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P28666 (MUG2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Murinoglobulin-2

Short name=MuG2
Gene names
Name:Mug2
Synonyms:Mug-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1451 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.

Subunit structure

Monomer.

Subcellular location

Secreted.

Tissue specificity

Plasma.

Sequence similarities

Belongs to the protease inhibitor I39 (alpha-2-macroglobulin) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   DomainBait region
Signal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
Glycoprotein
Thioester bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: InterPro

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 By similarity
Chain28 – 14511424Murinoglobulin-2
PRO_0000000060

Regions

Region677 – 73458Bait region

Amino acid modifications

Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation3131N-linked (GlcNAc...) Potential
Glycosylation5001N-linked (GlcNAc...) Potential
Glycosylation7491N-linked (GlcNAc...) Potential
Glycosylation7761N-linked (GlcNAc...) Potential
Glycosylation8711N-linked (GlcNAc...) Potential
Glycosylation14011N-linked (GlcNAc...) Potential
Disulfide bond48 ↔ 86 By similarity
Disulfide bond251 ↔ 276 By similarity
Disulfide bond269 ↔ 288 By similarity
Disulfide bond461 ↔ 555 By similarity
Disulfide bond587 ↔ 773 By similarity
Disulfide bond634 ↔ 680 By similarity
Disulfide bond849 ↔ 885 By similarity
Disulfide bond923 ↔ 1274 By similarity
Disulfide bond1081 ↔ 1104 By similarity
Disulfide bond1298 ↔ 1444 By similarity
Cross-link974 ↔ 977Isoglutamyl cysteine thioester (Cys-Gln) By similarity

Experimental info

Sequence conflict3861A → T in AAA73041. Ref.1
Sequence conflict8941E → A in AAA73041. Ref.1
Sequence conflict11811V → I in AAA73041. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P28666 [UniParc].

Last modified June 28, 2011. Version 2.
Checksum: 8DB472A6C033C35D

FASTA1,451162,382
        10         20         30         40         50         60 
MWKSRRAQLC LFSVLLAFLP SASSLNGDSK YMVLVPSQLY TETPEKICLH LYHLNETVTV 

        70         80         90        100        110        120 
TASLVSQTGR RNLFDELVVD KDLFQCVSFI IPTLNSPDEE EFLYVDIKGP THEFSKRNAV 

       130        140        150        160        170        180 
LVKNKESVVF VQTDKPVYKP GQSVKFRVVS MDKTLRPLNE LLPLAYIEDP KKNRIMQWRD 

       190        200        210        220        230        240 
IKTENGLKQM SFSLAAEPIQ GPYKIVVHKQ SGVKEEHSFT VMEFVLPRFN VDLKVPNAIS 

       250        260        270        280        290        300 
VNDEVLQVTV CGKYTYGKPV PGQVKISICH ETEAGCKEVN SKLDNNGCST QEVNITELQS 

       310        320        330        340        350        360 
KKRNYEVQLF HVNATVTEEG TGLEFNGYGT TKIERITNKL IFLKADSHFR HGIPFFVKVR 

       370        380        390        400        410        420 
LVDIKGDPIP NERVFIKAQV LGYTSATTTD QHGLAKFSID TAGFSGSSLH IKVNHKGKDS 

       430        440        450        460        470        480 
CYFFYCMEER YASAEHVAYA VYSLSKSYIY LVKETSSILP CNQIHTVQAH FILKGDLGVL 

       490        500        510        520        530        540 
KELVFYYLVM AQGSIIQTGN HTHQVEPGEA PVKGNFDLEI PVEFSMAPMA KMLIYTILPD 

       550        560        570        580        590        600 
GEVIADSVNF EIEKCLRNKV DLSFSSSQSL PASQTRLQVT ASPQSLCGLR AVDQSVLLLK 

       610        620        630        640        650        660 
PEDELSPSWI YNLPGMQHNK FIPSSSLSED REDCILYSSW VAEKHTDWVP HGREKDVYRY 

       670        680        690        700        710        720 
VEDMDLKAFT NLKIKLPKIC FDSAPMSGPR GKFDLAFSSE VSGTLQKGSS KRPQPEEPPR 

