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Protein

Ephrin type-B receptor 2

Gene

EPHB2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses (By similarity).By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei671ATPPROSITE-ProRule annotation1
Active sitei764Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi645 – 653ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Receptor, Transferase, Tyrosine-protein kinase

Keywords - Biological processi

Neurogenesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.
ReactomeiR-GGA-2682334. EPH-Ephrin signaling.
R-GGA-373760. L1CAM interactions.
R-GGA-3928663. EPHA-mediated growth cone collapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Ephrin type-B receptor 2 (EC:2.7.10.1)
Alternative name(s):
EPH-like kinase 5
Short name:
EK5
Short name:
cEK5
Gene namesi
Name:EPHB2
Synonyms:CEK5
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini20 – 544ExtracellularSequence analysisAdd BLAST525
Transmembranei545 – 565HelicalSequence analysisAdd BLAST21
Topological domaini566 – 1004CytoplasmicSequence analysisAdd BLAST439

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000001682920 – 1004Ephrin type-B receptor 2Add BLAST985

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi63 ↔ 185By similarity
Disulfide bondi98 ↔ 108By similarity
Glycosylationi266N-linked (GlcNAc...)Sequence analysis1
Glycosylationi337N-linked (GlcNAc...)Sequence analysis1
Glycosylationi429N-linked (GlcNAc...)Sequence analysis1
Glycosylationi478N-linked (GlcNAc...)Sequence analysis1
Glycosylationi483N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP28693.

PTM databases

iPTMnetiP28693.

Expressioni

Tissue specificityi

Wide tissue distribution throughout development and sustained expression in adult brain. The longer form (CEK5+) is specifically expressed in the central nervous system.

Interactioni

Subunit structurei

Heterotetramer upon binding of the ligand. The heterotetramer is composed of an ephrin dimer and a receptor dimer. Oligomerization is probably required to induce biological responses (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Abl1P005205EBI-6725926,EBI-914519From a different organism.

Protein-protein interaction databases

IntActiP28693. 3 interactors.
STRINGi9031.ENSGALP00000007541.

Structurei

Secondary structure

11004
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi936 – 942Combined sources7
Helixi946 – 948Combined sources3
Helixi949 – 955Combined sources7
Helixi962 – 965Combined sources4
Helixi968 – 974Combined sources7
Helixi981 – 995Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGGNMR-A924-998[»]
ProteinModelPortaliP28693.
SMRiP28693.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP28693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 203Eph LBDPROSITE-ProRule annotationAdd BLAST183
Domaini325 – 435Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST111
Domaini436 – 531Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST96
Domaini639 – 902Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini931 – 995SAMPROSITE-ProRule annotationAdd BLAST65

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1002 – 1004PDZ-bindingSequence analysis3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi20 – 321Cys-richAdd BLAST302

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.PROSITE-ProRule annotation
Contains 1 Eph LBD (Eph ligand-binding) domain.PROSITE-ProRule annotation
Contains 2 fibronectin type-III domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP28693.
KOiK05111.
PhylomeDBiP28693.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF07699. Ephrin_rec_like. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P28693-1) [UniParc]FASTAAdd to basket
Also known as: CEK5+

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGPLWFCCLP LALLPLLAAV EETLMDSTTA TAELGWMVHP PSGWEEVSGY
60 70 80 90 100
DENMNTIRTY QVCNVFESSQ NNWLRTKYIR RRGAHRIHVE MKFSVRDCSS
110 120 130 140 150
IPNVPGSCKE TFNLYYYESD FDSATKTFPN WMENPWMKVD TIAADESFSQ
160 170 180 190 200
VDLGGRVMKI NTEVRSFGPV SKNGFYLAFQ DYGGCMSLIA VRVFYRKCPR
210 220 230 240 250
VIQNGAVFQE TLSGAESTSL VAARGTCISN AEEVDVPIKL YCNGDGEWLV
260 270 280 290 300
PIGRCMCRPG YESVENGTVC RGCPSGTFKA SQGDEGCVHC PINSRTTSEG
310 320 330 340 350
ATNCVCRNGY YRADADPVDM PCTTIPSAPQ AVISSVNETS LMLEWTPPRD
360 370 380 390 400
SGGREDLVYN IICKSCGSGR GACTRCGDNV QFAPRQLGLT EPRIYISDLL
410 420 430 440 450
AHTQYTFEIQ AVNGVTDQSP FSPQFASVNI TTNQAAPSAV SIMHQVSRTV
460 470 480 490 500
DSITLSWSQP DQPNGVILDY ELQYYEKNLS ELNSTAVKSP TNTVTVQNLK
510 520 530 540 550
AGTIYVFQVR ARTVAGYGRY SGKMYFQTMT EAEYQTSVQE KLPLIIGSSA
560 570 580 590 600
AGLVFLIAVV VIIIVCNRRR GFERADSEYT DKLQHYTSGH STYRGPPPGL
610 620 630 640 650
GVRLFVMTPG MKIYIDPFTY EDPNEAVREF AKEIDISCVK IEQVIGAGEF
660 670 680 690 700
GEVCSGHLKL PGKREIFVAI KTLKSGYTEK QRRDFLSEAS IMGQFDHPNV
710 720 730 740 750
IHLEGVVTKS SPVMIITEFM ENGSLDSFLR QNDGQFTVIQ LVGMLRGIAA
760 770 780 790 800
GMKYLADMNY VHRDLAARNI LVNSNLVCKV SDFGLSRFLE DDTSDPTYTS
810 820 830 840 850
ALGGKIPIRW TAPEAIQYRK FTSASDVWSY GIVMWEVMSY GERPYWDMTN
860 870 880 890 900
QDVINAIEQD YRLPPPMDCP NALHQLMLDC WQKDRNHRPK FGQIVNTLDK
910 920 930 940 950
MIRNPNSLKA MAPLSSGVNL PLLDRTIPDY TSFNTVDEWL DAIKMSQYKE
960 970 980 990 1000
SFASAGFTTF DIVSQMTVED ILRVGVTLAG HQKKILNSIQ VMRAQMNQIQ

