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P28627

- IMP1_YEAST

UniProt

P28627 - IMP1_YEAST

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Protein

Mitochondrial inner membrane protease subunit 1

Gene

IMP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic component of the mitochondrial inner membrane peptidase (IMP) complex. IMP catalyzes the removal of signal peptides required for the targeting of proteins from the mitochondrial matrix, across the inner membrane, into the inter-membrane space. The two catalytic IMP subunits seem to have non-overlapping substrate specificities. IMP1 substrates include nuclear encoded CYB2, mitochondrially encoded COX2, NADH-cytochrome b5 reductase and GUT2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei40 – 401By similarity
Active sitei84 – 841By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: SGD
  2. serine-type peptidase activity Source: InterPro

GO - Biological processi

  1. protein processing involved in protein targeting to mitochondrion Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32841-MONOMER.

Protein family/group databases

MEROPSiS26.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial inner membrane protease subunit 1 (EC:3.4.21.-)
Gene namesi
Name:IMP1
Synonyms:PET2858
Ordered Locus Names:YMR150C
ORF Names:YM9375.20C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

SGDiS000004758. IMP1.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: InterPro
  2. mitochondrial inner membrane peptidase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi40 – 401S → A or T: Abolishes enzymatic activity. 1 Publication
Mutagenesisi84 – 841K → H or R: Abolishes enzymatic activity. 1 Publication
Mutagenesisi85 – 851R → A: Reduces enzymatic activity. 1 Publication
Mutagenesisi88 – 881G → D in pet ts2858; temperature-sensitive; abolishes processing of CYB2 and presence of SOM1 in the IMP complex. 2 Publications
Mutagenesisi130 – 1301G → S: Abolishes processing of CYB2 and COX2 and presence of SOM1 in the IMP complex. 1 Publication
Mutagenesisi131 – 1311D → E or N: Reduces enzymatic activity. 1 Publication
Mutagenesisi131 – 1311D → Y: Abolishes enzymatic activity. 1 Publication
Mutagenesisi138 – 1381D → N or E: Abolishes enzymatic activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 190189Mitochondrial inner membrane protease subunit 1PRO_0000109537Add
BLAST

Proteomic databases

PaxDbiP28627.

Expressioni

Gene expression databases

GenevestigatoriP28627.

Interactioni

Subunit structurei

Component of the mitochondrial inner membrane peptidase (IMP) complex which at least consists of IMP1, IMP2 and SOM1.1 Publication

Protein-protein interaction databases

BioGridi35326. 14 interactions.
DIPiDIP-8328N.
IntActiP28627. 2 interactions.
STRINGi4932.YMR150C.

Structurei

3D structure databases

ProteinModelPortaliP28627.
SMRiP28627. Positions 27-158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family. IMP1 subfamily.Curated

Phylogenomic databases

eggNOGiCOG0681.
GeneTreeiENSGT00550000075025.
HOGENOMiHOG000003673.
InParanoidiP28627.
KOiK09647.
OMAiKRVTGMP.
OrthoDBiEOG70PC8B.

Family and domain databases

Gene3Di2.10.109.10. 1 hit.
InterProiIPR026730. Mitochondrial_IMP1.
IPR000223. Pept_S26A_signal_pept_1.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PTHR12383:SF14. PTHR12383:SF14. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28627-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTVGTLPIWS KTFSYAIRSL CFLHIIHMYA YEFTETRGES MLPTLSATND
60 70 80 90 100
YVHVLKNFQN GRGIKMGDCI VALKPTDPNH RICKRVTGMP GDLVLVDPST
110 120 130 140 150
IVNYVGDVLV DEERFGTYIK VPEGHVWVTG DNLSHSLDSR TYNALPMGLI
160 170 180 190
MGKIVAANNF DKPFWDGSIR NIWGFKWINN TFLDVQAKSN
Length:190
Mass (Da):21,433
Last modified:January 23, 2007 - v3
Checksum:i81B044C3B57DABB3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti178 – 1781I → V in AAS56741. (PubMed:17322287)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55518 Genomic DNA. Translation: AAB19704.1.
Z47071 Genomic DNA. Translation: CAA87365.1.
AY558415 Genomic DNA. Translation: AAS56741.1.
BK006946 Genomic DNA. Translation: DAA10046.1.
PIRiS16817.
RefSeqiNP_013870.1. NM_001182652.1.

