P28686 (NOV_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein NOV

Alternative name(s):
CCN family member 3
Nephroblastoma-overexpressed gene protein

Gene names

Name:	NOV
Synonyms:	CCN3

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Immediate-early protein likely to play a role in cell growth regulation. Its overexpression is associated with tumorigenesis and expression of a N-terminal-truncated version of NOV gene in chicken embryonic fibroblasts (CEF) is sufficient to induce the transformation of CEF in vitro.
Subcellular location	Secreted.
Tissue specificity	Brain and heart, and at a lower level in muscle and intestine, in the embryo. Lung and less so in brain and spleen, in adult chicken.
Developmental stage	MAV1-induced nephroblastomas express a high level of NOV gene whose transcription is normally arrested in adult kidney.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Cellular component	Secreted
Disease	Proto-oncogene
Domain	Signal
Molecular function	Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 351	327	Protein NOV		PRO_0000014413
Regions
	Domain	27 – 101	75	IGFBP N-terminal
	Domain	104 – 170	67	VWFC
	Domain	201 – 246	46	TSP type-1
	Domain	258 – 332	75	CTCK
Amino acid modifications
	Glycosylation	274	1	N-linked (GlcNAc...) Potential
	Disulfide bond	258 ↔ 295		By similarity
	Disulfide bond	275 ↔ 309		By similarity
	Disulfide bond	286 ↔ 325		By similarity
	Disulfide bond	289 ↔ 327		By similarity
	Disulfide bond	294 ↔ 331		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28686 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 1ECB3FA3058C6797	FASTA	351	38,268
	10 20 30 40 50 60 METGGGQGLP VLLLLLLLLR PCEVSGREAA CPRPCGGRCP AEPPRCAPGV PAVLDGCGCC 70 80 90 100 110 120 LVCARQRGES CSPLLPCDES GGLYCDRGPE DGGGAGICMV LEGDNCVFDG MIYRNGETFQ 130 140 150 160 170 180 PSCKYQCTCR DGQIGCLPRC NLGLLLPGPD CPFPRKIEVP GECCEKWVCD PRDEVLLGGF 190 200 210 220 230 240 AMAAYRQEAT LGIDVSDSSA NCIEQTTEWS ACSKSCGMGF STRVTNRNQQ CEMVKQTRLC 250 260 270 280 290 300 MMRPCENEEP SDKKGKKCIQ TKKSMKAVRF EYKNCTSVQT YKPRYCGLCN DGRCCTPHNT 310 320 330 340 350 KTIQVEFRCP QGKFLKKPMM LINTCVCHGN CPQSNNAFFQ PLDPMSSEAK I

References

[1]

"Proviral rearrangements and overexpression of a new cellular gene (nov) in myeloblastosis-associated virus type 1-induced nephroblastomas." Joliot V., Martinerie C., Dambrine G., Plassiart G., Brisac M., Crochet J., Perbal B. Mol. Cell. Biol. 12:10-21(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Brown leghorn.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59284 mRNA. Translation: CAA41975.1.
IPI	IPI00585046.
PIR	S20078.
RefSeq	NP_990599.1. NM_205268.1.
UniGene	Gga.2587.
3D structure databases
ProteinModelPortal	P28686.
ModBase	Search...
Proteomic databases
PaxDb	P28686.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396204.
KEGG	gga:396204.
Organism-specific databases
CTD	4856.
Phylogenomic databases
eggNOG	NOG72934.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	P28686.
OrthoDB	EOG4QZ7M8.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816256.

Entry information

Entry name

NOV_CHICK

Accession

Primary (citable) accession number: P28686

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program