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P28638

- YHDJ_ECOLI

UniProt

P28638 - YHDJ_ECOLI

Protein

DNA adenine methyltransferase YhdJ

Gene

yhdJ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 3 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3').1 Publication

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.1 Publication

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. N-methyltransferase activity Source: InterPro
    3. site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoCyc

    GO - Biological processi

    1. DNA methylation on adenine Source: GOC

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11498-MONOMER.
    ECOL316407:JW5543-MONOMER.
    MetaCyc:EG11498-MONOMER.

    Protein family/group databases

    REBASEi3885. M.EcoKII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA adenine methyltransferase YhdJ (EC:2.1.1.72)
    Gene namesi
    Name:yhdJ
    Ordered Locus Names:b3262, JW5543
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11498. yhdJ.

    Pathology & Biotechi

    Disruption phenotypei

    Mutants are viable and show no significant growth deficiency and no alteration of cell morphology.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 294294DNA adenine methyltransferase YhdJPRO_0000088000Add
    BLAST

    Proteomic databases

    PRIDEiP28638.

    Expressioni

    Gene expression databases

    GenevestigatoriP28638.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-12296N.
    IntActiP28638. 34 interactions.
    STRINGi511145.b3262.

    Structurei

    3D structure databases

    ProteinModelPortaliP28638.
    SMRiP28638. Positions 26-249.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Phylogenomic databases

    eggNOGiCOG0863.
    HOGENOMiHOG000125330.
    KOiK07319.
    OMAiRQETRMV.
    OrthoDBiEOG6FV84T.
    PhylomeDBiP28638.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    InterProiIPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF01555. N6_N4_Mtase. 1 hit.
    [Graphical view]
    PRINTSiPR00508. S21N4MTFRASE.
    SUPFAMiSSF53335. SSF53335. 1 hit.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P28638-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTGCEPTRF GNEAKTIIHG DALAELKKIP AESVDLIFAD PPYNIGKNFD    50
    GLIEAWKEDL FIDWLFEVIA ECHRVLKKQG SMYIMNSTEN MPFIDLQCRK 100
    LFTIKSRIVW SYDSSGVQAK KHYGSMYEPI LMMVKDAKNY TFNGDAILVE 150
    AKTGSQRALI DYRKNPPQPY NHQKVPGNVW DFPRVRYLMD EYENHPTQKP 200
    EALLKRIILA SSNPGDIVLD PFAGSFTTGA VAIASGRKFI GIEINSEYIK 250
    MGLRRLDVAS HYSAEELAKV KKRKTGNLSK RSRLSEVDPD LITK 294
    Length:294
    Mass (Da):33,397
    Last modified:December 15, 1998 - v3
    Checksum:i6BCC49D9A571884B
    GO

    Sequence cautioni

    The sequence AAA58066.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti28 – 281K → N in AAA23784. (PubMed:1459953)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18997 Genomic DNA. Translation: AAA58066.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76294.2.
    AP009048 Genomic DNA. Translation: BAE77303.1.
    M95784 Genomic DNA. Translation: AAA23784.1.
    J03245 Genomic DNA. Translation: AAA83857.1.
    PIRiH65118.
    RefSeqiNP_417728.4. NC_000913.3.
    YP_491444.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76294; AAC76294; b3262.
    BAE77303; BAE77303; BAE77303.
    GeneIDi12933456.
    947695.
    KEGGiecj:Y75_p3180.
    eco:b3262.
    PATRICi32121954. VBIEscCol129921_3361.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U18997 Genomic DNA. Translation: AAA58066.1 . Different initiation.
    U00096 Genomic DNA. Translation: AAC76294.2 .
    AP009048 Genomic DNA. Translation: BAE77303.1 .
    M95784 Genomic DNA. Translation: AAA23784.1 .
    J03245 Genomic DNA. Translation: AAA83857.1 .
    PIRi H65118.
    RefSeqi NP_417728.4. NC_000913.3.
    YP_491444.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P28638.
    SMRi P28638. Positions 26-249.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-12296N.
    IntActi P28638. 34 interactions.
    STRINGi 511145.b3262.

    Protein family/group databases

    REBASEi 3885. M.EcoKII.

    Proteomic databases

    PRIDEi P28638.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76294 ; AAC76294 ; b3262 .
    BAE77303 ; BAE77303 ; BAE77303 .
    GeneIDi 12933456.
    947695.
    KEGGi ecj:Y75_p3180.
    eco:b3262.
    PATRICi 32121954. VBIEscCol129921_3361.

    Organism-specific databases

    EchoBASEi EB1461.
    EcoGenei EG11498. yhdJ.

    Phylogenomic databases

    eggNOGi COG0863.
    HOGENOMi HOG000125330.
    KOi K07319.
    OMAi RQETRMV.
    OrthoDBi EOG6FV84T.
    PhylomeDBi P28638.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11498-MONOMER.
    ECOL316407:JW5543-MONOMER.
    MetaCyc:EG11498-MONOMER.

    Miscellaneous databases

    PROi P28638.

    Gene expression databases

    Genevestigatori P28638.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    InterProi IPR002941. DNA_methylase_N4/N6.
    IPR002052. DNA_methylase_N6_adenine_CS.
    IPR001091. RM_Methylase.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF01555. N6_N4_Mtase. 1 hit.
    [Graphical view ]
    PRINTSi PR00508. S21N4MTFRASE.
    SUPFAMi SSF53335. SSF53335. 1 hit.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. "Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
      Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
      J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
    4. "Isolation of the gene encoding the Hin recombinational enhancer binding protein."
      Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
      Strain: K12.
    5. "YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica."
      Broadbent S.E., Balbontin R., Casadesus J., Marinus M.G., van der Woude M.
      J. Bacteriol. 189:4325-4327(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiYHDJ_ECOLI
    AccessioniPrimary (citable) accession number: P28638
    Secondary accession number(s): Q2M8V3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 1992
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 116 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3