTAP00000004615 ; ENSBTAG00000003548 .
    GeneIDi 281806.
    KEGGi bta:281806.

    Organism-specific databases

    CTDi 2950.

    Phylogenomic databases

    eggNOGi NOG05174.
    GeneTreei ENSGT00550000074559.
    HOGENOMi HOG000115733.
    HOVERGENi HBG108324.
    InParanoidi P28801.
    KOi K00799.
    OMAi MNRPING.
    OrthoDBi EOG7KH9M3.
    TreeFami TF105321.

    Enzyme and pathway databases

    Reactomei REACT_204534. Detoxification of Reactive Oxygen Species.
    REACT_223659. Glutathione conjugation.

    Miscellaneous databases

    NextBioi 20805719.

    Family and domain databases

    Gene3Di 1.20.1050.10. 1 hit.
    3.40.30.10. 1 hit.
    InterProi IPR010987. Glutathione-S-Trfase_C-like.
    IPR004045. Glutathione_S-Trfase_N.
    IPR004046. GST_C.
    IPR003082. GST_pi.
    IPR012336. Thioredoxin-like_fold.
    [Graphical view ]
    Pfami PF00043. GST_C. 1 hit.
    PF02798. GST_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01268. GSTRNSFRASEP.
    SUPFAMi SSF47616. SSF47616. 1 hit.
    SSF52833. SSF52833. 1 hit.
    PROSITEi PS50405. GST_CTER. 1 hit.
    PS50404. GST_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of a cDNA encoding a glutathione S-transferase (GST) class-pi from the bovine ocular ciliary epithelium."
      Hernando N., Martin-Alonso J.M., Ghosh S., Coca-Prados M.
      Exp. Eye Res. 55:711-718(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Ocular ciliary epithelium.
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Crossbred X Angus.
      Tissue: Ileum.
    3. "Glutathione transferase from bovine placenta. Preparation, biochemical characterization, crystallization, and preliminary crystallographic analysis of a neutral class PI enzyme."
      Schaeffer J., Gallay O., Ladenstein R.
      J. Biol. Chem. 263:17405-17411(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-16, SUBUNIT.
      Tissue: Placenta.
    4. "Bovine erythrocyte glutathione S-transferase: purification, inhibition, and complex formation."
      Xu F., Hultquist D.E.
      Biochem. Int. 27:265-274(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 3-23.
      Tissue: Erythrocyte.

    Entry informationi

    Entry nameiGSTP1_BOVIN
    AccessioniPrimary (citable) accession number: P28801
    Secondary accession number(s): Q3SZU6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3