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Protein

NADP-specific glutamate dehydrogenase

Gene
N/A
Organism
Giardia intestinalis (Giardia lamblia)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei125 – 1251PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.4.1.4. 2401.

Names & Taxonomyi

Protein namesi
Recommended name:
NADP-specific glutamate dehydrogenase (EC:1.4.1.4)
Short name:
NADP-GDH
Alternative name(s):
NADP-dependent glutamate dehydrogenase
OrganismiGiardia intestinalis (Giardia lamblia)
Taxonomic identifieri5741 [NCBI]
Taxonomic lineageiEukaryotaDiplomonadidaHexamitidaeGiardiinaeGiardia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449NADP-specific glutamate dehydrogenasePRO_0000182786Add
BLAST

Proteomic databases

PRIDEiP28724.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi184922.XP_001707257.1.

Structurei

3D structure databases

ProteinModelPortaliP28724.
SMRiP28724. Positions 4-449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAQTIEELI AVIKQRDGHM TEFRQAVEEV VDSLKVIFER EPKYIPIFER
60 70 80 90 100
MLEPERVIIF RVPWMDDAGR INVNRGFRVQ YNSALGPYKG GLRFHPSVNL
110 120 130 140 150
SILKFLGFEQ ILKNSLTTLP MGGGKGGSDF DPKGKSDNEV MRFCQSFMTE
160 170 180 190 200
LQRHVGADTD VPAGDIGVGA REIGYLYGQY KRLRNEFTGV LTGKNVKWGG
210 220 230 240 250
SFIRPEATGY GAVYFLEEMC KDNNTVIRGK NVLLSGSGNV AQFACEKLIQ
260 270 280 290 300
LGAKVLTFSD SNGTIVDKDG FNEEKLAHLM YLKNEKRGRV SEFKDKYPSV
310 320 330 340 350
AYYEGKKPWE CFEGQMDCIM PCATQNEVSG DDATRLVGLG LKFVAEGANM
360 370 380 390 400
PSTAEAVHVY HAKGVMYGPA KASNAGGVSV SGLEMSQNSV RLQWTAEEVD
410 420 430 440
QKLRGIMRGI FVACRDTAKK YGHPKNYQMG ANIAGFLKVA DSMIEQGCV
Length:449
Mass (Da):49,766
Last modified:December 1, 1992 - v1
Checksum:i5497B35209B549F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti249 – 2491I → L in AAB05400 (PubMed:8587793).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84604 mRNA. Translation: AAA29155.1.
U47632 Genomic DNA. Translation: AAB05400.1.
PIRiA42489.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84604 mRNA. Translation: AAA29155.1.
U47632 Genomic DNA. Translation: AAB05400.1.
PIRiA42489.

3D structure databases

ProteinModelPortaliP28724.
SMRiP28724. Positions 4-449.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi184922.XP_001707257.1.

Proteomic databases

PRIDEiP28724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi1.4.1.4. 2401.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000185. Glu_DH. 1 hit.
PRINTSiPR00082. GLFDHDRGNASE.
SMARTiSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEiPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia."
    Yee J., Dennis P.P.
    J. Biol. Chem. 267:7539-7544(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  2. "Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus."
    Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L.
    Parasitology 112:1-12(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-261.

Entry informationi

Entry nameiDHE4_GIAIN
AccessioniPrimary (citable) accession number: P28724
Secondary accession number(s): Q24961
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 24, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.