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Protein

CD63 antigen

Gene

Cd63

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

GO - Biological processi

  • cell-matrix adhesion Source: UniProtKB
  • cell migration Source: UniProtKB
  • cell surface receptor signaling pathway Source: GO_Central
  • endosome to melanosome transport Source: UniProtKB
  • epithelial cell differentiation Source: RGD
  • negative regulation of epithelial cell migration Source: RGD
  • pigment granule maturation Source: UniProtKB
  • positive regulation of cell adhesion Source: RGD
  • positive regulation of endocytosis Source: RGD
  • positive regulation of integrin-mediated signaling pathway Source: UniProtKB
  • positive regulation of receptor internalization Source: UniProtKB
  • protein transport Source: UniProtKB-KW
  • regulation of vascular endothelial growth factor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
CD63 antigen
Alternative name(s):
Mast cell antigen AD1
CD_antigen: CD63
Gene namesi
Name:Cd63
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi62080. Cd63.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1110CytoplasmicSequence analysis
Transmembranei12 – 3221HelicalSequence analysisAdd
BLAST
Topological domaini33 – 5119ExtracellularSequence analysisAdd
BLAST
Transmembranei52 – 7221HelicalSequence analysisAdd
BLAST
Topological domaini73 – 819CytoplasmicSequence analysis
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 203101ExtracellularSequence analysisAdd
BLAST
Transmembranei204 – 22421HelicalSequence analysisAdd
BLAST
Topological domaini225 – 23814CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: RGD
  • endomembrane system Source: GO_Central
  • extracellular exosome Source: BHF-UCL
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
  • intrinsic component of plasma membrane Source: UniProtKB
  • late endosome membrane Source: UniProtKB
  • lysosomal membrane Source: UniProtKB
  • lysosome Source: RGD
  • melanosome Source: UniProtKB-SubCell
  • multivesicular body, internal vesicle Source: UniProtKB
  • plasma membrane Source: RGD
  • protein complex Source: RGD
  • transport vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endosome, Lysosome, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 238237CD63 antigenPRO_0000219219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi130 – 1301N-linked (GlcNAc...)Sequence analysis
Glycosylationi150 – 1501N-linked (GlcNAc...)Sequence analysis
Glycosylationi172 – 1721N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP28648.
PRIDEiP28648.

Expressioni

Tissue specificityi

Detected in mast cells and platelets (at protein level).1 Publication

Developmental stagei

Increased expression in embryonal tissues.

Gene expression databases

ExpressionAtlasiP28648. baseline and differential.
GenevisibleiP28648. RN.

Interactioni

Subunit structurei

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes. Interacts with SYT7 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PtpraQ033485EBI-7784314,EBI-7784341
SrcQ9WUD92EBI-7784314,EBI-7784541

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiP28648. 2 interactions.
STRINGi10116.ENSRNOP00000010180.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi234 – 2385Lysosomal targeting motifBy similarity

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00760000118875.
HOGENOMiHOG000230651.
HOVERGENiHBG107306.
InParanoidiP28648.
KOiK06497.
PhylomeDBiP28648.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEGGMKCV KFLLYVLLLA FCACAVGLIA IGVAVQVVLK QAITHETTAG
60 70 80 90 100
SLLPVVIIAV GAFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAV
110 120 130 140 150
AIAGYVFRDQ VKSEFSKSFQ KQMQNYLTDN KTATILDKLQ KENKCCGASN
160 170 180 190 200
YTDWERIPGM AKDRVPDSCC INITVGCGND FKESTIHTQG CVETIAAWLR
210 220 230
KNVLLVAGAA LGIAFVEVLG IIFSCCLVKS IRSGYEVM
Length:238
Mass (Da):25,699
Last modified:January 23, 2007 - v2
Checksum:iB6F8C8B69E766690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61654 mRNA. Translation: CAA43835.1.
BC063173 mRNA. Translation: AAH63173.1.
PIRiA46508.
RefSeqiNP_058821.1. NM_017125.3.
XP_008763247.1. XM_008765025.1.
UniGeneiRn.11068.

Genome annotation databases

EnsembliENSRNOT00000010180; ENSRNOP00000010180; ENSRNOG00000007650.
GeneIDi29186.
KEGGirno:29186.
UCSCiRGD:62080. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61654 mRNA. Translation: CAA43835.1.
BC063173 mRNA. Translation: AAH63173.1.
PIRiA46508.
RefSeqiNP_058821.1. NM_017125.3.
XP_008763247.1. XM_008765025.1.
UniGeneiRn.11068.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP28648. 2 interactions.
STRINGi10116.ENSRNOP00000010180.

Proteomic databases

PaxDbiP28648.
PRIDEiP28648.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000010180; ENSRNOP00000010180; ENSRNOG00000007650.
GeneIDi29186.
KEGGirno:29186.
UCSCiRGD:62080. rat.

Organism-specific databases

CTDi967.
RGDi62080. Cd63.

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00760000118875.
HOGENOMiHOG000230651.
HOVERGENiHBG107306.
InParanoidiP28648.
KOiK06497.
PhylomeDBiP28648.

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.

Miscellaneous databases

PROiP28648.

Gene expression databases

ExpressionAtlasiP28648. baseline and differential.
GenevisibleiP28648. RN.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD63_RAT
AccessioniPrimary (citable) accession number: P28648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.