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Protein

Septin-4

Gene

Sept4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185GTPBy similarity1
Binding sitei211GTP; via amide nitrogenBy similarity1
Binding sitei348GTP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei363GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 158GTPBy similarity8
Nucleotide bindingi290 – 298GTPBy similarity9

GO - Molecular functioni

GO - Biological processi

  • brain development Source: MGI
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of protein ubiquitination Source: MGI
  • spermatid development Source: MGI
  • sperm capacitation Source: MGI
  • sperm mitochondrion organization Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Septin-4
Alternative name(s):
Brain protein H5
Peanut-like protein 2
Gene namesi
Name:Sept4
Synonyms:Pnutl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1270156. Sept4.

Subcellular locationi

Isoform 4 :
  • Mitochondrion

  • Note: In retinoic acid-treated P19 cells, isoform 4 is found first in the mitochondria and at later times, as neuronal differentiation proceeds, in the cytosol.

GO - Cellular componenti

  • cell projection Source: MGI
  • cytoskeleton Source: UniProtKB-SubCell
  • mitochondrion Source: MGI
  • motile cilium Source: MGI
  • myelin sheath Source: UniProtKB
  • nucleoplasm Source: MGI
  • sperm annulus Source: MGI
  • sperm flagellum Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001735201 – 478Septin-4Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei118PhosphoserineCombined sources1
Modified residuei325PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei434PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28661.
PaxDbiP28661.
PeptideAtlasiP28661.
PRIDEiP28661.

PTM databases

iPTMnetiP28661.
PhosphoSitePlusiP28661.

Expressioni

Tissue specificityi

Expressed in the brain. Abundant in areas of high cell density, particularly in the stria terminalis. Isoform 4 is predominantly expressed in embryonic mouse brain and dorsal root ganglion neurons.1 Publication

Developmental stagei

Widely expressed in embryos at 7 dpc. Isoform 4 predominantly expressed in the brain at 14 dpc and on postnatal day 1.1 Publication

Gene expression databases

BgeeiENSMUSG00000020486.
CleanExiMM_SEPT4.
ExpressionAtlasiP28661. baseline and differential.
GenevisibleiP28661. MM.

Interactioni

Subunit structurei

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with SEPT8 (By similarity). Isoform 4 interacts with DPYSL5.By similarity1 Publication

Protein-protein interaction databases

BioGridi202286. 5 interactors.
IntActiP28661. 3 interactors.
MINTiMINT-4134142.
STRINGi10090.ENSMUSP00000018544.

Structurei

3D structure databases

ProteinModelPortaliP28661.
SMRiP28661.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 414Septin-type GAdd BLAST274

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili447 – 478Sequence analysisAdd BLAST32

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2655. Eukaryota.
COG5019. LUCA.
GeneTreeiENSGT00860000133688.
HOVERGENiHBG065093.
InParanoidiP28661.
KOiK16943.
OMAiPPEVDRK.
OrthoDBiEOG091G07TS.
PhylomeDBiP28661.
TreeFamiTF101079.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 2 hits.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
IPR030643. Septin4.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PTHR18884:SF71. PTHR18884:SF71. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28661-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHSLGWQGN SVPEDGTEAG IKHFLEDSSD DAELSKFVKD FPGSEPYHSA
60 70 80 90 100
ESKTRVARPQ ILEPRPQSPD LCDDDVEFRG SLWPQPSDSQ QYFSAPAPLS
110 120 130 140 150
PSSRPRSPWG KLDPYDSSED DKEYVGFATL PNQVHRKSVK KGFDFTLMVA
160 170 180 190 200
GESGLGKSTL VNSLFLTDLY RDRKLLGAEE RIMQTVEITK HAVDIEEKGV
210 220 230 240 250
RLRLTIVDTP GFGDAVNNTE CWKPVAEYID QQFEQYFRDE SGLNRKNIQD
260 270 280 290 300
NRVHCCLYFI SPFGHGLRPL DVEFMKALHQ RVNIVPILAK ADTLTPPEVD
310 320 330 340 350
RKKCKIREEI EHFGIKIYQF PDCDSDEDED FKLQDQALKE SIPFAVIGSN
360 370 380 390 400
TVVEARGRRV RGRLYPWGIV EVENPGHCDF VKLRTMLVRT HMQDLKDVTR
410 420 430 440 450
ETHYENYRAQ CIQSMTRLVV KERNRNKLTR ESGTDFPIPA VPPGTDPETE
460 470
KLIREKDEEL RRMQEMLHKI QRQMKETH
Length:478
Mass (Da):54,936
Last modified:December 1, 1992 - v1
Checksum:i36655DE54A6C4908
GO
Isoform 2 (identifier: P28661-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MDHSLGWQGNSVPEDGTEAG → M

Show »
Length:459
Mass (Da):52,998
Checksum:iB47573B04A6CCE67
GO
Isoform 3 (identifier: P28661-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     426-431: NKLTRE → KDRSRN
     432-478: Missing.

