P28704 (RXRB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Retinoic acid receptor RXR-beta

Alternative name(s):
MHC class I regulatory element-binding protein H-2RIIBP
Nuclear receptor subfamily 2 group B member 2
Retinoid X receptor beta

Gene names

Name:	Rxrb
Synonyms:	Nr2b2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	520 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Nuclear hormone receptor. Involved in the retinoic acid response pathway. Binds 9-cis retinoic acid (9C-RA). Binds to both, class I regulatory element of major histocompatibility class I genes (CRE) and the estrogen response element (ERE).
Subcellular location	Nucleus.
Tissue specificity	In all tissues tested including brain, thymus spleen and liver.
Domain	Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Sequence similarities	Belongs to the nuclear hormone receptor family. NR2 subfamily. Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Receptor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle cell proliferation Inferred from genetic interaction PubMed 9428411. Source: MGI cellular response to retinoic acid Inferred from direct assay PubMed 18922886. Source: UniProtKB in utero embryonic development Inferred from genetic interaction PubMed 9892670. Source: MGI maternal placenta development Inferred from genetic interaction PubMed 9892670. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 7823919. Source: MGI ventricular cardiac muscle cell differentiation Inferred from mutant phenotype PubMed 9428411. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	9-cis retinoic acid receptor activity Inferred from direct assay Ref.6. Source: MGI sequence-specific DNA binding Inferred from genetic interaction PubMed 7823919. Source: MGI steroid hormone receptor activity Inferred from electronic annotation. Source: InterPro transcription regulatory region DNA binding Inferred from direct assay PubMed 18922886. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.

