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Protein

Indoleamine 2,3-dioxygenase 1

Gene

Ido1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen.By similarity

Catalytic activityi

D-tryptophan + O2 = N-formyl-D-kynurenine.
L-tryptophan + O2 = N-formyl-L-kynurenine.

Cofactori

hemeNote: Binds 1 heme group per subunit.

Enzyme regulationi

Activity is inhibited by and MTH-trp (methylthiohydantoin-DL-tryptophan), modestly inhibited by L-1MT (1-methyl-L-tryptophan) but not D-1MT (1-methyl-D-tryptophan).By similarity

Kineticsi

Catalytic efficiency for L-tryptophan is 90 times higher than for D-tryptophan.

  1. KM=28.1 µM for L-tryptophan1 Publication
  2. KM=2.87 mM for D-tryptophan1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi350 – 3501Iron (heme proximal ligand)By similarity

    GO - Molecular functioni

    GO - Biological processi

    • cytokine production involved in inflammatory response Source: MGI
    • defense response Source: MGI
    • inflammatory response Source: MGI
    • kynurenic acid biosynthetic process Source: MGI
    • multicellular organismal response to stress Source: MGI
    • negative regulation of activated T cell proliferation Source: MGI
    • negative regulation of interleukin-10 production Source: MGI
    • negative regulation of T cell apoptotic process Source: MGI
    • negative regulation of T cell proliferation Source: MGI
    • positive regulation of apoptotic process Source: MGI
    • positive regulation of chronic inflammatory response Source: MGI
    • positive regulation of interleukin-12 production Source: MGI
    • positive regulation of T cell apoptotic process Source: MGI
    • positive regulation of T cell tolerance induction Source: MGI
    • positive regulation of type 2 immune response Source: MGI
    • response to lipopolysaccharide Source: MGI
    • swimming behavior Source: MGI
    • tryptophan catabolic process to kynurenine Source: MGI
    Complete GO annotation...

    Keywords - Molecular functioni

    Dioxygenase, Oxidoreductase

    Keywords - Biological processi

    Tryptophan catabolism

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BRENDAi1.13.11.11. 3474.
    1.13.11.52. 3474.
    ReactomeiREACT_340310. Tryptophan catabolism.
    SABIO-RKP28776.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indoleamine 2,3-dioxygenase 1 (EC:1.13.11.52)
    Short name:
    IDO-1
    Alternative name(s):
    Indoleamine-pyrrole 2,3-dioxygenase
    Gene namesi
    Name:Ido1
    Synonyms:Ido, Indo
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589 Componenti: Chromosome 8

    Organism-specific databases

    MGIiMGI:96416. Ido1.

    Subcellular locationi

    GO - Cellular componenti

    • cytoplasm Source: MGI
    • smooth muscle contractile fiber Source: MGI
    • stereocilium bundle Source: MGI
    Complete GO annotation...

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 407407Indoleamine 2,3-dioxygenase 1PRO_0000215205Add
    BLAST

    Proteomic databases

    MaxQBiP28776.
    PRIDEiP28776.

    PTM databases

    PhosphoSiteiP28776.

    Expressioni

    Tissue specificityi

    Detected in the apical cells of the caput epididymis and in the kidney blood vessels (at protein level).1 Publication

    Inductioni

    By interferon gamma.

    Gene expression databases

    BgeeiP28776.
    CleanExiMM_INDO.
    ExpressionAtlasiP28776. baseline and differential.
    GenevestigatoriP28776.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ptpn11P352355EBI-4410822,EBI-397236

    Protein-protein interaction databases

    BioGridi200512. 2 interactions.
    DIPiDIP-48663N.
    IntActiP28776. 2 interactions.
    STRINGi10090.ENSMUSP00000033956.

    Structurei

    3D structure databases

    ProteinModelPortaliP28776.
    SMRiP28776. Positions 15-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the indoleamine 2,3-dioxygenase family.Curated

    Phylogenomic databases

    eggNOGiNOG73554.
    GeneTreeiENSGT00390000002154.
    HOGENOMiHOG000190192.
    InParanoidiP28776.
    KOiK00463.
    OMAiECVEAMV.
    PhylomeDBiP28776.
    TreeFamiTF330978.

