Reviewed, UniProtKB/Swiss-Prot P28722 (YHCA_ECOLI)

Names and origin

Protein names

Recommended name:
    Uncharacterized fimbrial chaperone yhcA

Gene names

Name:	yhcA
Ordered Locus Names:	b3215, JW3182

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Periplasm By similarity.
Sequence similarities	Belongs to the periplasmic pilus chaperone family. Contains 1 Ig-like (immunoglobulin-like) domain.

Ontologies

Keywords
Cellular component	Fimbrium Periplasm
Domain	Immunoglobulin domain Signal
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro pilus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
nadE	P18843	1	EBI-1122210,EBI-548960

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 224	205	Uncharacterized fimbrial chaperone yhcA		PRO_0000009295
Experimental info
	Sequence conflict	24 – 58	35	PETSV…TTIVD → LKHQYCWLMKSVVKLVLILR IQMIILHCFTPPLLI Ref.1
	Sequence conflict	204 – 224	21	VEFYP…VTDLK → GRVLPSQPIWLSR in AAA23910. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P28722-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: CFD7E170E7C99684	FASTA	224	25,339
	10 20 30 40 50 60 MLRHITFTVF ITTSMNTLAT GMVPETSVLL VDEKRGEASI NIKNTDDHPS LLYTTIVDLP 70 80 90 100 110 120 ESNKSIRLIP TQPVIRVEAG QVQQVRFLLQ ATVPLQSEEL KRVTFEGIPP KDDKSSRVTV 130 140 150 160 170 180 SIRQDLPVLI HPASLPEERE TWKFLEWRKN GDQIEISNPS NYVVRMTLQF KTLPSGKTGA 190 200 210 220 INKTYFLPHT STTTALTNAT DTKVEFYPAS RYGYRGNKYV TDLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression." Castano I., Flores N., Valle F., Covarrubias A.A., Bolivar F. Mol. Microbiol. 6:2733-2741(1992) [PubMed: 1447980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M74162 Genomic DNA. Translation: AAA23910.1. U18997 Genomic DNA. Translation: AAA58017.1. U00096 Genomic DNA. Translation: AAC76247.1. AP009048 Genomic DNA. Translation: BAE77259.1.
PIR	A65113.
RefSeq	AP_003758.1. NP_417682.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P28722. 11 interactions.
Genome annotation databases
GeneID	947741.
GenomeReviews	Gene locus JW3182 in contig AP009048_GR. Gene locus b3215 in contig U00096_GR.
KEGG	ecj:JW3182. eco:b3215.
Organism-specific databases
EchoBASE	EB1477.
EcoGene	EG11515. yhcA.
CMR	Search...
Phylogenomic databases
HOGENOM	P28722.
OMA	P28722. VALAHAY.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11515-MON.
Family and domain databases
InterPro	IPR008962. PapD-like. IPR016148. Pili_assmbl_chaperone_C_bac. IPR018046. Pili_assmbl_chaperone_CS. IPR016147. Pili_assmbl_chaperone_N_bac. [Graphical view]
Gene3D	G3DSA:2.60.40.360. MSP. 1 hit.
Pfam	PF02753. Pili_assembly_C. 1 hit. PF00345. Pili_assembly_N. 1 hit. [Graphical view]
ProDom	PD001447. Pili_chaperone. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00635. PILI_CHAPERONE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YHCA_ECOLI

Accession

Primary (citable) accession number: P28722
Secondary accession number(s): Q2M8Z7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 73 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)