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Protein

Kinesin-like protein KIF3A

Gene

Kif3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 107ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • centriole-centriole cohesion Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • dentate gyrus development Source: MGI
  • determination of left/right symmetry Source: MGI
  • dorsal/ventral neural tube patterning Source: BHF-UCL
  • dorsal/ventral pattern formation Source: MGI
  • epidermal stem cell homeostasis Source: MGI
  • epidermis development Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • inner ear receptor stereocilium organization Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • membrane organization Source: Reactome
  • microtubule anchoring at centrosome Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • motile cilium assembly Source: MGI
  • neural precursor cell proliferation Source: MGI
  • neural tube development Source: MGI
  • non-motile cilium assembly Source: MGI
  • positive regulation of axo-dendritic protein transport Source: Ensembl
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of establishment or maintenance of cell polarity regulating cell shape Source: Ensembl
  • positive regulation of receptor-mediated endocytosis Source: Ensembl
  • smoothened signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cilium biogenesis/degradation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-5610787. Hedgehog 'off' state.
R-MMU-5620924. Intraflagellar transport.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF3A
Alternative name(s):
Microtubule plus end-directed kinesin motor 3A
Gene namesi
Name:Kif3a
Synonyms:Kif3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107689. Kif3a.

Subcellular locationi

GO - Cellular componenti

  • axon cytoplasm Source: Ensembl
  • axoneme Source: Ensembl
  • centriole Source: UniProtKB
  • centrosome Source: MGI
  • cilium Source: UniProtKB
  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • kinesin II complex Source: MGI
  • microtubule Source: UniProtKB-KW
  • microtubule cytoskeleton Source: MGI
  • motile cilium Source: MGI
  • neuronal cell body Source: Ensembl
  • neuron projection Source: MGI
  • photoreceptor connecting cilium Source: UniProtKB
  • ribbon synapse Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001253941 – 701Kinesin-like protein KIF3AAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei689PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP28741.
MaxQBiP28741.
PaxDbiP28741.
PRIDEiP28741.

PTM databases

iPTMnetiP28741.
PhosphoSitePlusiP28741.

Expressioni

Tissue specificityi

Expressed almost exclusively in adult brain tissue (mainly in the cerebellar granular layer) within a single type of neuronal cell.

Gene expression databases

BgeeiENSMUSG00000018395.
CleanExiMM_KIF3A.
ExpressionAtlasiP28741. baseline and differential.
GenevisibleiP28741. MM.

Interactioni

Subunit structurei

Heterodimer of KIF3A and KIF3B (PubMed:7559760). Interacts with PIFO (PubMed:20643351). Interacts with CLN3 (By similarity). Interacts with DCTN1 (PubMed:23386061).By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cep290Q6A0782EBI-6169413,EBI-1811999
Dctn1O087884EBI-6169413,EBI-776180
Kif3bQ617714EBI-6169413,EBI-6395332
Trim60Q8VI405EBI-6169413,EBI-6395249

GO - Molecular functioni

  • microtubule binding Source: MGI
  • Rab GTPase binding Source: MGI
  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi200941. 3 interactors.
DIPiDIP-46318N.
IntActiP28741. 11 interactors.
STRINGi10090.ENSMUSP00000112782.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JVRX-ray2.10A/B/C/D/E/F/G/H352-359[»]
5JX1X-ray1.67A352-376[»]
ProteinModelPortaliP28741.
SMRiP28741.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 345Kinesin motorPROSITE-ProRule annotationAdd BLAST332

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 701Interaction with DCTN11 PublicationAdd BLAST337
Regioni599 – 701GlobularAdd BLAST103

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili355 – 592Sequence analysisAdd BLAST238

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi442 – 445Poly-Glu4
Compositional biasi509 – 512Poly-Arg4

