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P28791

- SEC20_YEAST

UniProt

P28791 - SEC20_YEAST

Protein

Protein transport protein SEC20

Gene

SEC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    SNARE required for targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. SNAP receptor activity Source: SGD

    GO - Biological processi

    1. protein transport Source: UniProtKB-KW
    2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
    3. vesicle fusion with endoplasmic reticulum Source: SGD

    Keywords - Biological processi

    ER-Golgi transport, Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30021-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein transport protein SEC20
    Gene namesi
    Name:SEC20
    Ordered Locus Names:YDR498C
    ORF Names:D9719.4
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDR498c.
    SGDiS000002906. SEC20.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 PublicationPROSITE-ProRule annotation; Single-pass type IV membrane protein 1 Publication

    GO - Cellular componenti

    1. endoplasmic reticulum Source: SGD
    2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: SGD
    4. SNARE complex Source: SGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 383383Protein transport protein SEC20PRO_0000097659Add
    BLAST

    Post-translational modificationi

    O-glycosylated, but not N-glycosylated.Curated

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP28791.
    PaxDbiP28791.

    Expressioni

    Gene expression databases

    GenevestigatoriP28791.

    Interactioni

    Subunit structurei

    Component of a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6. Interacts with TIP20 through its cytoplasmic domain.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TIP20P338916EBI-16572,EBI-19396
    USE1P531464EBI-16572,EBI-23881

    Protein-protein interaction databases

    BioGridi32549. 23 interactions.
    DIPiDIP-5844N.
    IntActiP28791. 15 interactions.
    MINTiMINT-2732319.
    STRINGi4932.YDR498C.

    Structurei

    3D structure databases

    ProteinModelPortaliP28791.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 275275CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini293 – 38391LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei276 – 29217Helical; Anchor for type IV membrane proteinAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili6 – 2621Sequence AnalysisAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi380 – 3834Prevents secretion from ER

    Sequence similaritiesi

    Belongs to the SEC20 family.Curated

    Keywords - Domaini

    Coiled coil, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG324980.
    HOGENOMiHOG000154280.
    KOiK08497.
    OrthoDBiEOG7CNZS5.

    Family and domain databases

    InterProiIPR005606. Sec20.
    [Graphical view]
    PfamiPF03908. Sec20. 1 hit.
    [Graphical view]
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28791-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVVTFLQDLE VLQDALLNNL QKLSAISRRK ESGESKHDNK DSFAAIANEH    50
    NDEEEEIEFE DLVNIIESKV SDFESVLKCS IVEMTYKYPE LKLQWEKSPR 100
    YDQCDKLHIV KLDKQMNEDI YAQLVEELDF VLQFVDWFYC YRLKVKEILR 150
    QHHKRDLAWN DEKRDRAIKF HAVDYDKLHQ GTSSSSSLTS TSMEKASTRE 200
    KLLSKTKQLT NNLVRGNQIL QSGILQSDLN LDELRAQTNS LTQIDDKYTQ 250
    FETVFKKTAD LVKVLENASH QEKRDVYLSL GFLLCCVSWV LWRRIFKLPV 300
    KLGLWLLFKF FKGILVTLGL VKSYAGSSSS LQAPSLVLNA PILATTTTSS 350
    ATSVEPFASV SAVSSIQRAV DEAVDRIVSH DEL 383
    Length:383
    Mass (Da):43,882
    Last modified:December 1, 1992 - v1
    Checksum:i9BF8C748B8A8F589
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60215 Genomic DNA. Translation: CAA42776.1.
    U33057 Genomic DNA. Translation: AAB64940.1.
    AY693133 Genomic DNA. Translation: AAT93152.1.
    BK006938 Genomic DNA. Translation: DAA12330.1.
    PIRiS19633.
    RefSeqiNP_010786.3. NM_001180806.3.

    Genome annotation databases

    EnsemblFungiiYDR498C; YDR498C; YDR498C.
    GeneIDi852109.
    KEGGisce:YDR498C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60215 Genomic DNA. Translation: CAA42776.1 .
    U33057 Genomic DNA. Translation: AAB64940.1 .
    AY693133 Genomic DNA. Translation: AAT93152.1 .
    BK006938 Genomic DNA. Translation: DAA12330.1 .
    PIRi S19633.
    RefSeqi NP_010786.3. NM_001180806.3.

    3D structure databases

    ProteinModelPortali P28791.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 32549. 23 interactions.
    DIPi DIP-5844N.
    IntActi P28791. 15 interactions.
    MINTi MINT-2732319.
    STRINGi 4932.YDR498C.

    Proteomic databases

    MaxQBi P28791.
    PaxDbi P28791.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDR498C ; YDR498C ; YDR498C .
    GeneIDi 852109.
    KEGGi sce:YDR498C.

    Organism-specific databases

    CYGDi YDR498c.
    SGDi S000002906. SEC20.

    Phylogenomic databases

    eggNOGi NOG324980.
    HOGENOMi HOG000154280.
    KOi K08497.
    OrthoDBi EOG7CNZS5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30021-MONOMER.

    Miscellaneous databases

    NextBioi 970469.

    Gene expression databases

    Genevestigatori P28791.

    Family and domain databases

    InterProi IPR005606. Sec20.
    [Graphical view ]
    Pfami PF03908. Sec20. 1 hit.
    [Graphical view ]
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein which is sorted by the HDEL retrieval system."
      Sweet D.J., Pelham H.R.B.
      EMBO J. 11:423-432(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. "Use1p is a yeast SNARE protein required for retrograde traffic to the ER."
      Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D., Fischer von Mollard G.
      EMBO J. 22:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH UFE1; SEC22 AND USE1.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiSEC20_YEAST
    AccessioniPrimary (citable) accession number: P28791
    Secondary accession number(s): D6VTC0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4910 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3