Reviewed, UniProtKB/Swiss-Prot P28713 (PEPA4_RABIT)

Names and origin

Protein names	Recommended name:     Pepsin II-4     EC=3.4.23.1 Alternative name(s):     Pepsin A
Organism	Oryctolagus cuniculus (Rabbit)
Taxonomic identifier	9986 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Shows particularly broad specificity; although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent.
Catalytic activity	Preferential cleavage: hydrophobic, preferably aromatic, residues in P1 and P1' positions. Cleaves 1-Phe-|-Val-2, 4-Gln-|-His-5, 13-Glu-|-Ala-14, 14-Ala-|-Leu-15, 15-Leu-|-Tyr-16, 16-Tyr-|-Leu-17, 23-Gly-|-Phe-24, 24-Phe-|-Phe-25 and 25-Phe-|-Tyr-26 bonds in the B chain of insulin.
Subcellular location	Secreted.
Developmental stage	Pepsinogens in group I, II, and III where the predominant zymogens at late postnatal stage.
Sequence similarities	Belongs to the peptidase A1 family.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Phosphoprotein Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 59	44	Activation peptide		PRO_0000026032
	Chain	60 – 387	328	Pepsin II-4		PRO_0000026033
Sites
	Active site	93	1	By similarity
	Active site	276	1	By similarity
Amino acid modifications
	Modified residue	129	1	Phosphoserine By similarity
	Disulfide bond	106 ↔ 111		By similarity
	Disulfide bond	267 ↔ 271		By similarity
	Disulfide bond	310 ↔ 343		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28713-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 21ADD07782A89585	FASTA	387	42,052
	10 20 30 40 50 60 MKWLLLLGLL ALSECIVHKV PLVRKKSLRK NLIEKGLLQD YLKTHTPNPA TKYFPKETFA 70 80 90 100 110 120 TVSTESLENY LDAEYFGTIS IGTPPQDFTV IFDTGSSNLW VPSTYCSSLA CALHKRFNPE 130 140 150 160 170 180 DSSTYQGTSE TLSITYGTGS MTGILGYDTV KVGSIEDTNQ IFGLSKTEPG LTFLFAPFDG 190 200 210 220 230 240 ILGLAYPSIS SSDATPVFDN MWNEGLVSQD LFSVYLSSDD EKGSLVMFGG IDSSYYTGSL 250 260 270 280 290 300 NWVPVSYEGY WQITMDSVSI NGETIACADS CQAIVDTGTS LLTGPTSAIS NIQSYIGASK 310 320 330 340 350 360 NLLGENVISC SAIDSLPDIV FTINGIQYPL PASAYILKED DDCTSGLEGM NVDTYTGELW 370 380 ILGDVFIRQY FTVFDRANNQ LGLAAAV

References

[1]

"Structure and development of rabbit pepsinogens. Stage-specific zymogens, nucleotide sequences of cDNAs, molecular evolution, and gene expression during development." Kageyama T., Tanabe K., Koiwai O. J. Biol. Chem. 265:17031-17038(1990) [PubMed: 2129536] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Japanese white. Tissue: Stomach.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB089791 mRNA. Translation: BAC07515.1.
PIR	D38302.
3D structure databases
HSSP	HSSP built from PDB template 1FLH based on UniProtKB P00790.
SMR	P28713. Positions 19-384.
ModBase	Search...
Protein family/group databases
MEROPS	A01.001.
Genome annotation databases
Ensembl	ENSOCUT00000006734; ENSOCUP00000005822; ENSOCUG00000006736; Oryctolagus cuniculus. [Genome view]
Phylogenomic databases
HOVERGEN	P28713.
OMA	EFTIPAS.
Enzyme and pathway databases
BRENDA	3.4.23.1. 255.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR012848. Propep_A1. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF07966. A1_Propeptide. 1 hit. PF00026. Asp. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
PROSITE	PS00141. ASP_PROTEASE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PEPA4_RABIT

Accession

Primary (citable) accession number: P28713
Secondary accession number(s): Q54A53

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	November 3, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)