Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional glutamate/proline--tRNA ligase

Gene

GluProRS

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.By similarity

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei217ATPBy similarity1
Binding sitei404ATPBy similarity1
Binding sitei1353L-prolineBy similarity1
Binding sitei1443L-prolineBy similarity1
Binding sitei1476ATPBy similarity1
Metal bindingi1648ZincBy similarity1
Metal bindingi1653ZincBy similarity1
Metal bindingi1695ZincBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi438 – 442ATPBy similarity5
Nucleotide bindingi1353 – 1355ATPBy similarity3
Nucleotide bindingi1364 – 1365ATPBy similarity2
Nucleotide bindingi1438 – 1441ATPBy similarity4

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • glutamate-tRNA ligase activity Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • proline-tRNA ligase activity Source: FlyBase
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

  • glutamyl-tRNA aminoacylation Source: FlyBase
  • prolyl-tRNA aminoacylation Source: FlyBase

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, Multifunctional enzyme, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional glutamate/proline--tRNA ligase
Alternative name(s):
Bifunctional aminoacyl-tRNA synthetase
Including the following 2 domains:
Glutamate--tRNA ligase (EC:6.1.1.17)
Alternative name(s):
Glutamyl-tRNA synthetase1 Publication
Proline--tRNA ligase (EC:6.1.1.15By similarity)
Alternative name(s):
Prolyl-tRNA synthetase
Short name:
ProRS
Gene namesi
Name:GluProRS1 PublicationImported
Synonyms:Aats-gluproImported
ORF Names:CG5394Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0005674 GluProRS

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001197411 – 1714Bifunctional glutamate/proline--tRNA ligaseAdd BLAST1714

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1110Phosphoserine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP28668
PRIDEiP28668

PTM databases

iPTMnetiP28668

Expressioni

Gene expression databases

BgeeiFBgn0005674
ExpressionAtlasiP28668 baseline and differential
GenevisibleiP28668 DM

Interactioni

Subunit structurei

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.By similarity

Protein-protein interaction databases

BioGridi67770, 47 interactors
IntActiP28668, 10 interactors
STRINGi7227.FBpp0083898

Structurei

3D structure databases

ProteinModelPortaliP28668
SMRiP28668
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini744 – 800WHEP-TRS 1Add BLAST57
Domaini816 – 872WHEP-TRS 2Add BLAST57
Domaini890 – 946WHEP-TRS 3Add BLAST57
Domaini969 – 1025WHEP-TRS 4Add BLAST57
Domaini1044 – 1100WHEP-TRS 5Add BLAST57
Domaini1118 – 1174WHEP-TRS 6Add BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni170 – 754Glutamate--tRNA ligaseAdd BLAST585
Regioni1207 – 1714Proline--tRNA ligaseAdd BLAST508
Regioni1322 – 1324L-proline bindingBy similarity3

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi209 – 220"HIGH" regionAdd BLAST12
Motifi438 – 442"KMSKS" region5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1174 – 1180Poly-Gly7

Sequence similaritiesi

In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1147 Eukaryota
KOG4163 Eukaryota
COG0008 LUCA
COG0442 LUCA
GeneTreeiENSGT00550000074815
InParanoidiP28668
KOiK14163
OMAiVAMLHIK
OrthoDBiEOG091G04TK
PhylomeDBiP28668

