Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P28791 (SEC20_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein transport protein SEC20
Gene names
Name:SEC20
Ordered Locus Names:YDR498C
ORF Names:D9719.4
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length383 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

SNARE required for targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Ref.5

Subunit structure

Component of a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6. Interacts with TIP20 through its cytoplasmic domain. Ref.5

Subcellular location

Endoplasmic reticulum membrane; Single-pass type IV membrane protein Ref.1.

Post-translational modification

O-glycosylated, but not N-glycosylated Probable.

Miscellaneous

Present with 4910 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SEC20 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TIP20P338916EBI-16572,EBI-19396
USE1P531464EBI-16572,EBI-23881

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 383383Protein transport protein SEC20
PRO_0000097659

Regions

Topological domain1 – 275275Cytoplasmic Potential
Transmembrane276 – 29217Helical; Anchor for type IV membrane protein
Topological domain293 – 38391Lumenal Potential
Coiled coil6 – 2621 Potential
Motif380 – 3834Prevents secretion from ER

Sequences

Sequence LengthMass (Da)Tools
P28791 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 9BF8C748B8A8F589

FASTA38343,882
        10         20         30         40         50         60 
MVVTFLQDLE VLQDALLNNL QKLSAISRRK ESGESKHDNK DSFAAIANEH NDEEEEIEFE 

        70         80         90        100        110        120 
DLVNIIESKV SDFESVLKCS IVEMTYKYPE LKLQWEKSPR YDQCDKLHIV KLDKQMNEDI 

       130        140        150        160        170        180 
YAQLVEELDF VLQFVDWFYC YRLKVKEILR QHHKRDLAWN DEKRDRAIKF HAVDYDKLHQ 

       190        200        210        220        230        240 
GTSSSSSLTS TSMEKASTRE KLLSKTKQLT NNLVRGNQIL QSGILQSDLN LDELRAQTNS 

       250        260        270        280        290        300 
LTQIDDKYTQ FETVFKKTAD LVKVLENASH QEKRDVYLSL GFLLCCVSWV LWRRIFKLPV 

       310        320        330        340        350        360 
KLGLWLLFKF FKGILVTLGL VKSYAGSSSS LQAPSLVLNA PILATTTTSS ATSVEPFASV 

       370        380 
SAVSSIQRAV DEAVDRIVSH DEL 

« Hide

References

« Hide 'large scale' references
[1]"The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein which is sorted by the HDEL retrieval system."
Sweet D.J., Pelham H.R.B.
EMBO J. 11:423-432(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T. expand/collapse author list , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"Use1p is a yeast SNARE protein required for retrograde traffic to the ER."
Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D., Fischer von Mollard G.
EMBO J. 22:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH UFE1; SEC22 AND USE1.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X60215 Genomic DNA. Translation: CAA42776.1.
U33057 Genomic DNA. Translation: AAB64940.1.
AY693133 Genomic DNA. Translation: AAT93152.1.
BK006938 Genomic DNA. Translation: DAA12330.1.
PIRS19633.
RefSeqNP_010786.3. NM_001180806.3.

3D structure databases

ProteinModelPortalP28791.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid32549. 23 interactions.
DIPDIP-5844N.
IntActP28791. 15 interactions.
MINTMINT-2732319.
STRING4932.YDR498C.

Proteomic databases

PaxDbP28791.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYDR498C; YDR498C; YDR498C.
GeneID852109.
KEGGsce:YDR498C.

Organism-specific databases

CYGDYDR498c.
SGDS000002906. SEC20.

Phylogenomic databases

eggNOGNOG324980.
HOGENOMHOG000154280.
KOK08497.
OrthoDBEOG7CNZS5.

Enzyme and pathway databases

BioCycYEAST:G3O-30021-MONOMER.

Gene expression databases

GenevestigatorP28791.

Family and domain databases

InterProIPR005606. Sec20.
[Graphical view]
PfamPF03908. Sec20. 1 hit.
[Graphical view]
PROSITEPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio970469.

Entry information

Entry nameSEC20_YEAST
AccessionPrimary (citable) accession number: P28791
Secondary accession number(s): D6VTC0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: February 19, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome IV

Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families