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Protein

Indoleamine 2,3-dioxygenase 1

Gene

Ido1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.By similarity

Catalytic activityi

D-tryptophan + O2 = N-formyl-D-kynurenine.
L-tryptophan + O2 = N-formyl-L-kynurenine.

Cofactori

hemeNote: Binds 1 heme group per subunit.

Enzyme regulationi

Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan).By similarity

Kineticsi

Catalytic efficiency for L-tryptophan is 90 times higher than for D-tryptophan.

  1. KM=28.1 µM for L-tryptophan1 Publication
  2. KM=2.87 mM for D-tryptophan1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi350 – 3501Iron (heme proximal ligand)By similarity

GO - Molecular functioni

  1. heme binding Source: InterPro
  2. indoleamine 2,3-dioxygenase activity Source: MGI
  3. metal ion binding Source: UniProtKB-KW
  4. tryptophan 2,3-dioxygenase activity Source: MGI

GO - Biological processi

  1. cytokine production involved in inflammatory response Source: MGI
  2. defense response Source: MGI
  3. inflammatory response Source: MGI
  4. kynurenic acid biosynthetic process Source: MGI
  5. multicellular organismal response to stress Source: MGI
  6. negative regulation of activated T cell proliferation Source: MGI
  7. negative regulation of interleukin-10 production Source: MGI
  8. negative regulation of T cell apoptotic process Source: MGI
  9. negative regulation of T cell proliferation Source: MGI
  10. positive regulation of apoptotic process Source: MGI
  11. positive regulation of chronic inflammatory response Source: MGI
  12. positive regulation of interleukin-12 production Source: MGI
  13. positive regulation of T cell apoptotic process Source: MGI
  14. positive regulation of T cell tolerance induction Source: MGI
  15. positive regulation of type 2 immune response Source: MGI
  16. response to lipopolysaccharide Source: MGI
  17. swimming behavior Source: MGI
  18. tryptophan catabolic process to kynurenine Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Tryptophan catabolism

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.13.11.11. 3474.
1.13.11.52. 3474.
ReactomeiREACT_340310. Tryptophan catabolism.
SABIO-RKP28776.

Names & Taxonomyi

Protein namesi
Recommended name:
Indoleamine 2,3-dioxygenase 1 (EC:1.13.11.52)
Short name:
IDO-1
Alternative name(s):
Indoleamine-pyrrole 2,3-dioxygenase
Gene namesi
Name:Ido1
Synonyms:Ido, Indo
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96416. Ido1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. smooth muscle contractile fiber Source: MGI
  3. stereocilium bundle Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 407407Indoleamine 2,3-dioxygenase 1PRO_0000215205Add
BLAST

Proteomic databases

MaxQBiP28776.
PRIDEiP28776.

PTM databases

PhosphoSiteiP28776.

Expressioni

Tissue specificityi

Detected in the apical cells of the caput epididymis and in the kidney blood vessels (at protein level).1 Publication

Inductioni

By interferon gamma.

Gene expression databases

BgeeiP28776.
CleanExiMM_INDO.
ExpressionAtlasiP28776. baseline and differential.
GenevestigatoriP28776.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
Ptpn11P352355EBI-4410822,EBI-397236

Protein-protein interaction databases

BioGridi200512. 2 interactions.
DIPiDIP-48663N.
IntActiP28776. 2 interactions.
STRINGi10090.ENSMUSP00000033956.

Structurei

3D structure databases

ProteinModelPortaliP28776.
SMRiP28776. Positions 15-404.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the indoleamine 2,3-dioxygenase family.Curated

Phylogenomic databases

eggNOGiNOG73554.
GeneTreeiENSGT00390000002154.
HOGENOMiHOG000190192.
InParanoidiP28776.
KOiK00463.
OMAiECVEAMV.
PhylomeDBiP28776.
TreeFamiTF330978.

