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Protein

CD63 antigen

Gene

Cd63

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli (By similarity).By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-114608. Platelet degranulation.
R-RNO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
CD63 antigen
Alternative name(s):
Mast cell antigen AD1
CD_antigen: CD63
Gene namesi
Name:Cd63
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi62080. Cd63.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 11CytoplasmicSequence analysis10
Transmembranei12 – 32HelicalSequence analysisAdd BLAST21
Topological domaini33 – 51ExtracellularSequence analysisAdd BLAST19
Transmembranei52 – 72HelicalSequence analysisAdd BLAST21
Topological domaini73 – 81CytoplasmicSequence analysis9
Transmembranei82 – 102HelicalSequence analysisAdd BLAST21
Topological domaini103 – 203ExtracellularSequence analysisAdd BLAST101
Transmembranei204 – 224HelicalSequence analysisAdd BLAST21
Topological domaini225 – 238CytoplasmicSequence analysisAdd BLAST14

Keywords - Cellular componenti

Cell membrane, Endosome, Lysosome, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002192192 – 238CD63 antigenAdd BLAST237

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi130N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi150N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi172N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Palmitoylated at a low, basal level in unstimulated platelets. The level of palmitoylation increases when platelets are activated by thrombin (in vitro) (By similarity).By similarity

Keywords - PTMi

Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP28648.
PRIDEiP28648.

Expressioni

Tissue specificityi

Detected in mast cells and platelets (at protein level).1 Publication

Developmental stagei

Increased expression in embryonal tissues.

Gene expression databases

BgeeiENSRNOG00000007650.
ExpressionAtlasiP28648. baseline and differential.
GenevisibleiP28648. RN.

Interactioni

Subunit structurei

Interacts with TIMP1 and ITGB1 and recruits TIMP1 to ITGB1. Interacts with CD9. Identified in a complex with CD9 and ITGB3. Interacts with PMEL. Interacts with KDR/VEGFR2; identified in a complex with ITGB1 and KDR/VEGFR2 and is required to recruit KDR to ITGB1 complexes. Interacts with SYT7 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

IntActiP28648. 2 interactors.
STRINGi10116.ENSRNOP00000010180.

Structurei

3D structure databases

SMRiP28648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi234 – 238Lysosomal targeting motifBy similarity5

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3882. Eukaryota.
ENOG4111IRY. LUCA.
GeneTreeiENSGT00880000137851.
HOGENOMiHOG000230651.
HOVERGENiHBG107306.
InParanoidiP28648.
KOiK06497.
PhylomeDBiP28648.

Family and domain databases

Gene3Di1.10.1450.10. 1 hit.
InterProiView protein in InterPro
IPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2_sf.
PfamiView protein in Pfam
PF00335. Tetraspannin. 1 hit.
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiView protein in PROSITE
PS00421. TM4_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28648-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVEGGMKCV KFLLYVLLLA FCACAVGLIA IGVAVQVVLK QAITHETTAG
60 70 80 90 100
SLLPVVIIAV GAFLFLVAFV GCCGACKENY CLMITFAIFL SLIMLVEVAV
110 120 130 140 150
AIAGYVFRDQ VKSEFSKSFQ KQMQNYLTDN KTATILDKLQ KENKCCGASN
160 170 180 190 200
YTDWERIPGM AKDRVPDSCC INITVGCGND FKESTIHTQG CVETIAAWLR
210 220 230
KNVLLVAGAA LGIAFVEVLG IIFSCCLVKS IRSGYEVM
Length:238
Mass (Da):25,699
Last modified:January 23, 2007 - v2
Checksum:iB6F8C8B69E766690
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X61654 mRNA. Translation: CAA43835.1.
BC063173 mRNA. Translation: AAH63173.1.
PIRiA46508.
RefSeqiNP_058821.1. NM_017125.3.
XP_008763247.1. XM_008765025.2.
UniGeneiRn.11068.

Genome annotation databases

EnsembliENSRNOT00000010180; ENSRNOP00000010180; ENSRNOG00000007650.
GeneIDi29186.
KEGGirno:29186.
UCSCiRGD:62080. rat.

Similar proteinsi

Entry informationi

Entry nameiCD63_RAT
AccessioniPrimary (citable) accession number: P28648
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 22, 2017
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families