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P28776

- I23O1_MOUSE

UniProt

P28776 - I23O1_MOUSE

Protein

Indoleamine 2,3-dioxygenase 1

Gene

Ido1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.By similarity

    Catalytic activityi

    D-tryptophan + O2 = N-formyl-D-kynurenine.
    L-tryptophan + O2 = N-formyl-L-kynurenine.

    Cofactori

    Binds 1 heme group per subunit.

    Enzyme regulationi

    Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan).By similarity

    Kineticsi

    Catalytic efficiency for L-tryptophan is 90 times higher than for D-tryptophan.

    1. KM=28.1 µM for L-tryptophan1 Publication
    2. KM=2.87 mM for D-tryptophan1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi350 – 3501Iron (heme proximal ligand)By similarity

    GO - Molecular functioni

    1. heme binding Source: InterPro
    2. indoleamine 2,3-dioxygenase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. protein binding Source: IntAct
    5. tryptophan 2,3-dioxygenase activity Source: MGI

    GO - Biological processi

    1. cytokine production involved in inflammatory response Source: MGI
    2. defense response Source: MGI
    3. inflammatory response Source: MGI
    4. kynurenic acid biosynthetic process Source: MGI
    5. multicellular organismal response to stress Source: MGI
    6. negative regulation of activated T cell proliferation Source: MGI
    7. negative regulation of interleukin-10 production Source: MGI
    8. negative regulation of T cell apoptotic process Source: MGI
    9. negative regulation of T cell proliferation Source: MGI
    10. positive regulation of apoptotic process Source: MGI
    11. positive regulation of chronic inflammatory response Source: MGI
    12. positive regulation of interleukin-12 production Source: MGI
    13. positive regulation of T cell tolerance induction Source: MGI
    14. positive regulation of type 2 immune response Source: MGI
    15. response to lipopolysaccharide Source: MGI
    16. swimming behavior Source: MGI
    17. tryptophan catabolic process to kynurenine Source: MGI

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Tryptophan catabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.13.11.11. 3474.
    SABIO-RKP28776.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indoleamine 2,3-dioxygenase 1 (EC:1.13.11.52)
    Short name:
    IDO-1
    Alternative name(s):
    Indoleamine-pyrrole 2,3-dioxygenase
    Gene namesi
    Name:Ido1
    Synonyms:Ido, Indo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:96416. Ido1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. smooth muscle contractile fiber Source: MGI
    3. stereocilium bundle Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Indoleamine 2,3-dioxygenase 1PRO_0000215205Add
    BLAST

    Proteomic databases

    MaxQBiP28776.
    PRIDEiP28776.

    PTM databases

    PhosphoSiteiP28776.

    Expressioni

    Tissue specificityi

    Detected in the apical cells of the caput epididymis and in the kidney blood vessels (at protein level).1 Publication

    Inductioni

    By interferon gamma.

    Gene expression databases

    ArrayExpressiP28776.
    BgeeiP28776.
    CleanExiMM_INDO.
    GenevestigatoriP28776.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ptpn11P352355EBI-4410822,EBI-397236

    Protein-protein interaction databases

    BioGridi200512. 2 interactions.
    DIPiDIP-48663N.
    IntActiP28776. 2 interactions.
    STRINGi10090.ENSMUSP00000033956.

    Structurei

    3D structure databases

    ProteinModelPortaliP28776.
    SMRiP28776. Positions 15-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the indoleamine 2,3-dioxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG73554.
    GeneTreeiENSGT00390000002154.
    HOGENOMiHOG000190192.
    InParanoidiP28776.
    KOiK00463.
    OMAiNGANINM.
    PhylomeDBiP28776.
    TreeFamiTF330978.