SVEV
Length:1,004
Mass (Da):111,963
Last modified:December 1, 2000 - v3
Checksum:i8D26213970ECC6E0
GO
Isoform Short (identifier: P28693-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     591-606: Missing.

Show »
Length:988
Mass (Da):110,265
Checksum:i510AD71D1DB1EE81
GO

Sequence cautioni

The sequence AAA48667 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003018591 – 606Missing in isoform Short. 2 PublicationsAdd BLAST16

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62325 mRNA. Translation: AAA48667.1. Different initiation.
PIRiA56599.
RefSeqiNP_996834.1. NM_206951.3.
UniGeneiGga.3405.
Gga.7661.

Genome annotation databases

GeneIDi396513.
KEGGigga:396513.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62325 mRNA. Translation: AAA48667.1. Different initiation.
PIRiA56599.
RefSeqiNP_996834.1. NM_206951.3.
UniGeneiGga.3405.
Gga.7661.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1SGGNMR-A924-998[»]
ProteinModelPortaliP28693.
SMRiP28693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28693. 3 interactors.
STRINGi9031.ENSGALP00000007541.

PTM databases

iPTMnetiP28693.

Proteomic databases

PaxDbiP28693.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396513.
KEGGigga:396513.

Organism-specific databases

CTDi2048.

Phylogenomic databases

eggNOGiKOG0196. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233856.
HOVERGENiHBG062180.
InParanoidiP28693.
KOiK05111.
PhylomeDBiP28693.

Enzyme and pathway databases

BRENDAi2.7.10.1. 1306.
ReactomeiR-GGA-2682334. EPH-Ephrin signaling.
R-GGA-373760. L1CAM interactions.
R-GGA-3928663. EPHA-mediated growth cone collapse.

Miscellaneous databases

EvolutionaryTraceiP28693.
PROiP28693.

Family and domain databases

CDDicd00063. FN3. 2 hits.
Gene3Di1.10.150.50. 1 hit.
2.60.120.260. 1 hit.
2.60.40.10. 2 hits.
InterProiIPR027936. Eph_TM.
IPR001090. Ephrin_rcpt_lig-bd_dom.
IPR003961. FN3_dom.
IPR008979. Galactose-bd-like.
IPR009030. Growth_fac_rcpt_.
IPR013783. Ig-like_fold.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR011641. Tyr-kin_ephrin_A/B_rcpt-like.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016257. Tyr_kinase_ephrin_rcpt.
IPR001426. Tyr_kinase_rcpt_V_CS.
[Graphical view]
PfamiPF14575. EphA2_TM. 1 hit.
PF01404. Ephrin_lbd. 1 hit.
PF07699. Ephrin_rec_like. 1 hit.
PF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF00536. SAM_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000666. TyrPK_ephrin_receptor. 1 hit.
PRINTSiPR00109. TYRKINASE.
SMARTiSM00615. EPH_lbd. 1 hit.
SM01411. Ephrin_rec_like. 1 hit.
SM00060. FN3. 2 hits.
SM00454. SAM. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF49265. SSF49265. 1 hit.
SSF49785. SSF49785. 1 hit.
SSF56112. SSF56112. 1 hit.
SSF57184. SSF57184. 2 hits.
PROSITEiPS01186. EGF_2. 1 hit.
PS51550. EPH_LBD. 1 hit.
PS50853. FN3. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS00790. RECEPTOR_TYR_KIN_V_1. 1 hit.
PS00791. RECEPTOR_TYR_KIN_V_2. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEPHB2_CHICK
AccessioniPrimary (citable) accession number: P28693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 2000
Last modified: November 30, 2016
This is version 160 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.