       730        740        750        760        770        780 
EDPPPKDPLA ETIRKYFPET WVWDIVTVNS TGVAEVEMTV PDTITEWKAG ALCLSNDTGL 

       790        800        810        820        830        840 
GLSSVVPLQA FQPFFVEVSL PYSVVRGEAF MLKATVMNYL PTSMRMSVQL EASPDFTAVP 

       850        860        870        880        890        900 
VGDDHDSYCL SANGRHTSSW LVTPKSLGNV NFSVSVEAQQ SSEPCGSEVA TVPETGRKDT 

       910        920        930        940        950        960 
VVKVLIVEPE GIKQEHTFNS LFCASDAEIS EKMSLVLPPT VVKDSARAHF SVMGDILSSA 

       970        980        990       1000       1010       1020 
IKNTQNLLHM PYGCGEQNMV LFAPNIYVLK YLDKTQQLTQ KIKTKALGFL RAGYQRELNY 

      1030       1040       1050       1060       1070       1080 
KHKDGSYSAF GDQNGEREGN TWLTAFVLKS FAQARAFIFI DESHITHAFT WLSQQQKDNG 

      1090       1100       1110       1120       1130       1140 
CFRSSGSLFH NDIKHPVVSK ALSCLESSWK TIEQGRNANF VYTKALMAYA FALAGNQDKR 

      1150       1160       1170       1180       1190       1200 
NEILKSLDEE AIKEDNSIHW ERPQKPRKSE HNLYKPQASS VEVEMNAYVV LARLTAQPAP 

      1210       1220       1230       1240       1250       1260 
SPEDLTLSRS TIMWLTKQQN SNGGFSSTQD TVVALDALSK YGAVTFSRRQ KTSLVTIQST 

      1270       1280       1290       1300       1310       1320 
GSFSQKFQVE NSNCLLLQQV PLPDIPGDYT ISVSGEGCVY AQTTLRYNMH LEKQQSAFAL 

      1330       1340       1350       1360       1370       1380 
RVQTVPLTCN NPKGHNSFQI SLEISYTGSR PASNMVIADV KMLSGFIPLK PTVKKLERLE 

      1390       1400       1410       1420       1430       1440 
HISRTEVSNN NVLLYLDQVT NQTLAFSFII QQDISVRNLQ PAIVKVYDYY ETDEVAYAEY 

      1450 
SSPCSSDKQN V 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M65238 mRNA. Translation: AAA73041.1.
AC153582 Genomic DNA. No translation available.
AC153838 Genomic DNA. No translation available.
AC156279 Genomic DNA. No translation available.
PIRB41185.
RefSeqNP_032672.2. NM_008646.3.
UniGeneMm.479223.

3D structure databases

ProteinModelPortalP28666.
SMRP28666. Positions 31-621, 671-1446.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP28666. 1 interaction.
MINTMINT-1849506.
STRING10090.ENSMUSP00000080469.

Protein family/group databases

MEROPSI39.005.

Proteomic databases

PaxDbP28666.
PRIDEP28666.

Protocols and materials databases

DNASU17837.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000081777; ENSMUSP00000080469; ENSMUSG00000030131.
GeneID17837.
KEGGmmu:17837.
UCSCuc009dos.1. mouse.

Organism-specific databases

CTD17837.
MGIMGI:99836. Mug2.

Phylogenomic databases

eggNOGCOG2373.
GeneTreeENSGT00740000115177.
HOGENOMHOG000220939.
HOVERGENHBG000039.
InParanoidP28666.
OMARTNMVII.
OrthoDBEOG7PGDPR.
TreeFamTF313285.

Gene expression databases

BgeeP28666.
CleanExMM_MUG2.
GenevestigatorP28666.

Family and domain databases

Gene3D1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR010916. TonB_box_CS.
[Graphical view]
PfamPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292555.
PROP28666.
SOURCESearch...

Entry information

Entry nameMUG2_MOUSE
AccessionPrimary (citable) accession number: P28666
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 28, 2011
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot