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Protein

D-methionine-binding lipoprotein MetQ

Gene

metQ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This protein is a component of a D-methionine permease, a binding protein-dependent, ATP-driven transport system.1 Publication

GO - Biological processi

  • D-methionine transport Source: CACAO
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:METQ-MONOMER.
ECOL316407:JW0193-MONOMER.
MetaCyc:METQ-MONOMER.
RETL1328306-WGS:GSTH-7000-MONOMER.

Protein family/group databases

TCDBi3.A.1.24.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
D-methionine-binding lipoprotein MetQ
Gene namesi
Name:metQ
Synonyms:yaeC
Ordered Locus Names:b0197, JW0193
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11504. metQ.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222PROSITE-ProRule annotationAdd
BLAST
Chaini23 – 271249D-methionine-binding lipoprotein MetQPRO_0000019739Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi23 – 231N-palmitoyl cysteinePROSITE-ProRule annotation
Lipidationi23 – 231S-diacylglycerol cysteinePROSITE-ProRule annotation

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

EPDiP28635.
PaxDbiP28635.
PRIDEiP28635.

2D gel databases

SWISS-2DPAGEP28635.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dnaKP0A6Y81EBI-1114713,EBI-542092

Protein-protein interaction databases

BioGridi4263377. 8 interactions.
DIPiDIP-11196N.
IntActiP28635. 3 interactions.
STRINGi511145.b0197.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi32 – 387Combined sources
Helixi41 – 5717Combined sources
Beta strandi60 – 7011Combined sources
Helixi71 – 777Combined sources
Beta strandi80 – 889Combined sources
Helixi89 – 9911Combined sources
Beta strandi103 – 1086Combined sources
Beta strandi115 – 1173Combined sources
Helixi124 – 1263Combined sources
Beta strandi132 – 1365Combined sources
Helixi139 – 15113Combined sources
Helixi167 – 1693Combined sources
Beta strandi170 – 1723Combined sources
Beta strandi178 – 1825Combined sources
Helixi184 – 1863Combined sources
Helixi187 – 1904Combined sources
Beta strandi196 – 2016Combined sources
Helixi203 – 2064Combined sources
Helixi207 – 2093Combined sources
Helixi213 – 2164Combined sources
Beta strandi218 – 2203Combined sources
Beta strandi229 – 2346Combined sources
Helixi235 – 2373Combined sources
Helixi241 – 25010Combined sources
Helixi253 – 26210Combined sources
Turni263 – 2653Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YAHX-ray1.60X1-271[»]
ProteinModelPortaliP28635.
SMRiP28635. Positions 29-271.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NlpA lipoprotein family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D01. Bacteria.
COG1464. LUCA.
HOGENOMiHOG000222926.
InParanoidiP28635.
KOiK02073.
OMAiKAGNENS.
OrthoDBiEOG6P073X.
PhylomeDBiP28635.

Family and domain databases

InterProiIPR004872. Lipoprotein_NlpA.
[Graphical view]
PANTHERiPTHR30429. PTHR30429. 1 hit.
PfamiPF03180. Lipoprotein_9. 1 hit.
[Graphical view]
PIRSFiPIRSF002854. MetQ. 1 hit.
TIGRFAMsiTIGR00363. TIGR00363. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28635-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFKFKTFAA VGALIGSLAL VGCGQDEKDP NHIKVGVIVG AEQQVAEVAQ
60 70 80 90 100
KVAKDKYGLD VELVTFNDYV LPNEALSKGD IDANAFQHKP YLDQQLKDRG
110 120 130 140 150
YKLVAVGNTF VYPIAGYSKK IKSLDELQDG SQVAVPNDPT NLGRSLLLLQ
160 170 180 190 200
KVGLIKLKDG VGLLPTVLDV VENPKNLKIV ELEAPQLPRS LDDAQIALAV
210 220 230 240 250
INTTYASQIG LTPAKDGIFV EDKESPYVNL IVTREDNKDA ENVKKFVQAY
260 270
QSDEVYEAAN KVFNGGAVKG W
Length:271
Mass (Da):29,432
Last modified:July 1, 1993 - v2
Checksum:iB50CBC6FB5CD2BF7
GO