Family and domain databases

CDDicd00778 ProRS_core_arch_euk, 1 hit
Gene3Di2.40.240.10, 3 hits
3.30.110.30, 1 hit
3.40.50.620, 1 hit
3.40.50.800, 1 hit
HAMAPiMF_02076 Glu_tRNA_synth_type2, 1 hit
MF_01571 Pro_tRNA_synth_type3, 1 hit
InterProiView protein in InterPro
IPR002314 aa-tRNA-synt_IIb
IPR001412 aa-tRNA-synth_I_CS
IPR006195 aa-tRNA-synth_II
IPR004154 Anticodon-bd
IPR036621 Anticodon-bd_dom_sf
IPR004526 Glu-tRNA-synth_arc/euk
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR036282 Glutathione-S-Trfase_C_sf
IPR004046 GST_C
IPR004499 Pro-tRNA-ligase_IIa_arc-type
IPR016061 Pro-tRNA_ligase_II_C
IPR017449 Pro-tRNA_synth_II
IPR033721 ProRS_core_arch_euk
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
IPR009068 S15_NS1_RNA-bd
IPR000738 WHEP-TRS_dom
PfamiView protein in Pfam
PF14497 GST_C_3, 1 hit
PF03129 HGTP_anticodon, 1 hit
PF09180 ProRS-C_1, 1 hit
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PF00587 tRNA-synt_2b, 1 hit
PF00458 WHEP-TRS, 6 hits
PRINTSiPR00987 TRNASYNTHGLU
SMARTiView protein in SMART
SM00946 ProRS-C_1, 1 hit
SM00991 WHEP-TRS, 6 hits
SUPFAMiSSF47060 SSF47060, 6 hits
SSF47616 SSF47616, 1 hit
SSF50715 SSF50715, 1 hit
SSF64586 SSF64586, 1 hit
TIGRFAMsiTIGR00463 gltX_arch, 1 hit
TIGR00408 proS_fam_I, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit
PS50862 AA_TRNA_LIGASE_II, 1 hit
PS00762 WHEP_TRS_1, 6 hits
PS51185 WHEP_TRS_2, 6 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P28668-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSIKLKANLN NPPISGLATA HLINGTVPVE IVWSKEETSL QFPDNRLLVC
60 70 80 90 100
HSNNDVLRAL ARAAPDYKLY GETAIERTQI DHWLSFSLTC EDDISWALSF
110 120 130 140 150
LDKSIAPVTY LVANKLTIAD FALFNEMHSR YEFLAAKGIP QHVQRWYDLI
160 170 180 190 200
TAQPLIQKVL QSLPEDAKVK RSPQSSKEQT PAKTGERKQE GKFVDLPGAE
210 220 230 240 250
MGKVVVRFPP EASGYLHIGH AKAALLNQYY ALAFQGTLIM RFDDTNPAKE
260 270 280 290 300
TVEFENVILG DLEQLQIKPD VFTHTSNYFD LMLDYCVRLI KESKAYVDDT
310 320 330 340 350
PPEQMKLERE QRVESANRSN SVEKNLSLWE EMVKGSEKGQ KYCVRAKIDM
360 370 380 390 400
SSPNGCMRDP TIYRCKNEPH PRTGTKYKVY PTYDFACPIV DAIENVTHTL
410 420 430 440 450
RTTEYHDRDD QFYWFIDALK LRKPYIWSYS RLNMTNTVLS KRKLTWFVDS
460 470 480 490 500
GLVDGWDDPR FPTVRGIIRR GMTVEGLKEF IIAQGSSKSV VFMNWDKIWA
510 520 530 540 550
FNKKVIDPIA PRYTALEKEK RVIVNVAGAK VERIQVSVHP KDESLGKKTV
560 570 580 590 600
LLGPRIYIDY VDAEALKEGE NATFINWGNI LIRKVNKDAS GNITSVDAAL
610 620 630 640 650
NLENKDFKKT LKLTWLAVED DPSAYPPTFC VYFDNIISKA VLGKDEDFKQ
660 670 680 690 700
FIGHKTRDEV PMLGDPELKK CKKGDIIQLQ RRGFFKVDVA YAPPSGYTNV
710 720 730 740 750
PSPIVLFSIP DGHTKDVPTS GLKVNAPDAK ATKKASSPVS SSGQASELDS
760 770 780 790 800
QISQQGDLVR DLKSKKAAKD QIDVAVKKLL ALKADYKSAT GKDWKPGQTS
810 820 830 840 850
ASSAPVPAAS SSSANDAVSV NASIVKQGDL VRDLKGKKAS KPEIDAAVKT
860 870 880 890 900
LLELKAQYKT LTGQDWKPGT VPTTAAPSAS AAPSVGVNDS VAQILSQITA
910 920 930 940 950
QGDKVRELKS AKADKATVDA AVKTLLSLKA DYKAATGSDW KPGTTAPAPA
960 970 980 990 1000
AAPVKVKQEK NPDPASVLTV NTLLNKIAQQ GDKIRQLKSA KSEKSLVEAE
1010 1020 1030 1040 1050
VKLLLALKTD YKSLTGQEWK PGTVAPAPTT VNVIDLTGGD SGSDVGSVLS
1060 1070 1080 1090 1100
KIQAQGDKIR KLKSEKAAKN VIDPEVKTLL ALKGEYKTLS GKDWTPDAKS
1110 1120 1130 1140 1150
EPAVVKKEAS PVSMASPAKD ELTQEINAQG EKVRAAKGNK AAKEVIDAEV
1160 1170 1180 1190 1200
AKLLALKAKY KEVTGTDFPV AGRGGGGGGG SAKKAPKEAQ PKPAKPVKKE
1210 1220 1230 1240 1250
PAADASGAVK KQTRLGLEAT KEDNLPDWYS QVITKGEMIE YYDVSGCYIL
1260 1270 1280 1290 1300
RQWSFAIWKA IKTWFDAEIT RMGVKECYFP IFVSKAVLEK EKTHIADFAP
1310 1320 1330 1340 1350
EVAWVTKSGD SDLAEPIAVR PTSETVMYPA YAKWVQSYRD LPIRLNQWNN
1360 1370 1380 1390 1400
VVRWEFKQPT PFLRTREFLW QEGHTAFADK EEAAKEVLDI LDLYALVYTH
1410 1420 1430 1440 1450
LLAIPVVKGR KTEKEKFAGG DYTTTVEAFI SASGRAIQGA TSHHLGQNFS
1460 1470 1480 1490 1500
KMFEIVYEDP ETQQKKYVYQ NSWGITTRTI GVMIMVHADN QGLVLPPHVA
1510 1520 1530 1540 1550
CIQAIVVPCG ITVNTKDDER AQLLDACKAL EKRLVGGGVR CEGDYRDNYS
1560 1570 1580 1590 1600
PGWKFNHWEL KGVPLRLEVG PKDLKAQQLV AVRRDTVEKI TIPLADVEKK
1610 1620 1630 1640 1650
IPALLETIHE SMLNKAQEDM TSHTKKVTNW TDFCGFLEQK NILLAPFCGE
1660 1670 1680 1690 1700
ISCEDKIKAD SARGEEAEPG APAMGAKSLC IPFDQPAPIA ASDKCINPSC
1710
TNKPKFYTLF GRSY
Length:1,714
Mass (Da):189,412
Last modified:March 1, 2004 - v2
Checksum:i3F8CF3DB128765A8
GO
Isoform B (identifier: P28668-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-718: Missing.
     719-732: TSGLKVNAPDAKAT → MLNYLACGSLSSTS

