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Protein

Chorismate synthase

Gene

ARO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.

Cofactori

Pathwayi: chorismate biosynthesis

This protein is involved in step 7 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. Phospho-2-dehydro-3-deoxyheptonate aldolase, tyrosine-inhibited (ARO4), Phospho-2-dehydro-3-deoxyheptonate aldolase, phenylalanine-inhibited (ARO3)
  2. Pentafunctional AROM polypeptide (ARO1)
  3. Pentafunctional AROM polypeptide (ARO1)
  4. Pentafunctional AROM polypeptide (ARO1)
  5. Pentafunctional AROM polypeptide (ARO1)
  6. Pentafunctional AROM polypeptide (ARO1)
  7. Chorismate synthase (ARO2)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

GO - Molecular functioni

  • chorismate synthase activity Source: SGD
  • FMN binding Source: GO_Central
  • riboflavin reductase (NADPH) activity Source: SGD

GO - Biological processi

  • aromatic amino acid family biosynthetic process Source: SGD
  • cellular amino acid biosynthetic process Source: UniProtKB-KW
  • chorismate biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Stress response

Enzyme and pathway databases

BioCyciYEAST:YGL148W-MONOMER.
BRENDAi4.2.3.5. 984.
UniPathwayiUPA00053; UER00090.

Names & Taxonomyi

Protein namesi
Recommended name:
Chorismate synthase (EC:4.2.3.5)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate phospholyase
Gene namesi
Name:ARO2
Ordered Locus Names:YGL148W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL148W.
SGDiS000003116. ARO2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • cytosol Source: GO_Central
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001407042 – 376Chorismate synthaseAdd BLAST375

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP28777.
PRIDEiP28777.

PTM databases

iPTMnetiP28777.

Expressioni

Inductioni

By amino acid starvation.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi33104. 153 interactors.
DIPiDIP-4219N.
IntActiP28777. 6 interactors.
MINTiMINT-554974.

Structurei

Secondary structure

1376
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 13Combined sources8
Beta strandi18 – 26Combined sources9
Beta strandi30 – 33Combined sources4
Helixi36 – 38Combined sources3
Helixi40 – 44Combined sources5
Beta strandi63 – 65Combined sources3
Beta strandi67 – 70Combined sources4
Beta strandi79 – 84Combined sources6
Helixi130 – 145Combined sources16
Beta strandi150 – 158Combined sources9
Helixi170 – 178Combined sources9
Helixi181 – 186Combined sources6
Helixi195 – 210Combined sources16
Beta strandi217 – 225Combined sources9
Turni233 – 235Combined sources3
Helixi238 – 247Combined sources10
Beta strandi254 – 257Combined sources4
Turni258 – 261Combined sources4
Helixi262 – 265Combined sources4
Helixi268 – 272Combined sources5
Beta strandi293 – 295Combined sources3
Beta strandi304 – 310Combined sources7
Helixi342 – 345Combined sources4
Helixi347 – 370Combined sources24

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R52X-ray2.89A/B/C/D1-376[»]
1R53X-ray2.20A1-376[»]
ProteinModelPortaliP28777.
SMRiP28777.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28777.

Family & Domainsi

Sequence similaritiesi

Belongs to the chorismate synthase family.Curated

Phylogenomic databases

HOGENOMiHOG000060335.
InParanoidiP28777.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG092C2FBX.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28777-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTFGKLFRV TTYGESHCKS VGCIVDGVPP GMSLTEADIQ PQLTRRRPGQ
60 70 80 90 100
SKLSTPRDEK DRVEIQSGTE FGKTLGTPIA MMIKNEDQRP HDYSDMDKFP
110 120 130 140 150
RPSHADFTYS EKYGIKASSG GGRASARETI GRVASGAIAE KFLAQNSNVE
160 170 180 190 200
IVAFVTQIGE IKMNRDSFDP EFQHLLNTIT REKVDSMGPI RCPDASVAGL
210 220 230 240 250
MVKEIEKYRG NKDSIGGVVT CVVRNLPTGL GEPCFDKLEA MLAHAMLSIP
260 270 280 290 300
ASKGFEIGSG FQGVSVPGSK HNDPFYFEKE TNRLRTKTNN SGGVQGGISN
310 320 330 340 350
GENIYFSVPF KSVATISQEQ KTATYDGEEG ILAAKGRHDP AVTPRAIPIV
360 370
EAMTALVLAD ALLIQKARDF SRSVVH
Length:376
Mass (Da):40,838
Last modified:December 1, 1992 - v1
Checksum:iAF3AF65605B91E8E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60190 Genomic DNA. Translation: CAA42745.1.
X99960 Genomic DNA. Translation: CAA68214.1.
Z72670 Genomic DNA. Translation: CAA96860.1.
BK006941 Genomic DNA. Translation: DAA07962.1.
PIRiS17246.
RefSeqiNP_011367.1. NM_001181013.1.

Genome annotation databases

EnsemblFungiiYGL148W; YGL148W; YGL148W.
GeneIDi852729.
KEGGisce:YGL148W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60190 Genomic DNA. Translation: CAA42745.1.
X99960 Genomic DNA. Translation: CAA68214.1.
Z72670 Genomic DNA. Translation: CAA96860.1.
BK006941 Genomic DNA. Translation: DAA07962.1.
PIRiS17246.
RefSeqiNP_011367.1. NM_001181013.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1R52X-ray2.89A/B/C/D1-376[»]
1R53X-ray2.20A1-376[»]
ProteinModelPortaliP28777.
SMRiP28777.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33104. 153 interactors.
DIPiDIP-4219N.
IntActiP28777. 6 interactors.
MINTiMINT-554974.

PTM databases

iPTMnetiP28777.

Proteomic databases

MaxQBiP28777.
PRIDEiP28777.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL148W; YGL148W; YGL148W.
GeneIDi852729.
KEGGisce:YGL148W.

Organism-specific databases

EuPathDBiFungiDB:YGL148W.
SGDiS000003116. ARO2.

Phylogenomic databases

HOGENOMiHOG000060335.
InParanoidiP28777.
KOiK01736.
OMAiMLSINAV.
OrthoDBiEOG092C2FBX.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00090.
BioCyciYEAST:YGL148W-MONOMER.
BRENDAi4.2.3.5. 984.

Miscellaneous databases

EvolutionaryTraceiP28777.
PROiP28777.

Family and domain databases

CDDicd07304. Chorismate_synthase. 1 hit.
Gene3Di3.60.150.10. 1 hit.
HAMAPiMF_00300. Chorismate_synth. 1 hit.
InterProiIPR000453. Chorismate_synth.
IPR020541. Chorismate_synthase_CS.
[Graphical view]
PANTHERiPTHR21085. PTHR21085. 1 hit.
PfamiPF01264. Chorismate_synt. 1 hit.
[Graphical view]
PIRSFiPIRSF001456. Chorismate_synth. 1 hit.
SUPFAMiSSF103263. SSF103263. 1 hit.
TIGRFAMsiTIGR00033. aroC. 1 hit.
PROSITEiPS00787. CHORISMATE_SYNTHASE_1. 1 hit.
PS00788. CHORISMATE_SYNTHASE_2. 1 hit.
PS00789. CHORISMATE_SYNTHASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAROC_YEAST
AccessioniPrimary (citable) accession number: P28777
Secondary accession number(s): D6VU01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 2, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2310 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.