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P28744

- PLY3_AMBAR

UniProt

P28744 - PLY3_AMBAR

Protein

Pectate lyase 3

Gene
N/A
Organism
Ambrosia artemisiifolia (Short ragweed)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
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    Functioni

    Has pectate lyase activity.By similarity

    Catalytic activityi

    Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends.

    Cofactori

    Binds 1 calcium ion.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi194 – 1941CalciumBy similarity
    Metal bindingi218 – 2181CalciumBy similarity
    Metal bindingi222 – 2221CalciumBy similarity
    Active sitei270 – 2701Sequence Analysis

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. pectate lyase activity Source: UniProtKB-EC

    GO - Biological processi

    1. pectin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Ligandi

    Calcium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00545; UER00824.

    Protein family/group databases

    CAZyiPL1. Polysaccharide Lyase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pectate lyase 3 (EC:4.2.2.2)
    Alternative name(s):
    Antigen Amb a I
    Antigen E
    Short name:
    AgE
    Pollen allergen Amb a 1.4
    Allergen: Amb a 1.4
    OrganismiAmbrosia artemisiifolia (Short ragweed)
    Taxonomic identifieri4212 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsAsteralesAsteraceaeAsteroideaeHeliantheae allianceHeliantheaeAmbrosia

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. This is one of the major allergens of the ragweed pollen.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei24. Amb a 1.
    790. Amb a 1.0401.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Sequence AnalysisAdd
    BLAST
    Chaini26 – 392367Pectate lyase 3PRO_0000024904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi54 ↔ 71By similarity

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Pollen and flowers.

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP28744.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di2.160.20.10. 1 hit.
    InterProiIPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view]
    PfamiPF00544. Pec_lyase_C. 1 hit.
    [Graphical view]
    PRINTSiPR00807. AMBALLERGEN.
    SMARTiSM00656. Amb_all. 1 hit.
    [Graphical view]
    SUPFAMiSSF51126. SSF51126. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28744-1 [UniParc]FASTAAdd to Basket

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    MGIKHCCYIL YFTLALVTLL QPVRSAEDLQ QILPSANETR SLTTCGTYNI    50
    IDGCWRGKAD WAENRKALAD CAQGFAKGTI GGKDGDIYTV TSELDDDVAN 100
    PKEGTLRFGA AQNRPLWIIF ARDMVIRLDR ELAINNDKTI DGRGAKVEII 150
    NAGFAIYNVK NIIIHNIIMH DIVVNPGGLI KSHDGPPVPR KGSDGDAIGI 200
    SGGSQIWIDH CSLSKAVDGL IDAKHGSTHF TVSNCLFTQH QYLLLFWDFD 250
    ERGMLCTVAF NKFTDNVDQR MPNLRHGFVQ VVNNNYERWG SYALGGSAGP 300
    TILSQGNRFL ASDIKKEVVG RYGESAMSES INWNWRSYMD VFENGAIFVP 350
    SGVDPVLTPE QNAGMIPAEP GEAVLRLTSS AGVLSCQPGA PC 392
    Length:392
    Mass (Da):42,843
    Last modified:December 1, 1992 - v1
    Checksum:i7B6219C12F365DA9
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti182 – 1887SHDGPPV → CNDGPPA.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80562 mRNA. Translation: AAA32670.1.
    PIRiD53240.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M80562 mRNA. Translation: AAA32670.1 .
    PIRi D53240.

    3D structure databases

    ProteinModelPortali P28744.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 24. Amb a 1.
    790. Amb a 1.0401.
    CAZyi PL1. Polysaccharide Lyase Family 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00545 ; UER00824 .

    Family and domain databases

    Gene3Di 2.160.20.10. 1 hit.
    InterProi IPR002022. Amb_allergen_dom.
    IPR018082. AmbAllergen.
    IPR012334. Pectin_lyas_fold.
    IPR011050. Pectin_lyase_fold/virulence.
    [Graphical view ]
    Pfami PF00544. Pec_lyase_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00807. AMBALLERGEN.
    SMARTi SM00656. Amb_all. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51126. SSF51126. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Sequence polymorphism of Amb a I and Amb a II, the major allergens in Ambrosia artemisiifolia (short ragweed)."
      Griffith I.J., Pollock J., Klapper D.G., Rogers B.L., Nault A.K.
      Int. Arch. Allergy Appl. Immunol. 96:296-304(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS.
      Tissue: Pollen.

    Entry informationi

    Entry nameiPLY3_AMBAR
    AccessioniPrimary (citable) accession number: P28744
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 71 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3