Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P28724

- DHE4_GIAIN

UniProt

P28724 - DHE4_GIAIN

Protein

NADP-specific glutamate dehydrogenase

Gene
N/A
Organism
Giardia intestinalis (Giardia lamblia)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (01 Dec 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei125 – 1251PROSITE-ProRule annotation

    GO - Molecular functioni

    1. glutamate dehydrogenase (NADP+) activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular amino acid metabolic process Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
    Short name:
    NADP-GDH
    Alternative name(s):
    NADP-dependent glutamate dehydrogenase
    OrganismiGiardia intestinalis (Giardia lamblia)
    Taxonomic identifieri5741 [NCBI]
    Taxonomic lineageiEukaryotaDiplomonadidaHexamitidaeGiardiinaeGiardia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449NADP-specific glutamate dehydrogenasePRO_0000182786Add
    BLAST

    Proteomic databases

    PRIDEiP28724.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP28724.
    SMRiP28724. Positions 4-449.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PfamiPF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000185. Glu_DH. 1 hit.
    PRINTSiPR00082. GLFDHDRGNASE.
    SMARTiSM00839. ELFV_dehydrog. 1 hit.
    [Graphical view]
    PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28724-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPAQTIEELI AVIKQRDGHM TEFRQAVEEV VDSLKVIFER EPKYIPIFER    50
    MLEPERVIIF RVPWMDDAGR INVNRGFRVQ YNSALGPYKG GLRFHPSVNL 100
    SILKFLGFEQ ILKNSLTTLP MGGGKGGSDF DPKGKSDNEV MRFCQSFMTE 150
    LQRHVGADTD VPAGDIGVGA REIGYLYGQY KRLRNEFTGV LTGKNVKWGG 200
    SFIRPEATGY GAVYFLEEMC KDNNTVIRGK NVLLSGSGNV AQFACEKLIQ 250
    LGAKVLTFSD SNGTIVDKDG FNEEKLAHLM YLKNEKRGRV SEFKDKYPSV 300
    AYYEGKKPWE CFEGQMDCIM PCATQNEVSG DDATRLVGLG LKFVAEGANM 350
    PSTAEAVHVY HAKGVMYGPA KASNAGGVSV SGLEMSQNSV RLQWTAEEVD 400
    QKLRGIMRGI FVACRDTAKK YGHPKNYQMG ANIAGFLKVA DSMIEQGCV 449
    Length:449
    Mass (Da):49,766
    Last modified:December 1, 1992 - v1
    Checksum:i5497B35209B549F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti249 – 2491I → L in AAB05400. (PubMed:8587793)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84604 mRNA. Translation: AAA29155.1.
    U47632 Genomic DNA. Translation: AAB05400.1.
    PIRiA42489.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84604 mRNA. Translation: AAA29155.1 .
    U47632 Genomic DNA. Translation: AAB05400.1 .
    PIRi A42489.

    3D structure databases

    ProteinModelPortali P28724.
    SMRi P28724. Positions 4-449.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P28724.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR006095. Glu/Leu/Phe/Val_DH.
    IPR006096. Glu/Leu/Phe/Val_DH_C.
    IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
    IPR014362. Glu_DH.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    Pfami PF00208. ELFV_dehydrog. 1 hit.
    PF02812. ELFV_dehydrog_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000185. Glu_DH. 1 hit.
    PRINTSi PR00082. GLFDHDRGNASE.
    SMARTi SM00839. ELFV_dehydrog. 1 hit.
    [Graphical view ]
    PROSITEi PS00074. GLFV_DEHYDROGENASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia."
      Yee J., Dennis P.P.
      J. Biol. Chem. 267:7539-7544(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
    2. "Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus."
      Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L.
      Parasitology 112:1-12(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-261.

    Entry informationi

    Entry nameiDHE4_GIAIN
    AccessioniPrimary (citable) accession number: P28724
    Secondary accession number(s): Q24961
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 1, 1992
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3