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P28740 (KIF2A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF2A
Alternative name(s):
Kinesin-2
Gene names
Name:Kif2a
Synonyms:Kif2, Kns2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length705 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plus end-directed microtubule-dependent motor required for normal brain development. May regulate microtubule dynamics during axonal growth. Required for normal progression through mitosis. Required for normal congress of chromosomes at the metaphase plate. Required for normal spindle dynamics during mitosis. Promotes spindle turnover. Implicated in formation of bipolar mitotic spindles. Has microtubule depolymerization activity By similarity. Ref.4

Subunit structure

Interacts with AURKA, PSRC1 and PLK1 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › centrosome. Cytoplasm › cytoskeleton › spindle pole. Cytoplasm › cytoskeleton › spindle By similarity. Lysosome. Note: Localized to the spindle microtubules and spindle poles from prophase to metaphase. Efficient targeting to spindle microtubules and spindle poles requires the kinase activity of PLK1. Recruited to mitotic spindles by interaction with PSRC1 By similarity. Associated with lysosomes in NIH3T3 cells. Ref.2

Tissue specificity

Highest level in lung. High level in ovary, moderate levels in heart, kidney, placenta, skeletal muscle and spleen (at protein level). Pancreas and spleen express a shorter at protein level). Isoform 1 expressed in neuronal cells. Isoform 2 expressed in astrocytes and fibroblasts. Ref.2

Developmental stage

Isoform 1 expressed at low level in E13 embryonic hippocampus, higher level by stage 15 persisting into juvenile and adult stages. Isoform 2 expressed in E13 and E15 embryonic hippocampus declining to very low levels in juvenile and adult neurons. High level of isoform 1 and very low level of isoform 2 in stage 2 and 5 hippocampal neurons in culture. Ref.2

Disruption phenotype

Mice show overextension of collateral branches of developing axons and defects in neuronal migration in the brain. They die within 24 hours of birth. NIH3T3 cells overexpressing isoform 2 show enlarged lysosomal structures and a defect in their perinuclear localization. Ref.4

Sequence similarities

Belongs to the kinesin-like protein family. MCAK/KIF2 subfamily.

Contains 1 kinesin-motor domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Differentiation
Mitosis
Neurogenesis
   Cellular componentCytoplasm
Cytoskeleton
Lysosome
Microtubule
   Coding sequence diversityAlternative splicing
   DomainCoiled coil
   LigandATP-binding
Nucleotide-binding
   Molecular functionDevelopmental protein
Motor protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell differentiation

Inferred from electronic annotation. Source: UniProtKB-KW

cell division

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule-based movement

Inferred from electronic annotation. Source: InterPro

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

mitotic spindle organization

Inferred from sequence or structural similarity. Source: UniProtKB

nervous system development

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcentrosome

Inferred from electronic annotation. Source: Compara

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

microtubule associated complex

Inferred from electronic annotation. Source: InterPro

spindle microtubule

Inferred from electronic annotation. Source: Compara

spindle pole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

microtubule motor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 3 (identifier: P28740-3)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1 (identifier: P28740-1)

Also known as: Kif2;

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.
     551-551: E → EFGISPSDIPFSQGGGSRPDLSPSYDYDDFSPSITRVKE
Isoform 2 (identifier: P28740-2)

Also known as: Kif2-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.
     133-152: GSVSDISPVQAAKKEFGPPS → A
Note: Contains a phosphoserine at position 554.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 705705Kinesin-like protein KIF2A
PRO_0000125415

Regions

Domain217 – 566350Kinesin-motor
Nucleotide binding312 – 3198ATP By similarity
Region1 – 216216Globular Potential
Coiled coil659 – 69840 Potential

Amino acid modifications

Modified residue751Phosphoserine By similarity
Modified residue5711Phosphoserine Ref.5

Natural variations

Alternative sequence1 – 2727Missing in isoform 1 and isoform 2.
VSP_028377
Alternative sequence133 – 15220GSVSD…FGPPS → A in isoform 2.
VSP_028378
Alternative sequence5511E → EFGISPSDIPFSQGGGSRPD LSPSYDYDDFSPSITRVKE in isoform 1.
VSP_028379

Experimental info

Sequence conflict991A → ARA in CAA75815. Ref.2
Sequence conflict117 – 1182Missing in CAA75815. Ref.2
Sequence conflict4861A → R in BAA02165. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 3 [UniParc].

