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Protein

Nck-associated protein 1

Gene

Nckap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.1 Publication

GO - Molecular functioni

GO - Biological processi

  • apical protein localization Source: MGI
  • basal protein localization Source: MGI
  • cell migration involved in gastrulation Source: MGI
  • embryonic body morphogenesis Source: MGI
  • embryonic foregut morphogenesis Source: MGI
  • embryonic heart tube development Source: MGI
  • endoderm development Source: MGI
  • establishment or maintenance of actin cytoskeleton polarity Source: MGI
  • in utero embryonic development Source: MGI
  • lamellipodium assembly Source: MGI
  • mesodermal cell migration Source: MGI
  • neural tube closure Source: MGI
  • notochord development Source: MGI
  • notochord morphogenesis Source: MGI
  • paraxial mesoderm development Source: MGI
  • paraxial mesoderm morphogenesis Source: MGI
  • positive regulation of Arp2/3 complex-mediated actin nucleation Source: MGI
  • positive regulation of lamellipodium assembly Source: MGI
  • protein stabilization Source: MGI
  • Rac protein signal transduction Source: MGI
  • regulation of protein localization Source: MGI
  • somitogenesis Source: MGI
  • zygotic determination of anterior/posterior axis, embryo Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Names & Taxonomyi

Protein namesi
Recommended name:
Nck-associated protein 1
Short name:
NAP 1
Alternative name(s):
Brain protein H19
MH19
Membrane-associated protein HEM-2
p125Nap1
Gene namesi
Name:Nckap1
Synonyms:Hem2, Kiaa0587, Nap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1355333. Nckap1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei995 – 101521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 11281127Nck-associated protein 1PRO_0000216173Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP28660.
MaxQBiP28660.
PaxDbiP28660.
PeptideAtlasiP28660.
PRIDEiP28660.

PTM databases

iPTMnetiP28660.
PhosphoSiteiP28660.

Expressioni

Tissue specificityi

High expression in cerebral cortex, not in cerebellar cortex.

Gene expression databases

BgeeiP28660.
CleanExiMM_NCKAP1.
ExpressionAtlasiP28660. baseline and differential.
GenevisibleiP28660. MM.

Interactioni

Subunit structurei

Associates preferentially with the first SH3 domain of NCK (By similarity). Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Interacts with NYAP1, NYAP2 and MYO16.By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Myo16Q5DU142EBI-771576,EBI-7448308

GO - Molecular functioni

Protein-protein interaction databases

BioGridi206144. 5 interactions.
IntActiP28660. 10 interactions.
MINTiMINT-1861711.
STRINGi10090.ENSMUSP00000028386.

