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P28789

- HEM3_YEAST

UniProt

P28789 - HEM3_YEAST

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Protein

Porphobilinogen deaminase

Gene

HEM3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.

Catalytic activityi

4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH3.

Cofactori

Binds 1 dipyrromethane group covalently.

Pathwayi

GO - Molecular functioni

  1. hydroxymethylbilane synthase activity Source: SGD

GO - Biological processi

  1. heme biosynthetic process Source: SGD
  2. peptidyl-pyrromethane cofactor linkage Source: InterPro
  3. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YDL205C-MONOMER.
UniPathwayiUPA00251; UER00319.

Names & Taxonomyi

Protein namesi
Recommended name:
Porphobilinogen deaminase (EC:2.5.1.61)
Short name:
PBG
Alternative name(s):
Hydroxymethylbilane synthase
Short name:
HMBS
Pre-uroporphyrinogen synthase
Gene namesi
Name:HEM3
Ordered Locus Names:YDL205C
ORF Names:D1057
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome IV

Organism-specific databases

CYGDiYDL205c.
SGDiS000002364. HEM3.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 327327Porphobilinogen deaminasePRO_0000143045Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei251 – 2511S-(dipyrrolylmethanemethyl)cysteineBy similarity

Proteomic databases

MaxQBiP28789.
PaxDbiP28789.
PeptideAtlasiP28789.
PRIDEiP28789.

Expressioni

Gene expression databases

GenevestigatoriP28789.

Interactioni

Protein-protein interaction databases

BioGridi31841. 17 interactions.
MINTiMINT-4479920.
STRINGi4932.YDL205C.

Structurei

3D structure databases

ProteinModelPortaliP28789.
SMRiP28789. Positions 6-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HMBS family.Curated

Phylogenomic databases

eggNOGiCOG0181.
GeneTreeiENSGT00390000009083.
HOGENOMiHOG000228587.
InParanoidiP28789.
KOiK01749.
OMAiCRSDDET.
OrthoDBiEOG7S4XGP.

Family and domain databases

Gene3Di3.30.160.40. 1 hit.
InterProiIPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view]
PANTHERiPTHR11557. PTHR11557. 1 hit.
PfamiPF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view]
PIRSFiPIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSiPR00151. PORPHBDMNASE.
SUPFAMiSSF54782. SSF54782. 1 hit.
TIGRFAMsiTIGR00212. hemC. 1 hit.
PROSITEiPS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28789-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPETLHIGG RKSKLAVIQS NHVLKLIEEK YPDYDCKVFT LQTLGDQIQF
60 70 80 90 100
KPLYSFGGKA LWTKELEDHL YHDDPSKKLD LIVHSLKDMP TLLPEGFELG
110 120 130 140 150
GITKRVDPTD CLVMPFYSAY KSLDDLPDGG IVGTSSVRRS AQLKRKYPHL
160 170 180 190 200
KFESVRGNIQ TRLQKLDDPK SPYQCIILAS AGLMRMGLEN RITQRFHSDT
210 220 230 240 250
MYHAVGQGAL GIEIRKGDTK MMKILDEICD LNATICCLSE RALMRTLEGG
260 270 280 290 300
CSVPIGVESK YNEETKKLLL KAIVVDVEGT EAVEDEIEML IENVKEDSMA
310 320
CGKILAERMI ADGAKKILDE INLDRIK
Length:327
Mass (Da):36,675
Last modified:December 1, 1992 - v1
Checksum:i1D22FF3B131ECE73
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11745 Genomic DNA. Translation: CAA77804.1.
X99000 Genomic DNA. Translation: CAA67486.1.
Z74253 Genomic DNA. Translation: CAA98783.1.
AY899249 mRNA. Translation: AAX83934.1.
BK006938 Genomic DNA. Translation: DAA11659.1.
PIRiS25071.
RefSeqiNP_010076.1. NM_001180265.1.

Genome annotation databases

EnsemblFungiiYDL205C; YDL205C; YDL205C.
GeneIDi851322.
KEGGisce:YDL205C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11745 Genomic DNA. Translation: CAA77804.1 .
X99000 Genomic DNA. Translation: CAA67486.1 .
Z74253 Genomic DNA. Translation: CAA98783.1 .
AY899249 mRNA. Translation: AAX83934.1 .
BK006938 Genomic DNA. Translation: DAA11659.1 .
PIRi S25071.
RefSeqi NP_010076.1. NM_001180265.1.

3D structure databases

ProteinModelPortali P28789.
SMRi P28789. Positions 6-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31841. 17 interactions.
MINTi MINT-4479920.
STRINGi 4932.YDL205C.

Proteomic databases

MaxQBi P28789.
PaxDbi P28789.
PeptideAtlasi P28789.
PRIDEi P28789.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YDL205C ; YDL205C ; YDL205C .
GeneIDi 851322.
KEGGi sce:YDL205C.

Organism-specific databases

CYGDi YDL205c.
SGDi S000002364. HEM3.

Phylogenomic databases

eggNOGi COG0181.
GeneTreei ENSGT00390000009083.
HOGENOMi HOG000228587.
InParanoidi P28789.
KOi K01749.
OMAi CRSDDET.
OrthoDBi EOG7S4XGP.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00319 .
BioCyci YEAST:YDL205C-MONOMER.

Miscellaneous databases

NextBioi 968366.
PROi P28789.

Gene expression databases

Genevestigatori P28789.

Family and domain databases

Gene3Di 3.30.160.40. 1 hit.
InterProi IPR000860. 4pyrrol_synth_OHMeBilane_synth.
IPR022419. Porphobilin_deaminase_cofac_BS.
IPR022417. Porphobilin_deaminase_N.
IPR022418. Porphobilinogen_deaminase_C.
[Graphical view ]
PANTHERi PTHR11557. PTHR11557. 1 hit.
Pfami PF01379. Porphobil_deam. 1 hit.
PF03900. Porphobil_deamC. 1 hit.
[Graphical view ]
PIRSFi PIRSF001438. 4pyrrol_synth_OHMeBilane_synth. 1 hit.
PRINTSi PR00151. PORPHBDMNASE.
SUPFAMi SSF54782. SSF54782. 1 hit.
TIGRFAMsi TIGR00212. hemC. 1 hit.
PROSITEi PS00533. PORPHOBILINOGEN_DEAM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and regulation of yeast HEM3, the gene for porphobilinogen deaminase."
    Keng T., Richard C., Larocque R.
    Mol. Gen. Genet. 234:233-243(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
    Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
    , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
    Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mapping of transcription start sites in Saccharomyces cerevisiae using 5' SAGE."
    Zhang Z., Dietrich F.S.
    Nucleic Acids Res. 33:2838-2851(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-97.
    Strain: ATCC 208353 / W303-1A.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHEM3_YEAST
AccessioniPrimary (citable) accession number: P28789
Secondary accession number(s): D6VRE9, Q2VQW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

The porphobilinogen subunits are added to the dipyrromethane group.By similarity
Present with 8480 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

External Data

Dasty 3