P28724 (DHE4_GIAIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: NADP-specific glutamate dehydrogenase Short name=NADP-GDH EC=1.4.1.4 Alternative name(s): NADP-dependent glutamate dehydrogenase
Organism	Giardia intestinalis (Giardia lamblia)
Taxonomic identifier	5741 [NCBI]
Taxonomic lineage	Eukaryota › Diplomonadida › Hexamitidae › Giardiinae › Giardia

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glutamate dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	NADP-specific glutamate dehydrogenase		PRO_0000182786
Sites
	Active site	125	1	By similarity
Experimental info
	Sequence conflict	249	1	I → L in AAB05400. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P28724 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 5497B35209B549F6	FASTA	449	49,766
	10 20 30 40 50 60 MPAQTIEELI AVIKQRDGHM TEFRQAVEEV VDSLKVIFER EPKYIPIFER MLEPERVIIF 70 80 90 100 110 120 RVPWMDDAGR INVNRGFRVQ YNSALGPYKG GLRFHPSVNL SILKFLGFEQ ILKNSLTTLP 130 140 150 160 170 180 MGGGKGGSDF DPKGKSDNEV MRFCQSFMTE LQRHVGADTD VPAGDIGVGA REIGYLYGQY 190 200 210 220 230 240 KRLRNEFTGV LTGKNVKWGG SFIRPEATGY GAVYFLEEMC KDNNTVIRGK NVLLSGSGNV 250 260 270 280 290 300 AQFACEKLIQ LGAKVLTFSD SNGTIVDKDG FNEEKLAHLM YLKNEKRGRV SEFKDKYPSV 310 320 330 340 350 360 AYYEGKKPWE CFEGQMDCIM PCATQNEVSG DDATRLVGLG LKFVAEGANM PSTAEAVHVY 370 380 390 400 410 420 HAKGVMYGPA KASNAGGVSV SGLEMSQNSV RLQWTAEEVD QKLRGIMRGI FVACRDTAKK 430 440 YGHPKNYQMG ANIAGFLKVA DSMIEQGCV

References

[1]	"Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia." Yee J., Dennis P.P. J. Biol. Chem. 267:7539-7544(1992) [PubMed: 1559991] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[2]	"Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus." Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L. Parasitology 112:1-12(1996) [PubMed: 8587793] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-261.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M84604 mRNA. Translation: AAA29155.1. U47632 Genomic DNA. Translation: AAB05400.1.
PIR	A42489.
3D structure databases
ProteinModelPortal	P28724.
SMR	P28724. Positions 4-449.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. IPR006096. Glu/Leu/Phe/Val_DH_C. IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom. IPR014362. Glu_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00208. ELFV_dehydrog. 1 hit. PF02812. ELFV_dehydrog_N. 1 hit. [Graphical view]
PIRSF	PIRSF000185. Glu_DH. 1 hit.
PRINTS	PR00082. GLFDHDRGNASE.
SMART	SM00839. ELFV_dehydrog. 1 hit. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE4_GIAIN

Accession

Primary (citable) accession number: P28724
Secondary accession number(s): Q24961

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	December 14, 2011
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)