Structurei

3D structure databases

ProteinModelPortaliP28660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the HEM-1/HEM-2 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
GeneTreeiENSGT00390000016619.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP28660.
KOiK05750.
OrthoDBiEOG7TJ3H0.
TreeFamiTF313683.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P28660-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSRSVLQPSQ QKLAEKLTIL NDRGVGMLTR LYNIKKACGD PKAKPSYLID
60 70 80 90 100
KNLESAVKFI VRKFPAVETR NNNQQLAQLQ KEKSEILKNL ALYYFTFVDV
110 120 130 140 150
MEFKDHVCDL LNTIDVCQVF FDITVNFDLT KNYLDLTVTY TTLMILLSRI
160 170 180 190 200
EERKAIIGLY NYAHEMTHGA SDREYPRLGQ MIVDYENPLK KMMEEFVPHS
210 220 230 240 250
KSLSDALISL QMVYPRRNLS ADQWRNAQLL SLISAPSTML NPAQSDTMPC
260 270 280 290 300
EYLSLDAMEK WIIFGFILCH GMLNTEATAL NLWKLALQSS SCLSLFRDEV
310 320 330 340 350
FHIHKAAEDL FVNIRGYNKR INDIRECKEA AVSHAGSMHR ERRKFLRSAL
360 370 380 390 400
KELATVLSDQ PGLLGPKALF VFMALSFARD EIIWLLRHAD NMPKKSADDF
410 420 430 440 450
IDKHIAELIF YMEELRAHVR KYGPVMQRYY VQYLSGFDAV VLNELVQNLS
460 470 480 490 500
VCPEDESIIM SSFVNTMTSL SVKQVEDGEV FDFRGMRLDW FRLQAYTSVS
510 520 530 540 550
KASLSLADHR ELGKMMNTII FHTKMVDSLV EMLVETSDLS IFCFYSRAFE
560 570 580 590 600
KMFQQCLELP SQSRYSIAFP LLCTHFMSCT HELCPEERHH IGDRSLSLCN
610 620 630 640 650
MFLDEMAKQA RNLITDICTE QCTLSDQLLP KHCAKTISQA VNKKSKKQTG
660 670 680 690 700
KKGEPEREKP GVESMRKNRL VVTNLDKLHT ALSELCFSIN YVPNMAVWEH
710 720 730 740 750
TFTPREYLTS HLEIRFTKSI VGMTMYNQAT QEIAKPSELL TSVRAYMTVL
760 770 780 790 800
QSIENYVQID ITRVFNNVLL QQTQHLDSHG EPTITSLYTN WYLETLLRQV
810 820 830 840 850
SNGHIAYFPA MKAFVNLPTE NELTFNAEEY SDISEMRSLS ELLGPYGMKF
860 870 880 890 900
LSESLMWHIS SQVAELKKLV VENVDVLTQM RTSFDKPDQM AALFKRLSSV
910 920 930 940 950
DSVLKRMTII GVILSFRSLA QEALRDVLSY HIPFLVSSIE DFKDHIPRET
960 970 980 990 1000
DMKVAMNVYE LSSAAGLPCE IDPALVVALS SQKSENISPE EEYKIACLLM
1010 1020 1030 1040 1050
VFVAVSLPTL ASNVMSQYSP AIEGHCNNIH CLAKAINQIA AALFTIHKGS
1060 1070 1080 1090 1100
IEDRLKEFLA LASSSLLKIG QETDKTTTRN RESVYLLLDM IVQESPFLTM
1110 1120
DLLESCFPYV LLRNAYHAVY KQSVTSSA
Length:1,128
Mass (Da):128,784
Last modified:November 22, 2005 - v2
Checksum:i279246384B0F4B77
GO
Isoform 2 (identifier: P28660-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     242-263: Missing.
     1060-1128: Missing.

Note: No experimental confirmation available.
Show »
Length:1,037
Mass (Da):118,519
Checksum:iE66FE8450D5D1EEE
GO

Sequence cautioni

The sequence BAC65600.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti745 – 7451A → E in CAA43693 (PubMed:12106288).Curated
Sequence conflicti876 – 8761V → F in BAE25258 (PubMed:16141072).Curated
Sequence conflicti934 – 9352FL → SS in CAA43693 (PubMed:12106288).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei242 – 26322Missing in isoform 2. 1 PublicationVSP_016261Add
BLAST
Alternative sequencei1060 – 112869Missing in isoform 2. 1 PublicationVSP_016262Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ534525 mRNA. Translation: CAD58932.1.
AK122318 Transcribed RNA. Translation: BAC65600.1. Different initiation.
X61453 mRNA. Translation: CAA43693.1.
BC003962 mRNA. Translation: AAH03962.1.
AK143049 mRNA. Translation: BAE25258.1.
CCDSiCCDS16177.1. [P28660-1]
PIRiS16866.
RefSeqiNP_058661.1. NM_016965.3. [P28660-1]
UniGeneiMm.25203.

