Reviewed, UniProtKB/Swiss-Prot P28669 (SYYM_PODAN)

Names and origin

Protein names

Recommended name:
    Tyrosyl-tRNA synthetase, mitochondrial
    EC=6.1.1.1
Alternative name(s):
    Tyrosine--tRNA ligase
      Short name=TyrRS

Gene names

Name:

YTS1

Organism

Podospora anserina

Taxonomic identifier

5145 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Lasiosphaeriaceae › Podospora

Protein attributes

Sequence length	640 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Has both an aminoacyl-tRNA synthetase activity and is involved in the splicing of group I introns.
Catalytic activity	ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr).
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis mRNA processing
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Gene Ontology (GO)
Biological process	mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW tyrosyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW tyrosine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 640		Tyrosyl-tRNA synthetase, mitochondrial		PRO_0000035834
Regions
	Motif	105 – 114	10	"HIGH" region
	Motif	322 – 326	5	"KMSKS" region
Sites
	Binding site	325	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28669-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 1DBA1C469FF5E3C6	FASTA	640	72,406
	10 20 30 40 50 60 MSMSRGSVCR RCLLTMKSMA GGGPTSTYAQ QRGKKTWHGP KYQAKIDQAQ ADWEERAEKI 70 80 90 100 110 120 KKGEIQHTWD MFVERGYVKD TAGSHETIRK LMLHKRIGAY TGIDPTAPSL HIGHLLPLMP 130 140 150 160 170 180 IFWMYMHGYA GYTLIGGATA KIGDPTDRLV SRTPLKRTDL TMNLTKIHYQ LKALWMNVEE 190 200 210 220 230 240 QARRRGFEKD WAWKRAVVNN STWWNSLPLI EVLKRLGDSM RMGPLLSRDT VKNKMSKGDG 250 260 270 280 290 300 MSFSEFTYPL MQGWDWWHMY QANGIQMQIG GSDQYGNIVT GVETVKVVRD NEPDPAKKIE 310 320 330 340 350 360 GGPFNDPVGF TVPLLTDSAG VKFGKSAGNA FLDKFQTSEF DLYGYFVRRS DQEVEKLLKL 370 380 390 400 410 420 FTFLPMENIN EAMKIHSENP ARRVAQHLLA FEVVGLVHGM NAAHRTALNH QARYGKQIDI 430 440 450 460 470 480 PGVTLRMPKA ATEDTPPSIL DAPKMDMQLP ESLIMGKSIG RILYAAGLAK SASEGHRLAT 490 500 510 520 530 540 QQGAYIGAMP GHKRTEDNKV MDYSQLSFTP IKLWFPQETR NYLIDGKLLI LRKGKVQIRV 550 560 570 580 590 600 IEMVSDEEWK ESGQTYPGEP GTGALRMLRQ QLKMLKSGML TPDEVKANLK NHVEEEAPPP 610 620 630 640 GFMKFPDQDS YAIRRATQEL MDEIHQKEVG GDSPREERRE

References

[1]

"The mitochondrial tyrosyl-tRNA synthetase of Podospora anserina is a bifunctional enzyme active in protein synthesis and RNA splicing." Kaempfer U., Kueck U., Cherniak A.D., Lambowitz A.M. Mol. Cell. Biol. 12:499-511(1992) [PubMed: 1531084] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S-.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54981 Genomic DNA. Translation: CAA38725.1.
PIR	A42019.
3D structure databases
SMR	P28669. Positions 40-414, 69-544.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.1.1.1. 142554.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002305. aa-tRNA-synth_Ib. IPR014729. Rossmann-like_a/b/a_fold. IPR002307. Tyr-tRNA-synth_Ib_bac/mito. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11766. Tyr_tRNA-synt_1b. 1 hit.
Pfam	PF00579. tRNA-synt_1b. 1 hit. [Graphical view]
PRINTS	PR01040. TRNASYNTHTYR.
TIGRFAMs	TIGR00234. tyrS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYYM_PODAN

Accession

Primary (citable) accession number: P28669

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	February 9, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)