Reviewed, UniProtKB/Swiss-Prot P28798 (GRN_MOUSE)

Names and origin

Protein names

Recommended name:
    Granulins
Alternative name(s):
    Proepithelin
      Short name=PEPI
    PC cell-derived growth factor
      Short name=PCDGF
Cleaved into the following 8 chains:
    1- Recommended name:
            Acrogranin
    2- Recommended name:
            Granulin-1
    3- Recommended name:
            Granulin-2
    4- Recommended name:
            Granulin-3
    5- Recommended name:
            Granulin-4
    6- Recommended name:
            Granulin-5
    7- Recommended name:
            Granulin-6
    8- Recommended name:
            Granulin-7

Gene names

Name:

Grn

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	589 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Granulins have possible cytokine-like activity. They may play a role in inflammation, wound repair, and tissue remodeling. Acts as an autocrine growth factor for PC cells. Ref.4
Subcellular location	Secreted. Ref.4
Tissue specificity	Ubiquitous.
Post-translational modification	Granulins are disulfide bridged. N-glycosylated. Ref.4
Sequence similarities	Belongs to the granulin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blastocyst hatching Inferred from direct assay. Source: MGI embryo implantation Inferred from direct assay. Source: MGI positive regulation of epithelial cell proliferation Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from direct assay. Source: MGI mitochondrion Inferred from direct assay. Source: MGI
Molecular function	cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Ref.4
	Chain	18 – 589	572	Acrogranin		PRO_0000012702
	Peptide	?58 – ?113	56	Granulin-1		PRO_0000012703
	Peptide	?122 – ?178	57	Granulin-2		PRO_0000012704
	Peptide	205 – 260	56	Granulin-3		PRO_0000012705
	Peptide	280 – 334	55	Granulin-4		PRO_0000012706
	Peptide	362 – ?414	53	Granulin-5		PRO_0000012707
	Peptide	440 – ?493	54	Granulin-6		PRO_0000012708
	Peptide	?517 – ?568	52	Granulin-7		PRO_0000012709
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	263	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	526	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	251	1	V → L in AAA37191. Ref.3
	Sequence conflict	254	1	L → V in AAA37191. Ref.3
	Sequence conflict	261 – 263	3	SKN → YD AA sequence Ref.4
	Sequence conflict	350	1	L → R in CAA44197. Ref.2
	Sequence conflict	402 – 403	2	CP → SA in AAA37191. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P28798-1 [UniParc]. Last modified October 1, 1994. Version 2. Checksum: 1DE8229C413CB787	FASTA	589	63,458
	10 20 30 40 50 60 MWVLMSWLAF AAGLVAGTQC PDGQFCPVAC CLDQGGANYS CCNPLLDTWP RITSHHLDGS 70 80 90 100 110 120 CQTHGHCPAG YSCLLTVSGT SSCCPFSKGV SCGDGYHCCP QGFHCSADGK SCFQMSDNPL 130 140 150 160 170 180 GAVQCPGSQF ECPDSATCCI MVDGSWGCCP MPQASCCEDR VHCCPHGASC DLVHTRCVSP 190 200 210 220 230 240 TGTHTLLKKF PAQKTNRAVS LPFSVVCPDA KTQCPDDSTC CELPTGKYGC CPMPNAICCS 250 260 270 280 290 300 DHLHCCPQDT VCDLIQSKCL SKNYTTDLLT KLPGYPVKEV KCDMEVSCPE GYTCCRLNTG 310 320 330 340 350 360 AWGCCPFAKA VCCEDHIHCC PAGFQCHTEK GTCEMGILQV PWMKKVIAPL RLPDPQILKS 370 380 390 400 410 420 DTPCDDFTRC PTNNTCCKLN SGDWGCCPIP EAVCCSDNQH CCPQGFTCLA QGYCQKGDTM 430 440 450 460 470 480 VAGLEKIPAR QTTPLQIGDI GCDQHTSCPV GQTCCPSLKG SWACCQLPHA VCCEDRQHCC 490 500 510 520 530 540 PAGYTCNVKA RTCEKDVDFI QPPVLLTLGP KVGNVECGEG HFCHDNQTCC KDSAGVWACC 550 560 570 580 PYLKGVCCRD GRHCCPGGFH CSARGTKCLR KKIPRWDMFL RDPVPRPLL

References

[1]	"Exon/intron organization of the gene encoding the mouse epithelin/granulin precursor (acrogranin)." Baba T., Nemoto H., Watanabe K., Arai Y., Gerton G.L. FEBS Lett. 322:89-94(1993) [PubMed: 8482392] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"The epithelin precursor encodes two proteins with opposing activities on epithelial cell growth." Plowman G.D., Green J.M., Neubauer M.G., Buckley S.D., McDonald V.L., Todaro G.J., Shoyab M. J. Biol. Chem. 267:13073-13078(1992) [PubMed: 1618805] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Kidney.
[3]	"Acrogranin, an acrosomal cysteine-rich glycoprotein, is the precursor of the growth-modulating peptides, granulins, and epithelins, and is expressed in somatic as well as male germ cells." Baba T., Hoff H.B. III, Nemoto H., Lee H., Orth J., Arai Y., Gerton G.L. Mol. Reprod. Dev. 34:233-243(1993) [PubMed: 8471244] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Purification of an autocrine growth factor homologous with mouse epithelin precursor from a highly tumorigenic cell line." Zhou J., Gao G., Crabb J.W., Serrero G. J. Biol. Chem. 268:10863-10869(1993) [PubMed: 8496151] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-32; 116-127; 152-164; 198-226; 228-233; 259-280; 297-304 AND 421-430, FUNCTION, GLYCOSYLATION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D16195 Genomic DNA. Translation: BAA03736.1. M86736 mRNA. Translation: AAA37191.1. X62321 mRNA. Translation: CAA44197.1.
IPI	IPI00124640.
PIR	C38128.
UniGene	Mm.1568
3D structure databases
HSSP	HSSP built from PDB template 1G26 based on UniProtKB P28799.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28798. 1 interaction.
STRING	P28798.
Proteomic databases
PRIDE	P28798.
Genome annotation databases
Ensembl	ENSMUST00000049460; ENSMUSP00000046340; ENSMUSG00000034708; Mus musculus. [Genome view]
UCSC	uc007lrv.1. mouse.
Organism-specific databases
MGI	MGI:95832. Grn.
Phylogenomic databases
HOVERGEN	P28798.
Gene expression databases
ArrayExpress	P28798.
Bgee	P28798.
CleanEx	MM_GRN.
Genevestigator	P28798.
GermOnline	ENSMUSG00000034708. Mus musculus.
Family and domain databases
InterPro	IPR000118. Granulin. [Graphical view]
Pfam	PF00396. Granulin. 7 hits. [Graphical view]
SMART	SM00277. GRAN. 7 hits. [Graphical view]
PROSITE	PS00799. GRANULINS. 7 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	287027.
SOURCE	Search...

Entry information

Entry name

GRN_MOUSE

Accession

Primary (citable) accession number: P28798

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	October 1, 1994
Last modified:	November 3, 2009
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)