Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Septin-4

Gene

Sept4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Filament-forming cytoskeletal GTPase. Forms a filamentous structure with SEPT12, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity). May play a role in cytokinesis (Potential). May play a role in platelet secretion (By similarity).By similarityCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185GTPBy similarity1
Binding sitei211GTP; via amide nitrogenBy similarity1
Binding sitei348GTP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei363GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi151 – 158GTPBy similarity8
Nucleotide bindingi290 – 298GTPBy similarity9

GO - Molecular functioni

GO - Biological processi

  • brain development Source: MGI
  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of intrinsic apoptotic signaling pathway Source: MGI
  • positive regulation of protein ubiquitination Source: MGI
  • protein homooligomerization Source: MGI
  • spermatid development Source: MGI
  • sperm capacitation Source: MGI
  • sperm mitochondrion organization Source: MGI

Keywordsi

Biological processCell cycle, Cell division, Differentiation, Spermatogenesis
LigandGTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Septin-4
Alternative name(s):
Brain protein H5
Peanut-like protein 2
Gene namesi
Name:Sept4
Synonyms:Pnutl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1270156 Sept4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cilium, Cytoplasm, Cytoskeleton, Flagellum, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001735201 – 478Septin-4Add BLAST478

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1
Modified residuei29PhosphoserineCombined sources1
Modified residuei68PhosphoserineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei118PhosphoserineCombined sources1
Modified residuei325PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei434PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP28661
PaxDbiP28661
PeptideAtlasiP28661
PRIDEiP28661

PTM databases

iPTMnetiP28661
PhosphoSitePlusiP28661

Expressioni

Tissue specificityi

Expressed in the brain. Abundant in areas of high cell density, particularly in the stria terminalis. Isoform 4 is predominantly expressed in embryonic mouse brain and dorsal root ganglion neurons.1 Publication

Developmental stagei

Widely expressed in embryos at 7 dpc. Isoform 4 predominantly expressed in the brain at 14 dpc and on postnatal day 1.1 Publication

Gene expression databases

BgeeiENSMUSG00000020486
CleanExiMM_SEPT4
ExpressionAtlasiP28661 baseline and differential
GenevisibleiP28661 MM

Interactioni

Subunit structurei

Septins polymerize into heterooligomeric protein complexes that form filaments, and can associate with cellular membranes, actin filaments and microtubules. GTPase activity is required for filament formation (By similarity). Interacts with SEPT8 (By similarity). Component of a septin core octomeric complex consisting of SEPT12, SEPT7, SEPT6 and SEPT2 or SEPT4 in the order 12-7-6-2-2-6-7-12 or 12-7-6-4-4-6-7-12 and located in the sperm annulus (By similarity). Isoform 4 interacts with DPYSL5.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202286, 5 interactors
IntActiP28661, 3 interactors
MINTiP28661
STRINGi10090.ENSMUSP00000018544

Structurei

3D structure databases

ProteinModelPortaliP28661
SMRiP28661
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini141 – 414Septin-type GAdd BLAST274

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili447 – 478Sequence analysisAdd BLAST32

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2655 Eukaryota
COG5019 LUCA
GeneTreeiENSGT00910000144020
HOVERGENiHBG065093
InParanoidiP28661
KOiK16943
OMAiFSGNESC
OrthoDBiEOG091G07TS
PhylomeDBiP28661
TreeFamiTF101079

Family and domain databases

CDDicd01850 CDC_Septin, 1 hit
InterProiView protein in InterPro
IPR030379 G_SEPTIN_dom
IPR027417 P-loop_NTPase
IPR016491 Septin
IPR030643 Septin4
PANTHERiPTHR18884:SF71 PTHR18884:SF71, 1 hit
PfamiView protein in Pfam
PF00735 Septin, 1 hit
PIRSFiPIRSF006698 Septin, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51719 G_SEPTIN, 1 hit

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28661-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDHSLGWQGN SVPEDGTEAG IKHFLEDSSD DAELSKFVKD FPGSEPYHSA
60 70 80 90 100
ESKTRVARPQ ILEPRPQSPD LCDDDVEFRG SLWPQPSDSQ QYFSAPAPLS
110 120 130 140 150
PSSRPRSPWG KLDPYDSSED DKEYVGFATL PNQVHRKSVK KGFDFTLMVA
160 170 180 190 200
GESGLGKSTL VNSLFLTDLY RDRKLLGAEE RIMQTVEITK HAVDIEEKGV
210 220 230 240 250
RLRLTIVDTP GFGDAVNNTE CWKPVAEYID QQFEQYFRDE SGLNRKNIQD
260 270 280 290 300
NRVHCCLYFI SPFGHGLRPL DVEFMKALHQ RVNIVPILAK ADTLTPPEVD
310 320 330 340 350
RKKCKIREEI EHFGIKIYQF PDCDSDEDED FKLQDQALKE SIPFAVIGSN
360 370 380 390 400
TVVEARGRRV RGRLYPWGIV EVENPGHCDF VKLRTMLVRT HMQDLKDVTR
410 420 430 440 450
ETHYENYRAQ CIQSMTRLVV KERNRNKLTR ESGTDFPIPA VPPGTDPETE
460 470
KLIREKDEEL RRMQEMLHKI QRQMKETH
Length:478
Mass (Da):54,936
Last modified:December 1, 1992 - v1
Checksum:i36655DE54A6C4908
GO
Isoform 2 (identifier: P28661-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MDHSLGWQGNSVPEDGTEAG → M

Show »
Length:459
Mass (Da):52,998
Checksum:iB47573B04A6CCE67
GO
Isoform 3 (identifier: P28661-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     426-431: NKLTRE → KDRSRN
     432-478: Missing.