Isoform Long (identifier: P28704-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P28704-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-110: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 520	520	Retinoic acid receptor RXR-beta		PRO_0000053573
Regions
	DNA binding	192 – 257	66	Nuclear receptor
	Zinc finger	192 – 212	21	NR C4-type
	Zinc finger	228 – 252	25	NR C4-type
	Region	1 – 191	191	Modulating By similarity
	Region	258 – 317	60	Hinge
	Region	318 – 520	203	Ligand-binding By similarity
Natural variations
	Alternative sequence	1 – 110	110	Missing in isoform Short.		VSP_003678
Experimental info
	Sequence conflict	73 – 75	3	SGR → MGP in AAA40081. Ref.4
	Sequence conflict	75 – 77	3	RDS → EFP in AAA37772. Ref.5
	Sequence conflict	433	1	L → M in AAA40081. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 71FAEAEC0D2DB505	FASTA	520	55,866
	10 20 30 40 50 60 MSWATRPPFL PPRHAAGQCG PVGVRKEMHC GVASRWRRRR PWLDPAAAAA AAGEQQALEP 70 80 90 100 110 120 EPGEAGRDGM GDSGRDSRSP DSSSPNPLSQ GIRPSSPPGP PLTPSAPPPP MPPPPLGSPF 130 140 150 160 170 180 PVISSSMGSP GLPPPAPPGF SGPVSSPQIN STVSLPGGGS GPPEDVKPPV LGVRGLHCPP 190 200 210 220 230 240 PPGGPGAGKR LCAICGDRSS GKHYGVYSCE GCKGFFKRTI RKDLTYSCRD NKDCTVDKRQ 250 260 270 280 290 300 RNRCQYCRYQ KCLATGMKRE AVQEERQRGK DKDGDGDGAG GAPEEMPVDR ILEAELAVEQ 310 320 330 340 350 360 KSDQGVEGPG ATGGGGSSPN DPVTNICQAA DKQLFTLVEW AKRIPHFSSL PLDDQVILLR 370 380 390 400 410 420 AGWNELLIAS FSHRSIDVRD GILLATGLHV HRNSAHSAGV GAIFDRVLTE LVSKMRDMRM 430 440 450 460 470 480 DKTELGCLRA IILFNPDAKG LSNPGEVEIL REKVYASLET YCKQKYPEQQ GRFAKLLLRL 490 500 510 520 PALRSIGLKC LEHLFFFKLI GDTPIDTFLM EMLEAPHQLA
	Isoform Short [UniParc]. Checksum: 76353141EEA68AB0	FASTA	410	44,396
	10 20 30 40 50 60 MPPPPLGSPF PVISSSMGSP GLPPPAPPGF SGPVSSPQIN STVSLPGGGS GPPEDVKPPV 70 80 90 100 110 120 LGVRGLHCPP PPGGPGAGKR LCAICGDRSS GKHYGVYSCE GCKGFFKRTI RKDLTYSCRD 130 140 150 160 170 180 NKDCTVDKRQ RNRCQYCRYQ KCLATGMKRE AVQEERQRGK DKDGDGDGAG GAPEEMPVDR 190 200 210 220 230 240 ILEAELAVEQ KSDQGVEGPG ATGGGGSSPN DPVTNICQAA DKQLFTLVEW AKRIPHFSSL 250 260 270 280 290 300 PLDDQVILLR AGWNELLIAS FSHRSIDVRD GILLATGLHV HRNSAHSAGV GAIFDRVLTE 310 320 330 340 350 360 LVSKMRDMRM DKTELGCLRA IILFNPDAKG LSNPGEVEIL REKVYASLET YCKQKYPEQQ 370 380 390 400 410 GRFAKLLLRL PALRSIGLKC LEHLFFFKLI GDTPIDTFLM EMLEAPHQLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse Rxrb gene encoding RXR beta: genomic organization and two mRNA isoforms generated by alternative splicing of transcripts initiated from CpG island promoters." Nagata T., Kanno Y., Ozato K., Taketo M. Gene 142:183-189(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: BALB/c. Tissue: Liver.
[2]	"Sequence of the mouse major histocomaptibility locus class II region." Rowen L., Qin S., Madan A., Loretz C., James R., Dors M., Mix L., Hall J., Lasky S., Hood L. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Limb.
[4]	"Purification, cloning, and RXR identity of the HeLa cell factor with which RAR or TR heterodimerizes to bind target sequences efficiently." Leid M., Kastner P., Lyons R., Nakshatri H., Saunders M., Zacharewsi T., Chen J.Y., Staub A., Garnier J.-M., Mader S., Chambon P. Cell 68:377-395(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 73-520 (ISOFORM LONG). Tissue: Embryo.
[5]	"H-2RIIBP, a member of the nuclear hormone receptor superfamily that binds to both the regulatory element of major histocompatibility class I genes and the estrogen response element." Hamada K., Gleason S.L., Levi B.-Z., Hirschfeld S., Appella E., Ozato K. Proc. Natl. Acad. Sci. U.S.A. 86:8289-8293(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 75-520 (ISOFORM LONG). Tissue: Liver.
[6]	"Characterization of three RXR genes that mediate the action of 9-cis retinoic acid." Mangelsdorf D.J., Borgmeyer U., Heyman R.A., Zhou J.Y., Ong E.S., Oro A.E., Kakizuka A., Evans R.M. Genes Dev. 6:329-344(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ISOFORM SHORT). Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D21831 Genomic DNA. Translation: BAA04858.1. D21831 Genomic DNA. Translation: BAA04859.1. AF100956 Genomic DNA. Translation: AAC69904.1. BC049773 mRNA. Translation: AAH49773.1. M84818 mRNA. Translation: AAA40081.2. M26804 mRNA. Translation: AAA37772.1. X66224 mRNA. Translation: CAA46963.1.
IPI	IPI00226615. IPI00890271.
PIR	A34418. D41727. I84718. S26669.
RefSeq	NP_001192145.1. NM_001205216.1. NP_035436.1. NM_011306.4.
UniGene	Mm.1243.
3D structure databases
ProteinModelPortal	P28704.
SMR	P28704. Positions 189-519.
ModBase	Search...
Protein-protein interaction databases
IntAct	P28704. 1 interaction.
PTM databases
PhosphoSite	P28704.
Proteomic databases
PaxDb	P28704.
PRIDE	P28704.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000116612; ENSMUSP00000112311; ENSMUSG00000039656.
GeneID	20182.
KEGG	mmu:20182.
UCSC	uc008ixs.1. mouse.
Organism-specific databases
CTD	6257.
MGI	MGI:98215. Rxrb.
Phylogenomic databases
eggNOG	NOG327099.
GeneTree	ENSGT00690000101777.
HOGENOM	HOG000260821.
HOVERGEN	HBG005606.
InParanoid	P28704.
KO	K08525.
OrthoDB	EOG4SJ5DV.
Gene expression databases
ArrayExpress	P28704.
Bgee	P28704.
CleanEx	MM_RXRB.
Genevestigator	P28704.
GermOnline	ENSMUSG00000039656. Mus musculus.
Family and domain databases
Gene3D	1.10.565.10. 1 hit. 3.30.50.10. 1 hit.
InterPro	IPR008946. Nucl_hormone_rcpt_ligand-bd. IPR000536. Nucl_hrmn_rcpt_lig-bd_core. IPR000003. Retinoid-X_rcpt/HNF4. IPR001723. Str_hrmn_rcpt. IPR001628. Znf_hrmn_rcpt. IPR013088. Znf_NHR/GATA. [Graphical view]
Pfam	PF00104. Hormone_recep. 1 hit. PF00105. zf-C4. 1 hit. [Graphical view]
PRINTS	PR00545. RETINOIDXR. PR00398. STRDHORMONER. PR00047. STROIDFINGER.
SMART	SM00430. HOLI. 1 hit. SM00399. ZnF_C4. 1 hit. [Graphical view]
SUPFAM	SSF48508. Str_ncl_receptor. 1 hit.
PROSITE	PS00031. NUCLEAR_REC_DBD_1. 1 hit. PS51030. NUCLEAR_REC_DBD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P28704.
ChEMBL	CHEMBL4047.
NextBio	297709.
SOURCE	Search...

Entry information

Entry name

RXRB_MOUSE

Accession

Primary (citable) accession number: P28704
Secondary accession number(s): P33243

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 138 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program