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Protein

Adenosine receptor A3

Gene

Adora3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase. Possible role in reproduction.

GO - Molecular functioni

  • G-protein coupled adenosine receptor activity Source: RGD

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: RGD
  • regulation of norepinephrine secretion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-417973. Adenosine P1 receptors.
R-RNO-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A3
Alternative name(s):
TGPCR1
Gene namesi
Name:Adora3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 2

Organism-specific databases

RGDi2051. Adora3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1616ExtracellularBy similarityAdd
BLAST
Transmembranei17 – 3923Helical; Name=1By similarityAdd
BLAST
Topological domaini40 – 5011CytoplasmicBy similarityAdd
BLAST
Transmembranei51 – 7424Helical; Name=2By similarityAdd
BLAST
Topological domaini75 – 8612ExtracellularBy similarityAdd
BLAST
Transmembranei87 – 10822Helical; Name=3By similarityAdd
BLAST
Topological domaini109 – 12820CytoplasmicBy similarityAdd
BLAST
Transmembranei129 – 15022Helical; Name=4By similarityAdd
BLAST
Topological domaini151 – 17929ExtracellularBy similarityAdd
BLAST
Transmembranei180 – 20021Helical; Name=5By similarityAdd
BLAST
Topological domaini201 – 23333CytoplasmicBy similarityAdd
BLAST
Transmembranei234 – 25724Helical; Name=6By similarityAdd
BLAST
Topological domaini258 – 2636ExtracellularBy similarity
Transmembranei264 – 28623Helical; Name=7By similarityAdd
BLAST
Topological domaini287 – 32034CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • presynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi302 – 3054CRLC → ARLA: Promotes agonist-independent phosphorylation. 1 Publication
Mutagenesisi307 – 3071T → A: Small decrease in agonist-stimulated phosphorylation. 1 Publication
Mutagenesisi318 – 3181T → A or E: Strong decrease in agonist-stimulated phosphorylation. 1 Publication
Mutagenesisi319 – 3191T → A: 50% decrease in agonist-stimulated phosphorylation. 1 Publication

Chemistry

ChEMBLiCHEMBL2111386.
GuidetoPHARMACOLOGYi21.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 320320Adenosine receptor A3PRO_0000069013Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi4 – 41N-linked (GlcNAc...)Sequence analysis
Glycosylationi5 – 51N-linked (GlcNAc...)Sequence analysis
Disulfide bondi85 ↔ 168PROSITE-ProRule annotation
Lipidationi305 – 3051S-palmitoyl cysteineSequence analysis
Modified residuei307 – 3071Phosphothreonine1 Publication
Modified residuei318 – 3181Phosphothreonine1 Publication
Modified residuei319 – 3191Phosphothreonine1 Publication

Post-translational modificationi

Phosphorylation on Thr-318 and Thr-319 may be crucial for rapid desensitization. Phosphorylation on Thr-318 may be necessary for phosphorylation on Thr-319 to occur.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP28647.

PTM databases

iPTMnetiP28647.
PhosphoSiteiP28647.

Expressioni

Tissue specificityi

Testis, particularly in spermatocytes and spermatids but not in spermatogonia. Low levels in the brain.

Developmental stagei

Expressed during spermiogenesis.

Gene expression databases

GenevisibleiP28647. RN.

Interactioni

Protein-protein interaction databases

DIPiDIP-217N.
STRINGi10116.ENSRNOP00000046340.

Chemistry

BindingDBiP28647.

Structurei

3D structure databases

ProteinModelPortaliP28647.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiP28647.
KOiK04268.
OMAiETYLLIF.
OrthoDBiEOG79SDXQ.
PhylomeDBiP28647.
TreeFamiTF325296.

Family and domain databases

InterProiIPR000466. Adeno_A3_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00555. ADENOSINEA3R.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P28647-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKANNTTTSA LWLQITYITM EAAIGLCAVV GNMLVIWVVK LNRTLRTTTF
60 70 80 90 100
YFIVSLALAD IAVGVLVIPL AIAVSLEVQM HFYACLFMSC VLLVFTHASI
110 120 130 140 150
MSLLAIAVDR YLRVKLTVRY RTVTTQRRIW LFLGLCWLVS FLVGLTPMFG
160 170 180 190 200
WNRKVTLELS QNSSTLSCHF RSVVGLDYMV FFSFITWILI PLVVMCIIYL
210 220 230 240 250
DIFYIIRNKL SQNLTGFRET RAFYGREFKT AKSLFLVLFL FALCWLPLSI
260 270 280 290 300
INFVSYFNVK IPEIAMCLGI LLSHANSMMN PIVYACKIKK FKETYFVILR
310 320
ACRLCQTSDS LDSNLEQTTE
Length:320
Mass (Da):36,629
Last modified:November 1, 1997 - v3
Checksum:i59DBA40C594ACDAB
GO
Isoform B (identifier: P28647-2) [UniParc]FASTAAdd to basket

Also known as: A3i

The sequence of this isoform differs from the canonical sequence as follows:
     119-119: R → RLSFLVVNLPFSSPHSNR