Genome annotation databases

EnsemblFungiiYMR150C; YMR150C; YMR150C.
GeneIDi855182.
KEGGisce:YMR150C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55518 Genomic DNA. Translation: AAB19704.1 .
Z47071 Genomic DNA. Translation: CAA87365.1 .
AY558415 Genomic DNA. Translation: AAS56741.1 .
BK006946 Genomic DNA. Translation: DAA10046.1 .
PIRi S16817.
RefSeqi NP_013870.1. NM_001182652.1.

3D structure databases

ProteinModelPortali P28627.
SMRi P28627. Positions 27-158.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35326. 14 interactions.
DIPi DIP-8328N.
IntActi P28627. 2 interactions.
STRINGi 4932.YMR150C.

Protein family/group databases

MEROPSi S26.002.

Proteomic databases

PaxDbi P28627.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR150C ; YMR150C ; YMR150C .
GeneIDi 855182.
KEGGi sce:YMR150C.

Organism-specific databases

SGDi S000004758. IMP1.

Phylogenomic databases

eggNOGi COG0681.
GeneTreei ENSGT00550000075025.
HOGENOMi HOG000003673.
InParanoidi P28627.
KOi K09647.
OMAi KRVTGMP.
OrthoDBi EOG70PC8B.

Enzyme and pathway databases

BioCyci YEAST:G3O-32841-MONOMER.

Miscellaneous databases

NextBioi 978638.
PROi P28627.

Gene expression databases

Genevestigatori P28627.

Family and domain databases

Gene3Di 2.10.109.10. 1 hit.
InterProi IPR026730. Mitochondrial_IMP1.
IPR000223. Pept_S26A_signal_pept_1.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view ]
PANTHERi PTHR12383. PTHR12383. 1 hit.
PTHR12383:SF14. PTHR12383:SF14. 1 hit.
Pfami PF00717. Peptidase_S24. 1 hit.
[Graphical view ]
PRINTSi PR00727. LEADERPTASE.
SUPFAMi SSF51306. SSF51306. 1 hit.
TIGRFAMsi TIGR02227. sigpep_I_bact. 1 hit.
PROSITEi PS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mitochondrial inner membrane protease 1 of Saccharomyces cerevisiae shows sequence similarity to the Escherichia coli leader peptidase."
    Behrens M., Michaelis G., Pratje E.
    Mol. Gen. Genet. 228:167-176(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-88.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. "Purified inner membrane protease I of yeast mitochondria is a heterodimer."
    Schneider A., Oppliger W., Jenoe P.
    J. Biol. Chem. 269:8635-8638(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-16, SUBUNIT.
  6. "Signal peptides having standard and nonstandard cleavage sites can be processed by Imp1p of the mitochondrial inner membrane protease."
    Chen X., Van Valkenburgh C., Fang H., Green N.
    J. Biol. Chem. 274:37750-37754(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF SER-40; LYS-84; ARG-85; ASP-131 AND ASP-138.
  7. "The mitochondrial IMP peptidase of yeast: functional analysis of domains and identification of Gut2 as a new natural substrate."
    Esser K., Jan P.S., Pratje E., Michaelis G.
    Mol. Genet. Genomics 271:616-626(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF GLY-88 AND GLY-130.

Entry informationi

Entry nameiIMP1_YEAST
AccessioniPrimary (citable) accession number: P28627
Secondary accession number(s): D6VZX2, E9P8W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 26, 2014
This is version 129 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3