    Family and domain databases

    InterProiIPR000898. Indolamine_dOase.
    [Graphical view]
    PfamiPF01231. IDO. 1 hit.
    [Graphical view]
    PROSITEiPS00876. IDO_1. 1 hit.
    PS00877. IDO_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28776-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MALSKISPTE GSRRILEDHH IDEDVGFALP HPLVELPDAY SPWVLVARNL
    60 70 80 90 100
    PVLIENGQLR EEVEKLPTLS TDGLRGHRLQ RLAHLALGYI TMAYVWNRGD
    110 120 130 140 150
    DDVRKVLPRN IAVPYCELSE KLGLPPILSY ADCVLANWKK KDPNGPMTYE
    160 170 180 190 200
    NMDILFSFPG GDCDKGFFLV SLLVEIAASP AIKAIPTVSS AVERQDLKAL
    210 220 230 240 250
    EKALHDIATS LEKAKEIFKR MRDFVDPDTF FHVLRIYLSG WKCSSKLPEG
    260 270 280 290 300
    LLYEGVWDTP KMFSGGSAGQ SSIFQSLDVL LGIKHEAGKE SPAEFLQEMR
    310 320 330 340 350
    EYMPPAHRNF LFFLESAPPV REFVISRHNE DLTKAYNECV NGLVSVRKFH
    360 370 380 390 400
    LAIVDTYIMK PSKKKPTDGD KSEEPSNVES RGTGGTNPMT FLRSVKDTTE

    KALLSWP
    Length:407
    Mass (Da):45,641
    Last modified:December 1, 1992 - v1
    Checksum:i317681EABF96A727
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M69109 mRNA. Translation: AAA37872.1.
    BC049931 mRNA. Translation: AAH49931.1.
    CCDSiCCDS40299.1.
    PIRiJH0492.
    RefSeqiNP_001280619.1. NM_001293690.1.
    NP_032350.1. NM_008324.2.
    UniGeneiMm.392.

    Genome annotation databases

    EnsembliENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
    GeneIDi15930.
    KEGGimmu:15930.
    UCSCiuc009lfa.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    M69109 mRNA. Translation: AAA37872.1.
    BC049931 mRNA. Translation: AAH49931.1.
    CCDSiCCDS40299.1.
    PIRiJH0492.
    RefSeqiNP_001280619.1. NM_001293690.1.
    NP_032350.1. NM_008324.2.
    UniGeneiMm.392.

    3D structure databases

    ProteinModelPortaliP28776.
    SMRiP28776. Positions 15-404.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi200512. 2 interactions.
    DIPiDIP-48663N.
    IntActiP28776. 2 interactions.
    STRINGi10090.ENSMUSP00000033956.

    Chemistry

    BindingDBiP28776.
    ChEMBLiCHEMBL1075294.

    PTM databases

    PhosphoSiteiP28776.

    Proteomic databases

    MaxQBiP28776.
    PRIDEiP28776.

    Protocols and materials databases

    DNASUi15930.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000033956; ENSMUSP00000033956; ENSMUSG00000031551.
    GeneIDi15930.
    KEGGimmu:15930.
    UCSCiuc009lfa.1. mouse.

    Organism-specific databases

    CTDi3620.
    MGIiMGI:96416. Ido1.

    Phylogenomic databases

    eggNOGiNOG73554.
    GeneTreeiENSGT00390000002154.
    HOGENOMiHOG000190192.
    InParanoidiP28776.
    KOiK00463.
    OMAiECVEAMV.
    PhylomeDBiP28776.
    TreeFamiTF330978.

    Enzyme and pathway databases

    BRENDAi1.13.11.11. 3474.
    1.13.11.52. 3474.
    ReactomeiREACT_340310. Tryptophan catabolism.
    SABIO-RKP28776.

    Miscellaneous databases

    NextBioi288648.
    PROiP28776.
    SOURCEiSearch...

    Gene expression databases

    BgeeiP28776.
    CleanExiMM_INDO.
    ExpressionAtlasiP28776. baseline and differential.
    GenevestigatoriP28776.

    Family and domain databases

    InterProiIPR000898. Indolamine_dOase.
    [Graphical view]
    PfamiPF01231. IDO. 1 hit.
    [Graphical view]
    PROSITEiPS00876. IDO_1. 1 hit.
    PS00877. IDO_2. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and expression of a cDNA encoding mouse indoleamine 2,3-dioxygenase."
      Habara-Ohkubo A., Takikawa O., Yoshida R.
      Gene 105:221-227(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary gland.
    3. "Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice."
      Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H.
      Gene 396:203-213(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    4. Cited for: BIOPHYSICOCHEMICAL PROPERTIES.

    Entry informationi

    Entry nameiI23O1_MOUSE
    AccessioniPrimary (citable) accession number: P28776
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: April 1, 2015
    This is version 120 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.