Reviewed, UniProtKB/Swiss-Prot P28629 (ADIA_ECOLI)

Names and origin

Protein names

Recommended name:
    Biodegradative arginine decarboxylase
      Short name=ADC
    EC=4.1.1.19

Gene names

Name:	adiA
Synonyms:	adi
Ordered Locus Names:	b4117, JW5731

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	755 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ADC can be found in two forms: biodegradative and biosynthetic. The biodegradative form may play a role in regulating pH by consuming proteins.
Catalytic activity	L-arginine = agmatine + CO2.
Cofactor	Pyridoxal phosphate.
Subunit structure	Homodecamer.
Subcellular location	Cytoplasm.
Induction	Under conditions of acidic pH, anaerobiosis and rich medium.
Sequence similarities	Belongs to the Orn/Lys/Arg decarboxylase class-I family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB
Molecular function	arginine decarboxylase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 755	755	Biodegradative arginine decarboxylase		PRO_0000201149
Amino acid modifications
	Modified residue	386	1	N6-(pyridoxal phosphate)lysine
Experimental info
	Sequence conflict	75	1	L → P Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P28629-1 [UniParc]. Last modified December 1, 1992. Version 1. Checksum: 0FCB715144649F8F	FASTA	755	84,425
	10 20 30 40 50 60 MKVLIVESEF LHQDTWVGNA VERLADALSQ QNVTVIKSTS FDDGFAILSS NEAIDCLMFS 70 80 90 100 110 120 YQMEHPDEHQ NVRQLIGKLH ERQQNVPVFL LGDREKALAA MDRDLLELVD EFAWILEDTA 130 140 150 160 170 180 DFIAGRAVAA MTRYRQQLLP PLFSALMKYS DIHEYSWAAP GHQGGVGFTK TPAGRFYHDY 190 200 210 220 230 240 YGENLFRTDM GIERTSLGSL LDHTGAFGES EKYAARVFGA DRSWSVVVGT SGSNRTIMQA 250 260 270 280 290 300 CMTDNDVVVV DRNCHKSIEQ GLMLTGAKPV YMVPSRNRYG IIGPIYPQEM QPETLQKKIS 310 320 330 340 350 360 ESPLTKDKAG QKPSYCVVTN CTYDGVCYNA KEAQDLLEKT SDRLHFDEAW YGYARFNPIY 370 380 390 400 410 420 ADHYAMRGEP GDHNGPTVFA THSTHKLLNA LSQASYIHVR EGRGAINFSR FNQAYMMHAT 430 440 450 460 470 480 TSPLYAICAS NDVAVSMMDG NSGLSLTQEV IDEAVDFRQA MARLYKEFTA DGSWFFKPWN 490 500 510 520 530 540 KEVVTDPQTG KTYDFADAPT KLLTTVQDCW VMHPGESWHG FKDIPDNWSM LDPIKVSILA 550 560 570 580 590 600 PGMGEDGELE ETGVPAALVT AWLGRHGIVP TRTTDFQIMF LFSMGVTRGK WGTLVNTLCS 610 620 630 640 650 660 FKRHYDANTP LAQVMPELVE QYPDTYANMG IHDLGDTMFA WLKENNPGAR LNEAYSGLPV 670 680 690 700 710 720 AEVTPREAYN AIVDNNVELV SIENLPGRIA ANSVIPYPPG IPMLLSGENF GDKNSPQVSY 730 740 750 LRSLQSWDHH FPGFEHETEG TEIIDGIYHV MCVKA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the adi gene, which encodes the biodegradative acid-induced arginine decarboxylase of Escherichia coli." Stim K.P., Bennett G.N. J. Bacteriol. 175:1221-1234(1993) [PubMed: 8383109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R. Nucleic Acids Res. 23:2105-2119(1995) [PubMed: 7610040] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Organisation of the regulatory region of the Escherichia coli melibiose operon." Webster C., Kempsell K., Booth I., Busby S. Gene 59:253-263(1987) [PubMed: 2830169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-78.
[6]	"Chemical properties of Escherichia coli lysine decarboxylase including a segment of its pyridoxal 5'-phosphate binding site." Sabo D.L., Fischer E.H. Biochemistry 13:670-676(1974) [PubMed: 4204273] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-7. Strain: B.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M93362 Genomic DNA. Translation: AAA23481.1.
U14003 Genomic DNA. Translation: AAA97017.1. Different initiation.
U00096 Genomic DNA. Translation: AAC77078.2.
AP009048 Genomic DNA. Translation: BAE78119.1. Different initiation.
M18425 Genomic DNA. No translation available.

PIR

S56346.

RefSeq

AP_004618.1.
NP_418541.2.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VYC	X-ray	2.40	A/B/C/D/E/F/G/H/I/J	1-755	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP:2903N.

Genome annotation databases

GeneID

948638.

GenomeReviews

Gene locus JW5731 in contig AP009048_GR.
Gene locus b4117 in contig U00096_GR.

KEGG

ecj:JW5731.
eco:b4117.

Organism-specific databases

EchoBASE

EB1464.

EcoGene

EG11501. adiA.

CMR

Search...

Phylogenomic databases

HOGENOM

P28629.

OMA

P28629. ANDWVEC.

Enzyme and pathway databases

BioCyc

EcoCyc:ARGDECARBOXDEG-MON.
MetaCyc:ARGDECARBOXDEG-MON.

Family and domain databases

InterPro

IPR000310. Decarbxylse1.
IPR005308. OKR_de-COase_N.
IPR011193. Orn/lys/arg_de-COase.
IPR008286. Prn/Lys/Arg_de-COase_C.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]

Gene3D

G3DSA:3.90.100.10. Decarbxylse_C. 1 hit.
G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.

Pfam

PF01276. OKR_DC_1. 1 hit.
PF03711. OKR_DC_1_C. 1 hit.
PF03709. OKR_DC_1_N. 1 hit.
[Graphical view]

PIRSF

PIRSF009393. Orn_decarb. 1 hit.

PROSITE

PS00703. OKR_DC_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ADIA_ECOLI

Accession

Primary (citable) accession number: P28629
Secondary accession number(s): P78138, Q2M6I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 1992
Last sequence update:	December 1, 1992
Last modified:	June 16, 2009
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)