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P28744 (PLY3_AMBAR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pectate lyase 3

EC=4.2.2.2
Alternative name(s):
Antigen Amb a I
Antigen E
Short name=AgE
Pollen allergen Amb a 1.4
Allergen=Amb a 1.4
OrganismAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifier4212 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Protein attributes

Sequence length392 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Has pectate lyase activity By similarity.

Catalytic activity

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactor

Binds 1 calcium ion By similarity.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 2/5.

Subunit structure

Monomer.

Tissue specificity

Pollen and flowers.

Post-translational modification

The N-terminus is blocked.

Allergenic properties

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.

Sequence similarities

Belongs to the polysaccharide lyase 1 family. Amb a subfamily.

Ontologies

Keywords
   Coding sequence diversityPolymorphism
   DiseaseAllergen
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionLyase
   PTMDisulfide bond
Glycoprotein
Gene Ontology (GO)
None. [Check GOA]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 392367Pectate lyase 3
PRO_0000024904

Sites

Active site2701 Potential
Metal binding1941Calcium By similarity
Metal binding2181Calcium By similarity
Metal binding2221Calcium By similarity

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Disulfide bond54 ↔ 71 By similarity

Natural variations

Natural variant182 – 1887SHDGPPV → CNDGPPA.

Sequences

Sequence LengthMass (Da)Tools
P28744 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 7B6219C12F365DA9

FASTA39242,843
        10         20         30         40         50         60 
MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ QILPSANETR SLTTCGTYNI IDGCWRGKAD 

        70         80         90        100        110        120 
WAENRKALAD CAQGFAKGTI GGKDGDIYTV TSELDDDVAN PKEGTLRFGA AQNRPLWIIF 

       130        140        150        160        170        180 
ARDMVIRLDR ELAINNDKTI DGRGAKVEII NAGFAIYNVK NIIIHNIIMH DIVVNPGGLI 

       190        200        210        220        230        240 
KSHDGPPVPR KGSDGDAIGI SGGSQIWIDH CSLSKAVDGL IDAKHGSTHF TVSNCLFTQH 

       250        260        270        280        290        300 
QYLLLFWDFD ERGMLCTVAF NKFTDNVDQR MPNLRHGFVQ VVNNNYERWG SYALGGSAGP 

       310        320        330        340        350        360 
TILSQGNRFL ASDIKKEVVG RYGESAMSES INWNWRSYMD VFENGAIFVP SGVDPVLTPE 

       370        380        390 
QNAGMIPAEP GEAVLRLTSS AGVLSCQPGA PC 

« Hide

References

[1]"Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
Tissue: Pollen.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M80562 mRNA. Translation: AAA32670.1.
PIRD53240.

3D structure databases

ProteinModelPortalP28744.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome24. Amb a 1.
790. Amb a 1.0401.
CAZyPL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00545; UER00824.

Family and domain databases

Gene3D2.160.20.10. 1 hit.
InterProIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSPR00807. AMBALLERGEN.
SMARTSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMSSF51126. SSF51126. 1 hit.
ProtoNetSearch...

Entry information

Entry namePLY3_AMBAR
AccessionPrimary (citable) accession number: P28744
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 16, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Allergens

Nomenclature of allergens and list of entries