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Protein

3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic

Gene

CLKR27

Organism
Cuphea lanceolata (Cigar flower)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei214 – 2141SubstrateBy similarity
Active sitei227 – 2271Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi82 – 10625NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Source: UniProtKB-EC
  2. NAD binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic (EC:1.1.1.100)
Alternative name(s):
3-ketoacyl-acyl carrier protein reductase
Gene namesi
Name:CLKR27
OrganismiCuphea lanceolata (Cigar flower)
Taxonomic identifieri3930 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsMyrtalesLythraceaeCuphea

Subcellular locationi

Plastidchloroplast. Plastid
Note: And non-photosynthetic plastids.

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 6161ChloroplastBy similarityAdd
BLAST
Chaini62 – 3202593-oxoacyl-[acyl-carrier-protein] reductase, chloroplasticPRO_0000031981Add
BLAST

Interactioni

Subunit structurei

Homotetramer.Curated

Structurei

3D structure databases

ProteinModelPortaliP28643.
SMRiP28643. Positions 77-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28643-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MATATAAGCS GAVALKSLGG RRLCIPQQLS PVLAGFGSHA AKSFPILSTR
60 70 80 90 100
SIATSGIRAQ VATAEKVSAG AGQSVESPVV IVTGASRGIG KAIALSLGKA
110 120 130 140 150
GCKVLVNYAR SSKEAEEVSK EIEAFGGQAL TFGGDVSKEE DVEAMIKTAV
160 170 180 190 200
DAWGTVDILV NNAGITRDGL LMRMKKSQWQ EVIDLNLTGV FLCTQAAAKI
210 220 230 240 250
MMKKKKGRII NIASVVGLVG NAGQANYSAA KAGVIGFTKT VAREYASRNI
260 270 280 290 300
NVNAVAPGFI SSDMTSKLGD DINKKILETI PLGRYGQPEE VAGLVEFLAI
310 320
NPASSYVTGQ VFTIDGGMTM
Length:320
Mass (Da):33,103
Last modified:December 1, 1992 - v1
Checksum:i06BAF0522B2B8C87
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64566 mRNA. Translation: CAA45866.1.
PIRiS22450.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64566 mRNA. Translation: CAA45866.1.
PIRiS22450.

3D structure databases

ProteinModelPortaliP28643.
SMRiP28643. Positions 77-320.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PIRSFiPIRSF000126. 11-beta-HSD1. 1 hit.
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
TIGRFAMsiTIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a cDNA from Cuphea lanceolata encoding a beta-ketoacyl-ACP reductase."
    Klein B., Pawlowski K., Hoericke-Grandpierre C., Schell J., Toepfer R.
    Mol. Gen. Genet. 233:122-128(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiFABG_CUPLA
AccessioniPrimary (citable) accession number: P28643
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: January 7, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.