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Protein

Mitochondrial inner membrane protease subunit 1

Gene

IMP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic component of the mitochondrial inner membrane peptidase (IMP) complex. IMP catalyzes the removal of signal peptides required for the targeting of proteins from the mitochondrial matrix, across the inner membrane, into the inter-membrane space. The two catalytic IMP subunits seem to have non-overlapping substrate specificities. IMP1 substrates include nuclear encoded CYB2, mitochondrially encoded COX2, NADH-cytochrome b5 reductase and GUT2.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei40By similarity1
Active sitei84By similarity1

GO - Molecular functioni

  • endopeptidase activity Source: SGD
  • serine-type endopeptidase activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Enzyme and pathway databases

BioCyciYEAST:G3O-32841-MONOMER.

Protein family/group databases

MEROPSiS26.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial inner membrane protease subunit 1 (EC:3.4.21.-)
Gene namesi
Name:IMP1
Synonyms:PET2858
Ordered Locus Names:YMR150C
ORF Names:YM9375.20C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR150C.
SGDiS000004758. IMP1.

Subcellular locationi

GO - Cellular componenti

  • integral component of membrane Source: InterPro
  • mitochondrial inner membrane peptidase complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi40S → A or T: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi84K → H or R: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi85R → A: Reduces enzymatic activity. 1 Publication1
Mutagenesisi88G → D in pet ts2858; temperature-sensitive; abolishes processing of CYB2 and presence of SOM1 in the IMP complex. 2 Publications1
Mutagenesisi130G → S: Abolishes processing of CYB2 and COX2 and presence of SOM1 in the IMP complex. 1 Publication1
Mutagenesisi131D → E or N: Reduces enzymatic activity. 1 Publication1
Mutagenesisi131D → Y: Abolishes enzymatic activity. 1 Publication1
Mutagenesisi138D → N or E: Abolishes enzymatic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001095372 – 190Mitochondrial inner membrane protease subunit 1Add BLAST189

Proteomic databases

PRIDEiP28627.

Interactioni

Subunit structurei

Component of the mitochondrial inner membrane peptidase (IMP) complex which at least consists of IMP1, IMP2 and SOM1.1 Publication

Protein-protein interaction databases

BioGridi35326. 14 interactors.
DIPiDIP-8328N.
IntActiP28627. 2 interactors.

Structurei

3D structure databases

ProteinModelPortaliP28627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S26 family. IMP1 subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000075025.
HOGENOMiHOG000003673.
InParanoidiP28627.
KOiK09647.
OMAiPTHRICK.
OrthoDBiEOG092C52AJ.

Family and domain databases

Gene3Di2.10.109.10. 1 hit.
InterProiIPR026730. Mitochondrial_IMP1.
IPR000223. Pept_S26A_signal_pept_1.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PTHR12383:SF16. PTHR12383:SF16. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P28627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVGTLPIWS KTFSYAIRSL CFLHIIHMYA YEFTETRGES MLPTLSATND
60 70 80 90 100
YVHVLKNFQN GRGIKMGDCI VALKPTDPNH RICKRVTGMP GDLVLVDPST
110 120 130 140 150
IVNYVGDVLV DEERFGTYIK VPEGHVWVTG DNLSHSLDSR TYNALPMGLI
160 170 180 190
MGKIVAANNF DKPFWDGSIR NIWGFKWINN TFLDVQAKSN
Length:190
Mass (Da):21,433
Last modified:January 23, 2007 - v3
Checksum:i81B044C3B57DABB3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti178I → V in AAS56741 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55518 Genomic DNA. Translation: AAB19704.1.
Z47071 Genomic DNA. Translation: CAA87365.1.
AY558415 Genomic DNA. Translation: AAS56741.1.
BK006946 Genomic DNA. Translation: DAA10046.1.
PIRiS16817.
RefSeqiNP_013870.1. NM_001182652.1.

Genome annotation databases

EnsemblFungiiYMR150C; YMR150C; YMR150C.
GeneIDi855182.
KEGGisce:YMR150C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S55518 Genomic DNA. Translation: AAB19704.1.
Z47071 Genomic DNA. Translation: CAA87365.1.
AY558415 Genomic DNA. Translation: AAS56741.1.
BK006946 Genomic DNA. Translation: DAA10046.1.
PIRiS16817.
RefSeqiNP_013870.1. NM_001182652.1.

3D structure databases

ProteinModelPortaliP28627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35326. 14 interactors.
DIPiDIP-8328N.
IntActiP28627. 2 interactors.

Protein family/group databases

MEROPSiS26.002.

Proteomic databases

PRIDEiP28627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR150C; YMR150C; YMR150C.
GeneIDi855182.
KEGGisce:YMR150C.

Organism-specific databases

EuPathDBiFungiDB:YMR150C.
SGDiS000004758. IMP1.

Phylogenomic databases

GeneTreeiENSGT00550000075025.
HOGENOMiHOG000003673.
InParanoidiP28627.
KOiK09647.
OMAiPTHRICK.
OrthoDBiEOG092C52AJ.

Enzyme and pathway databases

BioCyciYEAST:G3O-32841-MONOMER.

Miscellaneous databases

PROiP28627.

Family and domain databases

Gene3Di2.10.109.10. 1 hit.
InterProiIPR026730. Mitochondrial_IMP1.
IPR000223. Pept_S26A_signal_pept_1.
IPR019757. Pept_S26A_signal_pept_1_Lys-AS.
IPR019756. Pept_S26A_signal_pept_1_Ser-AS.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
[Graphical view]
PANTHERiPTHR12383. PTHR12383. 1 hit.
PTHR12383:SF16. PTHR12383:SF16. 1 hit.
PfamiPF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSiPR00727. LEADERPTASE.
SUPFAMiSSF51306. SSF51306. 1 hit.
TIGRFAMsiTIGR02227. sigpep_I_bact. 1 hit.
PROSITEiPS00501. SPASE_I_1. 1 hit.
PS00760. SPASE_I_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMP1_YEAST
AccessioniPrimary (citable) accession number: P28627
Secondary accession number(s): D6VZX2, E9P8W1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 1992
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 141 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.