Note: No experimental confirmation available.
Show »
Length:996
Mass (Da):107,835
Checksum:i0E0D298DF6D006C9
GO

Sequence cautioni

The sequence AAN71400 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti102 – 106DKSIA → TSPLP (PubMed:1756734).Curated5
Sequence conflicti102 – 106DKSIA → TSPLP (PubMed:9063462).Curated5
Sequence conflicti233 – 234AF → VC (PubMed:1756734).Curated2
Sequence conflicti233 – 234AF → VC (PubMed:9063462).Curated2
Sequence conflicti341 – 345KYCVR → NTACA (PubMed:1756734).Curated5
Sequence conflicti341 – 345KYCVR → NTACA (PubMed:9063462).Curated5
Sequence conflicti583R → K (PubMed:1756734).Curated1
Sequence conflicti583R → K (PubMed:9063462).Curated1
Sequence conflicti692A → L (PubMed:1756734).Curated1
Sequence conflicti692A → L (PubMed:9063462).Curated1
Sequence conflicti753S → T (PubMed:1756734).Curated1
Sequence conflicti753S → T (PubMed:9063462).Curated1
Sequence conflicti802S → T (PubMed:1756734).Curated1
Sequence conflicti802S → T (PubMed:9063462).Curated1
Sequence conflicti873T → P (PubMed:1756734).Curated1
Sequence conflicti873T → P (PubMed:9063462).Curated1
Sequence conflicti887V → G (PubMed:1756734).Curated1
Sequence conflicti887V → G (PubMed:9063462).Curated1
Sequence conflicti1035D → G in AAN71400 (PubMed:12537569).Curated1
Sequence conflicti1202Missing (PubMed:1756734).Curated1
Sequence conflicti1202Missing (PubMed:9063462).Curated1
Sequence conflicti1461E → EK (PubMed:1756734).Curated1
Sequence conflicti1461E → EK (PubMed:9063462).Curated1
Sequence conflicti1587V → G (PubMed:1756734).Curated1
Sequence conflicti1587V → G (PubMed:9063462).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096091 – 718Missing in isoform B. 1 PublicationAdd BLAST718
Alternative sequenceiVSP_009610719 – 732TSGLK…DAKAT → MLNYLACGSLSSTS in isoform B. 1 PublicationAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74104 mRNA Translation: AAA28594.1
U59923 Genomic DNA Translation: AAC47469.1
AE014297 Genomic DNA Translation: AAF56211.1
AE014297 Genomic DNA Translation: AAN13964.1
AY058703 mRNA Translation: AAL13932.1
BT001645 mRNA Translation: AAN71400.1 Different initiation.
PIRiS18644
RefSeqiNP_524471.2, NM_079747.3 [P28668-1]
NP_732925.1, NM_170103.2 [P28668-2]
UniGeneiDm.1304

Genome annotation databases

EnsemblMetazoaiFBtr0084511; FBpp0083898; FBgn0005674 [P28668-1]
FBtr0084512; FBpp0083899; FBgn0005674 [P28668-2]
GeneIDi42834
KEGGidme:Dmel_CG5394

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSYEP_DROME
AccessioniPrimary (citable) accession number: P28668
Secondary accession number(s): Q8IGR4
, Q8IMX9, Q95TL3, Q9VCF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: March 1, 2004
Last modified: May 23, 2018
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health