P28741 (KIF3A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kinesin-like protein KIF3A

Alternative name(s):
Microtubule plus end-directed kinesin motor 3A

Gene names

Name:	Kif3a
Synonyms:	Kif3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation. Ref.7
Subunit structure	Heterodimer of KIF3A and KIF3B. Interacts with PIFO. Ref.6
Subcellular location	Cytoplasm › cytoskeleton Probable. Cell projection › cilium Ref.7.
Tissue specificity	Expressed almost exclusively in adult brain tissue (mainly in the cerebellar granular layer) within a single type of neuronal cell.
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Biological process	Cilium biogenesis/degradation
Cellular component	Cell projection Cilium Cytoplasm Cytoskeleton Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	anterior/posterior pattern specification Inferred from mutant phenotype. Source: MGI cilium assembly Inferred from mutant phenotype Ref.7. Source: UniProtKB determination of left/right symmetry Inferred from mutant phenotype. Source: MGI dorsal/ventral neural tube patterning Inferred from mutant phenotype. Source: MGI heart development Inferred from mutant phenotype. Source: MGI in utero embryonic development Inferred from genetic interaction. Source: MGI kidney development Inferred from mutant phenotype. Source: MGI microtubule-based movement Inferred from electronic annotation. Source: InterPro smoothened signaling pathway Inferred from mutant phenotype. Source: MGI
Cellular component	cytosol Traceable author statement. Source: Reactome microtubule Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor connecting cilium Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Kinesin-like protein KIF3A		PRO_0000125394
Regions
	Domain	1 – 350	350	Kinesin-motor
	Nucleotide binding	100 – 107	8	ATP By similarity
	Region	599 – 701	103	Globular
	Coiled coil	355 – 592	238	Potential
	Compositional bias	442 – 445	4	Poly-Glu
	Compositional bias	509 – 512	4	Poly-Arg
Amino acid modifications
	Modified residue	635	1	Phosphothreonine Ref.5
	Modified residue	689	1	Phosphoserine Ref.4
Experimental info
	Sequence conflict	404	1	K → R in BAA02166. Ref.1
	Sequence conflict	683	1	M → T in BAA02166. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28741 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 15EB43A6F6CC08A3	FASTA	701	80,170
	10 20 30 40 50 60 MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYRQAVSVD EMRGTITVHK TDSSNEPPKT 70 80 90 100 110 120 FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG QTGTGKTFTM EGVRAVPGLR 130 140 150 160 170 180 GVIPNSFAHI FGHIAKAEGD TRFLVRVSYL EIYNEEVRDL LGKDQTQRLE VKERPDVGVY 190 200 210 220 230 240 IKDLSAYVVN NADDMDRIMT LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGVDGNMH 250 260 270 280 290 300 VRMGKLHLVD LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS 310 320 330 340 350 360 KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR INEDPKDALL 370 380 390 400 410 420 RQFQKEIEEL KKKLEEGEEV SGSDISGSEE DDEEGELGED GEKKKKRRDQ AGKKKVSPDK 430 440 450 460 470 480 MVEMQAKIDE ERKALETKLD MEEEERNKAR AELERREKDL LKAQQEHQSL LEKLSALEKK 490 500 510 520 530 540 VIVGGVDLLA KAEEQEKLLE ESNMELEERR RRAEQLRKEL EEKEQERLDI EEKYTSLQEE 550 560 570 580 590 600 AQGKTKKLKK VWTMLMAAKS EMADLQQEHQ REIEGLLENI RQLSRELRLQ MLIIDNFIPQ 610 620 630 640 650 660 DYQEMIENYV HWNEDIGEWQ LKCVAYTGNN MRKQTPVPDK KERDPFEVDL SHVYLAYTEE 670 680 690 700 SLRQSLMKLE RPRTSKGKAR PKMGRRKRSA KPETVIDSLL Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kinesin family in murine central nervous system." Aizawa H., Sekine Y., Takemura R., Zhang Z., Nangaku M., Hirokawa N. J. Cell Biol. 119:1287-1296(1992) [PubMed: 1447303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY. Tissue: Embryonic brain.
[5]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 19367708] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, MASS SPECTROMETRY. Tissue: Melanoma.
[6]	"Pitchfork regulates primary cilia disassembly and left-right asymmetry." Kinzel D., Boldt K., Davis E.E., Burtscher I., Trumbach D., Diplas B., Attie-Bitach T., Wurst W., Katsanis N., Ueffing M., Lickert H. Dev. Cell 19:66-77(2010) [PubMed: 20643351] [Abstract] Cited for: INTERACTION WITH PIFO.
[7]	"Polycystin-2 and phosphodiesterase 4C are components of a ciliary A-kinase anchoring protein complex that is disrupted in cystic kidney diseases." Choi Y.H., Suzuki A., Hajarnis S., Ma Z., Chapin H.C., Caplan M.J., Pontoglio M., Somlo S., Igarashi P. Proc. Natl. Acad. Sci. U.S.A. 108:10679-10684(2011) [PubMed: 21670265] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12645 mRNA. Translation: BAA02166.1. AL596095 Genomic DNA. Translation: CAP19148.1. BC052707 mRNA. Translation: AAH52707.1.
IPI	IPI00881566.
PIR	B44259.
RefSeq	NP_032469.2. NM_008443.3.
UniGene	Mm.88694.
3D structure databases
HSSP	HSSP built from PDB template 1F9T based on UniProtKB P17119.
ProteinModelPortal	P28741.
SMR	P28741. Positions 13-363.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46318N.
STRING	P28741.
PTM databases
PhosphoSite	P28741.
Proteomic databases
PRIDE	P28741.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000108958; ENSMUSP00000104586; ENSMUSG00000018395. ENSMUST00000120613; ENSMUSP00000112782; ENSMUSG00000018395.
GeneID	16568.
KEGG	mmu:16568.
Organism-specific databases
CTD	11127.
MGI	MGI:107689. Kif3a.
Phylogenomic databases
eggNOG	roNOG04329.
HOVERGEN	HBG052255.
Gene expression databases
ArrayExpress	P28741.
Bgee	P28741.
CleanEx	MM_KIF3A.
Genevestigator	P28741.
GermOnline	ENSMUSG00000018395. Mus musculus.
Family and domain databases
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
KO	K10394.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	290073.
SOURCE	Search...

Entry information

Entry name

KIF3A_MOUSE

Accession

Primary (citable) accession number: P28741
Secondary accession number(s): Q7TSZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	July 27, 2011
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program