P28638 (YHDJ_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA adenine methyltransferase YhdJ
EC=2.1.1.72

Gene names

Name:	yhdJ
Ordered Locus Names:	b3262, JW5543

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Methylates the second adenine of the NsiI recognition sequence (5'-ATGCAT-3'). Ref.5
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine. Ref.5
Disruption phenotype	Mutants are viable and show no significant growth deficiency and no alteration of cell morphology. Ref.5
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.
Sequence caution	The sequence AAA58066.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro N-methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from direct assay Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	DNA adenine methyltransferase YhdJ		PRO_0000088000
Experimental info
	Sequence conflict	28	1	K → N in AAA23784. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P28638 [UniParc]. Last modified December 15, 1998. Version 3. Checksum: 6BCC49D9A571884B	FASTA	294	33,397
	10 20 30 40 50 60 MRTGCEPTRF GNEAKTIIHG DALAELKKIP AESVDLIFAD PPYNIGKNFD GLIEAWKEDL 70 80 90 100 110 120 FIDWLFEVIA ECHRVLKKQG SMYIMNSTEN MPFIDLQCRK LFTIKSRIVW SYDSSGVQAK 130 140 150 160 170 180 KHYGSMYEPI LMMVKDAKNY TFNGDAILVE AKTGSQRALI DYRKNPPQPY NHQKVPGNVW 190 200 210 220 230 240 DFPRVRYLMD EYENHPTQKP EALLKRIILA SSNPGDIVLD PFAGSFTTGA VAIASGRKFI 250 260 270 280 290 GIEINSEYIK MGLRRLDVAS HYSAEELAKV KKRKTGNLSK RSRLSEVDPD LITK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[2]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	"Dramatic changes in Fis levels upon nutrient upshift in Escherichia coli." Ball C.A., Osuna R., Ferguson K.C., Johnson R.C. J. Bacteriol. 174:8043-8056(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-100.
[4]	"Isolation of the gene encoding the Hin recombinational enhancer binding protein." Johnson R.C., Ball C.A., Pfeffer D., Simon M.I. Proc. Natl. Acad. Sci. U.S.A. 85:3484-3488(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: K12.
[5]	"YhdJ, a nonessential CcrM-like DNA methyltransferase of Escherichia coli and Salmonella enterica." Broadbent S.E., Balbontin R., Casadesus J., Marinus M.G., van der Woude M. J. Bacteriol. 189:4325-4327(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18997 Genomic DNA. Translation: AAA58066.1. Different initiation. U00096 Genomic DNA. Translation: AAC76294.2. AP009048 Genomic DNA. Translation: BAE77303.1. M95784 Genomic DNA. Translation: AAA23784.1. J03245 Genomic DNA. Translation: AAA83857.1.
PIR	H65118.
RefSeq	NP_417728.4. NC_000913.2. YP_491444.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P28638.
SMR	P28638. Positions 26-249.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-12296N.
IntAct	P28638. 1 interaction.
STRING	511145.b3262.
Protein family/group databases
REBASE	3885. M.EcoKII.
Proteomic databases
PRIDE	P28638.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC76294; AAC76294; b3262. BAE77303; BAE77303; BAE77303.
GeneID	12933456. 947695.
KEGG	ecj:Y75_p3180. eco:b3262.
PATRIC	32121954. VBIEscCol129921_3361.
Organism-specific databases
EchoBASE	EB1461.
EcoGene	EG11498. yhdJ.
Phylogenomic databases
eggNOG	COG0863.
HOGENOM	HOG000125330.
KO	K07319.
OMA	KVHSTQK.
ProtClustDB	PRK11524.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11498-MONOMER. ECOL316407:JW5543-MONOMER. MetaCyc:EG11498-MONOMER.
Gene expression databases
Genevestigator	P28638.
Family and domain databases
InterPro	IPR002941. DNA_methylase_N4/N6. IPR002052. DNA_methylase_N6_adenine_CS. IPR001091. RM_Methylase. [Graphical view]
Pfam	PF01555. N6_N4_Mtase. 1 hit. [Graphical view]
PRINTS	PR00508. S21N4MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

YHDJ_ECOLI

Accession

Primary (citable) accession number: P28638
Secondary accession number(s): Q2M8V3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 15, 1998
Last modified:	May 1, 2013
This is version 108 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program