Reviewed, UniProtKB/Swiss-Prot P28742 (KIP1_YEAST)

Names and origin

Protein names

Recommended name:
    Kinesin-like protein KIP1
Alternative name(s):
    Chromosome instability protein 9

Gene names

Name:	KIP1
Synonyms:	CIN9
Ordered Locus Names:	YBL063W
ORF Names:	YBL0504, YBL0521

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1111 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for assembly of the mitotic spindle. Interact with spindle microtubules to produce an outwardly directed force acting upon the poles. Following spindle assembly, CIN8 and KIP1 apparently act to oppose a force that draws separated poles back together. This force seems to be mediate by KAR3.
Subunit structure	Might be dimeric.
Subcellular location	Cytoplasm › cytoskeleton › spindle. Note: Spindle microtubules that lie between the poles.
Miscellaneous	Present with 56 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the kinesin-like protein family. BimC subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW microtubule depolymerization Inferred from mutant phenotype. Source: SGD microtubule-based movement Inferred from electronic annotation. Source: InterPro mitotic anaphase B Inferred from mutant phenotype. Source: SGD mitotic sister chromatid segregation Inferred from genetic interaction. Source: SGD plasmid partitioning Inferred from mutant phenotype. Source: SGD spindle pole body separation Ref.1 Inferred from genetic interaction. Source: SGD
Cellular component	kinesin complex Traceable author statement. Source: SGD spindle microtubule Ref.1 Inferred from direct assay. Source: SGD spindle pole body Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct microtubule motor activity Traceable author statement. Source: SGD structural constituent of cytoskeleton Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-9740,EBI-9740
TEF1	P02994	1	EBI-9740,EBI-6314

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1111	1111	Kinesin-like protein KIP1		PRO_0000125369
Regions
	Domain	50 – 423	374	Kinesin-motor
	Nucleotide binding	141 – 148	8	ATP By similarity
	Coiled coil	424 – 510	87	Potential
	Coiled coil	648 – 670	23	Potential
	Coiled coil	710 – 780	71	Potential
	Coiled coil	808 – 828	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P28742-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 212F8279766137FC	FASTA	1,111	125,795
	10 20 30 40 50 60 MARSSLPNRR TAQFEANKRR TIAHAPSPSL SNGMHTLTPP TCNNGAATSD SNIHVYVRCR 70 80 90 100 110 120 SRNKREIEEK SSVVISTLGP QGKEIILSNG SHQSYSSSKK TYQFDQVFGA ESDQETVFNA 130 140 150 160 170 180 TAKNYIKEML HGYNCTIFAY GQTGTGKTYT MSGDINILGD VQSTDNLLLG EHAGIIPRVL 190 200 210 220 230 240 VDLFKELSSL NKEYSVKISF LELYNENLKD LLSDSEDDDP AVNDPKRQIR IFDNNNNNSS 250 260 270 280 290 300 IMVKGMQEIF INSAHEGLNL LMQGSLKRKV AATKCNDLSS RSHTVFTITT NIVEQDSKDH 310 320 330 340 350 360 GQNKNFVKIG KLNLVDLAGS ENINRSGAEN KRAQEAGLIN KSLLTLGRVI NALVDHSNHI 370 380 390 400 410 420 PYRESKLTRL LQDSLGGMTK TCIIATISPA KISMEETAST LEYATRAKSI KNTPQVNQSL 430 440 450 460 470 480 SKDTCLKDYI QEIEKLRNDL KNSRNKQGIF ITQDQLDLYE SNSILIDEQN LKIHNLREQI 490 500 510 520 530 540 KKFKENYLNQ LDINNLLQSE KEKLIAIIQN FNVDFSNFYS EIQKIHHTNL ELMNEVIQQR 550 560 570 580 590 600 DFSLENSQKQ YNTNQNMQLK ISQQVLQTLN TLQGSLNNYN SKCSEVIKGV TEELTRNVNT 610 620 630 640 650 660 HKAKHDSTLK SLLNITTNLL MNQMNELVRS ISTSLEIFQS DSTSHYRKDL NEIYQSHQQF 670 680 690 700 710 720 LKNLQNDIKS CLDSIGSSIL TSINEISQNC TTNLNSMNVL IENQQSGSSK LIKEQDLEIK 730 740 750 760 770 780 KLKNDLINER RISNQFNQQL AEMKRYFQDH VSRTRSEFHD ELNKCIDNLK DKQSKLDQDI 790 800 810 820 830 840 WQKTASIFNE TDIVVNKIHS DSIASLAHNA ENTLKTVSQN NESFTNDLIS LSRGMNMDIS 850 860 870 880 890 900 SKLRSLPINE FLNKISQTIC ETCGDDNTIA SNPVLTSIKK FQNIICSDIA LTNEKIMSLI 910 920 930 940 950 960 DEIQSQIETI SNENNINLIA INENFNSLCN FILTDYDENI MQISKTQDEV LSEHCEKLQS 970 980 990 1000 1010 1020 LKILGMDIFT AHSIEKPLHE HTRPEASVIK ALPLLDYPKQ FQIYRDAENK SKDDTSNSRT 1030 1040 1050 1060 1070 1080 CIPNLSTNEN FPLSQFSPKT PVPVPDQPLP KVLIPKSINS AKSNRSKTLP NTEGTGRESQ 1090 1100 1110 NNLKRRFTTE PILKGEETEN NDILQNKKLH Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kinesin-related proteins required for assembly of the mitotic spindle." Roof D.M., Meluh P.B., Rose M.D. J. Cell Biol. 118:95-108(1992) [PubMed: 1618910] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Sequencing and functional analysis of a 32,560 bp segment on the left arm of yeast chromosome II. Identification of 26 open reading frames, including the KIP1 and SEC17 genes." Scherens B., el Bakkoury M., Vierendeels F., Dubois E., Messenguy F. Yeast 9:1355-1371(1993) [PubMed: 8154187] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete DNA sequence of yeast chromosome II." Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C. , Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K. EMBO J. 13:5795-5809(1994) [PubMed: 7813418] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Kinesin-related proteins required for structural integrity of the mitotic spindle." Saunders W.S., Hoyt M.A. Cell 70:451-458(1992) [PubMed: 1643659] [Abstract] Cited for: CHARACTERIZATION. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z11962 Genomic DNA. Translation: CAA78019.1. Z23261 Genomic DNA. Translation: CAA80785.1. Z35824 Genomic DNA. Translation: CAA84883.1.
PIR	A42640.
RefSeq	NP_009490.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:174N.
IntAct	P28742. 2 interactions.
STRING	P28742.
Genome annotation databases
Ensembl	YBL063W; YBL063W; YBL063W; Saccharomyces cerevisiae. [Genome view]
GeneID	852216.
KEGG	sce:YBL063W.
NMPDR	fig|4932.3.peg.179.
Organism-specific databases
CYGD	YBL063w.
SGD	S000000159. KIP1.
Phylogenomic databases
HOGENOM	P28742.
OMA	INERRIS
OrthoDB	EOG9TB5TK
Gene expression databases
ArrayExpress	P28742.
Genevestigator	P28742.
GermOnline	YBL063W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001752. Kinesin_motor. IPR019821. Kinesin_motor_CS. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	970727.

Entry information

Entry name

KIP1_YEAST

Accession

Primary (citable) accession number: P28742

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	November 24, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)