Family and domain databases

InterProiIPR000898. Indolamine_dOase.
[Graphical view]
PfamiPF01231. IDO. 1 hit.
[Graphical view]
PROSITEiPS00876. IDO_1. 1 hit.
PS00877. IDO_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28776-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALSKISPTE GSRRILEDHH IDEDVGFALP HPLVELPDAY SPWVLVARNL
60 70 80 90 100
PVLIENGQLR EEVEKLPTLS TDGLRGHRLQ RLAHLALGYI TMAYVWNRGD
110 120 130 140 150
DDVRKVLPRN IAVPYCELSE KLGLPPILSY ADCVLANWKK KDPNGPMTYE
160 170 180 190 200
NMDILFSFPG GDCDKGFFLV SLLVEIAASP AIKAIPTVSS AVERQDLKAL
210 220 230 240 250
EKALHDIATS LEKAKEIFKR MRDFVDPDTF FHVLRIYLSG WKCSSKLPEG
260 270 280 290 300
LLYEGVWDTP KMFSGGSAGQ SSIFQSLDVL LGIKHEAGKE SPAEFLQEMR
310 320 330 340 350
EYMPPAHRNF LFFLESAPPV REFVISRHNE DLTKAYNECV NGLVSVRKFH
360 370 380 390 400
LAIVDTYIMK PSKKKPTDGD KSEEPSNVES RGTGGTNPMT FLRSVKDTTE

KALLSWP
Length:407
Mass (Da):45,641
Last modified:December 1, 1992 - v1
Checksum:i317681EABF96A727
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69109 mRNA. Translation: AAA37872.1.
BC049931 mRNA. Translation: AAH49931.1.
CCDSiCCDS40299.1.
PIRiJH0492.
RefSeqiNP_001280619.1. NM_001293690.1.
NP_032350.1. NM_008324.2.
UniGeneiMm.392.

Genome annotation databases

EnsembliENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
GeneIDi15930.
KEGGimmu:15930.
UCSCiuc009lfa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M69109 mRNA. Translation: AAA37872.1.
BC049931 mRNA. Translation: AAH49931.1.
CCDSiCCDS40299.1.
PIRiJH0492.
RefSeqiNP_001280619.1. NM_001293690.1.
NP_032350.1. NM_008324.2.
UniGeneiMm.392.

3D structure databases

ProteinModelPortaliP28776.
SMRiP28776. Positions 15-404.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200512. 2 interactions.
DIPiDIP-48663N.
IntActiP28776. 2 interactions.
STRINGi10090.ENSMUSP00000033956.

Chemistry

BindingDBiP28776.
ChEMBLiCHEMBL1075294.

PTM databases

PhosphoSiteiP28776.

Proteomic databases

MaxQBiP28776.
PRIDEiP28776.

Protocols and materials databases

DNASUi15930.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
GeneIDi15930.
KEGGimmu:15930.
UCSCiuc009lfa.1. mouse.

Organism-specific databases

CTDi3620.
MGIiMGI:96416. Ido1.

Phylogenomic databases

eggNOGiNOG73554.
GeneTreeiENSGT00390000002154.
HOGENOMiHOG000190192.
InParanoidiP28776.
KOiK00463.
OMAiECVEAMV.
PhylomeDBiP28776.
TreeFamiTF330978.

Enzyme and pathway databases

BRENDAi1.13.11.11. 3474.
1.13.11.52. 3474.
ReactomeiREACT_340310. Tryptophan catabolism.
SABIO-RKP28776.

Miscellaneous databases

NextBioi288648.
PROiP28776.
SOURCEiSearch...

Gene expression databases

BgeeiP28776.
CleanExiMM_INDO.
ExpressionAtlasiP28776. baseline and differential.
GenevestigatoriP28776.

Family and domain databases

InterProiIPR000898. Indolamine_dOase.
[Graphical view]
PfamiPF01231. IDO. 1 hit.
[Graphical view]
PROSITEiPS00876. IDO_1. 1 hit.
PS00877. IDO_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and expression of a cDNA encoding mouse indoleamine 2,3-dioxygenase."
    Habara-Ohkubo A., Takikawa O., Yoshida R.
    Gene 105:221-227(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NMRI.
    Tissue: Mammary gland.
  3. "Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice."
    Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H.
    Gene 396:203-213(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  4. Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

Entry informationi

Entry nameiI23O1_MOUSE
AccessioniPrimary (citable) accession number: P28776
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 1, 2015
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.