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Protein

Protein transport protein SEC20

Gene

SEC20

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

SNARE required for targeting and fusion of Golgi-derived retrograde transport vesicles with the ER.1 Publication

GO - Molecular functioni

  1. SNAP receptor activity Source: SGD

GO - Biological processi

  1. protein transport Source: UniProtKB-KW
  2. retrograde vesicle-mediated transport, Golgi to ER Source: SGD
  3. vesicle fusion with endoplasmic reticulum Source: SGD
Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-30021-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein SEC20
Gene namesi
Name:SEC20
Ordered Locus Names:YDR498C
ORF Names:D9719.4
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome IV

Organism-specific databases

CYGDiYDR498c.
EuPathDBiFungiDB:YDR498C.
SGDiS000002906. SEC20.

Subcellular locationi

  1. Endoplasmic reticulum membrane PROSITE-ProRule annotation1 Publication; Single-pass type IV membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 275275CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei276 – 29217Helical; Anchor for type IV membrane proteinAdd
BLAST
Topological domaini293 – 38391LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endoplasmic reticulum Source: SGD
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: SGD
  4. SNARE complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 383383Protein transport protein SEC20PRO_0000097659Add
BLAST

Post-translational modificationi

O-glycosylated, but not N-glycosylated.Curated

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP28791.
PaxDbiP28791.

Expressioni

Gene expression databases

GenevestigatoriP28791.

Interactioni

Subunit structurei

Component of a SNARE complex consisting of UFE1, USE1, SEC20 and SEC22 or YKT6. Interacts with TIP20 through its cytoplasmic domain.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
TIP20P338916EBI-16572,EBI-19396
USE1P531464EBI-16572,EBI-23881

Protein-protein interaction databases

BioGridi32549. 25 interactions.
DIPiDIP-5844N.
IntActiP28791. 15 interactions.
MINTiMINT-2732319.
STRINGi4932.YDR498C.

Structurei

3D structure databases

ProteinModelPortaliP28791.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili6 – 2621Sequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi380 – 3834Prevents secretion from ER

Sequence similaritiesi

Belongs to the SEC20 family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG324980.
HOGENOMiHOG000154280.
InParanoidiP28791.
KOiK08497.
OrthoDBiEOG7CNZS5.

Family and domain databases

InterProiIPR005606. Sec20.
[Graphical view]
PfamiPF03908. Sec20. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28791-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVVTFLQDLE VLQDALLNNL QKLSAISRRK ESGESKHDNK DSFAAIANEH
60 70 80 90 100
NDEEEEIEFE DLVNIIESKV SDFESVLKCS IVEMTYKYPE LKLQWEKSPR
110 120 130 140 150
YDQCDKLHIV KLDKQMNEDI YAQLVEELDF VLQFVDWFYC YRLKVKEILR
160 170 180 190 200
QHHKRDLAWN DEKRDRAIKF HAVDYDKLHQ GTSSSSSLTS TSMEKASTRE
210 220 230 240 250
KLLSKTKQLT NNLVRGNQIL QSGILQSDLN LDELRAQTNS LTQIDDKYTQ
260 270 280 290 300
FETVFKKTAD LVKVLENASH QEKRDVYLSL GFLLCCVSWV LWRRIFKLPV
310 320 330 340 350
KLGLWLLFKF FKGILVTLGL VKSYAGSSSS LQAPSLVLNA PILATTTTSS
360 370 380
ATSVEPFASV SAVSSIQRAV DEAVDRIVSH DEL
Length:383
Mass (Da):43,882
Last modified:December 1, 1992 - v1
Checksum:i9BF8C748B8A8F589
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60215 Genomic DNA. Translation: CAA42776.1.
U33057 Genomic DNA. Translation: AAB64940.1.
AY693133 Genomic DNA. Translation: AAT93152.1.
BK006938 Genomic DNA. Translation: DAA12330.1.
PIRiS19633.
RefSeqiNP_010786.3. NM_001180806.3.

Genome annotation databases

EnsemblFungiiYDR498C; YDR498C; YDR498C.
GeneIDi852109.
KEGGisce:YDR498C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60215 Genomic DNA. Translation: CAA42776.1.
U33057 Genomic DNA. Translation: AAB64940.1.
AY693133 Genomic DNA. Translation: AAT93152.1.
BK006938 Genomic DNA. Translation: DAA12330.1.
PIRiS19633.
RefSeqiNP_010786.3. NM_001180806.3.

3D structure databases

ProteinModelPortaliP28791.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32549. 25 interactions.
DIPiDIP-5844N.
IntActiP28791. 15 interactions.
MINTiMINT-2732319.
STRINGi4932.YDR498C.

Proteomic databases

MaxQBiP28791.
PaxDbiP28791.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDR498C; YDR498C; YDR498C.
GeneIDi852109.
KEGGisce:YDR498C.

Organism-specific databases

CYGDiYDR498c.
EuPathDBiFungiDB:YDR498C.
SGDiS000002906. SEC20.

Phylogenomic databases

eggNOGiNOG324980.
HOGENOMiHOG000154280.
InParanoidiP28791.
KOiK08497.
OrthoDBiEOG7CNZS5.

Enzyme and pathway databases

BioCyciYEAST:G3O-30021-MONOMER.

Miscellaneous databases

NextBioi970469.
PROiP28791.

Gene expression databases

GenevestigatoriP28791.

Family and domain databases

InterProiIPR005606. Sec20.
[Graphical view]
PfamiPF03908. Sec20. 1 hit.
[Graphical view]
PROSITEiPS00014. ER_TARGET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Saccharomyces cerevisiae SEC20 gene encodes a membrane glycoprotein which is sorted by the HDEL retrieval system."
    Sweet D.J., Pelham H.R.B.
    EMBO J. 11:423-432(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, TOPOLOGY.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Use1p is a yeast SNARE protein required for retrograde traffic to the ER."
    Dilcher M., Veith B., Chidambaram S., Hartmann E., Schmitt H.D., Fischer von Mollard G.
    EMBO J. 22:3664-3674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH UFE1; SEC22 AND USE1.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiSEC20_YEAST
AccessioniPrimary (citable) accession number: P28791
Secondary accession number(s): D6VTC0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 29, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4910 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.