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):49,425
Checksum:i9768B713C998B739
GO
Isoform 4 (identifier: P28661-4) [UniParc]FASTAAdd to basket
Also known as: Mitochindrial septin, M-septin

The sequence of this isoform differs from the canonical sequence as follows:
     21-119: Missing.

Show »
Length:379
Mass (Da):43,789
Checksum:i4B31F40666EFD942
GO
Isoform 5 (identifier: P28661-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.
     119-119: E → M
     426-431: NKLTRE → KDRSRN
     432-478: Missing.

Note: No experimental confirmation available.
Show »
Length:313
Mass (Da):36,340
Checksum:iD69F63E697F5DB5D
GO
Isoform 6 (identifier: P28661-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MDHSLGWQGNSVPEDGTEAG → M
     221-223: CWK → WYV
     224-478: Missing.

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):22,983
Checksum:i1B5EC688E0025132
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti394D → N in BAE21934 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383071 – 118Missing in isoform 5. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_0383081 – 20MDHSL…GTEAG → M in isoform 2 and isoform 6. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_03830921 – 119Missing in isoform 4. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_038310119E → M in isoform 5. 1 Publication1
Alternative sequenceiVSP_038311221 – 223CWK → WYV in isoform 6. 1 Publication3
Alternative sequenceiVSP_038312224 – 478Missing in isoform 6. 1 PublicationAdd BLAST255
Alternative sequenceiVSP_038313426 – 431NKLTRE → KDRSRN in isoform 3 and isoform 5. 2 Publications6
Alternative sequenceiVSP_038314432 – 478Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61452 mRNA. Translation: CAA43692.1.
AB078010 mRNA. Translation: BAC55241.1.
AK133933 mRNA. Translation: BAE21934.1.
AK135143 mRNA. Translation: BAE22437.1.
AK137299 mRNA. Translation: BAE23298.1.
AL596086 Genomic DNA. Translation: CAI35128.2.
AL596086 Genomic DNA. Translation: CAI35129.1.
AL596086 Genomic DNA. Translation: CAI35132.1.
AL596086 Genomic DNA. Translation: CAI35133.1.
AL596086 Genomic DNA. Translation: CAI35134.1.
CU406988 Genomic DNA. Translation: CAQ52271.1.
CU406988 Genomic DNA. Translation: CAQ52272.1.
CU406988 Genomic DNA. Translation: CAQ52274.1.
CU406988 Genomic DNA. Translation: CAQ52275.1.
CU406988 Genomic DNA. Translation: CAQ52276.1.
CH466556 Genomic DNA. Translation: EDL15822.1.
BC055101 mRNA. Translation: AAH55101.1.
CCDSiCCDS25213.1. [P28661-1]
CCDS70275.1. [P28661-5]
CCDS70276.1. [P28661-3]
CCDS70277.1. [P28661-4]
PIRiS16867.
RefSeqiNP_001271321.1. NM_001284392.1. [P28661-4]
NP_001271323.1. NM_001284394.1. [P28661-3]
NP_001271327.1. NM_001284398.1. [P28661-5]
NP_035259.1. NM_011129.2. [P28661-1]
XP_006532561.1. XM_006532498.3. [P28661-2]
UniGeneiMm.2214.

