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P28638 (YHDJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA adenine methyltransferase YhdJ

EC=2.1.1.72
Gene names
Name:yhdJ
Ordered Locus Names:b3262, JW5543
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length294 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3'). Ref.5

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine. Ref.5

Disruption phenotype

Mutants are viable and show no significant growth deficiency and no alteration of cell morphology. Ref.5

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence caution

The sequence AAA58066.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 294294DNA adenine methyltransferase YhdJ
PRO_0000088000

Experimental info

Sequence conflict281K → N in AAA23784. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P28638 [UniParc].

Last modified December 15, 1998. Version 3.
Checksum: 6BCC49D9A571884B

FASTA29433,397
        10         20         30         40         50         60 
MRTGCEPTRF GNEAKTIIHG DALAELKKIP AESVDLIFAD PPYNIGKNFD GLIEAWKEDL 

        70         80         90        100        110        120 
FIDWLFEVIA ECHRVLKKQG SMYIMNSTEN MPFIDLQCRK LFTIKSRIVW SYDSSGVQAK 

       130        140        150        160        170        180 
KHYGSMYEPI LMMVKDAKNY TFNGDAILVE AKTGSQRALI DYRKNPPQPY NHQKVPGNVW 

       190        200        210        220        230        240 
DFPRVRYLMD EYENHPTQKP EALLKRIILA SSNPGDIVLD PFAGSFTTGA VAIASGRKFI 

       250        260        270        280        290 
GIEINSEYIK MGLRRLDVAS HYSAEELAKV KKRKTGNLSK RSRLSEVDPD LITK 

« Hide

References

« Hide 'large scale' references
[1]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[4]"Isolation of the gene encoding the Hin recombinational enhancer binding protein."
Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Strain: K12.
[5]"YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica."
Broadbent S.E., Balbontin R., Casadesus J., Marinus M.G., van der Woude M.
J. Bacteriol. 189:4325-4327(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18997 Genomic DNA. Translation: AAA58066.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76294.2.
AP009048 Genomic DNA. Translation: BAE77303.1.
M95784 Genomic DNA. Translation: AAA23784.1.
J03245 Genomic DNA. Translation: AAA83857.1.
PIRH65118.
RefSeqNP_417728.4. NC_000913.3.
YP_491444.1. NC_007779.1.

3D structure databases

ProteinModelPortalP28638.
SMRP28638. Positions 26-249.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-12296N.
IntActP28638. 33 interactions.
STRING511145.b3262.

Protein family/group databases

REBASE3885. M.EcoKII.

Proteomic databases

PRIDEP28638.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76294; AAC76294; b3262.
BAE77303; BAE77303; BAE77303.
GeneID12933456.
947695.
KEGGecj:Y75_p3180.
eco:b3262.
PATRIC32121954. VBIEscCol129921_3361.

Organism-specific databases

EchoBASEEB1461.
EcoGeneEG11498. yhdJ.

Phylogenomic databases

eggNOGCOG0863.
HOGENOMHOG000125330.
KOK07319.
OMAIASAPRM.
OrthoDBEOG6FV84T.
PhylomeDBP28638.
ProtClustDBPRK11524.

Enzyme and pathway databases

BioCycEcoCyc:EG11498-MONOMER.
ECOL316407:JW5543-MONOMER.
MetaCyc:EG11498-MONOMER.

Gene expression databases

GenevestigatorP28638.

Family and domain databases

InterProIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
[Graphical view]
PfamPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSPR00508. S21N4MTFRASE.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP28638.

Entry information

Entry nameYHDJ_ECOLI
AccessionPrimary (citable) accession number: P28638
Secondary accession number(s): Q2M8V3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene