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P28776 (I23O1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Indoleamine 2,3-dioxygenase 1

Short name=IDO-1
EC=1.13.11.52
Alternative name(s):
Indoleamine-pyrrole 2,3-dioxygenase
Gene names
Name:Ido1
Synonyms:Ido, Indo
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen By similarity.

Catalytic activity

D-tryptophan + O2 = N-formyl-D-kynurenine.

L-tryptophan + O2 = N-formyl-L-kynurenine.

Cofactor

Binds 1 heme group per subunit.

Enzyme regulation

Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan) By similarity.

Tissue specificity

Detected in the apical cells of the caput epididymis and in the kidney blood vessels (at protein level). Ref.3

Induction

By interferon gamma.

Sequence similarities

Belongs to the indoleamine 2,3-dioxygenase family.

Biophysicochemical properties

Kinetic parameters:

Catalytic efficiency for L-tryptophan is 90 times higher than for D-tryptophan.

KM=28.1 µM for L-tryptophan Ref.4

KM=2.87 mM for D-tryptophan

Ontologies

Keywords
   Biological processTryptophan catabolism
   LigandHeme
Iron
Metal-binding
   Molecular functionDioxygenase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcytokine production involved in inflammatory response

Inferred from mutant phenotype PubMed 19234212. Source: MGI

defense response

Traceable author statement PubMed 2419335. Source: MGI

inflammatory response

Inferred from mutant phenotype PubMed 18480171. Source: MGI

kynurenic acid biosynthetic process

Inferred from mutant phenotype PubMed 19234212. Source: MGI

multicellular organismal response to stress

Inferred from mutant phenotype PubMed 19234218. Source: MGI

negative regulation of T cell apoptotic process

Inferred from mutant phenotype PubMed 18077788. Source: MGI

negative regulation of T cell proliferation

Inferred from mutant phenotype PubMed 18077788. Source: MGI

negative regulation of activated T cell proliferation

Inferred from mutant phenotype PubMed 18077788. Source: MGI

negative regulation of interleukin-10 production

Inferred from mutant phenotype PubMed 19234212. Source: MGI

positive regulation of T cell tolerance induction

Inferred from mutant phenotype PubMed 9712583. Source: MGI

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 18480171. Source: MGI

positive regulation of chronic inflammatory response

Inferred from mutant phenotype PubMed 18436652. Source: MGI

positive regulation of interleukin-12 production

Inferred from mutant phenotype PubMed 19234212. Source: MGI

positive regulation of type 2 immune response

Inferred from mutant phenotype PubMed 18436652. Source: MGI

response to lipopolysaccharide

Inferred from mutant phenotype PubMed 19234212. Source: MGI

swimming behavior

Inferred from mutant phenotype PubMed 19234218. Source: MGI

tryptophan catabolic process to kynurenine

Inferred from direct assay PubMed 11751753PubMed 2419335. Source: MGI

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17671174PubMed 19741271. Source: MGI

smooth muscle contractile fiber

Inferred from direct assay PubMed 19741271. Source: MGI

stereocilium bundle

Inferred from direct assay PubMed 19741271. Source: MGI

   Molecular_functionheme binding

Inferred from electronic annotation. Source: InterPro

indoleamine 2,3-dioxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

tryptophan 2,3-dioxygenase activity

Inferred from direct assay PubMed 11751753. Source: MGI

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ptpn11P352355EBI-4410822,EBI-397236

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Indoleamine 2,3-dioxygenase 1
PRO_0000215205

Sites

Metal binding3501Iron (heme proximal ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P28776 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 317681EABF96A727

FASTA40745,641
        10         20         30         40         50         60 
MALSKISPTE GSRRILEDHH IDEDVGFALP HPLVELPDAY SPWVLVARNL PVLIENGQLR 

        70         80         90        100        110        120 
EEVEKLPTLS TDGLRGHRLQ RLAHLALGYI TMAYVWNRGD DDVRKVLPRN IAVPYCELSE 

       130        140        150        160        170        180 
KLGLPPILSY ADCVLANWKK KDPNGPMTYE NMDILFSFPG GDCDKGFFLV SLLVEIAASP 

       190        200        210        220        230        240 
AIKAIPTVSS AVERQDLKAL EKALHDIATS LEKAKEIFKR MRDFVDPDTF FHVLRIYLSG 

       250        260        270        280        290        300 
WKCSSKLPEG LLYEGVWDTP KMFSGGSAGQ SSIFQSLDVL LGIKHEAGKE SPAEFLQEMR 

       310        320        330        340        350        360 
EYMPPAHRNF LFFLESAPPV REFVISRHNE DLTKAYNECV NGLVSVRKFH LAIVDTYIMK 

       370        380        390        400 
PSKKKPTDGD KSEEPSNVES RGTGGTNPMT FLRSVKDTTE KALLSWP 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and expression of a cDNA encoding mouse indoleamine 2,3-dioxygenase."
Habara-Ohkubo A., Takikawa O., Yoshida R.
Gene 105:221-227(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary gland.
[3]"Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice."
Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H.
Gene 396:203-213(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[4]"Evolution of vertebrate indoleamine 2,3-dioxygenases."
Yuasa H.J., Takubo M., Takahashi A., Hasegawa T., Noma H., Suzuki T.
J. Mol. Evol. 65:705-714(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: BIOPHYSICOCHEMICAL PROPERTIES.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M69109 mRNA. Translation: AAA37872.1.
BC049931 mRNA. Translation: AAH49931.1.
PIRJH0492.
RefSeqNP_032350.1. NM_008324.1.
UniGeneMm.392.

3D structure databases

ProteinModelPortalP28776.
SMRP28776. Positions 15-404.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200512. 2 interactions.
DIPDIP-48663N.
IntActP28776. 2 interactions.
STRING10090.ENSMUSP00000033956.

Chemistry

ChEMBLCHEMBL1075294.

PTM databases

PhosphoSiteP28776.

Proteomic databases

PRIDEP28776.

Protocols and materials databases

DNASU15930.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
GeneID15930.
KEGGmmu:15930.
UCSCuc009lfa.1. mouse.

Organism-specific databases

CTD3620.
MGIMGI:96416. Ido1.

Phylogenomic databases

eggNOGNOG73554.
GeneTreeENSGT00390000002154.
HOGENOMHOG000190192.
InParanoidP28776.
KOK00463.
OMAQIVAKYI.
PhylomeDBP28776.
TreeFamTF330978.

Enzyme and pathway databases

BRENDA1.13.11.11. 3474.
SABIO-RKP28776.

Gene expression databases

ArrayExpressP28776.
BgeeP28776.
CleanExMM_INDO.
GenevestigatorP28776.

Family and domain databases

InterProIPR000898. Indolamine_dOase.
[Graphical view]
PfamPF01231. IDO. 1 hit.
[Graphical view]
PROSITEPS00876. IDO_1. 1 hit.
PS00877. IDO_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288648.
PROP28776.
SOURCESearch...

Entry information

Entry nameI23O1_MOUSE
AccessionPrimary (citable) accession number: P28776
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot