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P28708 (PRR1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine/threonine-protein kinase PRR1

EC=2.7.11.1
Alternative name(s):
Pheromone response regulator 1
Gene names
Name:PRR1
Ordered Locus Names:YKL116C
ORF Names:YKL516
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length518 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protein kinase that functions as regulator in the pheromone-induced mating pathway downstream of mitogen-activated protein kinase (MAPK) FUS3. Diminishes transcriptional induction of genes in response to pheromone signaling. Ref.4 Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.8

Subcellular location

Cytoplasm Ref.6.

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 518518Serine/threonine-protein kinase PRR1
PRO_0000086149

Regions

Domain192 – 508317Protein kinase
Nucleotide binding198 – 2069ATP By similarity

Sites

Active site3541Proton acceptor By similarity
Binding site2251ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine Ref.12
Modified residue1321Phosphoserine Ref.9 Ref.10 Ref.11

Sequences

Sequence LengthMass (Da)Tools
P28708 [UniParc].

Last modified December 1, 1992. Version 1.
Checksum: 4AEB19FAA27671B6

FASTA51858,956
        10         20         30         40         50         60 
MDEYSSIYSQ PKTPRLKQEG FPDSIGDQHE KALIDENGEE DKKMASTEGT TGDSRSTPLT 

        70         80         90        100        110        120 
VSIPTFENVQ ALPTPMTYTP LSPGNLSMSP IDQSSLNIPK RRSHARLLDD MLSVTQPNQR 

       130        140        150        160        170        180 
VVSELIAPAN LSPQRVVSLP TVTEEALVND SVDSDNYTKE PYFPESSSST EKCDDDIFQG 

       190        200        210        220        230        240 
FLLDHWDRPL LWKKVRPIGS GNFSTVLLYE LMDQSNPKLK QVAVKRLKYP EELSNVEQIN 

       250        260        270        280        290        300 
TSLRYKETLS RLENSLTREL QVLKSLNHPC IVKLLGINNP IFVTSKKPLC DLIIKTPRAL 

       310        320        330        340        350        360 
PPCDMIMSYC PAGDLLAAVM ARNGRLEAWL IQRIFTEVVL AVKYLHENSI IHRDLKLENI 

       370        380        390        400        410        420 
LLKYSFDDIN SFRDSPIYCK QNFIELADFG LCKKIENNEM CTARCGSEDY VSPEILMGVP 

       430        440        450        460        470        480 
YDGHLSDTWA LGVILYSLFE DRLPFDPPPN ASARQRSRAT SHRIARFDWR WYRLSDYKTN 

       490        500        510 
VGKQIVENTL TRKNQRWSIN EIYESPFVKT IADTLSFS 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
Jacquier A., Legrain P., Dujon B.
Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Analysis of yeast protein kinases using protein chips."
Zhu H., Klemic J.F., Chang S., Bertone P., Casamayor A., Klemic K.G., Smith D., Gerstein M., Reed M.A., Snyder M.
Nat. Genet. 26:283-289(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast."
Burchett S.A., Scott A., Errede B., Dohlman H.G.
J. Biol. Chem. 276:26472-26478(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Global analysis of protein phosphorylation in yeast."
Ptacek J., Devgan G., Michaud G., Zhu H., Zhu X., Fasolo J., Guo H., Jona G., Breitkreutz A., Sopko R., McCartney R.R., Schmidt M.C., Rachidi N., Lee S.-J., Mah A.S., Meng L., Stark M.J.R., Stern D.F. expand/collapse author list , De Virgilio C., Tyers M., Andrews B., Gerstein M., Schweitzer B., Predki P.F., Snyder M.
Nature 438:679-684(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: CATALYTIC ACTIVITY.
[9]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S93804 Genomic DNA. Translation: AAB21999.1.
Z28115 Genomic DNA. Translation: CAA81955.1.
BK006944 Genomic DNA. Translation: DAA09043.1.
PIRS27381.
RefSeqNP_012806.1. NM_001179682.1.

3D structure databases

ProteinModelPortalP28708.
SMRP28708. Positions 209-508.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid34019. 75 interactions.
DIPDIP-1919N.
IntActP28708. 7 interactions.
MINTMINT-395513.
STRING4932.YKL116C.

Proteomic databases

MaxQBP28708.
PaxDbP28708.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL116C; YKL116C; YKL116C.
GeneID853744.
KEGGsce:YKL116C.

Organism-specific databases

CYGDYKL116c.
SGDS000001599. PRR1.

Phylogenomic databases

eggNOGCOG0515.
KOK08286.
OMAARCGSED.
OrthoDBEOG722JMF.

Enzyme and pathway databases

BioCycYEAST:G3O-31900-MONOMER.

Gene expression databases

GenevestigatorP28708.

Family and domain databases

InterProIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016240. Ser/Thr_kin_YKL116c_prd.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERPTHR24347. PTHR24347. 1 hit.
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFPIRSF000609. Ser/Thr_PK_YKL116c_prd. 1 hit.
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974801.

Entry information

Entry namePRR1_YEAST
AccessionPrimary (citable) accession number: P28708
Secondary accession number(s): D6VXH3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families