    Family and domain databases

    InterProiIPR000898. Indolamine_dOase.
    [Graphical view]
    PfamiPF01231. IDO. 1 hit.
    [Graphical view]
    PROSITEiPS00876. IDO_1. 1 hit.
    PS00877. IDO_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28776-1 [UniParc]FASTAAdd to Basket

    « Hide

    MALSKISPTE GSRRILEDHH IDEDVGFALP HPLVELPDAY SPWVLVARNL    50
    PVLIENGQLR EEVEKLPTLS TDGLRGHRLQ RLAHLALGYI TMAYVWNRGD 100
    DDVRKVLPRN IAVPYCELSE KLGLPPILSY ADCVLANWKK KDPNGPMTYE 150
    NMDILFSFPG GDCDKGFFLV SLLVEIAASP AIKAIPTVSS AVERQDLKAL 200
    EKALHDIATS LEKAKEIFKR MRDFVDPDTF FHVLRIYLSG WKCSSKLPEG 250
    LLYEGVWDTP KMFSGGSAGQ SSIFQSLDVL LGIKHEAGKE SPAEFLQEMR 300
    EYMPPAHRNF LFFLESAPPV REFVISRHNE DLTKAYNECV NGLVSVRKFH 350
    LAIVDTYIMK PSKKKPTDGD KSEEPSNVES RGTGGTNPMT FLRSVKDTTE 400
    KALLSWP 407
    Length:407
    Mass (Da):45,641
    Last modified:December 1, 1992 - v1
    Checksum:i317681EABF96A727
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69109 mRNA. Translation: AAA37872.1.
    BC049931 mRNA. Translation: AAH49931.1.
    CCDSiCCDS40299.1.
    PIRiJH0492.
    RefSeqiNP_032350.1. NM_008324.2.
    UniGeneiMm.392.

    Genome annotation databases

    EnsembliENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
    GeneIDi15930.
    KEGGimmu:15930.
    UCSCiuc009lfa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M69109 mRNA. Translation: AAA37872.1 .
    BC049931 mRNA. Translation: AAH49931.1 .
    CCDSi CCDS40299.1.
    PIRi JH0492.
    RefSeqi NP_032350.1. NM_008324.2.
    UniGenei Mm.392.

    3D structure databases

    ProteinModelPortali P28776.
    SMRi P28776. Positions 15-404.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200512. 2 interactions.
    DIPi DIP-48663N.
    IntActi P28776. 2 interactions.
    STRINGi 10090.ENSMUSP00000033956.

    Chemistry

    ChEMBLi CHEMBL1075294.

    PTM databases

    PhosphoSitei P28776.

    Proteomic databases

    MaxQBi P28776.
    PRIDEi P28776.

    Protocols and materials databases

    DNASUi 15930.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033956 ; ENSMUSP00000033956 ; ENSMUSG00000031551 .
    GeneIDi 15930.
    KEGGi mmu:15930.
    UCSCi uc009lfa.1. mouse.

    Organism-specific databases

    CTDi 3620.
    MGIi MGI:96416. Ido1.

    Phylogenomic databases

    eggNOGi NOG73554.
    GeneTreei ENSGT00390000002154.
    HOGENOMi HOG000190192.
    InParanoidi P28776.
    KOi K00463.
    OMAi NGANINM.
    PhylomeDBi P28776.
    TreeFami TF330978.

    Enzyme and pathway databases

    BRENDAi 1.13.11.11. 3474.
    SABIO-RK P28776.

    Miscellaneous databases

    NextBioi 288648.
    PROi P28776.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P28776.
    Bgeei P28776.
    CleanExi MM_INDO.
    Genevestigatori P28776.

    Family and domain databases

    InterProi IPR000898. Indolamine_dOase.
    [Graphical view ]
    Pfami PF01231. IDO. 1 hit.
    [Graphical view ]
    PROSITEi PS00876. IDO_1. 1 hit.
    PS00877. IDO_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and expression of a cDNA encoding mouse indoleamine 2,3-dioxygenase."
      Habara-Ohkubo A., Takikawa O., Yoshida R.
      Gene 105:221-227(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary gland.
    3. "Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice."
      Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H.
      Gene 396:203-213(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiI23O1_MOUSE
    AccessioniPrimary (citable) accession number: P28776
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3