P28647 (AA3R_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A3

Alternative name(s):
TGPCR1

Gene names

Name:

Adora3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Testis, particularly in spermatocytes and spermatids but not in spermatogonia. Low levels in the brain.
Developmental stage	Expressed during spermiogenesis.
Post-translational modification	Phosphorylation on Thr-318 and Thr-319 may be crucial for rapid desensitization. Phosphorylation on Thr-318 may be necessary for phosphorylation on Thr-319 to occur.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	regulation of norepinephrine secretion Inferred from mutant phenotype. Source: RGD
Cellular component	presynaptic membrane Inferred from direct assay. Source: RGD
Molecular function	G-protein coupled adenosine receptor activity Inferred from direct assay Ref.1. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P28647-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform B (identifier: P28647-2)

Also known as: A3i;

The sequence of this isoform differs from the canonical sequence as follows:
     119-119: R → RLSFLVVNLPFSSPHSNR

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Adenosine receptor A3		PRO_0000069013
Regions
	Topological domain	1 – 16	16	Extracellular By similarity
	Transmembrane	17 – 39	23	Helical; Name=1; By similarity
	Topological domain	40 – 50	11	Cytoplasmic By similarity
	Transmembrane	51 – 74	24	Helical; Name=2; By similarity
	Topological domain	75 – 86	12	Extracellular By similarity
	Transmembrane	87 – 108	22	Helical; Name=3; By similarity
	Topological domain	109 – 128	20	Cytoplasmic By similarity
	Transmembrane	129 – 150	22	Helical; Name=4; By similarity
	Topological domain	151 – 179	29	Extracellular By similarity
	Transmembrane	180 – 200	21	Helical; Name=5; By similarity
	Topological domain	201 – 233	33	Cytoplasmic By similarity
	Transmembrane	234 – 257	24	Helical; Name=6; By similarity
	Topological domain	258 – 263	6	Extracellular By similarity
	Transmembrane	264 – 286	23	Helical; Name=7; By similarity
	Topological domain	287 – 320	34	Cytoplasmic By similarity
Amino acid modifications
	Modified residue	307	1	Phosphothreonine Probable
	Modified residue	318	1	Phosphothreonine Probable
	Modified residue	319	1	Phosphothreonine Probable
	Lipidation	305	1	S-palmitoyl cysteine Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	5	1	N-linked (GlcNAc...) Potential
	Disulfide bond	85 ↔ 168		By similarity
Natural variations
	Alternative sequence	119	1	R → RLSFLVVNLPFSSPHSNR in isoform B.		VSP_001859
Experimental info
	Mutagenesis	302 – 305	4	CRLC → ARLA: Promotes agonist-independent phosphorylation. Ref.4
	Mutagenesis	307	1	T → A: Small decrease in agonist-stimulated phosphorylation. Ref.4
	Mutagenesis	318	1	T → A or E: Strong decrease in agonist-stimulated phosphorylation. Ref.4
	Mutagenesis	319	1	T → A: 50% decrease in agonist-stimulated phosphorylation. Ref.4
	Sequence conflict	18	1	I → V in AAA40680. Ref.1
	Sequence conflict	68	1	I → V in CAA63702. Ref.3
	Sequence conflict	74 – 83	10	VSLEVQMHFY → SAWRSRCTSM in CAA41937. Ref.2
	Sequence conflict	132	1	F → L in CAA63702. Ref.3
	Sequence conflict	172	1	S → F in AAA40680. Ref.1
	Sequence conflict	288	1	I → N Ref.1
	Sequence conflict	291 – 295	5	FKETY → VQRNH Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform A [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 59DBA40C594ACDAB	FASTA	320	36,629
	10 20 30 40 50 60 MKANNTTTSA LWLQITYITM EAAIGLCAVV GNMLVIWVVK LNRTLRTTTF YFIVSLALAD 70 80 90 100 110 120 IAVGVLVIPL AIAVSLEVQM HFYACLFMSC VLLVFTHASI MSLLAIAVDR YLRVKLTVRY 130 140 150 160 170 180 RTVTTQRRIW LFLGLCWLVS FLVGLTPMFG WNRKVTLELS QNSSTLSCHF RSVVGLDYMV 190 200 210 220 230 240 FFSFITWILI PLVVMCIIYL DIFYIIRNKL SQNLTGFRET RAFYGREFKT AKSLFLVLFL 250 260 270 280 290 300 FALCWLPLSI INFVSYFNVK IPEIAMCLGI LLSHANSMMN PIVYACKIKK FKETYFVILR 310 320 ACRLCQTSDS LDSNLEQTTE
	Isoform B (A3i) [UniParc]. Checksum: 80350BBDE3302AA6	FASTA	337	38,525
	10 20 30 40 50 60 MKANNTTTSA LWLQITYITM EAAIGLCAVV GNMLVIWVVK LNRTLRTTTF YFIVSLALAD 70 80 90 100 110 120 IAVGVLVIPL AIAVSLEVQM HFYACLFMSC VLLVFTHASI MSLLAIAVDR YLRVKLTVRL 130 140 150 160 170 180 SFLVVNLPFS SPHSNRYRTV TTQRRIWLFL GLCWLVSFLV GLTPMFGWNR KVTLELSQNS 190 200 210 220 230 240 STLSCHFRSV VGLDYMVFFS FITWILIPLV VMCIIYLDIF YIIRNKLSQN LTGFRETRAF 250 260 270 280 290 300 YGREFKTAKS LFLVLFLFAL CWLPLSIINF VSYFNVKIPE IAMCLGILLS HANSMMNPIV 310 320 330 YACKIKKFKE TYFVILRACR LCQTSDSLDS NLEQTTE

