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Protein

Protein pim1

Gene

pim1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ran(spi1)-bound GDP by GTP. Involved in the control of mitosis. Regulates a variety of nuclear events, including mitotic check-point, chromosome decondensation and mRNA processing/transport.

GO - Molecular functioni

  • Ran guanyl-nucleotide exchange factor activity Source: PomBase

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  • positive regulation of GTPase activity Source: GOC
  • positive regulation of protein export from nucleus Source: PomBase
  • Ran protein signal transduction Source: PomBase
  • regulation of exit from mitosis Source: PomBase
  • regulation of mitotic spindle assembly Source: PomBase
  • regulation of mitotic spindle elongation Source: PomBase
  • small GTPase mediated signal transduction Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein pim1
Alternative name(s):
Poly(A)+ RNA transport protein 2
Gene namesi
Name:pim1
Synonyms:dcd1, ptr2
ORF Names:SPBC557.03c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC557.03c.
PomBaseiSPBC557.03c. pim1.

Subcellular locationi

GO - Cellular componenti

  • mitotic spindle midzone Source: PomBase
  • nuclear chromatin Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 539539Protein pim1PRO_0000206636Add
BLAST

Proteomic databases

MaxQBiP28745.

Interactioni

Subunit structurei

Oligomer of dis3, pim1 and spi1. Interacts with ned1.2 Publications

Protein-protein interaction databases

BioGridi277574. 12 interactions.
MINTiMINT-4688085.

Structurei

3D structure databases

ProteinModelPortaliP28745.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati70 – 12556RCC1 1Add
BLAST
Repeati127 – 19165RCC1 2Add
BLAST
Repeati192 – 24352RCC1 3Add
BLAST
Repeati244 – 29653RCC1 4Add
BLAST
Repeati298 – 35356RCC1 5Add
BLAST
Repeati354 – 41764RCC1 6Add
BLAST
Repeati419 – 47254RCC1 7Add
BLAST

Sequence similaritiesi

Contains 7 RCC1 repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000170207.
KOiK11493.
OMAiQHESIVF.
PhylomeDBiP28745.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 6 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 3 hits.
PS50012. RCC1_3. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSNRSTRSS TKREEVSKNG VEKRELDESD VMKNGKKPVK RAKVSSLPKP
60 70 80 90 100
VRVPGSAKRI NKIPELPTER LNVYVFGSGS MNELGMGEEE MDVVYRPRLN
110 120 130 140 150
PILSTDKVGV VDLAVGGMHS AALSHDGRVY TWGVNDDYAL GRLTKDQKDE
160 170 180 190 200
NGDKVDNDLL EGTPSKVEGA LSHLRVTKVI CSDNLTAAIT DNGCCFTWGT
210 220 230 240 250
FRCSDGVLGY SDSQKRTAEP TQMRLPEICQ LATGTDHIIA LTTTGKVYTW
260 270 280 290 300
GNGQQFQLGR RMLERRRLQG LTPQPLALKN IISVGAGSYH SFAIDNKGRV
310 320 330 340 350
YAWGLNITRQ CGIEVEDEEE GAVITKPTLV DALEGYNVKS ITGGEHHTLA
360 370 380 390 400
LLEDGRVLAW GRDDRHQLGI PDNALPETVV KDEKGNNYYL STPTIIPGLT
410 420 430 440 450
NVIQVVCGTH HNLAVTSDGK VYSWGSAENY EVGQGDNDED VAVPTLVRSK
460 470 480 490 500
AIKEVAIRVA GAGGQFSIIA GIPNASEEPV ANGIKSEPEN EKKLKTEETS
510 520 530
KTDDSPVTDA KPDVTSNGEP STATSESKDS VLEPSSTTA
Length:539
Mass (Da):58,349
Last modified:January 11, 2001 - v2
Checksum:i35C0AF47CF6581F3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241S → L (PubMed:1855255).Curated
Sequence conflicti526 – 5261E → D (PubMed:1855255).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73528 Genomic DNA. No translation available.
CU329671 Genomic DNA. Translation: CAB60670.1.
PIRiB40039.
T50368.
RefSeqiNP_596026.1. NM_001021935.2.

Genome annotation databases

EnsemblFungiiSPBC557.03c.1; SPBC557.03c.1:pep; SPBC557.03c.
GeneIDi2541059.
KEGGispo:SPBC557.03c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73528 Genomic DNA. No translation available.
CU329671 Genomic DNA. Translation: CAB60670.1.
PIRiB40039.
T50368.
RefSeqiNP_596026.1. NM_001021935.2.

3D structure databases

ProteinModelPortaliP28745.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277574. 12 interactions.
MINTiMINT-4688085.

Proteomic databases

MaxQBiP28745.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC557.03c.1; SPBC557.03c.1:pep; SPBC557.03c.
GeneIDi2541059.
KEGGispo:SPBC557.03c.

Organism-specific databases

EuPathDBiFungiDB:SPBC557.03c.
PomBaseiSPBC557.03c. pim1.

Phylogenomic databases

HOGENOMiHOG000170207.
KOiK11493.
OMAiQHESIVF.
PhylomeDBiP28745.

Miscellaneous databases

NextBioi20802173.
PROiP28745.

Family and domain databases

Gene3Di2.130.10.30. 1 hit.
InterProiIPR009091. RCC1/BLIP-II.
IPR000408. Reg_chr_condens.
[Graphical view]
PfamiPF00415. RCC1. 6 hits.
[Graphical view]
PRINTSiPR00633. RCCNDNSATION.
SUPFAMiSSF50985. SSF50985. 1 hit.
PROSITEiPS00625. RCC1_1. 1 hit.
PS00626. RCC1_2. 3 hits.
PS50012. RCC1_3. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Premature initiation of mitosis in yeast lacking RCC1 or an interacting GTPase."
    Matsumoto T., Beach D.H.
    Cell 66:347-360(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. "Dis3, implicated in mitotic control, binds directly to Ran and enhances the GEF activity of RCC1."
    Noguchi E., Hayashi N., Azuma Y., Seki T., Nakamura M., Nakashima N., Yanagida M., He X., Mueller U., Sazer S., Nishimoto T.
    EMBO J. 15:5595-5605(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DIS3 AND SPI1.
  4. "An evolutionarily conserved fission yeast protein, Ned1, implicated in normal nuclear morphology and chromosome stability, interacts with Dis3, Pim1/RCC1 and an essential nucleoporin."
    Tange Y., Hirata A., Niwa O.
    J. Cell Sci. 115:4375-4385(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NED1.

Entry informationi

Entry nameiRCC1_SCHPO
AccessioniPrimary (citable) accession number: P28745
Secondary accession number(s): Q9USS3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 11, 2001
Last modified: April 13, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.