Last modified October 2, 2007. Version 2.
Checksum: ACEBB35CC0F4A68E

FASTA70579,756
        10         20         30         40         50         60 
MATANFGKIQ IGIYVEIKRS DGRIHQAMVT SLNEDNESVT VEWIENGDTK GKEIDLESIF 

        70         80         90        100        110        120 
SLNPDLVPDE DIEPSPELPP PSSSSKVNKI VKNRRTVAAV KNDPPPRDNR VVGSARARPS 

       130        140        150        160        170        180 
QLPEQSSSAQ QNGSVSDISP VQAAKKEFGP PSRRKSNCVK EVEKLQEKRE KRRLQQQELR 

       190        200        210        220        230        240 
EKRAQDVDAT NPNYEIMCMI RDFRGSLDYR PLTTADPIDE HRICVCVRKR PLNKKETQMK 

       250        260        270        280        290        300 
DLDVITIPSK DVVMVHEPKQ KVDLTRYLEN QTFRFDYAFD DSAPNEMVYR FTARPLVETI 

       310        320        330        340        350        360 
FERGMATCFA YGQTGSGKTH TMGGDFSGKN QDCSKGIYAL AARDVFLMLK KPNYKKLELQ 

       370        380        390        400        410        420 
VYATFFEIYS GKVFDLLNRK TKLRVLEDGK QQVQVVGLQE REVKCVEDVL KLIDIGNSCR 

       430        440        450        460        470        480 
TSGQTSANAH SSRSHAVFQI ILRRKGKLHG KFSLIDLAGN ERGADTSSAD RQTRLEGAEI 

       490        500        510        520        530        540 
NKSLLALKEC IRALGRNKPH TPFRASKLTQ VLRDSFIGEN SRTCMIATIS PGMASCENTL 

       550        560        570        580        590        600 
NTLRYANRVK ELTVNPAAAG DVHPIMHHPP SQIDDLETQW GVGSSPQRDD LKLLCEQNEE 

       610        620        630        640        650        660 
EVSPQLFTFH EAVSQMVEME EQVVEDHRAV FQESIRWIED EKALLEMTEE VDYDVDSYAT 

       670        680        690        700 
QLEAILEQKI DILTELRDKV KSFRAALQEE EQASKQINPK RPRAL

« Hide

Isoform 1 (Kif2) [UniParc].

Checksum: 6655DA8F001DC51B
Show »

FASTA71680,861
Isoform 2 (Kif2-beta) [UniParc].

Checksum: 32482C9FE134CF96
Show »

FASTA65974,845

References

« Hide 'large scale' references
[1]"Kinesin family in murine central nervous system."
Aizawa H., Sekine Y., Takemura R., Zhang Z., Nangaku M., Hirokawa N.
J. Cell Biol. 119:1287-1296(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[2]"KIF2beta, a new kinesin superfamily protein in non-neuronal cells, is associated with lysosomes and may be implicated in their centrifugal translocation."
Santama N., Krijnse-Locker J., Griffiths G., Noda Y., Hirokawa N., Dotti C.G.
EMBO J. 17:5855-5867(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Strain: C57BL/6 X SJL.
Tissue: Hippocampus.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
Strain: FVB/N-3.
Tissue: Mammary gland.
[4]"Kinesin superfamily protein 2A (KIF2A) functions in suppression of collateral branch extension."
Homma N., Takei Y., Tanaka Y., Nakata T., Terada S., Kikkawa M., Noda Y., Hirokawa N.
Cell 114:229-239(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"Phosphoproteomic analysis of the developing mouse brain."
Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P.
Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-571, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-554 (ISOFORM 2), MASS SPECTROMETRY.
Tissue: Embryonic brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D12644 mRNA. Translation: BAA02165.1.
Y15894 mRNA. Translation: CAA75815.1.
BC006803 mRNA. Translation: AAH06803.2.
IPIIPI00127135.
IPI00867847.
IPI00988408.
PIRA44259.
RefSeqNP_001139251.1. NM_001145779.1.
UniGeneMm.355686.

3D structure databases

ProteinModelPortalP28740.
SMRP28740. Positions 161-549.
ModBaseSearch...

Protein-protein interaction databases

IntActP28740. 1 interaction.

PTM databases

PhosphoSiteP28740.

Proteomic databases

PaxDbP28740.
PRIDEP28740.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022204; ENSMUSP00000022204; ENSMUSG00000021693.
ENSMUST00000117423; ENSMUSP00000113921; ENSMUSG00000021693.
ENSMUST00000122233; ENSMUSP00000112715; ENSMUSG00000021693.
GeneID16563.
KEGGmmu:16563.
UCSCuc007rub.2. mouse.
uc007ruc.2. mouse.
uc007rud.2. mouse.

Organism-specific databases

CTD3796.
MGIMGI:108390. Kif2a.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00680000099804.
HOGENOMHOG000231329.
HOVERGENHBG003875.
KOK10393.
OrthoDBEOG44QT0D.

Gene expression databases

ArrayExpressP28740.
BgeeP28740.
CleanExMM_KIF2A.
GenevestigatorP28740.
GermOnlineENSMUSG00000021693. Mus musculus.

Family and domain databases

Gene3D3.40.850.10. 1 hit.
InterProIPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
[Graphical view]
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
PROSITEPS00411. KINESIN_MOTOR_DOMAIN1. 1 hit.
PS50067. KINESIN_MOTOR_DOMAIN2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio290061.
SOURCESearch...

Entry information

Entry nameKIF2A_MOUSE
AccessionPrimary (citable) accession number: P28740
Secondary accession number(s): O54744, Q91W03
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 2, 2007
Last modified: May 1, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents