Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Porphobilinogen deaminase

Gene

HEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity
Present with 8480 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

dipyrromethaneNote: Binds 1 dipyrromethane group covalently.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase (HEM2)
  2. Porphobilinogen deaminase (HEM3)
  3. Uroporphyrinogen-III synthase (HEM4)
  4. Uroporphyrinogen decarboxylase (HEM12)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

GO - Molecular functioni

  • hydroxymethylbilane synthase activity Source: SGD

GO - Biological processi

  • heme biosynthetic process Source: SGD
  • peptidyl-pyrromethane cofactor linkage Source: InterPro
  • protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

Keywordsi

Molecular functionTransferase
Biological processHeme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YDL205C-MONOMER
ReactomeiR-SCE-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00319

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:HEM3
Ordered Locus Names:YDL205C
ORF Names:D1057
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL205C
SGDiS000002364 HEM3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001430451 – 327Porphobilinogen deaminaseAdd BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei251S-(dipyrrolylmethanemethyl)cysteineBy similarity1

Proteomic databases

MaxQBiP28789
PaxDbiP28789
PRIDEiP28789

PTM databases

iPTMnetiP28789

Interactioni

Protein-protein interaction databases

BioGridi31841, 19 interactors
STRINGi4932.YDL205C

Structurei

3D structure databases

ProteinModelPortaliP28789
SMRiP28789
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000009083
HOGENOMiHOG000228587
InParanoidiP28789
KOiK01749
OMAiCQVPIGV
OrthoDBiEOG092C30OH

Family and domain databases

Gene3Di3.30.160.40, 1 hit
InterProiView protein in InterPro
IPR000860 HemC
IPR022419 Porphobilin_deaminase_cofac_BS
IPR022417 Porphobilin_deaminase_N
IPR022418 Porphobilinogen_deaminase_C
IPR036803 Porphobilinogen_deaminase_C_sf
PANTHERiPTHR11557 PTHR11557, 1 hit
PfamiView protein in Pfam
PF01379 Porphobil_deam, 1 hit
PF03900 Porphobil_deamC, 1 hit
PIRSFiPIRSF001438 4pyrrol_synth_OHMeBilane_synth, 1 hit
PRINTSiPR00151 PORPHBDMNASE
SUPFAMiSSF54782 SSF54782, 1 hit
TIGRFAMsiTIGR00212 hemC, 1 hit
PROSITEiView protein in PROSITE
PS00533 PORPHOBILINOGEN_DEAM, 1 hit

Sequencei

Sequence statusi: Complete.

P28789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPETLHIGG RKSKLAVIQS NHVLKLIEEK YPDYDCKVFT LQTLGDQIQF
60 70 80 90 100
KPLYSFGGKA LWTKELEDHL YHDDPSKKLD LIVHSLKDMP TLLPEGFELG
110 120 130 140 150
GITKRVDPTD CLVMPFYSAY KSLDDLPDGG IVGTSSVRRS AQLKRKYPHL
160 170 180 190 200
KFESVRGNIQ TRLQKLDDPK SPYQCIILAS AGLMRMGLEN RITQRFHSDT
210 220 230 240 250
MYHAVGQGAL GIEIRKGDTK MMKILDEICD LNATICCLSE RALMRTLEGG
260 270 280 290 300
CSVPIGVESK YNEETKKLLL KAIVVDVEGT EAVEDEIEML IENVKEDSMA
310 320
CGKILAERMI ADGAKKILDE INLDRIK
Length:327
Mass (Da):36,675
Last modified:December 1, 1992 - v1
Checksum:i1D22FF3B131ECE73
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11745 Genomic DNA Translation: CAA77804.1
X99000 Genomic DNA Translation: CAA67486.1
Z74253 Genomic DNA Translation: CAA98783.1
AY899249 mRNA Translation: AAX83934.1
BK006938 Genomic DNA Translation: DAA11659.1
PIRiS25071
RefSeqiNP_010076.1, NM_001180265.1

Genome annotation databases

EnsemblFungiiYDL205C; YDL205C; YDL205C
GeneIDi851322
KEGGisce:YDL205C

Similar proteinsi

Entry informationi

Entry nameiHEM3_YEAST
AccessioniPrimary (citable) accession number: P28789
Secondary accession number(s): D6VRE9, Q2VQW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: May 23, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health