Reviewed,
UniProtKB/Swiss-Prot P28693 (EPHB2_CHICK)
Last modified
February 9, 2010.
Version 103.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ephrin type-B receptor 2 EC=2.7.10.1 Alternative name(s): EPH-like kinase 5 Short name=EK5 Short name=cEK5 | ||||
| Gene names |
| ||||
| Organism | Gallus gallus (Chicken) | ||||
| Taxonomic identifier | 9031 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus |
Protein attributes
| Sequence length | 1004 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Receptor for members of the ephrin-B family. Probably an important component of signal transduction pathways. |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. |
| Subcellular location | |
| Tissue specificity | Wide tissue distribution throughout development and sustained expression in adult brain. The longer form (CEK5+) is specifically expressed in the central nervous system. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. Contains 2 fibronectin type-III domains. Contains 1 protein kinase domain. Contains 1 SAM (sterile alpha motif) domain. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Membrane |
| Coding sequence diversity | Alternative splicing |
| Domain | Repeat Signal Transmembrane |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Receptor Transferase Tyrosine-protein kinase |
| PTM | Glycoprotein |
| Technical term | 3D-structure |
| Gene Ontology (GO) | |
| Cellular component | integral to plasma membrane Inferred from electronic annotation. Source: InterPro |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ephrin receptor activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Long (identifier: P28693-1) Also known as: CEK5+; This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Short (identifier: P28693-2) The sequence of this isoform differs from the canonical sequence as follows: 591-606: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||
Molecule processing | ||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 19 | 19 | Potential | |||||||||||||||||
| Chain | 20 – 1004 | 985 | Ephrin type-B receptor 2 | PRO_0000016829 | ||||||||||||||||
Regions | ||||||||||||||||||||
| Topological domain | 20 – 544 | 525 | Extracellular Potential | |||||||||||||||||
| Transmembrane | 545 – 565 | 21 | Potential | |||||||||||||||||
| Topological domain | 566 – 1004 | 439 | Cytoplasmic Potential | |||||||||||||||||
| Domain | 326 – 427 | 102 | Fibronectin type-III 1 | |||||||||||||||||
| Domain | 433 – 528 | 96 | Fibronectin type-III 2 | |||||||||||||||||
| Domain | 639 – 902 | 264 | Protein kinase | |||||||||||||||||
| Domain | 931 – 995 | 65 | SAM | |||||||||||||||||
| Nucleotide binding | 645 – 653 | 9 | ATP By similarity | |||||||||||||||||
| Motif | 1002 – 1004 | 3 | PDZ-binding Potential | |||||||||||||||||
| Compositional bias | 20 – 321 | 302 | Cys-rich | |||||||||||||||||
Sites | ||||||||||||||||||||
| Active site | 764 | 1 | Proton acceptor By similarity | |||||||||||||||||
| Binding site | 671 | 1 | ATP By similarity | |||||||||||||||||
Amino acid modifications | ||||||||||||||||||||
| Glycosylation | 266 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||
| Glycosylation | 337 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||
| Glycosylation | 429 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||
| Glycosylation | 478 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||
| Glycosylation | 483 | 1 | N-linked (GlcNAc...) Potential | |||||||||||||||||
Natural variations | ||||||||||||||||||||
| Alternative sequence | 591 – 606 | 16 | Missing in isoform Short. | VSP_003018 | ||||||||||||||||
Secondary structure | ||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||
| Helix | 936 – 942 | 7 | ||||||||||||||||||
| Helix | 946 – 948 | 3 | ||||||||||||||||||
| Helix | 949 – 955 | 7 | ||||||||||||||||||
| Helix | 962 – 965 | 4 | ||||||||||||||||||
| Helix | 968 – 974 | 7 | ||||||||||||||||||
| Helix | 981 – 995 | 15 | ||||||||||||||||||
Sequences
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References
| [1] | "Identification of chicken embryo kinase 5, a developmentally regulated receptor-type tyrosine kinase of the Eph family." Pasquale E.B. Cell Regul. 2:523-534(1991) [PubMed: 1664238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT). Tissue: Embryo. |
| [2] | "Five novel avian Eph-related tyrosine kinases are differentially expressed." Sajjadi F.G., Pasquale E.B. Oncogene 8:1807-1813(1993) [PubMed: 8510926] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT). Tissue: Brain. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | M62325 mRNA. Translation: AAA48667.1. Different initiation. | ||||||||||||
| IPI | IPI00589125. IPI00602477. | ||||||||||||
| PIR | A56599. | ||||||||||||
| RefSeq | NP_996834.1. | ||||||||||||
| UniGene | Gga.3405 | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| SMR | P28693. Positions 20-200, 323-439, 324-527, 440-532, 612-910. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| STRING | P28693. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSGALT00000007553; ENSGALP00000007541; ENSGALG00000004741; Gallus gallus. [Genome view] ENSGALT00000007555; ENSGALP00000007543; ENSGALG00000004741; Gallus gallus. [Genome view] | ||||||||||||
| GeneID | 396513. | ||||||||||||
| KEGG | gga:396513. | ||||||||||||
Organism-specific databases | |||||||||||||
| CTD | 396513. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | veNOG06887. | ||||||||||||
| HOGENOM | HBG755340. | ||||||||||||
| HOVERGEN | P28693. | ||||||||||||
| InParanoid | P28693. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 2.7.10.1. 4. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR001090. Ephrin_rcpt_lig-bd. IPR008957. Fibronectin_typ-III-like_fold. IPR003961. FN_III. IPR003962. FnIII_subd. IPR008979. Galactose-bd-like. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001660. SAM. IPR010993. SAM_homology. IPR013761. SAM_type. IPR021129. SAM_type1. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. IPR016257. Tyr_prot_kinase_ephrin_rcpt. IPR001426. Tyr_prot_kinase_rcpt_V_CS. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:2.60.40.30. FN_III-like. 2 hits. G3DSA:1.10.150.50. SAM_type. 1 hit. | ||||||||||||
| PANTHER | PTHR23256. Tyr_prot_kinase. 1 hit. | ||||||||||||
| Pfam | PF01404. Ephrin_lbd. 1 hit. PF00041. fn3. 2 hits. PF00536. SAM_1. 1 hit. [Graphical view] | ||||||||||||
| PIRSF | PIRSF000666. TyrPK_ephrin_receptor. 1 hit. | ||||||||||||
| PRINTS | PR00014. FNTYPEIII. | ||||||||||||
| SMART | SM00615. EPH_lbd. 1 hit. SM00060. FN3. 2 hits. SM00454. SAM. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS01186. EGF_2. 1 hit. Uncertain. PS50853. FN3. 2 hits. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit. PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit. PS50105. SAM_DOMAIN. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Entry information
| Entry name | EPHB2_CHICK | ||||||||
| Accession | Primary (citable) accession number: P28693 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
Relevant documents
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


