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Protein

Murinoglobulin-2

Gene

Mug2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

A proteinase activates the inhibitor by specific proteolysis in the bait region, which, by an unknown mechanism leads to reaction at the cysteinyl-glutamyl internal thiol ester site and to a conformational change, whereby the proteinase is trapped and/or covalently bound to the inhibitor. While in the tetrameric proteinase inhibitors steric inhibition is sufficiently strong, monomeric forms need a covalent linkage between the activated glutamyl residue of the original thiol ester and a terminal amino group of a lysine or another nucleophilic group on the proteinase, for inhibition to be effective.

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI39.005.

Names & Taxonomyi

Protein namesi
Recommended name:
Murinoglobulin-2
Short name:
MuG2
Gene namesi
Name:Mug2
Synonyms:Mug-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:99836. Mug2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727By similarityAdd
BLAST
Chaini28 – 14511424Murinoglobulin-2PRO_0000000060Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 86By similarity
Glycosylationi55 – 551N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi251 ↔ 276By similarity
Disulfide bondi269 ↔ 288By similarity
Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi313 – 3131N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi461 ↔ 555By similarity
Glycosylationi500 – 5001N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi587 ↔ 773By similarity
Disulfide bondi634 ↔ 680By similarity
Glycosylationi749 – 7491N-linked (GlcNAc...)Sequence Analysis
Glycosylationi776 – 7761N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi849 ↔ 885By similarity
Glycosylationi871 – 8711N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi923 ↔ 1274By similarity
Cross-linki974 ↔ 977Isoglutamyl cysteine thioester (Cys-Gln)By similarity
Disulfide bondi1081 ↔ 1104By similarity
Disulfide bondi1298 ↔ 1444By similarity
Glycosylationi1401 – 14011N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, Thioester bond

Proteomic databases

MaxQBiP28666.
PaxDbiP28666.
PRIDEiP28666.

Expressioni

Tissue specificityi

Plasma.

Gene expression databases

BgeeiP28666.
CleanExiMM_MUG2.
GenevestigatoriP28666.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP28666. 1 interaction.
MINTiMINT-1849506.
STRINGi10090.ENSMUSP00000080469.

Structurei

3D structure databases

ProteinModelPortaliP28666.
SMRiP28666. Positions 31-621, 733-1445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni677 – 73458Bait regionAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Bait region, Signal

Phylogenomic databases

eggNOGiCOG2373.
GeneTreeiENSGT00760000118982.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiP28666.
OMAiKLDNNGC.
OrthoDBiEOG7PGDPR.
TreeFamiTF313285.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR010916. TonB_box_CS.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWKSRRAQLC LFSVLLAFLP SASSLNGDSK YMVLVPSQLY TETPEKICLH
60 70 80 90 100
LYHLNETVTV TASLVSQTGR RNLFDELVVD KDLFQCVSFI IPTLNSPDEE
110 120 130 140 150
EFLYVDIKGP THEFSKRNAV LVKNKESVVF VQTDKPVYKP GQSVKFRVVS
160 170 180 190 200
MDKTLRPLNE LLPLAYIEDP KKNRIMQWRD IKTENGLKQM SFSLAAEPIQ
210 220 230 240 250
GPYKIVVHKQ SGVKEEHSFT VMEFVLPRFN VDLKVPNAIS VNDEVLQVTV
260 270 280 290 300
CGKYTYGKPV PGQVKISICH ETEAGCKEVN SKLDNNGCST QEVNITELQS
310 320 330 340 350
KKRNYEVQLF HVNATVTEEG TGLEFNGYGT TKIERITNKL IFLKADSHFR
360 370 380 390 400
HGIPFFVKVR LVDIKGDPIP NERVFIKAQV LGYTSATTTD QHGLAKFSID
410 420 430 440 450
TAGFSGSSLH IKVNHKGKDS CYFFYCMEER YASAEHVAYA VYSLSKSYIY
460 470 480 490 500
LVKETSSILP CNQIHTVQAH FILKGDLGVL KELVFYYLVM AQGSIIQTGN
510 520 530 540 550
HTHQVEPGEA PVKGNFDLEI PVEFSMAPMA KMLIYTILPD GEVIADSVNF
560 570 580 590 600
EIEKCLRNKV DLSFSSSQSL PASQTRLQVT ASPQSLCGLR AVDQSVLLLK
610 620 630 640 650
PEDELSPSWI YNLPGMQHNK FIPSSSLSED REDCILYSSW VAEKHTDWVP
660 670 680 690 700
HGREKDVYRY VEDMDLKAFT NLKIKLPKIC FDSAPMSGPR GKFDLAFSSE
710 720 730 740 750
VSGTLQKGSS KRPQPEEPPR EDPPPKDPLA ETIRKYFPET WVWDIVTVNS
760 770 780 790 800
TGVAEVEMTV PDTITEWKAG ALCLSNDTGL GLSSVVPLQA FQPFFVEVSL
810 820 830 840 850
PYSVVRGEAF MLKATVMNYL PTSMRMSVQL EASPDFTAVP VGDDHDSYCL
860 870 880 890 900
SANGRHTSSW LVTPKSLGNV NFSVSVEAQQ SSEPCGSEVA TVPETGRKDT
910 920 930 940 950
VVKVLIVEPE GIKQEHTFNS LFCASDAEIS EKMSLVLPPT VVKDSARAHF
960 970 980 990 1000
SVMGDILSSA IKNTQNLLHM PYGCGEQNMV LFAPNIYVLK YLDKTQQLTQ
1010 1020 1030 1040 1050
KIKTKALGFL RAGYQRELNY KHKDGSYSAF GDQNGEREGN TWLTAFVLKS
1060 1070 1080 1090 1100
FAQARAFIFI DESHITHAFT WLSQQQKDNG CFRSSGSLFH NDIKHPVVSK
1110 1120 1130 1140 1150
ALSCLESSWK TIEQGRNANF VYTKALMAYA FALAGNQDKR NEILKSLDEE
1160 1170 1180 1190 1200
AIKEDNSIHW ERPQKPRKSE HNLYKPQASS VEVEMNAYVV LARLTAQPAP
1210 1220 1230 1240 1250
SPEDLTLSRS TIMWLTKQQN SNGGFSSTQD TVVALDALSK YGAVTFSRRQ
1260 1270 1280 1290 1300
KTSLVTIQST GSFSQKFQVE NSNCLLLQQV PLPDIPGDYT ISVSGEGCVY
1310 1320 1330 1340 1350
AQTTLRYNMH LEKQQSAFAL RVQTVPLTCN NPKGHNSFQI SLEISYTGSR
1360 1370 1380 1390 1400
PASNMVIADV KMLSGFIPLK PTVKKLERLE HISRTEVSNN NVLLYLDQVT
1410 1420 1430 1440 1450
NQTLAFSFII QQDISVRNLQ PAIVKVYDYY ETDEVAYAEY SSPCSSDKQN