Genome annotation databases

EnsembliENSMUST00000018544; ENSMUSP00000018544; ENSMUSG00000020486. [P28661-1]
ENSMUST00000063156; ENSMUSP00000060127; ENSMUSG00000020486. [P28661-4]
ENSMUST00000107960; ENSMUSP00000103594; ENSMUSG00000020486. [P28661-3]
ENSMUST00000107962; ENSMUSP00000103596; ENSMUSG00000020486. [P28661-2]
ENSMUST00000122067; ENSMUSP00000112960; ENSMUSG00000020486. [P28661-5]
GeneIDi18952.
KEGGimmu:18952.
UCSCiuc007kts.2. mouse. [P28661-6]
uc007ktu.2. mouse. [P28661-5]
uc007ktw.2. mouse. [P28661-1]
uc007ktx.2. mouse. [P28661-3]
uc007kty.2. mouse. [P28661-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61452 mRNA. Translation: CAA43692.1.
AB078010 mRNA. Translation: BAC55241.1.
AK133933 mRNA. Translation: BAE21934.1.
AK135143 mRNA. Translation: BAE22437.1.
AK137299 mRNA. Translation: BAE23298.1.
AL596086 Genomic DNA. Translation: CAI35128.2.
AL596086 Genomic DNA. Translation: CAI35129.1.
AL596086 Genomic DNA. Translation: CAI35132.1.
AL596086 Genomic DNA. Translation: CAI35133.1.
AL596086 Genomic DNA. Translation: CAI35134.1.
CU406988 Genomic DNA. Translation: CAQ52271.1.
CU406988 Genomic DNA. Translation: CAQ52272.1.
CU406988 Genomic DNA. Translation: CAQ52274.1.
CU406988 Genomic DNA. Translation: CAQ52275.1.
CU406988 Genomic DNA. Translation: CAQ52276.1.
CH466556 Genomic DNA. Translation: EDL15822.1.
BC055101 mRNA. Translation: AAH55101.1.
CCDSiCCDS25213.1. [P28661-1]
CCDS70275.1. [P28661-5]
CCDS70276.1. [P28661-3]
CCDS70277.1. [P28661-4]
PIRiS16867.
RefSeqiNP_001271321.1. NM_001284392.1. [P28661-4]
NP_001271323.1. NM_001284394.1. [P28661-3]
NP_001271327.1. NM_001284398.1. [P28661-5]
NP_035259.1. NM_011129.2. [P28661-1]
XP_006532561.1. XM_006532498.3. [P28661-2]
UniGeneiMm.2214.

3D structure databases

ProteinModelPortaliP28661.
SMRiP28661.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202286. 5 interactors.
IntActiP28661. 3 interactors.
MINTiMINT-4134142.
STRINGi10090.ENSMUSP00000018544.

PTM databases

iPTMnetiP28661.
PhosphoSitePlusiP28661.

Proteomic databases

MaxQBiP28661.
PaxDbiP28661.
PeptideAtlasiP28661.
PRIDEiP28661.

Protocols and materials databases

DNASUi18952.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018544; ENSMUSP00000018544; ENSMUSG00000020486. [P28661-1]
ENSMUST00000063156; ENSMUSP00000060127; ENSMUSG00000020486. [P28661-4]
ENSMUST00000107960; ENSMUSP00000103594; ENSMUSG00000020486. [P28661-3]
ENSMUST00000107962; ENSMUSP00000103596; ENSMUSG00000020486. [P28661-2]
ENSMUST00000122067; ENSMUSP00000112960; ENSMUSG00000020486. [P28661-5]
GeneIDi18952.
KEGGimmu:18952.
UCSCiuc007kts.2. mouse. [P28661-6]
uc007ktu.2. mouse. [P28661-5]
uc007ktw.2. mouse. [P28661-1]
uc007ktx.2. mouse. [P28661-3]
uc007kty.2. mouse. [P28661-4]

Organism-specific databases

CTDi5414.
MGIiMGI:1270156. Sept4.

Phylogenomic databases

eggNOGiKOG2655. Eukaryota.
COG5019. LUCA.
GeneTreeiENSGT00860000133688.
HOVERGENiHBG065093.
InParanoidiP28661.
KOiK16943.
OMAiPPEVDRK.
OrthoDBiEOG091G07TS.
PhylomeDBiP28661.
TreeFamiTF101079.

Miscellaneous databases

PROiP28661.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020486.
CleanExiMM_SEPT4.
ExpressionAtlasiP28661. baseline and differential.
GenevisibleiP28661. MM.

Family and domain databases

CDDicd01850. CDC_Septin. 1 hit.
Gene3Di3.40.50.300. 2 hits.
InterProiIPR030379. G_SEPTIN_dom.
IPR027417. P-loop_NTPase.
IPR016491. Septin.
IPR030643. Septin4.
[Graphical view]
PANTHERiPTHR18884. PTHR18884. 1 hit.
PTHR18884:SF71. PTHR18884:SF71. 1 hit.
PfamiPF00735. Septin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS51719. G_SEPTIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSEPT4_MOUSE
AccessioniPrimary (citable) accession number: P28661
Secondary accession number(s): B2KGM6
, Q3UVH1, Q3UZC3, Q5ND10, Q5ND15, Q5ND16, Q7TPM7, Q80VX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: November 30, 2016
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.