Sequence cautioni

The sequence AAA24507.1 differs from that shown. Reason: Frameshift at position 217. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti200 – 2001V → L in AAA24507 (PubMed:1459951).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA. Translation: BAA03657.1.
U70214 Genomic DNA. Translation: AAB08625.1.
U00096 Genomic DNA. Translation: AAC73308.1.
AP009048 Genomic DNA. Translation: BAA77874.1.
L04474 Genomic DNA. Translation: AAA24507.1. Frameshift.
PIRiE64744.
RefSeqiNP_414739.1. NC_000913.3.
WP_000874226.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73308; AAC73308; b0197.
BAA77874; BAA77874; BAA77874.
GeneIDi944893.
KEGGiecj:JW0193.
eco:b0197.
PATRICi32115507. VBIEscCol129921_0205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA. Translation: BAA03657.1.
U70214 Genomic DNA. Translation: AAB08625.1.
U00096 Genomic DNA. Translation: AAC73308.1.
AP009048 Genomic DNA. Translation: BAA77874.1.
L04474 Genomic DNA. Translation: AAA24507.1. Frameshift.
PIRiE64744.
RefSeqiNP_414739.1. NC_000913.3.
WP_000874226.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4YAHX-ray1.60X1-271[»]
ProteinModelPortaliP28635.
SMRiP28635. Positions 29-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263377. 8 interactions.
DIPiDIP-11196N.
IntActiP28635. 3 interactions.
STRINGi511145.b0197.

Protein family/group databases

TCDBi3.A.1.24.1. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP28635.

Proteomic databases

EPDiP28635.
PaxDbiP28635.
PRIDEiP28635.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73308; AAC73308; b0197.
BAA77874; BAA77874; BAA77874.
GeneIDi944893.
KEGGiecj:JW0193.
eco:b0197.
PATRICi32115507. VBIEscCol129921_0205.

Organism-specific databases

EchoBASEiEB1467.
EcoGeneiEG11504. metQ.

Phylogenomic databases

eggNOGiENOG4105D01. Bacteria.
COG1464. LUCA.
HOGENOMiHOG000222926.
InParanoidiP28635.
KOiK02073.
OMAiKAGNENS.
OrthoDBiEOG6P073X.
PhylomeDBiP28635.

Enzyme and pathway databases

BioCyciEcoCyc:METQ-MONOMER.
ECOL316407:JW0193-MONOMER.
MetaCyc:METQ-MONOMER.
RETL1328306-WGS:GSTH-7000-MONOMER.

Miscellaneous databases

PROiP28635.

Family and domain databases

InterProiIPR004872. Lipoprotein_NlpA.
[Graphical view]
PANTHERiPTHR30429. PTHR30429. 1 hit.
PfamiPF03180. Lipoprotein_9. 1 hit.
[Graphical view]
PIRSFiPIRSF002854. MetQ. 1 hit.
TIGRFAMsiTIGR00363. TIGR00363. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of 5' flanking region of the ribosomal RNA gene (rrnH) in E. coli."
    Miyamoto K.
    Submitted (APR-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Systematic sequencing of the Escherichia coli genome: analysis of the 4.0 - 6.0 min (189,987 - 281,416bp) region."
    Takemoto K., Mori H., Murayama N., Kataoka K., Yano M., Itoh T., Yamamoto Y., Inokuchi H., Miki T., Hatada E., Fukuda R., Ichihara S., Mizuno T., Makino K., Nakata A., Yura T., Sampei G., Mizobuchi K.
    Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Identification, cloning, and characterization of rcsF, a new regulator gene for exopolysaccharide synthesis that suppresses the division mutation ftsZ84 in Escherichia coli K-12."
    Gervais F.G., Drapeau G.R.
    J. Bacteriol. 174:8016-8022(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 127-271.
    Strain: K12.
  7. "The metD D-methionine transporter locus of Escherichia coli is an ABC transporter gene cluster."
    Gal J., Szvetnik A., Schnell R., Kalman M.
    J. Bacteriol. 184:4930-4932(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMETQ_ECOLI
AccessioniPrimary (citable) accession number: P28635
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: July 1, 1993
Last modified: March 16, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The MetNIQ system is also to be able to transport the toxic methionine analog alpha-methyl-methionine.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.