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Protein

Kinesin-like protein KIF3A

Gene

Kif3a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro. Plays a role in primary cilia formation (PubMed:21670265). Plays a role in centriole cohesion and subdistal appendage organization and function. Regulates the formation of the subdistal appendage via recruitement of DCTN1 to the centriole. Also required for ciliary basal feet formation and microtubule anchoring to mother centriole (PubMed:23386061).2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi100 – 107ATPPROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • centriole-centriole cohesion Source: UniProtKB
  • cilium assembly Source: UniProtKB
  • dentate gyrus development Source: MGI
  • determination of left/right symmetry Source: MGI
  • dorsal/ventral neural tube patterning Source: BHF-UCL
  • dorsal/ventral pattern formation Source: MGI
  • epidermal stem cell homeostasis Source: MGI
  • epidermis development Source: MGI
  • heart development Source: MGI
  • heart looping Source: MGI
  • inner ear receptor cell stereocilium organization Source: MGI
  • in utero embryonic development Source: MGI
  • kidney development Source: MGI
  • microtubule anchoring at centrosome Source: UniProtKB
  • microtubule-based movement Source: GO_Central
  • motile cilium assembly Source: MGI
  • neural precursor cell proliferation Source: MGI
  • neural tube development Source: MGI
  • non-motile cilium assembly Source: MGI
  • positive regulation of axo-dendritic protein transport Source: Ensembl
  • positive regulation of epithelial cell proliferation Source: MGI
  • positive regulation of establishment or maintenance of cell polarity regulating cell shape Source: Ensembl
  • positive regulation of receptor-mediated endocytosis Source: Ensembl
  • smoothened signaling pathway Source: MGI

Keywordsi

Molecular functionMotor protein
Biological processCilium biogenesis/degradation
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295 MHC class II antigen presentation
R-MMU-5610787 Hedgehog 'off' state
R-MMU-5620924 Intraflagellar transport
R-MMU-5632684 Hedgehog 'on' state
R-MMU-5635838 Activation of SMO
R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic
R-MMU-983189 Kinesins

Names & Taxonomyi

Protein namesi
Recommended name:
Kinesin-like protein KIF3A
Alternative name(s):
Microtubule plus end-directed kinesin motor 3A
Gene namesi
Name:Kif3a
Synonyms:Kif3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:107689 Kif3a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001253941 – 701Kinesin-like protein KIF3AAdd BLAST701

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei689PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP28741
MaxQBiP28741
PaxDbiP28741
PRIDEiP28741

PTM databases

iPTMnetiP28741
PhosphoSitePlusiP28741

Expressioni

Tissue specificityi

Expressed almost exclusively in adult brain tissue (mainly in the cerebellar granular layer) within a single type of neuronal cell.

Gene expression databases

BgeeiENSMUSG00000018395
CleanExiMM_KIF3A
ExpressionAtlasiP28741 baseline and differential
GenevisibleiP28741 MM

Interactioni

Subunit structurei

Heterodimer of KIF3A and KIF3B (PubMed:7559760). Interacts with PIFO (PubMed:20643351). Interacts with CLN3 (By similarity). Interacts with DCTN1 (PubMed:23386061).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • kinesin binding Source: Ensembl
  • microtubule binding Source: MGI
  • Rab GTPase binding Source: MGI
  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi200941, 4 interactors
CORUMiP28741
DIPiDIP-46318N
IntActiP28741, 14 interactors
MINTiP28741
STRINGi10090.ENSMUSP00000112782

Structurei

Secondary structure

1701
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi355 – 376Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5JVRX-ray2.10A/B/C/D/E/F/G/H352-359[»]
5JX1X-ray1.67A352-376[»]
ProteinModelPortaliP28741
SMRiP28741
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 345Kinesin motorPROSITE-ProRule annotationAdd BLAST332

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni365 – 701Interaction with DCTN11 PublicationAdd BLAST337
Regioni599 – 701GlobularAdd BLAST103

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili355 – 592Sequence analysisAdd BLAST238

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi442 – 445Poly-Glu4
Compositional biasi509 – 512Poly-Arg4

Sequence similaritiesi

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin II subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0240 Eukaryota
COG5059 LUCA
GeneTreeiENSGT00910000143992
HOGENOMiHOG000116164
HOVERGENiHBG052255
InParanoidiP28741
KOiK10394
TreeFamiTF105223

Family and domain databases

Gene3Di3.40.850.10, 1 hit
InterProiView protein in InterPro
IPR027640 Kinesin-like_fam
IPR019821 Kinesin_motor_CS
IPR001752 Kinesin_motor_dom
IPR036961 Kinesin_motor_dom_sf
IPR027417 P-loop_NTPase
PANTHERiPTHR24115 PTHR24115, 1 hit
PfamiView protein in Pfam
PF00225 Kinesin, 1 hit
PRINTSiPR00380 KINESINHEAVY
SMARTiView protein in SMART
SM00129 KISc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00411 KINESIN_MOTOR_1, 1 hit
PS50067 KINESIN_MOTOR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28741-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYRQAVSVD EMRGTITVHK
60 70 80 90 100
TDSSNEPPKT FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG
110 120 130 140 150
QTGTGKTFTM EGVRAVPGLR GVIPNSFAHI FGHIAKAEGD TRFLVRVSYL
160 170 180 190 200
EIYNEEVRDL LGKDQTQRLE VKERPDVGVY IKDLSAYVVN NADDMDRIMT
210 220 230 240 250
LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGVDGNMH VRMGKLHLVD
260 270 280 290 300
LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS
310 320 330 340 350
KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR
360 370 380 390 400
INEDPKDALL RQFQKEIEEL KKKLEEGEEV SGSDISGSEE DDEEGELGED
410 420 430 440 450
GEKKKKRRDQ AGKKKVSPDK MVEMQAKIDE ERKALETKLD MEEEERNKAR
460 470 480 490 500
AELERREKDL LKAQQEHQSL LEKLSALEKK VIVGGVDLLA KAEEQEKLLE
510 520 530 540 550
ESNMELEERR RRAEQLRKEL EEKEQERLDI EEKYTSLQEE AQGKTKKLKK
560 570 580 590 600
VWTMLMAAKS EMADLQQEHQ REIEGLLENI RQLSRELRLQ MLIIDNFIPQ
610 620 630 640 650
DYQEMIENYV HWNEDIGEWQ LKCVAYTGNN MRKQTPVPDK KERDPFEVDL
660 670 680 690 700
SHVYLAYTEE SLRQSLMKLE RPRTSKGKAR PKMGRRKRSA KPETVIDSLL

Q
Length:701
Mass (Da):80,170
Last modified:July 27, 2011 - v2
Checksum:i15EB43A6F6CC08A3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti404K → R in BAA02166 (PubMed:1447303).Curated1
Sequence conflicti683M → T in BAA02166 (PubMed:1447303).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D12645 mRNA Translation: BAA02166.1
AL596095 Genomic DNA No translation available.
BC052707 mRNA Translation: AAH52707.1
CCDSiCCDS48795.1
PIRiB44259
RefSeqiNP_001277734.1, NM_001290805.1
NP_001277735.1, NM_001290806.1
NP_032469.2, NM_008443.4
UniGeneiMm.88694

Genome annotation databases

EnsembliENSMUST00000120613; ENSMUSP00000112782; ENSMUSG00000018395
GeneIDi16568
KEGGimmu:16568
UCSCiuc007iwl.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKIF3A_MOUSE
AccessioniPrimary (citable) accession number: P28741
Secondary accession number(s): Q7TSZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 157 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health