P28676 (GRAN_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Grancalcin

Gene names

Name:	GCA
Synonyms:	GCL

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.
Subunit structure	Homodimer. Interacts with SRI and LCP1. Ref.7 Ref.8 Ref.9 Ref.12
Subcellular location	Cytoplasm. Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side. Note: Primarily cytosolic in the absence of calcium or magnesium ions. Relocates to granules and other membranes in response to elevated calcium and magnesium levels. Ref.1 Ref.7
Tissue specificity	Detected in neutrophils and macrophages (at protein level). Highly expressed in bone marrow. Ref.1
Miscellaneous	This protein has been shown to bind calcium with high affinity.
Sequence similarities	Contains 4 EF-hand domains.
Sequence caution	The sequence BAD93005.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium Metal-binding
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cellular membrane fusion Traceable author statement Ref.7. Source: ProtInc
Cellular_component	cytoplasm Inferred from direct assay. Source: HPA plasma membrane Inferred from direct assay. Source: HPA
Molecular_function	calcium ion binding Inferred from direct assay Ref.12. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 217

217

Grancalcin

PRO_0000073721

Regions

Domain

48 – 83

36

EF-hand 1

Domain

89 – 122

34

EF-hand 2

Domain

119 – 154

36

EF-hand 3

Domain

155 – 180

26

EF-hand 4

Calcium binding

65 – 72

8

1 Ref.7

Calcium binding

132 – 143

12

2 Ref.7

Calcium binding

161 – 172

12

3 Potential

Natural variations

Natural variant

80

1

S → A. Ref.3
Corresponds to variant rs17783344 [ dbSNP | Ensembl ].

VAR_048657

Experimental info

Sequence conflict

166

1

R → D AA sequence Ref.7

Secondary structure

1

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217

Helix Strand Turn

Details...

Helix

54 – 62

9

Helix

63 – 65

3

Helix

70 – 80

11

Turn

81 – 85

5

Helix

91 – 101

11

Beta strand

106 – 109

4

Helix

111 – 131

21

Helix

133 – 135

3

Beta strand

138 – 140

3

Helix

141 – 150

10

Helix

157 – 167

11

Beta strand

169 – 171

3

Beta strand

172 – 174

3

Helix

175 – 193

19

Beta strand

201 – 206

6

Helix

207 – 215

9

Sequences

	Sequence		Length	Mass (Da)
	P28676 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 88CA4DDF835AFFE4	FASTA	217	24,010
	10 20 30 40 50 60 MAYPGYGGGF GNFSIQVPGM QMGQPVPETG PAILLDGYSG PAYSDTYSSA GDSVYTYFSA 70 80 90 100 110 120 VAGQDGEVDA EELQRCLTQS GINGTYSPFS LETCRIMIAM LDRDHTGKMG FNAFKELWAA 130 140 150 160 170 180 LNAWKENFMT VDQDGSGTVE HHELRQAIGL MGYRLSPQTL TTIVKRYSKN GRIFFDDYVA 190 200 210 CCVKLRALTD FFRKRDHLQQ GSANFIYDDF LQGTMAI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of grancalcin, a novel EF-hand calcium-binding protein abundant in neutrophils and monocytes." Boyhan A., Casimir C.M., French J.K., Teahan C.G., Segal A.W. J. Biol. Chem. 267:2928-2933(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY. Tissue: Neutrophil.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain cortex.
[3]	Totoki Y., Toyoda A., Takeda T., Sakaki Y., Tanaka A., Yokoyama S., Ohara O., Nagase T., Kikuno R.F. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ALA-80. Tissue: Brain.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Urinary bladder.
[7]	"Isolation and characterization of grancalcin, a novel 28 kDa EF-hand calcium-binding protein from human neutrophils." Teahan C.G., Totty N.F., Segal A.W. Biochem. J. 286:549-554(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 15-27; 109-125 AND 146-175, SUBUNIT, CALCIUM-BINDING, SUBCELLULAR LOCATION. Tissue: Neutrophil.
[8]	"Biochemical characterization of the penta-EF-hand protein grancalcin and identification of L-plastin as a binding partner." Lollike K., Johnsen A.H., Durussel I., Borregaard N., Cox J.A. J. Biol. Chem. 276:17762-17769(2001) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH LCP1.
[9]	"The PEF family proteins sorcin and grancalcin interact in vivo and in vitro." Hansen C., Tarabykina S., la Cour J.M., Lollike K., Berchtold M.W. FEBS Lett. 545:151-154(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SRI.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]	"Crystal structure of human grancalcin, a member of the penta-EF-hand protein family." Jia J., Han Q., Borregaard N., Lollike K., Cygler M. J. Mol. Biol. 300:1271-1281(2000) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 53-217.
[12]	"Structure of Ca(2+)-loaded human grancalcin." Jia J., Borregaard N., Lollike K., Cygler M. Acta Crystallogr. D 57:1843-1849(2001) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) OF 53-217 IN COMPLEX WITH CALCIUM, SUBUNIT.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M81637 mRNA. Translation: AAA58498.1.
AK312349 mRNA. Translation: BAG35270.1.
AB209768 mRNA. Translation: BAD93005.1. Different initiation.
AC010876 Genomic DNA. Translation: AAX93138.1.
CH471058 Genomic DNA. Translation: EAX11350.1.
BC005214 mRNA. Translation: AAH05214.1.

IPI

IPI00004524.

PIR

A42578.

RefSeq

NP_036330.1. NM_012198.3.

UniGene

Hs.377894.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1F4O	X-ray	2.50	A/B	53-217	[»]
1F4Q	X-ray	1.90	A/B	53-217	[»]
1K94	X-ray	1.70	A/B	53-217	[»]
1K95	X-ray	1.90	A	53-217	[»]

ProteinModelPortal

P28676.

ModBase

Search...

Protein-protein interaction databases

IntAct

P28676. 4 interactions.

MINT

MINT-267888.

STRING

9606.ENSP00000394842.

PTM databases

PhosphoSite

P28676.

Polymorphism databases

DMDM

1170014.

Proteomic databases

PaxDb

P28676.

PeptideAtlas

P28676.

PRIDE

P28676.

Protocols and materials databases

DNASU

25801.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000437150; ENSP00000394842; ENSG00000115271.

GeneID

25801.

KEGG

hsa:25801.

UCSC

uc002ucg.3. human.

Organism-specific databases

CTD

25801.

GeneCards

GC02P163164.

HGNC

HGNC:15990. GCA.

HPA

HPA035033.
HPA035034.

MIM

607030. gene.

neXtProt

NX_P28676.

PharmGKB

PA28602.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG298587.

HOGENOM

HOG000231982.

HOVERGEN

HBG004492.

InParanoid

P28676.

OMA

HHELSQA.

PhylomeDB

P28676.

Gene expression databases

ArrayExpress

P28676.

Bgee

P28676.

CleanEx

HS_GCA.

Genevestigator

P28676.

GermOnline

ENSG00000115271. Homo sapiens.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]

Pfam

PF13405. EF_hand_4. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 2 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

GCA. human.

EvolutionaryTrace

P28676.

GenomeRNAi

25801.

NextBio

47003.

SOURCE

Search...

Entry information

Entry name

GRAN_HUMAN

Accession

Primary (citable) accession number: P28676
Secondary accession number(s): B2R5X3, Q53TB5, Q59EP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 124 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.