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Protein

DNA repair protein rad13

Gene

rad13

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Single-stranded DNA endonuclease involved in excision repair of DNA damaged with UV light, bulky adducts, or cross-linking agents. Essential for the incision step of excision-repair (Probable).Curated

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi30Magnesium 1By similarity1
Metal bindingi77Magnesium 1By similarity1
Metal bindingi777Magnesium 1By similarity1
Metal bindingi779Magnesium 1By similarity1
Metal bindingi798Magnesium 2By similarity1
Metal bindingi800Magnesium 2By similarity1
Metal bindingi849Magnesium 2By similarity1

GO - Molecular functioni

GO - Biological processi

  • nucleotide-excision repair Source: PomBase
  • nucleotide-excision repair, DNA incision, 3'-to lesion Source: PomBase
  • nucleotide-excision repair involved in interstrand cross-link repair Source: PomBase

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-SPO-5696400 Dual Incision in GG-NER
R-SPO-6782135 Dual incision in TC-NER

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein rad13 (EC:3.1.-.-)
Gene namesi
Name:rad13
ORF Names:SPBC3E7.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC3E7.08c
PomBaseiSPBC3E7.08c rad13

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001540361 – 1112DNA repair protein rad13Add BLAST1112

Proteomic databases

MaxQBiP28706
PaxDbiP28706
PRIDEiP28706

PTM databases

iPTMnetiP28706

Interactioni

Protein-protein interaction databases

BioGridi277566, 70 interactors
STRINGi4896.SPBC3E7.08c.1

Structurei

3D structure databases

ProteinModelPortaliP28706
SMRiP28706
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini395 – 414UIMPROSITE-ProRule annotationAdd BLAST20

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 95N-domainAdd BLAST95
Regioni742 – 870I-domainAdd BLAST129

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000214817
InParanoidiP28706
KOiK10846
OMAiAIDSSIW
OrthoDBiEOG092C2ISI
PhylomeDBiP28706

Family and domain databases

InterProiView protein in InterPro
IPR036279 5-3_exonuclease_C_sf
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR003903 UIM_dom
IPR006086 XPG-I_dom
IPR006084 XPG/Rad2
IPR001044 XPG/Rad2_eukaryotes
IPR019974 XPG_CS
IPR006085 XPG_DNA_repair_N
PfamiView protein in Pfam
PF00867 XPG_I, 1 hit
PF00752 XPG_N, 1 hit
PRINTSiPR00853 XPGRADSUPER
PR00066 XRODRMPGMNTG
SMARTiView protein in SMART
SM00279 HhH2, 1 hit
SM00484 XPGI, 1 hit
SM00485 XPGN, 1 hit
SUPFAMiSSF47807 SSF47807, 2 hits
SSF88723 SSF88723, 2 hits
TIGRFAMsiTIGR00600 rad2, 1 hit
PROSITEiView protein in PROSITE
PS50330 UIM, 1 hit
PS00841 XPG_1, 1 hit
PS00842 XPG_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28706-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGVSGLWDIL EPVKRPVKLE TLVNKRLAID ASIWIYQFLK AVRDKEGNQL
60 70 80 90 100
KSSHVVGFFR RICKLLFFGI KPVFVFDGGA PSLKRQTIQK RQARRLDREE
110 120 130 140 150
NATVTANKLL ALQMRHQAML LEENNKKATA LANASVQNER QMPSSMTLDN
160 170 180 190 200
SEIKPVLNQR KNYLKPDPYQ LPEMDVSFDK LGSSYDPRIM SQDELTQYVS
210 220 230 240 250
SFTKIEDINL FDFSNIDFDS ELFQSLPDTD KYSILSAARL RSRLRMGLSS
260 270 280 290 300
EQLSEMFPNR MDFSRFQIER LKERNDLTQR LMDFTGMNEF GPSRVVSEKN
310 320 330 340 350
REYILVKNEG AEGGWALGVI SGSTNNEPII IDDEATKLSS NLIDEDEDEA
360 370 380 390 400
FYDVPLPSRS HSMNPRELVA AKLKEIKENS FSENQQSDEA DYNVTDDLIL
410 420 430 440 450
QLATQQSLEE NKKSKELFSL SASEFDKLNS EKKTFEILST DIPAEDSMNS
460 470 480 490 500
LLNDEENLKL EHVGDVSNDS LAFAEKKHPE NGTSIFMDAL PSASREKKTN
510 520 530 540 550
DLIDPLPFQP MDWGKSIFFE KLKKPTETFM DSKTDIPSEA PDNSKLVEDT
560 570 580 590 600
NLHTINATVN IESDLDAAKP GIENPIISPL LPVKDDEKDL DLRELNPLEP
610 620 630 640 650
FENMKEQADD GTVTNPLNVS SDKAMSVYLL SSENAKDTGD IKSESIDAVL
660 670 680 690 700
PTLETSSPSL SIPTDFQKEA SPNKGAAALS SKVEPEVVEK LLDEEEEEMI
710 720 730 740 750
IRMAEEEKEY DRFVSELNQR HETEEWNQEA FEKRLKELKN QKRSEKRDAD
760 770 780 790 800
EVTQVMIKEC QELLRLFGLP YIVAPQEAEA QCSKLLELKL VDGIVTDDSD
810 820 830 840 850
VFLFGGTRVY RNMFNQNKFV ELYLMDDMKR EFNVNQMDLI KLAHLLGSDY
860 870 880 890 900
TMGLSRVGPV LALEILHEFP GDTGLFEFKK WFQRLSTGHA SKNDVNTPVK
910 920 930 940 950
KRINKLVGKI ILPSEFPNPL VDEAYLHPAV DDSKQSFQWG IPDLDELRQF
960 970 980 990 1000
LMATVGWSKQ RTNEVLLPVI QDMHKKQFVG TQSNLTQFFE GGNTNVYAPR
1010 1020 1030 1040 1050
VAYHFKSKRL ENALSSFKNQ ISNQSPMSEE IQADADAFGE SKGSDELQSR
1060 1070 1080 1090 1100
ILRRKKMMAS KNSSDSDSDS EDNFLASLTP KTNSSSISIE NLPRKTKLST
1110
SLLKKPSKRR RK
Length:1,112
Mass (Da):126,329
Last modified:February 21, 2001 - v2
Checksum:i7ECF4229D5BF4768
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8D → N in CAA47291 (PubMed:8464724).Curated1
Sequence conflicti738 – 743LKNQKR → AQKSKKG in CAA47291 (PubMed:8464724).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X66795 mRNA Translation: CAA47291.1
CU329671 Genomic DNA Translation: CAA19011.1
PIRiS30301
T40382
RefSeqiNP_596095.1, NM_001022011.2

Genome annotation databases

EnsemblFungiiSPBC3E7.08c.1; SPBC3E7.08c.1:pep; SPBC3E7.08c
GeneIDi2541051
KEGGispo:SPBC3E7.08c

Similar proteinsi

Entry informationi

Entry nameiRAD13_SCHPO
AccessioniPrimary (citable) accession number: P28706
Secondary accession number(s): O59728
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: February 21, 2001
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health