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P28708

- PRR1_YEAST

UniProt

P28708 - PRR1_YEAST

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Protein
Serine/threonine-protein kinase PRR1
Gene
PRR1, YKL116C, YKL516
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Protein kinase that functions as regulator in the pheromone-induced mating pathway downstream of mitogen-activated protein kinase (MAPK) FUS3. Diminishes transcriptional induction of genes in response to pheromone signaling.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei225 – 2251ATP By similarity
Active sitei354 – 3541Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi198 – 2069ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. receptor signaling protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  1. negative regulation of conjugation with cellular fusion Source: SGD
  2. negative regulation of transcription from RNA polymerase II promoter by pheromones Source: SGD
  3. signal transduction by phosphorylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31900-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PRR1 (EC:2.7.11.1)
Alternative name(s):
Pheromone response regulator 1
Gene namesi
Name:PRR1
Ordered Locus Names:YKL116C
ORF Names:YKL516
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL116c.
SGDiS000001599. PRR1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 518518Serine/threonine-protein kinase PRR1
PRO_0000086149Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine1 Publication
Modified residuei132 – 1321Phosphoserine3 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP28708.
PaxDbiP28708.

Expressioni

Gene expression databases

GenevestigatoriP28708.

Interactioni

Protein-protein interaction databases

BioGridi34019. 75 interactions.
DIPiDIP-1919N.
IntActiP28708. 7 interactions.
MINTiMINT-395513.
STRINGi4932.YKL116C.

Structurei

3D structure databases

ProteinModelPortaliP28708.
SMRiP28708. Positions 209-508.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini192 – 508317Protein kinase
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
KOiK08286.
OMAiARCGSED.
OrthoDBiEOG722JMF.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016240. Ser/Thr_kin_YKL116c_prd.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000609. Ser/Thr_PK_YKL116c_prd. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28708-1 [UniParc]FASTAAdd to Basket

« Hide

MDEYSSIYSQ PKTPRLKQEG FPDSIGDQHE KALIDENGEE DKKMASTEGT    50
TGDSRSTPLT VSIPTFENVQ ALPTPMTYTP LSPGNLSMSP IDQSSLNIPK 100
RRSHARLLDD MLSVTQPNQR VVSELIAPAN LSPQRVVSLP TVTEEALVND 150
SVDSDNYTKE PYFPESSSST EKCDDDIFQG FLLDHWDRPL LWKKVRPIGS 200
GNFSTVLLYE LMDQSNPKLK QVAVKRLKYP EELSNVEQIN TSLRYKETLS 250
RLENSLTREL QVLKSLNHPC IVKLLGINNP IFVTSKKPLC DLIIKTPRAL 300
PPCDMIMSYC PAGDLLAAVM ARNGRLEAWL IQRIFTEVVL AVKYLHENSI 350
IHRDLKLENI LLKYSFDDIN SFRDSPIYCK QNFIELADFG LCKKIENNEM 400
CTARCGSEDY VSPEILMGVP YDGHLSDTWA LGVILYSLFE DRLPFDPPPN 450
ASARQRSRAT SHRIARFDWR WYRLSDYKTN VGKQIVENTL TRKNQRWSIN 500
EIYESPFVKT IADTLSFS 518
Length:518
Mass (Da):58,956
Last modified:December 1, 1992 - v1
Checksum:i4AEB19FAA27671B6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S93804 Genomic DNA. Translation: AAB21999.1.
Z28115 Genomic DNA. Translation: CAA81955.1.
BK006944 Genomic DNA. Translation: DAA09043.1.
PIRiS27381.
RefSeqiNP_012806.1. NM_001179682.1.

Genome annotation databases

EnsemblFungiiYKL116C; YKL116C; YKL116C.
GeneIDi853744.
KEGGisce:YKL116C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S93804 Genomic DNA. Translation: AAB21999.1 .
Z28115 Genomic DNA. Translation: CAA81955.1 .
BK006944 Genomic DNA. Translation: DAA09043.1 .
PIRi S27381.
RefSeqi NP_012806.1. NM_001179682.1.

3D structure databases

ProteinModelPortali P28708.
SMRi P28708. Positions 209-508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34019. 75 interactions.
DIPi DIP-1919N.
IntActi P28708. 7 interactions.
MINTi MINT-395513.
STRINGi 4932.YKL116C.

Proteomic databases

MaxQBi P28708.
PaxDbi P28708.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL116C ; YKL116C ; YKL116C .
GeneIDi 853744.
KEGGi sce:YKL116C.

Organism-specific databases

CYGDi YKL116c.
SGDi S000001599. PRR1.

Phylogenomic databases

eggNOGi COG0515.
KOi K08286.
OMAi ARCGSED.
OrthoDBi EOG722JMF.

Enzyme and pathway databases

BioCyci YEAST:G3O-31900-MONOMER.

Miscellaneous databases

NextBioi 974801.

Gene expression databases

Genevestigatori P28708.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016240. Ser/Thr_kin_YKL116c_prd.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000609. Ser/Thr_PK_YKL116c_prd. 1 hit.
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1 and the BAF1 loci and reveals one tRNA gene and several new open reading frames including homologs to RAD2 and kinases."
    Jacquier A., Legrain P., Dujon B.
    Yeast 8:121-132(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: FUNCTION.
  5. "Identification of novel pheromone-response regulators through systematic overexpression of 120 protein kinases in yeast."
    Burchett S.A., Scott A., Errede B., Dohlman H.G.
    J. Biol. Chem. 276:26472-26478(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. Cited for: CATALYTIC ACTIVITY.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-132, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPRR1_YEAST
AccessioniPrimary (citable) accession number: P28708
Secondary accession number(s): D6VXH3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: June 11, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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