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P28638

- YHDJ_ECOLI

UniProt

P28638 - YHDJ_ECOLI

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Protein

DNA adenine methyltransferase YhdJ

Gene

yhdJ

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3').1 Publication

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.1 Publication

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. N-methyltransferase activity Source: InterPro
  3. site-specific DNA-methyltransferase (adenine-specific) activity Source: EcoCyc

GO - Biological processi

  1. DNA methylation on adenine Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11498-MONOMER.
ECOL316407:JW5543-MONOMER.
MetaCyc:EG11498-MONOMER.

Protein family/group databases

REBASEi3885. M.EcoKII.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA adenine methyltransferase YhdJ (EC:2.1.1.72)
Gene namesi
Name:yhdJ
Ordered Locus Names:b3262, JW5543
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11498. yhdJ.

Pathology & Biotechi

Disruption phenotypei

Mutants are viable and show no significant growth deficiency and no alteration of cell morphology.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 294294DNA adenine methyltransferase YhdJPRO_0000088000Add
BLAST

Proteomic databases

PRIDEiP28638.

Expressioni

Gene expression databases

GenevestigatoriP28638.

Interactioni

Protein-protein interaction databases

DIPiDIP-12296N.
IntActiP28638. 34 interactions.
STRINGi511145.b3262.

Structurei

3D structure databases

ProteinModelPortaliP28638.
SMRiP28638. Positions 26-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Phylogenomic databases

eggNOGiCOG0863.
HOGENOMiHOG000125330.
InParanoidiP28638.
KOiK07319.
OMAiRQETRMV.
OrthoDBiEOG6FV84T.
PhylomeDBiP28638.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF01555. N6_N4_Mtase. 1 hit.
[Graphical view]
PRINTSiPR00508. S21N4MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28638-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRTGCEPTRF GNEAKTIIHG DALAELKKIP AESVDLIFAD PPYNIGKNFD
60 70 80 90 100
GLIEAWKEDL FIDWLFEVIA ECHRVLKKQG SMYIMNSTEN MPFIDLQCRK
110 120 130 140 150
LFTIKSRIVW SYDSSGVQAK KHYGSMYEPI LMMVKDAKNY TFNGDAILVE
160 170 180 190 200
AKTGSQRALI DYRKNPPQPY NHQKVPGNVW DFPRVRYLMD EYENHPTQKP
210 220 230 240 250
EALLKRIILA SSNPGDIVLD PFAGSFTTGA VAIASGRKFI GIEINSEYIK
260 270 280 290
MGLRRLDVAS HYSAEELAKV KKRKTGNLSK RSRLSEVDPD LITK
Length:294
Mass (Da):33,397
Last modified:December 15, 1998 - v3
Checksum:i6BCC49D9A571884B
GO

Sequence cautioni

The sequence AAA58066.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281K → N in AAA23784. (PubMed:1459953)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58066.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76294.2.
AP009048 Genomic DNA. Translation: BAE77303.1.
M95784 Genomic DNA. Translation: AAA23784.1.
J03245 Genomic DNA. Translation: AAA83857.1.
PIRiH65118.
RefSeqiNP_417728.4. NC_000913.3.
YP_491444.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC76294; AAC76294; b3262.
BAE77303; BAE77303; BAE77303.
GeneIDi12933456.
947695.
KEGGiecj:Y75_p3180.
eco:b3262.
PATRICi32121954. VBIEscCol129921_3361.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18997 Genomic DNA. Translation: AAA58066.1 . Different initiation.
U00096 Genomic DNA. Translation: AAC76294.2 .
AP009048 Genomic DNA. Translation: BAE77303.1 .
M95784 Genomic DNA. Translation: AAA23784.1 .
J03245 Genomic DNA. Translation: AAA83857.1 .
PIRi H65118.
RefSeqi NP_417728.4. NC_000913.3.
YP_491444.1. NC_007779.1.

3D structure databases

ProteinModelPortali P28638.
SMRi P28638. Positions 26-249.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-12296N.
IntActi P28638. 34 interactions.
STRINGi 511145.b3262.

Protein family/group databases

REBASEi 3885. M.EcoKII.

Proteomic databases

PRIDEi P28638.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC76294 ; AAC76294 ; b3262 .
BAE77303 ; BAE77303 ; BAE77303 .
GeneIDi 12933456.
947695.
KEGGi ecj:Y75_p3180.
eco:b3262.
PATRICi 32121954. VBIEscCol129921_3361.

Organism-specific databases

EchoBASEi EB1461.
EcoGenei EG11498. yhdJ.

Phylogenomic databases

eggNOGi COG0863.
HOGENOMi HOG000125330.
InParanoidi P28638.
KOi K07319.
OMAi RQETRMV.
OrthoDBi EOG6FV84T.
PhylomeDBi P28638.

Enzyme and pathway databases

BioCyci EcoCyc:EG11498-MONOMER.
ECOL316407:JW5543-MONOMER.
MetaCyc:EG11498-MONOMER.

Miscellaneous databases

PROi P28638.

Gene expression databases

Genevestigatori P28638.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR002941. DNA_methylase_N4/N6.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR001091. RM_Methylase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF01555. N6_N4_Mtase. 1 hit.
[Graphical view ]
PRINTSi PR00508. S21N4MTFRASE.
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli."
    Ball C.A., Osuna R., Ferguson K.C., Johnson R.C.
    J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
  4. "Isolation of the gene encoding the Hin recombinational enhancer binding protein."
    Johnson R.C., Ball C.A., Pfeffer D., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Strain: K12.
  5. "YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica."
    Broadbent S.E., Balbontin R., Casadesus J., Marinus M.G., van der Woude M.
    J. Bacteriol. 189:4325-4327(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiYHDJ_ECOLI
AccessioniPrimary (citable) accession number: P28638
Secondary accession number(s): Q2M8V3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3