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P28724 (DHE4_GIAIN) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADP-specific glutamate dehydrogenase

Short name=NADP-GDH
EC=1.4.1.4
Alternative name(s):
NADP-dependent glutamate dehydrogenase
OrganismGiardia intestinalis (Giardia lamblia)
Taxonomic identifier5741 [NCBI]
Taxonomic lineageEukaryotaDiplomonadidaHexamitidaeGiardiinaeGiardia

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

L-glutamate + H2O + NADP+ = 2-oxoglutarate + NH3 + NADPH.

Subunit structure

Homohexamer By similarity.

Sequence similarities

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular amino acid metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutamate dehydrogenase (NADP+) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449NADP-specific glutamate dehydrogenase
PRO_0000182786

Sites

Active site1251 By similarity

Experimental info

Sequence conflict2491I → L in AAB05400. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P28724 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 5497B35209B549F6

FASTA44949,766
        10         20         30         40         50         60 
MPAQTIEELI AVIKQRDGHM TEFRQAVEEV VDSLKVIFER EPKYIPIFER MLEPERVIIF 

        70         80         90        100        110        120 
RVPWMDDAGR INVNRGFRVQ YNSALGPYKG GLRFHPSVNL SILKFLGFEQ ILKNSLTTLP 

       130        140        150        160        170        180 
MGGGKGGSDF DPKGKSDNEV MRFCQSFMTE LQRHVGADTD VPAGDIGVGA REIGYLYGQY 

       190        200        210        220        230        240 
KRLRNEFTGV LTGKNVKWGG SFIRPEATGY GAVYFLEEMC KDNNTVIRGK NVLLSGSGNV 

       250        260        270        280        290        300 
AQFACEKLIQ LGAKVLTFSD SNGTIVDKDG FNEEKLAHLM YLKNEKRGRV SEFKDKYPSV 

       310        320        330        340        350        360 
AYYEGKKPWE CFEGQMDCIM PCATQNEVSG DDATRLVGLG LKFVAEGANM PSTAEAVHVY 

       370        380        390        400        410        420 
HAKGVMYGPA KASNAGGVSV SGLEMSQNSV RLQWTAEEVD QKLRGIMRGI FVACRDTAKK 

       430        440 
YGHPKNYQMG ANIAGFLKVA DSMIEQGCV 

« Hide

References

[1]"Isolation and characterization of a NADP-dependent glutamate dehydrogenase gene from the primitive eucaryote Giardia lamblia."
Yee J., Dennis P.P.
J. Biol. Chem. 267:7539-7544(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"Molecular genetic analysis of Giardia intestinalis isolates at the glutamate dehydrogenase locus."
Monis P.T., Mayrhofer G., Andrews R.H., Homan W.L., Limper L., Ey P.L.
Parasitology 112:1-12(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 59-261.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84604 mRNA. Translation: AAA29155.1.
U47632 Genomic DNA. Translation: AAB05400.1.
PIRA42489.

3D structure databases

ProteinModelPortalP28724.
SMRP28724. Positions 4-449.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP28724.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR006095. Glu/Leu/Phe/Val_DH.
IPR006096. Glu/Leu/Phe/Val_DH_C.
IPR006097. Glu/Leu/Phe/Val_DH_dimer_dom.
IPR014362. Glu_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamPF00208. ELFV_dehydrog. 1 hit.
PF02812. ELFV_dehydrog_N. 1 hit.
[Graphical view]
PIRSFPIRSF000185. Glu_DH. 1 hit.
PRINTSPR00082. GLFDHDRGNASE.
SMARTSM00839. ELFV_dehydrog. 1 hit.
[Graphical view]
PROSITEPS00074. GLFV_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDHE4_GIAIN
AccessionPrimary (citable) accession number: P28724
Secondary accession number(s): Q24961
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families