References

[1]	"Molecular cloning and characterization of an adenosine receptor: the A3 adenosine receptor." Zhou Q.Y., Li C.Y., Olah M.E., Johnson R.A., Stiles G.L., Civelli O. Proc. Natl. Acad. Sci. U.S.A. 89:7432-7436(1992) [PubMed: 1323836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), CHARACTERIZATION. Tissue: Brain.
[2]	"Molecular cloning of a novel putative G-protein coupled receptor expressed during rat spermiogenesis." Meyerhof W., Mueller-Brechlin R., Richter D. FEBS Lett. 284:155-160(1991) [PubMed: 1647979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A). Strain: Wistar. Tissue: Testis.
[3]	"cDNA cloning and characterization of A3i, an alternatively spliced rat A3 adenosine receptor variant." Sajjadi F.G., Boyle D.L., Domingo R.C., Firestein G.S. FEBS Lett. 382:125-129(1996) [PubMed: 8612733] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B). Tissue: Brain.
[4]	"Identification of threonine residues controlling the agonist-dependent phosphorylation and desensitization of the rat A(3) adenosine receptor." Palmer T.M., Stiles G.L. Mol. Pharmacol. 57:539-545(2000) [PubMed: 10692494] [Abstract] Cited for: PHOSPHORYLATION AT THR-307; THR-318 AND THR-319, MUTAGENESIS OF 302-CYS--CYS-305; THR-307; THR-318 AND THR-319.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59249 mRNA. Translation: CAA41937.1. M94152 mRNA. Translation: AAA40680.1. X93219 mRNA. Translation: CAA63702.1.
IPI	IPI00202795. IPI00231943.
PIR	A46152. S17177. S68678.
RefSeq	NP_037028.2. NM_012896.2.
UniGene	Rn.10457. Rn.205912.
3D structure databases
ProteinModelPortal	P28647.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-217N.
STRING	P28647.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P28647.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021236; ENSRNOP00000021236; ENSRNOG00000015788.
GeneID	25370.
KEGG	rno:25370.
UCSC	NM_012896. rat.
Organism-specific databases
CTD	140.
RGD	2051. Adora3.
Phylogenomic databases
eggNOG	roNOG14435.
HOVERGEN	HBG106962.
OMA	SRIRTEE.
OrthoDB	EOG4MKNH1.
PhylomeDB	P28647.
Gene expression databases
ArrayExpress	P28647.
Genevestigator	P28647.
GermOnline	ENSRNOG00000015788. Rattus norvegicus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR000466. Adeno_A3_rcpt. IPR001634. Adenosn_rcpt. IPR017452. GPCR_Rhodpsn_supfam. [Graphical view]
KO	K04268.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00555. ADENOSINEA3R. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606381.

Entry information

Entry name

AA3R_RAT

Accession

Primary (citable) accession number: P28647
Secondary accession number(s): Q63792

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 102 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program