Reviewed,
UniProtKB/Swiss-Prot P28652 (KCC2B_MOUSE)
Last modified
October 13, 2009.
Version 101.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Calcium/calmodulin-dependent protein kinase type II beta chain Short name=CaM-kinase II beta chain Short name=CaM kinase II subunit beta Short name=CaMK-II subunit beta EC=2.7.11.17 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 542 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Autophosphorylation of CAMK2 plays an important role in the regulation of the kinase activity. |
| Subunit structure | CAMK2 is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. Interacts with SYNGAP1, CAMK2N2 and MPDZ By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain. |
Ontologies
| Keywords | |
|---|---|
| Ligand | ATP-binding Calmodulin-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | G1/S transition of mitotic cell cycle Inferred from mutant phenotype. Source: MGI calcium ion transportInferred from mutant phenotype. Source: MGI protein amino acid autophosphorylationInferred from mutant phenotype. Source: MGI |
| Cellular component | calcium- and calmodulin-dependent protein kinase complex Traceable author statement. Source: UniProtKB |
| Molecular function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin bindingInferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 542 | 542 | Calcium/calmodulin-dependent protein kinase type II beta chain | PRO_0000086097 | |||||
Regions | |||||||||
| Domain | 14 – 272 | 259 | Protein kinase | ||||||
| Nucleotide binding | 20 – 28 | 9 | ATP By similarity | ||||||
| Region | 291 – 301 | 11 | Calmodulin-binding | ||||||
Sites | |||||||||
| Active site | 136 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 43 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 17 | 1 | Phosphotyrosine Ref.7 | ||||||
| Modified residue | 287 | 1 | Phosphothreonine Ref.6 Ref.8 | ||||||
| Modified residue | 315 | 1 | Phosphoserine Ref.5 | ||||||
| Modified residue | 367 | 1 | Phosphoserine Ref.8 | ||||||
| Modified residue | 397 | 1 | Phosphoserine Ref.8 | ||||||
Experimental info | |||||||||
| Sequence conflict | 19 | 1 | D → E in CAA45160. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Structure, expression, and chromosome location of the gene for the beta subunit of brain-specific Ca2+/calmodulin-dependent protein kinase II identified by transgene integration in an embryonic lethal mouse mutant." Karls U., Mueller U., Gilbert D.J., Copeland N.G., Jenkins N.A., Harbers K. Mol. Cell. Biol. 12:3644-3652(1992) [PubMed: 1321343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cerebellum. |
| [3] | "Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J. |
| [4] | Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 136-147, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus. |
| [5] | "Proteomic analysis of in vivo phosphorylated synaptic proteins." Collins M.O., Yu L., Coba M.P., Husi H., Campuzano I., Blackstock W.P., Choudhary J.S., Grant S.G. J. Biol. Chem. 280:5972-5982(2005) [PubMed: 15572359] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, MASS SPECTROMETRY. Tissue: Brain. |
| [6] | "Comprehensive identification of phosphorylation sites in postsynaptic density preparations." Trinidad J.C., Specht C.G., Thalhammer A., Schoepfer R., Burlingame A.L. Mol. Cell. Proteomics 5:914-922(2006) [PubMed: 16452087] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-287, MASS SPECTROMETRY. Tissue: Brain. |
| [7] | "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-17, MASS SPECTROMETRY. Tissue: Brain. |
| [8] | "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-367; THR-287 AND SER-397, MASS SPECTROMETRY. Tissue: Brain cortex. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| X63615 mRNA. Translation: CAA45160.1. AK163698 mRNA. Translation: BAE37464.1. AL611926, AL645469 Genomic DNA. Translation: CAI24948.1. AL645469, AL611926 Genomic DNA. Translation: CAI25258.1. | |
| IPI | IPI00474502. |
| PIR | A45025. |
| RefSeq | NP_031621.3. |
| UniGene | Mm.439733 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1HKX based on UniProtKB P11798. |
| SMR | P28652. Positions 8-315, 403-536. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P28652. 5 interactions. |
| STRING | P28652. |
PTM databases | |
| PhosphoSite | P28652. |
Proteomic databases | |
| PRIDE | P28652. |
Genome annotation databases | |
| Ensembl | ENSMUST00000019133; ENSMUSP00000019133; ENSMUSG00000057897; Mus musculus. [Genome view] ENSMUST00000109813; ENSMUSP00000105438; ENSMUSG00000057897; Mus musculus. [Genome view] ENSMUST00000109815; ENSMUSP00000105440; ENSMUSG00000057897; Mus musculus. [Genome view] |
| GeneID | 12323. |
| KEGG | mmu:12323. |
| UCSC | uc007hxr.1. mouse. |
Organism-specific databases | |
| CTD | 12323. |
| MGI | MGI:88257. Camk2b. |
Phylogenomic databases | |
| HOVERGEN | P28652. |
Enzyme and pathway databases | |
| BRENDA | 2.7.11.17. 244. |
Gene expression databases | |
| ArrayExpress | P28652. |
| Bgee | P28652. |
| Genevestigator | P28652. |
| GermOnline | ENSMUSG00000057897. Mus musculus. |
Family and domain databases | |
| InterPro | IPR015742. Ca/calmodulin-dep_kinase. IPR013543. Ca/calmodulin-dep_kinase-assoc. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| PANTHER | PTHR22982:SF64. CaMKII. 1 hit. |
| Pfam | PF08332. CaMKII_AD. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | KCC2B_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P28652 Secondary accession number(s): Q5SVH9 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


