Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P28774 (AT1B_ARTSF) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/potassium-transporting ATPase subunit alpha-B

Short name=Na(+)/K(+) ATPase alpha-B subunit
EC=3.6.3.9
Alternative name(s):
Sodium pump subunit alpha-B
OrganismArtemia franciscana (Brine shrimp) (Artemia sanfranciscana)
Taxonomic identifier6661 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaCrustaceaBranchiopodaAnostracaArtemiidaeArtemia

Protein attributes

Sequence length1004 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.

Catalytic activity

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Subunit structure

Composed of three subunits: alpha (catalytic), beta and gamma.

Subcellular location

Membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10041004Sodium/potassium-transporting ATPase subunit alpha-B
PRO_0000046307

Regions

Transmembrane76 – 9621Helical; By similarity
Transmembrane110 – 12617Helical; By similarity
Transmembrane272 – 29423Helical; By similarity
Transmembrane301 – 32929Helical; By similarity
Transmembrane768 – 79124Helical; By similarity
Transmembrane828 – 85528Helical; By similarity
Transmembrane897 – 91822Helical; By similarity
Transmembrane934 – 95926Helical; By similarity

Sites

Active site35714-aspartylphosphate intermediate Probable
Metal binding6981Magnesium By similarity
Metal binding7021Magnesium By similarity
Binding site4891ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P28774 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: CE4E6BECE19A78C7

FASTA1,004110,699
        10         20         30         40         50         60 
MAKGKQKKGK DLNELKKELD IDFHKIPIEE CYQRLGSNPE TGLTNAQARS NIERDGPNCL 

        70         80         90        100        110        120 
TPPKTTPEWI KFCKNLFGGF ALLLWTGAIL CFLAYGIEAS SGNEDMLKDN LYLGIVLATV 

       130        140        150        160        170        180 
VIVTGIFSYY QENKSSRIMD SFKNLVPQYA LALREGQRVT LKAEELTMGD IVEVKFGDRV 

       190        200        210        220        230        240 
PADLRVLEAR SFKVDNSSLT GESEPQARSP EFTNDNPLET KNLAFFSTNA VEGTMRGIVI 

       250        260        270        280        290        300 
GIGDNTVMGR IAGLASGLDT GETPIAKEIA HFIHIITGVA VFLGVTFFII AFVLGYHWLD 

       310        320        330        340        350        360 
AVVFLIGIIV ANVPEGLLAT VTVCLTLTAK RMASKNCLVK NLEAVETLGS TSTICSDKTG 

       370        380        390        400        410        420 
TLTQNRMTVA HMWFDGTITE ADTTEDQSGA QFDKSSAGWK ALVKIAALCS RAEFKPNQST 

       430        440        450        460        470        480 
TPILKREVTG DASEAAILKC VELTTGETEA IRKRNKKICE IPFNSANKFQ VSIHENEDKS 

       490        500        510        520        530        540 
DGRYLLVMKG APERILERCS TIFMNGKEID MTEELKEAFN NAYMELGGLG ERVLGFCDYL 

       550        560        570        580        590        600 
LPLDKYPHGF AFNADDANFP LTGLRFAGLM SMIDPPRAAV PDAVAKCRSA GIKVIMVTGD 

       610        620        630        640        650        660 
HPITAKAIAK SVGIISEGNE TVEDIAARLN IPVSEVNPRD AKAAVVHGGE LRDITPDALD 

       670        680        690        700        710        720 
EILRHHPEIV FARTSPQQKL IIVEGCQRQG AIVAVTGDGV NDSPALKKAD IGVAMGIAGS 

       730        740        750        760        770        780 
DVSKQAADMI LLDDNFASIV TGVEEGRLIF DNLKKSIVYT LTSNIPEISP FLLFILFDIP 

       790        800        810        820        830        840 
LPLGTVTILC IDLGTDMVPA ISLAYEEAES DIMKRRPRNP VTDKLVNERL ISLAYGQIGM 

       850        860        870        880        890        900 
IQASAGFFVY FVIMAECGFL PWDLFGLRKH WDSRAVNDLT DSYGQEWTYD ARKQLESSCH 

       910        920        930        940        950        960 
TAYFVSIVIV QWADLIISKT RRNSVFQQGM RNNILNFALV FETCLAAFLS YTPGMDKGLR 

       970        980        990       1000 
MYPLKINWWF PALPFSFLIF VYDEARKFIL RRNPGGWVEQ ETYY 

« Hide

References

[1]"Cloning of a cDNA encoding an Artemia franciscana Na/K ATPase alpha-subunit."
Macias M.T., Martinez J.L., Palmero I., Sastre L.
Gene 105:197-204(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56650 mRNA. Translation: CAA39972.1.
PIRJH0470.

3D structure databases

ProteinModelPortalP28774.
SMRP28774. Positions 7-1004.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP28774.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAT1B_ARTSF
AccessionPrimary (citable) accession number: P28774
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families