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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

trmO

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Thr)(GGU) that read codons starting with adenosine (PubMed:9537379, PubMed:25063302). The methyl group of m6t6A37 appears to slightly improve the efficiency of the tRNA decoding ability (PubMed:9537379, PubMed:25063302). Binds to tRNA (PubMed:25063302).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92S-adenosyl-L-methionineBy similarity1

GO - Molecular functioni

  • tRNA binding Source: UniProtKB
  • tRNA m6t6A37 methyltransferase activity Source: EcoCyc

GO - Biological processi

  • tRNA methylation Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG11503-MONOMER
MetaCyc:EG11503-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferaseCurated (EC:2.1.1.-1 Publication)
Alternative name(s):
tRNA (m6t6A37) methyltransferase1 Publication
tRNA methyltransferase O1 Publication
Gene namesi
Name:trmO1 Publication
Synonyms:tsaA1 Publication, yaeB
Ordered Locus Names:b0195, JW0191
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11503 tsaA

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92R → A: Inhibits tRNA m(6)t(6)A37 formation. 1 Publication1
Mutagenesisi136K → A: Inhibits tRNA m(6)t(6)A37 formation. 1 Publication1
Mutagenesisi194D → A: Inhibits tRNA m(6)t(6)A37 formation; when associated with A-196. 1 Publication1
Mutagenesisi196R → A: Inhibits tRNA m(6)t(6)A37 formation; when associated with A-194. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001556171 – 235tRNA (adenine(37)-N6)-methyltransferaseAdd BLAST235

Proteomic databases

PaxDbiP28634
PRIDEiP28634

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi4259756, 15 interactors
DIPiDIP-11195N
IntActiP28634, 6 interactors
STRINGi316385.ECDH10B_0176

Structurei

3D structure databases

ProteinModelPortaliP28634
SMRiP28634
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 147TsaA-likePROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 25S-adenosyl-L-methionine bindingBy similarity3
Regioni64 – 65S-adenosyl-L-methionine bindingBy similarity2
Regioni127 – 130S-adenosyl-L-methionine bindingBy similarity4

Phylogenomic databases

eggNOGiENOG4108R96 Bacteria
COG1720 LUCA
HOGENOMiHOG000225518
InParanoidiP28634
OMAiCFKEKFA
PhylomeDBiP28634

Family and domain databases

CDDicd09281 UPF0066, 1 hit
Gene3Di2.40.30.70, 1 hit
InterProiView protein in InterPro
IPR023368 UPF0066_cons_site
IPR023370 YaeB-like_N
IPR036413 YaeB-like_sf
IPR036414 YaeB_N_sf
PfamiView protein in Pfam
PF01980 UPF0066, 1 hit
SUPFAMiSSF118196 SSF118196, 1 hit
TIGRFAMsiTIGR00104 tRNA_TsaA, 1 hit
PROSITEiView protein in PROSITE
PS01318 TSAA_1, 1 hit
PS51668 TSAA_2, 1 hit

Sequencei

Sequence statusi: Complete.

P28634-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSFQFEQIG VIRSPYKEKF AVPRQPGLVK SANGELHLIA PYNQADAVRG
60 70 80 90 100
LEAFSHLWIL FVFHQTMEGG WRPTVRPPRL GGNARMGVFA TRSTFRPNPI
110 120 130 140 150
GMSLVELKEV VCHKDSVILK LGSLDLVDGT PVVDIKPYLP FAESLPDASA
160 170 180 190 200
SYAQSAPAAE MAVSFTAEVE KQLLTLEKRY PQLTLFIREV LAQDPRPAYR
210 220 230
KGEETGKTYA VWLHDFNVRW RVTDAGFEVF ALEPR
Length:235
Mass (Da):26,362
Last modified:October 1, 1994 - v2
Checksum:iB19F8D50DECCB572
GO

Sequence cautioni

The sequence AAA24509 differs from that shown. Reason: Frameshift at several positions.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D15061 Genomic DNA Translation: BAA03655.1
L04474 Genomic DNA Translation: AAA24509.1 Frameshift.
U70214 Genomic DNA Translation: AAB08623.1
U00096 Genomic DNA Translation: AAC73306.1
AP009048 Genomic DNA Translation: BAA77871.1
PIRiC47040
C64744
RefSeqiNP_414737.1, NC_000913.3
WP_000094011.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73306; AAC73306; b0195
BAA77871; BAA77871; BAA77871
GeneIDi949112
KEGGiecj:JW0191
eco:b0195
PATRICifig|1411691.4.peg.2083

Similar proteinsi

Entry informationi

Entry nameiTRMO_ECOLI
AccessioniPrimary (citable) accession number: P28634
Secondary accession number(s): Q47676
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: October 1, 1994
Last modified: March 28, 2018
This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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