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P28723

- FTHS_SPIOL

UniProt

P28723 - FTHS_SPIOL

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Protein

Formate--tetrahydrofolate ligase

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi81 – 888ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. formate-tetrahydrofolate ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. folic acid-containing compound biosynthetic process Source: InterPro
  2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00193.

Names & Taxonomyi

Protein namesi
Recommended name:
Formate--tetrahydrofolate ligase (EC:6.3.4.3)
Alternative name(s):
10-formyletrahydrofolate synthetase
Short name:
FHS
Short name:
FTHFS
Formyltetrahydrofolate synthetase
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 637636Formate--tetrahydrofolate ligasePRO_0000199418Add
BLAST

Proteomic databases

PRIDEiP28723.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP28723.
SMRiP28723. Positions 170-233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_01543. FTHFS.
InterProiIPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01268. FTHFS. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
PROSITEiPS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28723-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGGSSKTVRK LEVATPVPAD IDIANAVEPL HIADIAADLN LSPRHYDLYG
60 70 80 90 100
KYKAKVLLSV LDEVQETQDG YYVVVGGITP TPLGEGKSTT TVGLCQALGA
110 120 130 140 150
FLDKKVVTCL RQPSQGPTFG IKGGAAGGGY SQVIPMDEFN LHLTGDIHAI
160 170 180 190 200
TASNNLLAAA IDTRMFHEST QSDKALFNRL CPPNKEGKRT FCNIMHRRLK
210 220 230 240 250
KLGIDKTNPD DLTPEEVTKF ARLDIDPDSI TWRRVMDVND RFLRKISVGQ
260 270 280 290 300
GPDEKGMVRE TGFDISVASE IMAVLALTTS LADMRERLGK MVIGNSKAGE
310 320 330 340 350
PITADDLGLG GALTVLMKDA INPTLMQTLE GTPVLVHAGP FANIAHGNSS
360 370 380 390 400
IVADKIALKL VGPGGFVVTE AGFGSDIGTE KFMNIKCRYS GLTPQCAIVV
410 420 430 440 450
ATVRALKMHG GGPQVVAGKP LDRAYLTENV GLVEAGCVNL ARHIINTKAY
460 470 480 490 500
GSNVVVAINM FSSDTEAELN AVKKAAMDAG AFDAVICTHH AHGGKGAVDL
510 520 530 540 550
GIAVQKACEN VTQPLRFLYP LDISIKEKIE AIAKSYGAAG VEYSEQAEKK
560 570 580 590 600
IEMYSKQGFS NLPICMAKTQ YSFSHNAAEK GAPSGFILPI RDVRGSIGAG
610 620 630
FIYPLVGTMS TMPGLPTRPC FFDIDLDTTT GKVIGLS
Length:637
Mass (Da):67,855
Last modified:January 23, 2007 - v3
Checksum:iAAF08ABA23C54B9E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83940 mRNA. Translation: AAA34046.1.
PIRiA40948.
A43350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M83940 mRNA. Translation: AAA34046.1 .
PIRi A40948.
A43350.

3D structure databases

ProteinModelPortali P28723.
SMRi P28723. Positions 170-233.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P28723.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00193 .

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
HAMAPi MF_01543. FTHFS.
InterProi IPR000559. Formate_THF_ligase.
IPR020628. Formate_THF_ligase_CS.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF01268. FTHFS. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
PROSITEi PS00721. FTHFS_1. 1 hit.
PS00722. FTHFS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and sequencing of the cDNA coding for spinach 10-formyltetrahydrofolate synthetase. Comparisons with the yeast, mammalian, and bacterial proteins."
    Nour J.M., Rabinowitz J.C.
    J. Biol. Chem. 267:16292-16296(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves."
    Nour J.M., Rabinowitz J.C.
    J. Biol. Chem. 266:18363-18369(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-36.
    Tissue: Leaf.

Entry informationi

Entry nameiFTHS_SPIOL
AccessioniPrimary (citable) accession number: P28723
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 63 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3