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.PROSITE-ProRule annotation
Contains 1 kinesin motor domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000116164.
HOVERGENiHBG052255.
InParanoidiP28741.
KOiK10394.
TreeFamiTF105223.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYRQAVSVD EMRGTITVHK
60 70 80 90 100
TDSSNEPPKT FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG
110 120 130 140 150
QTGTGKTFTM EGVRAVPGLR GVIPNSFAHI FGHIAKAEGD TRFLVRVSYL
160 170 180 190 200
EIYNEEVRDL LGKDQTQRLE VKERPDVGVY IKDLSAYVVN NADDMDRIMT
210 220 230 240 250
LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGVDGNMH VRMGKLHLVD
260 270 280 290 300
LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS
310 320 330 340 350
KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR
360 370 380 390 400
INEDPKDALL RQFQKEIEEL KKKLEEGEEV SGSDISGSEE DDEEGELGED
410 420 430 440 450
GEKKKKRRDQ AGKKKVSPDK MVEMQAKIDE ERKALETKLD MEEEERNKAR
460 470 480 490 500
AELERREKDL LKAQQEHQSL LEKLSALEKK VIVGGVDLLA KAEEQEKLLE
510 520 530 540 550
ESNMELEERR RRAEQLRKEL EEKEQERLDI EEKYTSLQEE AQGKTKKLKK
560 570 580 590 600
VWTMLMAAKS EMADLQQEHQ REIEGLLENI RQLSRELRLQ MLIIDNFIPQ
610 620 630 640 650
DYQEMIENYV HWNEDIGEWQ LKCVAYTGNN MRKQTPVPDK KERDPFEVDL
660 670 680 690 700
SHVYLAYTEE SLRQSLMKLE RPRTSKGKAR PKMGRRKRSA KPETVIDSLL

Q
Length:701
Mass (Da):80,170
Last modified:July 27, 2011 - v2
Checksum:i15EB43A6F6CC08A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti404K → R in BAA02166 (PubMed:1447303).Curated1
Sequence conflicti683M → T in BAA02166 (PubMed:1447303).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12645 mRNA. Translation: BAA02166.1.
AL596095 Genomic DNA. Translation: CAP19148.1.
BC052707 mRNA. Translation: AAH52707.1.
CCDSiCCDS48795.1.
PIRiB44259.
RefSeqiNP_001277734.1. NM_001290805.1.
NP_001277735.1. NM_001290806.1.
NP_032469.2. NM_008443.4.
UniGeneiMm.88694.

Genome annotation databases

EnsembliENSMUST00000120613; ENSMUSP00000112782; ENSMUSG00000018395.
GeneIDi16568.
KEGGimmu:16568.
UCSCiuc007iwl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12645 mRNA. Translation: BAA02166.1.
AL596095 Genomic DNA. Translation: CAP19148.1.
BC052707 mRNA. Translation: AAH52707.1.
CCDSiCCDS48795.1.
PIRiB44259.
RefSeqiNP_001277734.1. NM_001290805.1.
NP_001277735.1. NM_001290806.1.
NP_032469.2. NM_008443.4.
UniGeneiMm.88694.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JVRX-ray2.10A/B/C/D/E/F/G/H352-359[»]
5JX1X-ray1.67A352-376[»]
ProteinModelPortaliP28741.
SMRiP28741.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200941. 3 interactors.
DIPiDIP-46318N.
IntActiP28741. 11 interactors.
STRINGi10090.ENSMUSP00000112782.

PTM databases

iPTMnetiP28741.
PhosphoSitePlusiP28741.

Proteomic databases

EPDiP28741.
MaxQBiP28741.
PaxDbiP28741.
PRIDEiP28741.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000120613; ENSMUSP00000112782; ENSMUSG00000018395.
GeneIDi16568.
KEGGimmu:16568.
UCSCiuc007iwl.2. mouse.

Organism-specific databases

CTDi11127.
MGIiMGI:107689. Kif3a.

Phylogenomic databases

eggNOGiKOG0240. Eukaryota.
COG5059. LUCA.
GeneTreeiENSGT00840000129680.
HOGENOMiHOG000116164.
HOVERGENiHBG052255.
InParanoidiP28741.
KOiK10394.
TreeFamiTF105223.

Enzyme and pathway databases

ReactomeiR-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-5610787. Hedgehog 'off' state.
R-MMU-5620924. Intraflagellar transport.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5635838. Activation of SMO.
R-MMU-6811434. COPI-dependent Golgi-to-ER retrograde traffic.
R-MMU-983189. Kinesins.

Miscellaneous databases

ChiTaRSiKif3a. mouse.
PROiP28741.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018395.
CleanExiMM_KIF3A.
ExpressionAtlasiP28741. baseline and differential.
GenevisibleiP28741. MM.

Family and domain databases

Gene3Di3.40.850.10. 1 hit.
InterProiIPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR24115. PTHR24115. 1 hit.
PfamiPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSiPR00380. KINESINHEAVY.
SMARTiSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKIF3A_MOUSE
AccessioniPrimary (citable) accession number: P28741
Secondary accession number(s): Q7TSZ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.