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P28723

- FTHS_SPIOL

UniProt

P28723 - FTHS_SPIOL

Protein

Formate--tetrahydrofolate ligase

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 62 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi81 – 888ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. formate-tetrahydrofolate ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. folic acid-containing compound biosynthetic process Source: InterPro
    2. tetrahydrofolate interconversion Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    One-carbon metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00193.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Formate--tetrahydrofolate ligase (EC:6.3.4.3)
    Alternative name(s):
    10-formyletrahydrofolate synthetase
    Short name:
    FHS
    Short name:
    FTHFS
    Formyltetrahydrofolate synthetase
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 637636Formate--tetrahydrofolate ligasePRO_0000199418Add
    BLAST

    Proteomic databases

    PRIDEiP28723.
    ProMEXiP28723.

    Interactioni

    Subunit structurei

    Homodimer.

    Structurei

    3D structure databases

    ProteinModelPortaliP28723.
    SMRiP28723. Positions 170-233.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    HAMAPiMF_01543. FTHFS.
    InterProiIPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF01268. FTHFS. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P28723-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGGSSKTVRK LEVATPVPAD IDIANAVEPL HIADIAADLN LSPRHYDLYG    50
    KYKAKVLLSV LDEVQETQDG YYVVVGGITP TPLGEGKSTT TVGLCQALGA 100
    FLDKKVVTCL RQPSQGPTFG IKGGAAGGGY SQVIPMDEFN LHLTGDIHAI 150
    TASNNLLAAA IDTRMFHEST QSDKALFNRL CPPNKEGKRT FCNIMHRRLK 200
    KLGIDKTNPD DLTPEEVTKF ARLDIDPDSI TWRRVMDVND RFLRKISVGQ 250
    GPDEKGMVRE TGFDISVASE IMAVLALTTS LADMRERLGK MVIGNSKAGE 300
    PITADDLGLG GALTVLMKDA INPTLMQTLE GTPVLVHAGP FANIAHGNSS 350
    IVADKIALKL VGPGGFVVTE AGFGSDIGTE KFMNIKCRYS GLTPQCAIVV 400
    ATVRALKMHG GGPQVVAGKP LDRAYLTENV GLVEAGCVNL ARHIINTKAY 450
    GSNVVVAINM FSSDTEAELN AVKKAAMDAG AFDAVICTHH AHGGKGAVDL 500
    GIAVQKACEN VTQPLRFLYP LDISIKEKIE AIAKSYGAAG VEYSEQAEKK 550
    IEMYSKQGFS NLPICMAKTQ YSFSHNAAEK GAPSGFILPI RDVRGSIGAG 600
    FIYPLVGTMS TMPGLPTRPC FFDIDLDTTT GKVIGLS 637
    Length:637
    Mass (Da):67,855
    Last modified:January 23, 2007 - v3
    Checksum:iAAF08ABA23C54B9E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83940 mRNA. Translation: AAA34046.1.
    PIRiA40948.
    A43350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M83940 mRNA. Translation: AAA34046.1 .
    PIRi A40948.
    A43350.

    3D structure databases

    ProteinModelPortali P28723.
    SMRi P28723. Positions 170-233.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P28723.
    ProMEXi P28723.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00193 .

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    HAMAPi MF_01543. FTHFS.
    InterProi IPR000559. Formate_THF_ligase.
    IPR020628. Formate_THF_ligase_CS.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF01268. FTHFS. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00721. FTHFS_1. 1 hit.
    PS00722. FTHFS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and sequencing of the cDNA coding for spinach 10-formyltetrahydrofolate synthetase. Comparisons with the yeast, mammalian, and bacterial proteins."
      Nour J.M., Rabinowitz J.C.
      J. Biol. Chem. 267:16292-16296(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation, characterization, and structural organization of 10-formyltetrahydrofolate synthetase from spinach leaves."
      Nour J.M., Rabinowitz J.C.
      J. Biol. Chem. 266:18363-18369(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-36.
      Tissue: Leaf.

    Entry informationi

    Entry nameiFTHS_SPIOL
    AccessioniPrimary (citable) accession number: P28723
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 62 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3