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P28744

- PLY3_AMBAR

UniProt

P28744 - PLY3_AMBAR

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Protein

Pectate lyase 3

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Has pectate lyase activity.By similarity

Catalytic activityi

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

Cofactori

Binds 1 calcium ion.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941CalciumBy similarity
Metal bindingi218 – 2181CalciumBy similarity
Metal bindingi222 – 2221CalciumBy similarity
Active sitei270 – 2701Sequence Analysis

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. pectate lyase activity Source: UniProtKB-EC

GO - Biological processi

  1. pectin catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00545; UER00824.

Protein family/group databases

CAZyiPL1. Polysaccharide Lyase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Pectate lyase 3 (EC:4.2.2.2)
Alternative name(s):
Antigen Amb a I
Antigen E
Short name:
AgE
Pollen allergen Amb a 1.4
Allergen: Amb a 1.4
OrganismiAmbrosia artemisiifolia (Short ragweed)
Taxonomic identifieri4212 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei24. Amb a 1.
790. Amb a 1.0401.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 392367Pectate lyase 3PRO_0000024904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi54 ↔ 71By similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Pollen and flowers.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP28744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiIPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view]
PfamiPF00544. Pec_lyase_C. 1 hit.
[Graphical view]
PRINTSiPR00807. AMBALLERGEN.
SMARTiSM00656. Amb_all. 1 hit.
[Graphical view]
SUPFAMiSSF51126. SSF51126. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ QILPSANETR SLTTCGTYNI
60 70 80 90 100
IDGCWRGKAD WAENRKALAD CAQGFAKGTI GGKDGDIYTV TSELDDDVAN
110 120 130 140 150
PKEGTLRFGA AQNRPLWIIF ARDMVIRLDR ELAINNDKTI DGRGAKVEII
160 170 180 190 200
NAGFAIYNVK NIIIHNIIMH DIVVNPGGLI KSHDGPPVPR KGSDGDAIGI
210 220 230 240 250
SGGSQIWIDH CSLSKAVDGL IDAKHGSTHF TVSNCLFTQH QYLLLFWDFD
260 270 280 290 300
ERGMLCTVAF NKFTDNVDQR MPNLRHGFVQ VVNNNYERWG SYALGGSAGP
310 320 330 340 350
TILSQGNRFL ASDIKKEVVG RYGESAMSES INWNWRSYMD VFENGAIFVP
360 370 380 390
SGVDPVLTPE QNAGMIPAEP GEAVLRLTSS AGVLSCQPGA PC
Length:392
Mass (Da):42,843
Last modified:December 1, 1992 - v1
Checksum:i7B6219C12F365DA9
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti182 – 1887SHDGPPV → CNDGPPA.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80562 mRNA. Translation: AAA32670.1.
PIRiD53240.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M80562 mRNA. Translation: AAA32670.1 .
PIRi D53240.

3D structure databases

ProteinModelPortali P28744.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 24. Amb a 1.
790. Amb a 1.0401.
CAZyi PL1. Polysaccharide Lyase Family 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00545 ; UER00824 .

Family and domain databases

Gene3Di 2.160.20.10. 1 hit.
InterProi IPR002022. Amb_allergen_dom.
IPR018082. AmbAllergen.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
[Graphical view ]
Pfami PF00544. Pec_lyase_C. 1 hit.
[Graphical view ]
PRINTSi PR00807. AMBALLERGEN.
SMARTi SM00656. Amb_all. 1 hit.
[Graphical view ]
SUPFAMi SSF51126. SSF51126. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
    Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
    Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
    Tissue: Pollen.

Entry informationi

Entry nameiPLY3_AMBAR
AccessioniPrimary (citable) accession number: P28744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 1, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3