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Protein

Signal peptidase I S

Gene

sipS

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Not essential for cell viability, but required for efficient secretion of many proteins.1 Publication

Catalytic activityi

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei431
Active sitei831

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciBSUB:BSU23310-MONOMER.
BRENDAi3.4.21.89. 658.

Protein family/group databases

MEROPSiS26.003.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal peptidase I S (EC:3.4.21.89)
Short name:
SPase I
Alternative name(s):
Leader peptidase I
Gene namesi
Name:sipS
Ordered Locus Names:BSU23310
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 39HelicalSequence analysisAdd BLAST21
Topological domaini40 – 184ExtracellularSequence analysisAdd BLAST145

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi42D → S: No effect. 1 Publication1
Mutagenesisi43S → A or V: Loss of activity. 1 Publication1
Mutagenesisi43S → C or T: Reduced activity. 1 Publication1
Mutagenesisi44M → A: Increased activity. 1 Publication1
Mutagenesisi46P → A: Slightly reduced activity. 1 Publication1
Mutagenesisi47T → A: No effect. 1 Publication1
Mutagenesisi48L → A: Reduced activity. 1 Publication1
Mutagenesisi69G → A: Slightly reduced activity. 1 Publication1
Mutagenesisi70D → A: Slightly reduced activity. 1 Publication1
Mutagenesisi71I → A: Slightly reduced activity. 1 Publication1
Mutagenesisi72V → A: No effect. 1 Publication1
Mutagenesisi74L → A: Reduced activity. 1 Publication1
Mutagenesisi79V → A: No effect. 1 Publication1
Mutagenesisi81Y → A: Reduced activity. 1 Publication1
Mutagenesisi81Y → F: No effect. 1 Publication1
Mutagenesisi83K → A, H or R: Loss of activity. 1 Publication1
Mutagenesisi84R → A: Loss of activity. 1 Publication1
Mutagenesisi84R → H: Strongly reduced activity. 1 Publication1
Mutagenesisi84R → K: No effect. 1 Publication1
Mutagenesisi86I → A: Slightly reduced activity. 1 Publication1
Mutagenesisi87G → A: No effect. 1 Publication1
Mutagenesisi88L → A: Reduced activity. 1 Publication1
Mutagenesisi89P → A: No effect. 1 Publication1
Mutagenesisi90G → A: No effect. 1 Publication1
Mutagenesisi91D → A, E or N: No effect. 1 Publication1
Mutagenesisi141Y → A: No effect. 1 Publication1
Mutagenesisi145G → A: Strongly reduced activity. 1 Publication1
Mutagenesisi146D → A or N: Strongly reduced activity. 1 Publication1
Mutagenesisi146D → E: No effect. 1 Publication1
Mutagenesisi147N → A: Reduced activity. 1 Publication1
Mutagenesisi150N → A: No effect. 1 Publication1
Mutagenesisi151S → A: Reduced activity. 1 Publication1
Mutagenesisi153D → A: Strongly reduced activity. 1 Publication1
Mutagenesisi153D → E or N: Loss of activity. 1 Publication1
Mutagenesisi154S → A: Slightly reduced activity. 1 Publication1
Mutagenesisi155R → A: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001094991 – 184Signal peptidase I SAdd BLAST184

Proteomic databases

PaxDbiP28628.

Expressioni

Inductioni

Expressed at the postexponential growth phase; regulated by the DegS-DegU system.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012791.

Structurei

3D structure databases

ProteinModelPortaliP28628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003673.
InParanoidiP28628.
KOiK03100.
OMAiMVPLDRV.
PhylomeDBiP28628.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P28628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSENVSKKK SILEWAKAIV IAVVLALLIR NFIFAPYVVD GDSMYPTLHN
60 70 80 90 100
RERVFVNMTV KYIGEFDRGD IVVLNGDDVH YVKRIIGLPG DTVEMKNDQL
110 120 130 140 150
YINGKKVDEP YLAANKKRAK QDGFDHLTDD FGPVKVPDNK YFVMGDNRRN
160 170 180
SMDSRNGLGL FTKKQIAGTS KFVFYPFNEM RKTN
Length:184
Mass (Da):21,047
Last modified:December 1, 1992 - v1
Checksum:i5A2D005BF0D33CE9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11847 Genomic DNA. Translation: CAA77871.1.
L09228 Genomic DNA. Translation: AAA67478.1.
AL009126 Genomic DNA. Translation: CAB14263.1.
PIRiS23381.
RefSeqiNP_390212.1. NC_000964.3.
WP_003246166.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14263; CAB14263; BSU23310.
GeneIDi938944.
KEGGibsu:BSU23310.
PATRICi18976477. VBIBacSub10457_2429.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z11847 Genomic DNA. Translation: CAA77871.1.
L09228 Genomic DNA. Translation: AAA67478.1.
AL009126 Genomic DNA. Translation: CAB14263.1.
PIRiS23381.
RefSeqiNP_390212.1. NC_000964.3.
WP_003246166.1. NZ_JNCM01000036.1.

3D structure databases

ProteinModelPortaliP28628.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100012791.

Protein family/group databases

MEROPSiS26.003.

Proteomic databases

PaxDbiP28628.

Protocols and materials databases

DNASUi938944.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14263; CAB14263; BSU23310.
GeneIDi938944.
KEGGibsu:BSU23310.
PATRICi18976477. VBIBacSub10457_2429.

Phylogenomic databases

eggNOGiENOG4105C3F. Bacteria.
COG0681. LUCA.
HOGENOMiHOG000003673.
InParanoidiP28628.
KOiK03100.
OMAiMVPLDRV.
PhylomeDBiP28628.

Enzyme and pathway databases

BioCyciBSUB:BSU23310-MONOMER.
BRENDAi3.4.21.89. 658.

Family and domain databases

Gene3Di2.10.109.10. 2 hits.
InterProiIPR000223. Pept_S26A_signal_pept_1.
IPR019758. Pept_S26A_signal_pept_1_CS.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
PS00761. SPASE_I_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLEPS_BACSU
AccessioniPrimary (citable) accession number: P28628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: December 1, 1992
Last modified: October 5, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

B.subtilis contains five chromosomal type I signal peptidases: SipS, SipT, SipU, SipV and SipW. They have different, but overlapping, substrate specificities and have different transcription patterns.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.