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Protein

GTP-binding nuclear protein spi1

Gene

spi1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-binding protein involved in nucleocytoplasmic transport. Required for the import of protein into the nucleus and also for RNA export.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67GTP; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 24GTPBy similarity8
Nucleotide bindingi121 – 124GTPBy similarity4
Nucleotide bindingi149 – 151GTPBy similarity3

GO - Molecular functioni

  • GTPase activity Source: PomBase
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • poly(A)+ mRNA export from nucleus Source: PomBase
  • protein import into nucleus Source: GO_Central
  • ribosomal subunit export from nucleus Source: GO_Central
  • signal transduction Source: InterPro

Keywordsi

Biological processProtein transport, Transport
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
GTP-binding nuclear protein spi1
Gene namesi
Name:spi1
ORF Names:SPBC1289.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC1289.03c.
PomBaseiSPBC1289.03c. spi1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002087351 – 216GTP-binding nuclear protein spi1Add BLAST216

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28748.
PRIDEiP28748.

PTM databases

iPTMnetiP28748.

Interactioni

Subunit structurei

Oligomer of dis3, pim1 and spi1. Found in a nuclear export complex with RanGTP, exportin and pre-miRNA (By similarity). Interacts with fft3.By similarity2 Publications

Protein-protein interaction databases

BioGridi276686. 18 interactors.
MINTiMINT-4688107.
STRINGi4896.SPBC1289.03c.1.

Structurei

3D structure databases

ProteinModelPortaliP28748.
SMRiP28748.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the small GTPase superfamily. Ran family.Curated

Phylogenomic databases

HOGENOMiHOG000216664.
InParanoidiP28748.
KOiK07936.
OMAiYNVWDTA.
OrthoDBiEOG092C4F6L.
PhylomeDBiP28748.

Family and domain databases

InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR002041. Ran_GTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
PfamiView protein in Pfam
PF00071. Ras. 1 hit.
PRINTSiPR00627. GTPRANTC4.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiView protein in PROSITE
PS51418. RAN. 1 hit.

Sequencei

Sequence statusi: Complete.

P28748-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPQNVPTF KLVLVGDGGT GKTTFVKRHL TGEFEKKYIA TLGVEVHPLH
60 70 80 90 100
FHTNFGEICF NVWDTAGQEK LGGLRDGYYI QGQCGIIMFD VTSRITYKNV
110 120 130 140 150
PHWWRDLVRV CENIPIVLCG NKVDVKERKV KAKAITFHRK KNLQYYDISA
160 170 180 190 200
KSNYNFEKPF LWLARKLVGN PNLEFVASPA LAPPEVQVDQ QLLAQYQQEM
210
NEAAAMPLPD EDDADL
Length:216
Mass (Da):24,555
Last modified:December 1, 1992 - v1
Checksum:i9349A9155E315442
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73527 Genomic DNA. No translation available.
CU329671 Genomic DNA. Translation: CAB38683.1.
PIRiA40039.
RefSeqiNP_596827.1. NM_001023848.2.

Genome annotation databases

EnsemblFungiiSPBC1289.03c.1; SPBC1289.03c.1:pep; SPBC1289.03c.
GeneIDi2540149.
KEGGispo:SPBC1289.03c.

Similar proteinsi

Entry informationi

Entry nameiSPI1_SCHPO
AccessioniPrimary (citable) accession number: P28748
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 27, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families