Reviewed, UniProtKB/Swiss-Prot P28741 (KIF3A_MOUSE)

Names and origin

Protein names

Recommended name:
    Kinesin-like protein KIF3A
Alternative name(s):
    Microtubule plus end-directed kinesin motor 3A

Gene names

Name:	Kif3a
Synonyms:	Kif3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Microtubule-based anterograde translocator for membranous organelles. Plus end-directed microtubule sliding activity in vitro.
Subunit structure	Heterodimer of KIF3A and KIF3B.
Tissue specificity	Expressed almost exclusively in adult brain tissue (mainly in the cerebellar granular layer) within a single type of neuronal cell.
Sequence similarities	Belongs to the kinesin-like protein family. Kinesin II subfamily. Contains 1 kinesin-motor domain.

Ontologies

Keywords
Cellular component	Microtubule
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Motor protein
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	anterior/posterior pattern formation Inferred from mutant phenotype. Source: MGI cilium assembly Inferred from mutant phenotype. Source: MGI determination of left/right symmetry Inferred from mutant phenotype. Source: MGI dorsal/ventral neural tube patterning Inferred from mutant phenotype. Source: MGI heart development Inferred from mutant phenotype. Source: MGI in utero embryonic development Inferred from genetic interaction. Source: MGI kidney development Inferred from mutant phenotype. Source: MGI microtubule-based movement Inferred from electronic annotation. Source: InterPro smoothened signaling pathway Inferred from mutant phenotype. Source: MGI
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Kinesin-like protein KIF3A		PRO_0000125394
Regions
	Domain	1 – 350	350	Kinesin-motor
	Nucleotide binding	100 – 107	8	ATP By similarity
	Region	599 – 701	103	Globular
	Coiled coil	355 – 592	238	Potential
	Compositional bias	442 – 445	4	Poly-Glu
	Compositional bias	509 – 512	4	Poly-Arg
Amino acid modifications
	Modified residue	635	1	Phosphothreonine Ref.3
	Modified residue	689	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28741-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 2405872DF2D85A29	FASTA	701	80,168
	10 20 30 40 50 60 MPINKSEKPE SCDNVKVVVR CRPLNEREKS MCYRQAVSVD EMRGTITVHK TDSSNEPPKT 70 80 90 100 110 120 FTFDTVFGPE SKQLDVYNLT ARPIIDSVLE GYNGTIFAYG QTGTGKTFTM EGVRAVPGLR 130 140 150 160 170 180 GVIPNSFAHI FGHIAKAEGD TRFLVRVSYL EIYNEEVRDL LGKDQTQRLE VKERPDVGVY 190 200 210 220 230 240 IKDLSAYVVN NADDMDRIMT LGHKNRSVGA TNMNEHSSRS HAIFTITIEC SEKGVDGNMH 250 260 270 280 290 300 VRMGKLHLVD LAGSERQAKT GATGQRLKEA TKINLSLSTL GNVISALVDG KSTHVPYRNS 310 320 330 340 350 360 KLTRLLQDSL GGNSKTMMCA NIGPADYNYD ETISTLRYAN RAKNIKNKAR INEDPKDALL 370 380 390 400 410 420 RQFQKEIEEL KKKLEEGEEV SGSDISGSEE DDEEGELGED GEKRKKRRDQ AGKKKVSPDK 430 440 450 460 470 480 MVEMQAKIDE ERKALETKLD MEEEERNKAR AELERREKDL LKAQQEHQSL LEKLSALEKK 490 500 510 520 530 540 VIVGGVDLLA KAEEQEKLLE ESNMELEERR RRAEQLRKEL EEKEQERLDI EEKYTSLQEE 550 560 570 580 590 600 AQGKTKKLKK VWTMLMAAKS EMADLQQEHQ REIEGLLENI RQLSRELRLQ MLIIDNFIPQ 610 620 630 640 650 660 DYQEMIENYV HWNEDIGEWQ LKCVAYTGNN MRKQTPVPDK KERDPFEVDL SHVYLAYTEE 670 680 690 700 SLRQSLMKLE RPRTSKGKAR PKTGRRKRSA KPETVIDSLL Q

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Kinesin family in murine central nervous system." Aizawa H., Sekine Y., Takemura R., Zhang Z., Nangaku M., Hirokawa N. J. Cell Biol. 119:1287-1296(1992) [PubMed: 1447303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-689, MASS SPECTROMETRY. Tissue: Brain.
[3]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-635, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D12645 mRNA. Translation: BAA02166.1.
IPI	IPI00312076.
PIR	B44259.
UniGene	Mm.88694
3D structure databases
SMR	P28741. Positions 13-363.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46318N.
STRING	P28741.
PTM databases
PhosphoSite	P28741.
Proteomic databases
PRIDE	P28741.
Genome annotation databases
Ensembl	ENSMUST00000108958; ENSMUSP00000104586; ENSMUSG00000018395; Mus musculus. [Genome view] ENSMUST00000120613; ENSMUSP00000112782; ENSMUSG00000018395; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:107689. Kif3a.
Phylogenomic databases
eggNOG	roNOG04329.
HOVERGEN	P28741.
Gene expression databases
ArrayExpress	P28741.
Bgee	P28741.
CleanEx	MM_KIF3A.
Genevestigator	P28741.
GermOnline	ENSMUSG00000018395. Mus musculus.
Family and domain databases
InterPro	IPR019821. Kinesin_motor_CS. IPR001752. Kinesin_motor_dom. [Graphical view]
Gene3D	G3DSA:3.40.850.10. kinesin_motor. 1 hit.
Pfam	PF00225. Kinesin. 1 hit. [Graphical view]
PRINTS	PR00380. KINESINHEAVY.
SMART	SM00129. KISc. 1 hit. [Graphical view]
PROSITE	PS00411. KINESIN_MOTOR_DOMAIN1. 1 hit. PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

KIF3A_MOUSE

Accession

Primary (citable) accession number: P28741

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	February 9, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)