P28723 (FTHS_SPIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Formate--tetrahydrofolate ligase EC=6.3.4.3 Alternative name(s): 10-formyletrahydrofolate synthetase Short name=FHS Short name=FTHFS Formyltetrahydrofolate synthetase
Organism	Spinacia oleracea (Spinach)
Taxonomic identifier	3562 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Amaranthaceae › Spinacia

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion.
Subunit structure	Homodimer.
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 637	636	Formate--tetrahydrofolate ligase		PRO_0000199418
Regions
	Nucleotide binding	81 – 88	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28723 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AAF08ABA23C54B9E	FASTA	637	67,855
	10 20 30 40 50 60 MGGSSKTVRK LEVATPVPAD IDIANAVEPL HIADIAADLN LSPRHYDLYG KYKAKVLLSV 70 80 90 100 110 120 LDEVQETQDG YYVVVGGITP TPLGEGKSTT TVGLCQALGA FLDKKVVTCL RQPSQGPTFG 130 140 150 160 170 180 IKGGAAGGGY SQVIPMDEFN LHLTGDIHAI TASNNLLAAA IDTRMFHEST QSDKALFNRL 190 200 210 220 230 240 CPPNKEGKRT FCNIMHRRLK KLGIDKTNPD DLTPEEVTKF ARLDIDPDSI TWRRVMDVND 250 260 270 280 290 300 RFLRKISVGQ GPDEKGMVRE TGFDISVASE IMAVLALTTS LADMRERLGK MVIGNSKAGE 310 320 330 340 350 360 PITADDLGLG GALTVLMKDA INPTLMQTLE GTPVLVHAGP FANIAHGNSS IVADKIALKL 370 380 390 400 410 420 VGPGGFVVTE AGFGSDIGTE KFMNIKCRYS GLTPQCAIVV ATVRALKMHG GGPQVVAGKP 430 440 450 460 470 480 LDRAYLTENV GLVEAGCVNL ARHIINTKAY GSNVVVAINM FSSDTEAELN AVKKAAMDAG 490 500 510 520 530 540 AFDAVICTHH AHGGKGAVDL GIAVQKACEN VTQPLRFLYP LDISIKEKIE AIAKSYGAAG 550 560 570 580 590 600 VEYSEQAEKK IEMYSKQGFS NLPICMAKTQ YSFSHNAAEK GAPSGFILPI RDVRGSIGAG 610 620 630 FIYPLVGTMS TMPGLPTRPC FFDIDLDTTT GKVIGLS

References

[1]	"Isolation and sequencing of the cDNA coding for spinach 10-formyltetrahydrofolate synthetase. Comparisons with the yeast, mammalian, and bacterial proteins." Nour J.M., Rabinowitz J.C. J. Biol. Chem. 267:16292-16296(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves." Nour J.M., Rabinowitz J.C. J. Biol. Chem. 266:18363-18369(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-36. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M83940 mRNA. Translation: AAA34046.1.
PIR	A40948. A43350.
3D structure databases
ProteinModelPortal	P28723.
SMR	P28723. Positions 170-233.
ModBase	Search...
Proteomic databases
PRIDE	P28723.
ProMEX	P28723.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00193.
Family and domain databases
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_SPIOL

Accession

Primary (citable) accession number: P28723

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 57 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program