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Protein

Signal peptidase I S

Gene

sipS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Not essential for cell viability, but required for efficient secretion of many proteins.1 Publication

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431
Active sitei83 – 831

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciBSUB:BSU23310-MONOMER.
BRENDAi3.4.21.89. 658.

Protein family/group databases

MEROPSiS26.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase I S (EC:3.4.21.89)
Short name:
SPase I
Alternative name(s):
Leader peptidase I
Gene namesi
Name:sipS
Ordered Locus Names:BSU23310
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1818CytoplasmicSequence analysisAdd
BLAST
Transmembranei19 – 3921HelicalSequence analysisAdd
BLAST
Topological domaini40 – 184145ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi42 – 421D → S: No effect. 1 Publication
Mutagenesisi43 – 431S → A or V: Loss of activity. 1 Publication
Mutagenesisi43 – 431S → C or T: Reduced activity. 1 Publication
Mutagenesisi44 – 441M → A: Increased activity. 1 Publication
Mutagenesisi46 – 461P → A: Slightly reduced activity. 1 Publication
Mutagenesisi47 – 471T → A: No effect. 1 Publication
Mutagenesisi48 – 481L → A: Reduced activity. 1 Publication
Mutagenesisi69 – 691G → A: Slightly reduced activity. 1 Publication
Mutagenesisi70 – 701D → A: Slightly reduced activity. 1 Publication
Mutagenesisi71 – 711I → A: Slightly reduced activity. 1 Publication
Mutagenesisi72 – 721V → A: No effect. 1 Publication
Mutagenesisi74 – 741L → A: Reduced activity. 1 Publication
Mutagenesisi79 – 791V → A: No effect. 1 Publication
Mutagenesisi81 – 811Y → A: Reduced activity. 1 Publication
Mutagenesisi81 – 811Y → F: No effect. 1 Publication
Mutagenesisi83 – 831K → A, H or R: Loss of activity. 1 Publication
Mutagenesisi84 – 841R → A: Loss of activity. 1 Publication
Mutagenesisi84 – 841R → H: Strongly reduced activity. 1 Publication
Mutagenesisi84 – 841R → K: No effect. 1 Publication
Mutagenesisi86 – 861I → A: Slightly reduced activity. 1 Publication
Mutagenesisi87 – 871G → A: No effect. 1 Publication
Mutagenesisi88 – 881L → A: Reduced activity. 1 Publication
Mutagenesisi89 – 891P → A: No effect. 1 Publication
Mutagenesisi90 – 901G → A: No effect. 1 Publication
Mutagenesisi91 – 911D → A, E or N: No effect. 1 Publication
Mutagenesisi141 – 1411Y → A: No effect. 1 Publication
Mutagenesisi145 – 1451G → A: Strongly reduced activity. 1 Publication
Mutagenesisi146 – 1461D → A or N: Strongly reduced activity. 1 Publication
Mutagenesisi146 – 1461D → E: No effect. 1 Publication
Mutagenesisi147 – 1471N → A: Reduced activity. 1 Publication
Mutagenesisi150 – 1501N → A: No effect. 1 Publication
Mutagenesisi151 – 1511S → A: Reduced activity. 1 Publication
Mutagenesisi153 – 1531D → A: Strongly reduced activity. 1 Publication
Mutagenesisi153 – 1531D → E or N: Loss of activity. 1 Publication
Mutagenesisi154 – 1541S → A: Slightly reduced activity. 1 Publication
Mutagenesisi155 – 1551R → A: Reduced activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 184184Signal peptidase I SPRO_0000109499Add
BLAST

Proteomic databases

PaxDbiP28628.

Expressioni

Inductioni

Expressed at the postexponential growth phase; regulated by the DegS-DegU system.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012791.

Structurei

3D structure databases

ProteinModelPortaliP28628.
SMRiP28628. Positions 35-86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003673.
InParanoidiP28628.
KOiK03100.
OMAiMVPLDRV.
PhylomeDBiP28628.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSENVSKKK SILEWAKAIV IAVVLALLIR NFIFAPYVVD GDSMYPTLHN
60 70 80 90 100
RERVFVNMTV KYIGEFDRGD IVVLNGDDVH YVKRIIGLPG DTVEMKNDQL
110 120 130 140 150
YINGKKVDEP YLAANKKRAK QDGFDHLTDD FGPVKVPDNK YFVMGDNRRN
160 170 180
SMDSRNGLGL FTKKQIAGTS KFVFYPFNEM RKTN
Length:184
Mass (Da):21,047
Last modified:December 1, 1992 - v1
Checksum:i5A2D005BF0D33CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11847 Genomic DNA. Translation: CAA77871.1.
L09228 Genomic DNA. Translation: AAA67478.1.
AL009126 Genomic DNA. Translation: CAB14263.1.
PIRiS23381.
RefSeqiNP_390212.1. NC_000964.3.
WP_003246166.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14263; CAB14263; BSU23310.
GeneIDi938944.
KEGGibsu:BSU23310.
PATRICi18976477. VBIBacSub10457_2429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11847 Genomic DNA. Translation: CAA77871.1.
L09228 Genomic DNA. Translation: AAA67478.1.
AL009126 Genomic DNA. Translation: CAB14263.1.
PIRiS23381.
RefSeqiNP_390212.1. NC_000964.3.
WP_003246166.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP28628.
SMRiP28628. Positions 35-86.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012791.

Protein family/group databases

MEROPSiS26.003.

Proteomic databases

PaxDbiP28628.

Protocols and materials databases

DNASUi938944.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14263; CAB14263; BSU23310.
GeneIDi938944.
KEGGibsu:BSU23310.
PATRICi18976477. VBIBacSub10457_2429.

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003673.
InParanoidiP28628.
KOiK03100.
OMAiMVPLDRV.
PhylomeDBiP28628.

Enzyme and pathway databases

BioCyciBSUB:BSU23310-MONOMER.
BRENDAi3.4.21.89. 658.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPS_BACSU
AccessioniPrimary (citable) accession number: P28628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.