Genome annotation databases

EnsembliENSMUST00000028386; ENSMUSP00000028386; ENSMUSG00000027002. [P28660-1]
GeneIDi50884.
KEGGimmu:50884.
UCSCiuc008khm.2. mouse. [P28660-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ534525 mRNA. Translation: CAD58932.1.
AK122318 Transcribed RNA. Translation: BAC65600.1. Different initiation.
X61453 mRNA. Translation: CAA43693.1.
BC003962 mRNA. Translation: AAH03962.1.
AK143049 mRNA. Translation: BAE25258.1.
CCDSiCCDS16177.1. [P28660-1]
PIRiS16866.
RefSeqiNP_058661.1. NM_016965.3. [P28660-1]
UniGeneiMm.25203.

3D structure databases

ProteinModelPortaliP28660.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206144. 5 interactions.
IntActiP28660. 10 interactions.
MINTiMINT-1861711.
STRINGi10090.ENSMUSP00000028386.

PTM databases

iPTMnetiP28660.
PhosphoSiteiP28660.

Proteomic databases

EPDiP28660.
MaxQBiP28660.
PaxDbiP28660.
PeptideAtlasiP28660.
PRIDEiP28660.

Protocols and materials databases

DNASUi50884.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028386; ENSMUSP00000028386; ENSMUSG00000027002. [P28660-1]
GeneIDi50884.
KEGGimmu:50884.
UCSCiuc008khm.2. mouse. [P28660-1]

Organism-specific databases

CTDi10787.
MGIiMGI:1355333. Nckap1.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1917. Eukaryota.
ENOG410XPQI. LUCA.
GeneTreeiENSGT00390000016619.
HOGENOMiHOG000231880.
HOVERGENiHBG006344.
InParanoidiP28660.
KOiK05750.
OrthoDBiEOG7TJ3H0.
TreeFamiTF313683.

Enzyme and pathway databases

ReactomeiR-MMU-2029482. Regulation of actin dynamics for phagocytic cup formation.
R-MMU-4420097. VEGFA-VEGFR2 Pathway.
R-MMU-5663213. RHO GTPases Activate WASPs and WAVEs.

Miscellaneous databases

ChiTaRSiNckap1. mouse.
PROiP28660.
SOURCEiSearch...

Gene expression databases

BgeeiP28660.
CleanExiMM_NCKAP1.
ExpressionAtlasiP28660. baseline and differential.
GenevisibleiP28660. MM.

Family and domain databases

InterProiIPR019137. Nck-associated_protein-1.
[Graphical view]
PfamiPF09735. Nckap1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sra-1 and Nap1 link Rac to actin assembly driving lamellipodia formation."
    Steffen A., Rottner K., Ehinger J., Innocenti M., Scita G., Wehland J., Stradal T.E.B.
    EMBO J. 23:749-759(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION, COMPONENT OF WAVE2 COMPLEX.
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Prediction of the coding sequences of mouse homologues of KIAA gene: II. The complete nucleotide sequences of 400 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Aizawa H., Yuasa S., Nakajima D., Nagase T., Ohara O., Koga H.
    DNA Res. 10:35-48(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Brain.
  3. "A collection of cDNA clones with specific expression patterns in mouse brain."
    Kato K.
    Eur. J. Neurosci. 2:704-711(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 288-935 (ISOFORMS 1/2).
    Strain: BALB/cJ.
    Tissue: Brain.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 755-1128 (ISOFORM 1).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 876-1128 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Eye.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "NYAP: a phosphoprotein family that links PI3K to WAVE1 signalling in neurons."
    Yokoyama K., Tezuka T., Kotani M., Nakazawa T., Hoshina N., Shimoda Y., Kakuta S., Sudo K., Watanabe K., Iwakura Y., Yamamoto T.
    EMBO J. 30:4739-4754(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NYAP1; NYAP2 AND MYO16.

Entry informationi

Entry nameiNCKP1_MOUSE
AccessioniPrimary (citable) accession number: P28660
Secondary accession number(s): Q3UPY6
, Q80TX0, Q8CG49, Q99KY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.