V
Length:1,451
Mass (Da):162,382
Last modified:June 28, 2011 - v2
Checksum:i8DB472A6C033C35D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti386 – 3861A → T in AAA73041 (PubMed:1840592).Curated
Sequence conflicti894 – 8941E → A in AAA73041 (PubMed:1840592).Curated
Sequence conflicti1181 – 11811V → I in AAA73041 (PubMed:1840592).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65238 mRNA. Translation: AAA73041.1.
AC153582 Genomic DNA. No translation available.
AC153838 Genomic DNA. No translation available.
AC156279 Genomic DNA. No translation available.
CCDSiCCDS39619.1.
PIRiB41185.
RefSeqiNP_032672.2. NM_008646.3.
UniGeneiMm.479223.

Genome annotation databases

EnsembliENSMUST00000081777; ENSMUSP00000080469; ENSMUSG00000030131.
GeneIDi17837.
KEGGimmu:17837.
UCSCiuc009dos.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65238 mRNA. Translation: AAA73041.1.
AC153582 Genomic DNA. No translation available.
AC153838 Genomic DNA. No translation available.
AC156279 Genomic DNA. No translation available.
CCDSiCCDS39619.1.
PIRiB41185.
RefSeqiNP_032672.2. NM_008646.3.
UniGeneiMm.479223.

3D structure databases

ProteinModelPortaliP28666.
SMRiP28666. Positions 31-621, 733-1445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28666. 1 interaction.
MINTiMINT-1849506.
STRINGi10090.ENSMUSP00000080469.

Protein family/group databases

MEROPSiI39.005.

Proteomic databases

MaxQBiP28666.
PaxDbiP28666.
PRIDEiP28666.

Protocols and materials databases

DNASUi17837.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000081777; ENSMUSP00000080469; ENSMUSG00000030131.
GeneIDi17837.
KEGGimmu:17837.
UCSCiuc009dos.1. mouse.

Organism-specific databases

CTDi17837.
MGIiMGI:99836. Mug2.

Phylogenomic databases

eggNOGiCOG2373.
GeneTreeiENSGT00760000118982.
HOGENOMiHOG000220939.
HOVERGENiHBG000039.
InParanoidiP28666.
OMAiKLDNNGC.
OrthoDBiEOG7PGDPR.
TreeFamiTF313285.

Miscellaneous databases

NextBioi292555.
PROiP28666.
SOURCEiSearch...

Gene expression databases

BgeeiP28666.
CleanExiMM_MUG2.
GenevestigatoriP28666.

Family and domain databases

Gene3Di1.50.10.20. 1 hit.
2.60.40.690. 1 hit.
InterProiIPR009048. A-macroglobulin_rcpt-bd.
IPR011626. A2M_comp.
IPR002890. A2M_N.
IPR011625. A2M_N_2.
IPR014756. Ig_E-set.
IPR001599. Macroglobln_a2.
IPR019742. MacrogloblnA2_CS.
IPR019565. MacrogloblnA2_thiol-ester-bond.
IPR008930. Terpenoid_cyclase/PrenylTrfase.
IPR010916. TonB_box_CS.
[Graphical view]
PfamiPF00207. A2M. 1 hit.
PF07678. A2M_comp. 1 hit.
PF01835. A2M_N. 1 hit.
PF07703. A2M_N_2. 1 hit.
PF07677. A2M_recep. 1 hit.
PF10569. Thiol-ester_cl. 1 hit.
[Graphical view]
SUPFAMiSSF48239. SSF48239. 1 hit.
SSF49410. SSF49410. 1 hit.
SSF81296. SSF81296. 1 hit.
PROSITEiPS00477. ALPHA_2_MACROGLOBULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of the murinoglobulins."
    Overbergh L., Torrekens S., van Leuven F., van den Berghe H.
    J. Biol. Chem. 266:16903-16910(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiMUG2_MOUSE
AccessioniPrimary (citable) accession number: P28666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: June 28, 2011
Last modified: January 7, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.