Show »
Length:337
Mass (Da):38,525
Checksum:i80350BBDE3302AA6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181I → V in AAA40680 (PubMed:1323836).Curated
Sequence conflicti68 – 681I → V in CAA63702 (PubMed:8612733).Curated
Sequence conflicti74 – 8310VSLEVQMHFY → SAWRSRCTSM in CAA41937 (PubMed:1647979).Curated
Sequence conflicti132 – 1321F → L in CAA63702 (PubMed:8612733).Curated
Sequence conflicti172 – 1721S → F in AAA40680 (PubMed:1323836).Curated
Sequence conflicti288 – 2881I → N (PubMed:1323836).Curated
Sequence conflicti291 – 2955FKETY → VQRNH (PubMed:1323836).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei119 – 1191R → RLSFLVVNLPFSSPHSNR in isoform B. 1 PublicationVSP_001859

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59249 mRNA. Translation: CAA41937.1.
M94152 mRNA. Translation: AAA40680.1.
X93219 mRNA. Translation: CAA63702.1.
PIRiA46152.
S17177.
S68678.
RefSeqiNP_001289679.1. NM_001302750.1. [P28647-2]
NP_001289680.1. NM_001302751.1. [P28647-1]
NP_001289684.1. NM_001302755.1. [P28647-2]
NP_037028.2. NM_012896.3. [P28647-1]
UniGeneiRn.10457.
Rn.188812.

Genome annotation databases

EnsembliENSRNOT00000021236; ENSRNOP00000021236; ENSRNOG00000015788. [P28647-1]
ENSRNOT00000050538; ENSRNOP00000046340; ENSRNOG00000050743. [P28647-1]
GeneIDi100911796.
25370.
KEGGirno:100911796.
rno:25370.
UCSCiRGD:2051. rat. [P28647-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59249 mRNA. Translation: CAA41937.1.
M94152 mRNA. Translation: AAA40680.1.
X93219 mRNA. Translation: CAA63702.1.
PIRiA46152.
S17177.
S68678.
RefSeqiNP_001289679.1. NM_001302750.1. [P28647-2]
NP_001289680.1. NM_001302751.1. [P28647-1]
NP_001289684.1. NM_001302755.1. [P28647-2]
NP_037028.2. NM_012896.3. [P28647-1]
UniGeneiRn.10457.
Rn.188812.

3D structure databases

ProteinModelPortaliP28647.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-217N.
STRINGi10116.ENSRNOP00000046340.

Chemistry

BindingDBiP28647.
ChEMBLiCHEMBL2111386.
GuidetoPHARMACOLOGYi21.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP28647.
PhosphoSiteiP28647.

Proteomic databases

PaxDbiP28647.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021236; ENSRNOP00000021236; ENSRNOG00000015788. [P28647-1]
ENSRNOT00000050538; ENSRNOP00000046340; ENSRNOG00000050743. [P28647-1]
GeneIDi100911796.
25370.
KEGGirno:100911796.
rno:25370.
UCSCiRGD:2051. rat. [P28647-1]

Organism-specific databases

CTDi140.
RGDi2051. Adora3.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiP28647.
KOiK04268.
OMAiETYLLIF.
OrthoDBiEOG79SDXQ.
PhylomeDBiP28647.
TreeFamiTF325296.

Enzyme and pathway databases

ReactomeiR-RNO-417973. Adenosine P1 receptors.
R-RNO-418594. G alpha (i) signalling events.

Miscellaneous databases

NextBioi606381.
PROiP28647.

Gene expression databases

GenevisibleiP28647. RN.

Family and domain databases

InterProiIPR000466. Adeno_A3_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00555. ADENOSINEA3R.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of an adenosine receptor: the A3 adenosine receptor."
    Zhou Q.Y., Li C.Y., Olah M.E., Johnson R.A., Stiles G.L., Civelli O.
    Proc. Natl. Acad. Sci. U.S.A. 89:7432-7436(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), CHARACTERIZATION.
    Tissue: Brain.
  2. "Molecular cloning of a novel putative G-protein coupled receptor expressed during rat spermiogenesis."
    Meyerhof W., Mueller-Brechlin R., Richter D.
    FEBS Lett. 284:155-160(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
    Strain: Wistar.
    Tissue: Testis.
  3. "cDNA cloning and characterization of A3i, an alternatively spliced rat A3 adenosine receptor variant."
    Sajjadi F.G., Boyle D.L., Domingo R.C., Firestein G.S.
    FEBS Lett. 382:125-129(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B).
    Tissue: Brain.
  4. "Identification of threonine residues controlling the agonist-dependent phosphorylation and desensitization of the rat A(3) adenosine receptor."
    Palmer T.M., Stiles G.L.
    Mol. Pharmacol. 57:539-545(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-307; THR-318 AND THR-319, MUTAGENESIS OF 302-CYS--CYS-305; THR-307; THR-318 AND THR-319.

Entry informationi

Entry nameiAA3R_RAT
AccessioniPrimary (citable) accession number: P28647
Secondary accession number(s): Q63792
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: November 1, 1997
Last modified: May 11, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.