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P28723

- FTHS_SPIOL

UniProt

P28723 - FTHS_SPIOL

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Protein
Formate--tetrahydrofolate ligase
Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.UniRule annotation

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 888ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
10-formyletrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Formyltetrahydrofolate synthetase
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 637636Formate--tetrahydrofolate ligaseUniRule annotation
PRO_0000199418Add
BLAST

Proteomic databases

PRIDEiP28723.
ProMEXiP28723.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP28723.
SMRiP28723. Positions 170-233.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28723-1 [UniParc]FASTAAdd to Basket

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MGGSSKTVRK LEVATPVPAD IDIANAVEPL HIADIAADLN LSPRHYDLYG    50
KYKAKVLLSV LDEVQETQDG YYVVVGGITP TPLGEGKSTT TVGLCQALGA 100
FLDKKVVTCL RQPSQGPTFG IKGGAAGGGY SQVIPMDEFN LHLTGDIHAI 150
TASNNLLAAA IDTRMFHEST QSDKALFNRL CPPNKEGKRT FCNIMHRRLK 200
KLGIDKTNPD DLTPEEVTKF ARLDIDPDSI TWRRVMDVND RFLRKISVGQ 250
GPDEKGMVRE TGFDISVASE IMAVLALTTS LADMRERLGK MVIGNSKAGE 300
PITADDLGLG GALTVLMKDA INPTLMQTLE GTPVLVHAGP FANIAHGNSS 350
IVADKIALKL VGPGGFVVTE AGFGSDIGTE KFMNIKCRYS GLTPQCAIVV 400
ATVRALKMHG GGPQVVAGKP LDRAYLTENV GLVEAGCVNL ARHIINTKAY 450
GSNVVVAINM FSSDTEAELN AVKKAAMDAG AFDAVICTHH AHGGKGAVDL 500
GIAVQKACEN VTQPLRFLYP LDISIKEKIE AIAKSYGAAG VEYSEQAEKK 550
IEMYSKQGFS NLPICMAKTQ YSFSHNAAEK GAPSGFILPI RDVRGSIGAG 600
FIYPLVGTMS TMPGLPTRPC FFDIDLDTTT GKVIGLS 637
Length:637
Mass (Da):67,855
Last modified:January 23, 2007 - v3
Checksum:iAAF08ABA23C54B9E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83940 mRNA. Translation: AAA34046.1.
PIRiA40948.
A43350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M83940 mRNA. Translation: AAA34046.1 .
PIRi A40948.
A43350.

3D structure databases

ProteinModelPortali P28723.
SMRi P28723. Positions 170-233.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P28723.
ProMEXi P28723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and sequencing of the cDNA coding for spinach 10-formyltetrahydrofolate synthetase. Comparisons with the yeast, mammalian, and bacterial proteins."
    Nour J.M., Rabinowitz J.C.
    J. Biol. Chem. 267:16292-16296(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves."
    Nour J.M., Rabinowitz J.C.
    J. Biol. Chem. 266:18363-18369(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-36.
    Tissue: Leaf.

Entry informationi

Entry nameiFTHS_SPIOL
AccessioniPrimary (citable) accession number: P28723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 61 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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