Reviewed, UniProtKB/Swiss-Prot P28643 (FABG_CUPLA)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase

Gene names

Name:

CLKR27

Organism

Cuphea lanceolata

Taxonomic identifier

3930 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › Myrtales › Lythraceae › Cuphea

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer Probable.
Subcellular location	Plastid › chloroplast. Plastid. Note: And non-photosynthetic plastids.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 61	61	Chloroplast By similarity
	Chain	62 – 320	259	3-oxoacyl-[acyl-carrier-protein] reductase, chloroplastic		PRO_0000031981
Regions
	Nucleotide binding	82 – 106	25	NADP By similarity
Sites
	Active site	227	1	Proton acceptor By similarity
	Binding site	214	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P28643-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 06BAF0522B2B8C87	FASTA	320	33,103
	10 20 30 40 50 60 MATATAAGCS GAVALKSLGG RRLCIPQQLS PVLAGFGSHA AKSFPILSTR SIATSGIRAQ 70 80 90 100 110 120 VATAEKVSAG AGQSVESPVV IVTGASRGIG KAIALSLGKA GCKVLVNYAR SSKEAEEVSK 130 140 150 160 170 180 EIEAFGGQAL TFGGDVSKEE DVEAMIKTAV DAWGTVDILV NNAGITRDGL LMRMKKSQWQ 190 200 210 220 230 240 EVIDLNLTGV FLCTQAAAKI MMKKKKGRII NIASVVGLVG NAGQANYSAA KAGVIGFTKT 250 260 270 280 290 300 VAREYASRNI NVNAVAPGFI SSDMTSKLGD DINKKILETI PLGRYGQPEE VAGLVEFLAI 310 320 NPASSYVTGQ VFTIDGGMTM

References

[1]	"Isolation and characterization of a cDNA from Cuphea lanceolata encoding a beta-ketoacyl-ACP reductase." Klein B., Pawlowski K., Hoericke-Grandpierre C., Schell J., Toepfer R. Mol. Gen. Genet. 233:122-128(1992) [PubMed: 1376402] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64566 mRNA. Translation: CAA45866.1.
PIR	S22450.
3D structure databases
HSSP	HSSP built from PDB template 1EDO based on UniProtKB Q93X62.
SMR	P28643. Positions 77-320.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.1.1.100. 66873.
Family and domain databases
InterPro	IPR011284. 3oxo_ACP_reduc. IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
TIGRFAMs	TIGR01830. 3oxo_ACP_reduc. 1 hit.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG_CUPLA

Accession

Primary (citable) accession number: P28643

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)