Note: No experimental confirmation available.
Show »
Length:431
Mass (Da):49,425
Checksum:i9768B713C998B739
GO
Isoform 4 (identifier: P28661-4) [UniParc]FASTAAdd to basket
Also known as: Mitochondrial septin, M-septin

The sequence of this isoform differs from the canonical sequence as follows:
     21-119: Missing.

Show »
Length:379
Mass (Da):43,789
Checksum:i4B31F40666EFD942
GO
Isoform 5 (identifier: P28661-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-118: Missing.
     119-119: E → M
     426-431: NKLTRE → KDRSRN
     432-478: Missing.

Note: No experimental confirmation available.
Show »
Length:313
Mass (Da):36,340
Checksum:iD69F63E697F5DB5D
GO
Isoform 6 (identifier: P28661-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: MDHSLGWQGNSVPEDGTEAG → M
     221-223: CWK → WYV
     224-478: Missing.

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):22,983
Checksum:i1B5EC688E0025132
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti394D → N in BAE21934 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0383071 – 118Missing in isoform 5. 1 PublicationAdd BLAST118
Alternative sequenceiVSP_0383081 – 20MDHSL…GTEAG → M in isoform 2 and isoform 6. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_03830921 – 119Missing in isoform 4. 1 PublicationAdd BLAST99
Alternative sequenceiVSP_038310119E → M in isoform 5. 1 Publication1
Alternative sequenceiVSP_038311221 – 223CWK → WYV in isoform 6. 1 Publication3
Alternative sequenceiVSP_038312224 – 478Missing in isoform 6. 1 PublicationAdd BLAST255
Alternative sequenceiVSP_038313426 – 431NKLTRE → KDRSRN in isoform 3 and isoform 5. 2 Publications6
Alternative sequenceiVSP_038314432 – 478Missing in isoform 3 and isoform 5. 2 PublicationsAdd BLAST47

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61452 mRNA Translation: CAA43692.1
AB078010 mRNA Translation: BAC55241.1
AK133933 mRNA Translation: BAE21934.1
AK135143 mRNA Translation: BAE22437.1
AK137299 mRNA Translation: BAE23298.1
AL596086 Genomic DNA Translation: CAI35128.2
AL596086 Genomic DNA Translation: CAI35129.1
AL596086 Genomic DNA Translation: CAI35132.1
AL596086 Genomic DNA Translation: CAI35133.1
AL596086 Genomic DNA Translation: CAI35134.1
CU406988 Genomic DNA Translation: CAQ52271.1
CU406988 Genomic DNA Translation: CAQ52272.1
CU406988 Genomic DNA Translation: CAQ52274.1
CU406988 Genomic DNA Translation: CAQ52275.1
CU406988 Genomic DNA Translation: CAQ52276.1
CH466556 Genomic DNA Translation: EDL15822.1
BC055101 mRNA Translation: AAH55101.1
CCDSiCCDS25213.1 [P28661-1]
CCDS70275.1 [P28661-5]
CCDS70276.1 [P28661-3]
CCDS70277.1 [P28661-4]
PIRiS16867
RefSeqiNP_001271321.1, NM_001284392.1 [P28661-4]
NP_001271323.1, NM_001284394.1 [P28661-3]
NP_001271327.1, NM_001284398.1 [P28661-5]
NP_035259.1, NM_011129.2 [P28661-1]
XP_006532561.1, XM_006532498.3 [P28661-2]
UniGeneiMm.2214

Genome annotation databases

EnsembliENSMUST00000018544; ENSMUSP00000018544; ENSMUSG00000020486 [P28661-1]
ENSMUST00000063156; ENSMUSP00000060127; ENSMUSG00000020486 [P28661-4]
ENSMUST00000107960; ENSMUSP00000103594; ENSMUSG00000020486 [P28661-3]
ENSMUST00000107962; ENSMUSP00000103596; ENSMUSG00000020486 [P28661-2]
ENSMUST00000122067; ENSMUSP00000112960; ENSMUSG00000020486 [P28661-5]
GeneIDi18952
KEGGimmu:18952
UCSCiuc007kts.2 mouse [P28661-6]
uc007ktu.2 mouse [P28661-5]
uc007ktw.2 mouse [P28661-1]
uc007ktx.2 mouse [P28661-3]
uc007kty.2 mouse [P28661-4]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSEPT4_MOUSE
AccessioniPrimary (citable) accession number: P28661
Secondary accession number(s): B2KGM6
, Q3UVH1, Q3UZC3, Q5ND10, Q5ND15, Q5ND16, Q7TPM7, Q80VX1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: April 25, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health