P28708 (PRR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein kinase PRR1
EC=2.7.11.1

Alternative name(s):
Pheromone response regulator 1

Gene names

Name:	PRR1
Ordered Locus Names:	YKL116C
ORF Names:	YKL516

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	518 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Protein kinase that functions as regulator in the pheromone-induced mating pathway downstream of mitogen-activated protein kinase (MAPK) FUS3. Diminishes transcriptional induction of genes in response to pheromone signaling. Ref.4 Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.9
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 1510 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Pheromone response
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	negative regulation of conjugation with cellular fusion Inferred from mutant phenotype Ref.5. Source: SGD negative regulation of transcription from RNA polymerase II promoter by pheromones Inferred from mutant phenotype Ref.5. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW receptor signaling protein serine/threonine kinase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 518	518	Serine/threonine-protein kinase PRR1		PRO_0000086149
Regions
	Domain	192 – 508	317	Protein kinase
	Nucleotide binding	198 – 206	9	ATP By similarity
Sites
	Active site	354	1	Proton acceptor By similarity
	Binding site	225	1	ATP By similarity
Amino acid modifications
	Modified residue	132	1	Phosphoserine Ref.8 Ref.10 Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P28708 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 4AEB19FAA27671B6	FASTA	518	58,956
	10 20 30 40 50 60 MDEYSSIYSQ PKTPRLKQEG FPDSIGDQHE KALIDENGEE DKKMASTEGT TGDSRSTPLT 70 80 90 100 110 120 VSIPTFENVQ ALPTPMTYTP LSPGNLSMSP IDQSSLNIPK RRSHARLLDD MLSVTQPNQR 130 140 150 160 170 180 VVSELIAPAN LSPQRVVSLP TVTEEALVND SVDSDNYTKE PYFPESSSST EKCDDDIFQG 190 200 210 220 230 240 FLLDHWDRPL LWKKVRPIGS GNFSTVLLYE LMDQSNPKLK QVAVKRLKYP EELSNVEQIN 250 260 270 280 290 300 TSLRYKETLS RLENSLTREL QVLKSLNHPC IVKLLGINNP IFVTSKKPLC DLIIKTPRAL 310 320 330 340 350 360 PPCDMIMSYC PAGDLLAAVM ARNGRLEAWL IQRIFTEVVL AVKYLHENSI IHRDLKLENI 370 380 390 400 410 420 LLKYSFDDIN SFRDSPIYCK QNFIELADFG LCKKIENNEM CTARCGSEDY VSPEILMGVP 430 440 450 460 470 480 YDGHLSDTWA LGVILYSLFE DRLPFDPPPN ASARQRSRAT SHRIARFDWR WYRLSDYKTN 490 500 510 VGKQIVENTL TRKNQRWSIN EIYESPFVKT IADTLSFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases." Jacquier A., Legrain P., Dujon B. Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Analysis of yeast protein kinases using protein chips." Zhu H., Klemic J.F., Chang S., Bertone P., Casamayor A., Klemic K.G., Smith D., Gerstein M., Reed M.A., Snyder M. Nat. Genet. 26:283-289(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[5]	"Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast." Burchett S.A., Scott A., Errede B., Dohlman H.G. J. Biol. Chem. 276:26472-26478(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY. Strain: YAL6B.
[9]	"Global analysis of protein phosphorylation in yeast." Ptacek J., Devgan G., Michaud G., Zhu H., Zhu X., Fasolo J., Guo H., Jona G., Breitkreutz A., Sopko R., McCartney R.R., Schmidt M.C., Rachidi N., Lee S.-J., Mah A.S., Meng L., Stark M.J.R., Stern D.F. , De Virgilio C., Tyers M., Andrews B., Gerstein M., Schweitzer B., Predki P.F., Snyder M. Nature 438:679-684(2005) [PubMed] [Europe PMC] [Abstract] Cited for: CATALYTIC ACTIVITY.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY. Strain: ADR376.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S93804 Genomic DNA. Translation: AAB21999.1. Z28115 Genomic DNA. Translation: CAA81955.1. BK006944 Genomic DNA. Translation: DAA09043.1.
PIR	S27381.
RefSeq	NP_012806.1. NM_001179682.1.
3D structure databases
ProteinModelPortal	P28708.
SMR	P28708. Positions 196-508.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1919N.
IntAct	P28708. 6 interactions.
MINT	MINT-395513.
STRING	4932.YKL116C.
Proteomic databases
PaxDb	P28708.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL116C; YKL116C; YKL116C.
GeneID	853744.
KEGG	sce:YKL116C.
Organism-specific databases
CYGD	YKL116c.
SGD	S000001599. PRR1.
Phylogenomic databases
eggNOG	COG0515.
KO	K08286.
OMA	ARCGSED.
OrthoDB	EOG4DRDN7.
Gene expression databases
Genevestigator	P28708.
GermOnline	YKL116C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR016240. Ser/Thr_kin_YKL116c_prd. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PIRSF	PIRSF000609. Ser/Thr_PK_YKL116c_prd. 1 hit.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974801.

Entry information

Entry name

PRR1_YEAST

Accession

Primary (citable) accession number: P28708
Secondary accession number(s): D6VXH3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program