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P28789

- HEM3_YEAST

UniProt

P28789 - HEM3_YEAST

Protein

Porphobilinogen deaminase

Gene

HEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

    Catalytic activityi

    4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

    Cofactori

    Binds 1 dipyrromethane group covalently.

    Pathwayi

    GO - Molecular functioni

    1. hydroxymethylbilane synthase activity Source: SGD

    GO - Biological processi

    1. heme biosynthetic process Source: SGD
    2. peptidyl-pyrromethane cofactor linkage Source: InterPro
    3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Enzyme and pathway databases

    BioCyciYEAST:YDL205C-MONOMER.
    UniPathwayiUPA00251; UER00319.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Porphobilinogen deaminase (EC:2.5.1.61)
    Short name:
    PBG
    Alternative name(s):
    Hydroxymethylbilane synthase
    Short name:
    HMBS
    Pre-uroporphyrinogen synthase
    Gene namesi
    Name:HEM3
    Ordered Locus Names:YDL205C
    ORF Names:D1057
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL205c.
    SGDiS000002364. HEM3.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 327327Porphobilinogen deaminasePRO_0000143045Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei251 – 2511S-(dipyrrolylmethanemethyl)cysteineBy similarity

    Proteomic databases

    MaxQBiP28789.
    PaxDbiP28789.
    PeptideAtlasiP28789.
    PRIDEiP28789.

    Expressioni

    Gene expression databases

    GenevestigatoriP28789.

    Interactioni

    Protein-protein interaction databases

    BioGridi31841. 16 interactions.
    MINTiMINT-4479920.
    STRINGi4932.YDL205C.

    Structurei

    3D structure databases

    ProteinModelPortaliP28789.
    SMRiP28789. Positions 6-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the HMBS family.Curated

    Phylogenomic databases

    eggNOGiCOG0181.
    GeneTreeiENSGT00390000009083.
    HOGENOMiHOG000228587.
    KOiK01749.
    OMAiCRSDDET.
    OrthoDBiEOG7S4XGP.

    Family and domain databases

    Gene3Di3.30.160.40. 1 hit.
    InterProiIPR000860. 4pyrrol_synth_OHMeBilane_synth.
    IPR022419. Porphobilin_deaminase_cofac_BS.
    IPR022417. Porphobilin_deaminase_N.
    IPR022418. Porphobilinogen_deaminase_C.
    [Graphical view]
    PANTHERiPTHR11557. PTHR11557. 1 hit.
    PfamiPF01379. Porphobil_deam. 1 hit.
    PF03900. Porphobil_deamC. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
    PRINTSiPR00151. PORPHBDMNASE.
    SUPFAMiSSF54782. SSF54782. 1 hit.
    TIGRFAMsiTIGR00212. hemC. 1 hit.
    PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28789-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGPETLHIGG RKSKLAVIQS NHVLKLIEEK YPDYDCKVFT LQTLGDQIQF    50
    KPLYSFGGKA LWTKELEDHL YHDDPSKKLD LIVHSLKDMP TLLPEGFELG 100
    GITKRVDPTD CLVMPFYSAY KSLDDLPDGG IVGTSSVRRS AQLKRKYPHL 150
    KFESVRGNIQ TRLQKLDDPK SPYQCIILAS AGLMRMGLEN RITQRFHSDT 200
    MYHAVGQGAL GIEIRKGDTK MMKILDEICD LNATICCLSE RALMRTLEGG 250
    CSVPIGVESK YNEETKKLLL KAIVVDVEGT EAVEDEIEML IENVKEDSMA 300
    CGKILAERMI ADGAKKILDE INLDRIK 327
    Length:327
    Mass (Da):36,675
    Last modified:December 1, 1992 - v1
    Checksum:i1D22FF3B131ECE73
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11745 Genomic DNA. Translation: CAA77804.1.
    X99000 Genomic DNA. Translation: CAA67486.1.
    Z74253 Genomic DNA. Translation: CAA98783.1.
    AY899249 mRNA. Translation: AAX83934.1.
    BK006938 Genomic DNA. Translation: DAA11659.1.
    PIRiS25071.
    RefSeqiNP_010076.1. NM_001180265.1.

    Genome annotation databases

    EnsemblFungiiYDL205C; YDL205C; YDL205C.
    GeneIDi851322.
    KEGGisce:YDL205C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11745 Genomic DNA. Translation: CAA77804.1 .
    X99000 Genomic DNA. Translation: CAA67486.1 .
    Z74253 Genomic DNA. Translation: CAA98783.1 .
    AY899249 mRNA. Translation: AAX83934.1 .
    BK006938 Genomic DNA. Translation: DAA11659.1 .
    PIRi S25071.
    RefSeqi NP_010076.1. NM_001180265.1.

    3D structure databases

    ProteinModelPortali P28789.
    SMRi P28789. Positions 6-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31841. 16 interactions.
    MINTi MINT-4479920.
    STRINGi 4932.YDL205C.

    Proteomic databases

    MaxQBi P28789.
    PaxDbi P28789.
    PeptideAtlasi P28789.
    PRIDEi P28789.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL205C ; YDL205C ; YDL205C .
    GeneIDi 851322.
    KEGGi sce:YDL205C.

    Organism-specific databases

    CYGDi YDL205c.
    SGDi S000002364. HEM3.

    Phylogenomic databases

    eggNOGi COG0181.
    GeneTreei ENSGT00390000009083.
    HOGENOMi HOG000228587.
    KOi K01749.
    OMAi CRSDDET.
    OrthoDBi EOG7S4XGP.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00319 .
    BioCyci YEAST:YDL205C-MONOMER.

    Miscellaneous databases

    NextBioi 968366.
    PROi P28789.

    Gene expression databases

    Genevestigatori P28789.

    Family and domain databases

    Gene3Di 3.30.160.40. 1 hit.
    InterProi IPR000860. 4pyrrol_synth_OHMeBilane_synth.
    IPR022419. Porphobilin_deaminase_cofac_BS.
    IPR022417. Porphobilin_deaminase_N.
    IPR022418. Porphobilinogen_deaminase_C.
    [Graphical view ]
    PANTHERi PTHR11557. PTHR11557. 1 hit.
    Pfami PF01379. Porphobil_deam. 1 hit.
    PF03900. Porphobil_deamC. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
    PRINTSi PR00151. PORPHBDMNASE.
    SUPFAMi SSF54782. SSF54782. 1 hit.
    TIGRFAMsi TIGR00212. hemC. 1 hit.
    PROSITEi PS00533. PORPHOBILINOGEN_DEAM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and regulation of yeast HEM3, the gene for porphobilinogen deaminase."
      Keng T., Richard C., Larocque R.
      Mol. Gen. Genet. 234:233-243(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
      Zhang Z., Dietrich F.S.
      Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-97.
      Strain: ATCC 208353 / W303-1A.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHEM3_YEAST
    AccessioniPrimary (citable) accession number: P28789
    Secondary accession number(s): D6VRE9, Q2VQW9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The porphobilinogen subunits are added to the dipyrromethane group.By similarity
    Present with 8480 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3